Fatty Oil Research Articles

Evidence-Based Challenges to the Continued Recommendation and Use of Peroxidatively-Susceptible Polyunsaturated Fatty Acid-Rich Culinary Oils for High-Temperature Frying Practises: Experimental Revelations Focused on Toxic Aldehydic Lipid Oxidation Products.

In this manuscript, a series of research reports focused on dietary lipid oxidation products (LOPs), their toxicities and adverse health effects are critically reviewed in order to present a challenge to the mindset supporting, or strongly supporting, the notion that polyunsaturated fatty acid-laden frying oils are “safe” to use for high-temperature frying practises. The generation, physiological fates, and toxicities of less commonly known or documented LOPs, such as epoxy-fatty acids, are also considered. Primarily, an introduction to the sequential autocatalytic peroxidative degradation of unsaturated fatty acids (UFAs) occurring during frying episodes is described, as are the potential adverse health effects posed by the dietary consumption of aldehydic and other LOP toxins formed. In continuance, statistics on the dietary consumption of fried foods by humans are reviewed, with a special consideration of French fries. Subsequently, estimates of human dietary aldehyde intake are critically explored, which unfortunately are limited to acrolein and other lower homologues such as acetaldehyde and formaldehyde. However, a full update on estimates of quantities derived from fried food sources is provided here. Further items reviewed include the biochemical reactivities, metabolism and volatilities of aldehydic LOPs (the latter of which is of critical importance regarding the adverse health effects mediated by the inhalation of cooking/frying oil fumes); their toxicological actions, including sections focussed on governmental health authority tolerable daily intakes, delivery methods and routes employed for assessing such effects in animal model systems, along with problems encountered with the Cramer classification of such toxins. The mutagenicities, genotoxicities, and carcinogenic potential of aldehydes are then reviewed in some detail, and following this the physiological concentrations of aldehydes and their likely dietary sources are considered. Finally, conclusions from this study are drawn, with special reference to requirements for (1) the establishment of tolerable daily intake (TDI) values for a much wider range of aldehydic LOPs, and (2) the performance of future nutritional and epidemiological trials to explore associations between their dietary intake and the incidence and severity of non-communicable chronic diseases (NCDs).

Extraction and Characterization of Pequi Seed Oil for Biodiesel Production: A Green Management of Waste to Biofuel Using Ethanol and Heterogeneous Catalysis

This work reports the characterization of a vegetable oil extracted from pequi seeds, an agroindustrial residue, and its biodiesel production using ethanol and heterogeneous catalysis. The pequi seeds showed 40.73 wt.% of extractive content, which represents a large amount of the biomass composition. The crude oil extracted from the pequi seeds with ethanol as solvent presented a high content of free fatty acids (FFAs), mainly oleic (54.14%) and palmitic (36.71%) acids, resulting in an acidity value of 13.8 ± 0.1 mg KOH g-1. The esterification/transesterification process was performed using two ion exchange resins as heterogeneous catalysts, a commercial protonic form (assigned as PR) and a zirconium-exchanged (assigned as PRZr). Conversions of 87.1 and 91.4% were achieved for PR and PRZr as catalysts, respectively, at optimal conditions (1:6 oil-to-alcohol molar ratio, 25 wt.% of catalyst, 100 ºC and 1 h). These results indicated that heterogeneous acid catalysts can be successfully applied in biodiesel production from fatty acidrich oils, such as the one extracted from pequi seeds. Also, a simultaneous process involving both oil extraction and biodiesel production was tested using PRZr as catalyst (25 wt.% of catalyst and 100 ºC), but due to the greater amount of ethanol necessary for the oil extraction (1:16 oil to alcohol mass ratio) the conversion reached only 51.5% after 5 h. For that reason, this work proposes a two stage system for biodiesel production that integrates oil extraction (stage one) and the esterification/transesterification reaction (stage two) to achieve a greener process, waste-to-bioenergy.

Enzymatic ethanolysis of high free fatty acid jatropha oil using Eversa Transform

A reduction of the cost of enzymatic biodiesel is crucial to make it competitive with known technologies. A single-step process for the conversion of high free fatty acid oils into fatty acid ethyl esters using a low-cost lipase was developed.

Genetic Regulation of Fatty Acid Biosynthesis in <i>Brassica napus</i> Seeds Based on <i>FAE1</i> and <i>FAD2</i> Genes

Fatty acid elongase 1 (FAE1) and delta-12 fatty acid desaturase 2 (FAD2) are two key enzymes in plant fatty acid biosynthesis pathway. Five different types of transgenic lines with identical genetic background were developed by single and double-expression regulation of the genes coding for these two key enzymes in the same  Brassica napus  variety CY2. Seeds of the five types of transgenic lines and the wild-type control, which were planted under the same environmental conditions, were analyzed and compared for fatty acid composition and oil content in the present study. The results showed that the contents of multiple fatty acids, such as oleic, linoleic, linolenic, eicosenoic and erucic acid, could be significantly altered by single and double regulation of the two genes. Particularly in the seeds in which the two endogenous target genes were simultaneously silenced, the oleic acid content was increased to 82.8% from 20.5% of CY2, and in the seeds with grain-specific expression of  Arabidopsis thaliana   FAE1  gene, the erucic acid content was raised to 60.2% from 43.9% of CY2. Compared with the wild-type control, the relative ratios of eighteen-carbon unsaturated fatty acids and the oleic desaturation proportion were all markedly changed in the five types of transgenic seeds. In addition, it was found that the seed oil content was improved by enhanced expression of  FAE1  and decreased by silencing of  FAE1  and  FAD2 , suggesting that the synthesis and accumulation of seed oil would be influenced to a certain extent by genetic manipulation of the two key enzyme genes.

Using integrated GC-MS analysis, in vitro experiments, network pharmacology: exploring migao fatty oil active components/mechanisms against coronary heart disease

Coronary heart disease (CHD) is one of the main diseases causing morbidity and mortality globally, with oxidative damage and lipid peroxidation associated with oxidative stress and atherosclerosis. Cinnamomum migao is a Miao ethnomedicine that has been shown to provide relief from CHD symptoms. A gas chromatograph-mass spectrometer (GC-MS) was used to determine the chemical components of ten batches of migao fatty oil (MFO), yielding a total of 35 chemical constituents. The antioxidant activity of MFO in vitro was assessed using the DPPH· and ABTS·+ assay methods, and both indicated good antioxidant activity. The network pharmacology predicted the active constituents of MFO and their therapeutic influence on the mechanism of oxidative damage-induced coronary heart disease (OD-CHD). Six compounds were discovered in MFO, including tau-cadinol acetate and dodecanoic acid, which may play a role in OD-CHD by acting on lipids and atherosclerosis, the PI3K-Akt signalling pathway, and other pathways. In conclusion, our study lays the foundation for investigating the use of MFO in the treatment of OD-CHD.

Pharmacognostic Studies of the Leaves and Fructus of Arctium lappa L. (Asteraceae Bercht. & J. Presl)

Introduction: The state quality assurance system ensures the confidence of consumers that the product meets its quality requirements. In this study, pharmacognostic standards of the leaves and fructus of burdock are specified for the first time. Methods: Microscopic analysis was carried out by using the microscope Carl Zeiss. The UV spectrum were measured on an SF-2000 spectrophotometer. Results: Macroscopically, the leaves are heart-shaped, broad-heart-ovate-shaped with a spaced emarginatedserrate or whole edge, with a wide, wedge-shaped, rounded, uneven base, acute apex, green on the outer surface, grayish slightly pubescent on the inner surface. The fructus are obovate or wedge-compressed, slightly curved, the color is brown/black with spots, or variegated with black spots on a gray background. The microscopic examination of the leaves revealed the presence of epidermis, large submerged and non-submerged stomata of the anomocytic type, 2 types of simple unbranched multi-cellular and glandular capitate trichomes; essential oil glands. Microscopic examination of the leaf petiole revealed the presence of 2 types of simple multi-cellular trichomes, epidermis, angular collenchyma, bicollateral bundles, parenchyma. The stomatal index value is 8.28±0.81 per 1cm2. The microscopy of the fructus revealed the presence of elongated cells of the pericarp epidermis; mesocarp; endocarp; endosperm with aleurone grains and drops of fatty oil. The qualitative phytochemical analysis of revealed the presence of polysaccharides, tannins, flavonoids, terpenoids, saponins, phenolic acids. Conclusion: Physical and chemical parameters (moisture, extractive value, ash content) were also specified. These specific data are important for establishing diagnostic indicators for standardization, identification, preparation of new quality standards.

ANALYSIS OF THE QUALITY PARAMETER AND THE FATTY ACID CONTENT FROM THE FOUR OF INDONESIAN CONSUMED NUTS

<p>Consumption of nuts is known to have a variety of health benefits. Nutritional contents in the nuts are needed, especially in order to provide healthy food. Nuts are rich in protein, carbohydrates, fiber, fatty acids, and bioactive compounds. There are four types of beans that are commonly consumed by Indonesian people, namely soybeans [Glycine max (L.) Merr], green beans [Vigna radiata (L.) Wilezek], peas (Pisum sativum L.), and red beans (Phaseolus vulgaris L). The purposes of this study were to produce data on the content of secondary metabolites, the value of quality parameters, and data on fatty acid content in four types of nuts. A series of research phases have been conducted including phytochemical screening, analysis of quality parameters, and analysis of fatty acid content using gas chromatography-mass spectroscopic methods (GCMS). The results showed that the four types of beans contained alkaloids and essential oils, and some of them contained flavonoids, tannins, saponins, and polyphenols. Analysis of quality parameters showed that the content of polar compounds was higher than the semi-polar and non-polar compounds. The largest yield of fatty oil was produced from soybeans (3.83%) followed by peas (1.25%), green beans (0.57%), and kidney beans (0.44%). The oils from the all both contained palmitic acid, LA, and stearic acid. Only green bean and red bean oil contained omega-3, namely ALA, in the amounts respectively 20.37% and 40%. Meanwhile, soybean and peanut oil contained LA 36.71% and 35.53% respectively.</p>

Optimization of biodiesel synthesis from Jojoba oil via supercritical methanol: A response surface methodology approach coupled with genetic algorithm

The most powerful approach to processing biodiesel is supercritical methanol transesterification. This process has several benefits over catalytic transesterification in that it produces a substantial biodiesel production in a brief span of time and removes the problems involved with catalyst processing segregation, and filtration. Besides that, the scMeOH transesterification process necessitates harsh operational exposure to extreme reactants pressure and temperature, as well as a significant volume of methanol. The influence of the key selected parameters (reaction temperature, methanol to oil ratio, reaction time, and pressure) on the production of biodiesel through the supercritical methanol transesterification process was studied using response surface methodology (RSM). As a fuel source, high fatty acid Jojoba oil has been utilized and the transesterification reaction was carried out in a pressure reactor within supercritical conditions. The genetic algorithm (GA) has been employed to optimize biodiesel production. The findings demonstrate that the supercritical methanol transesterification process produced the highest biodiesel production (95.67%) at the optimum conditions of 287 °C reaction temperature, 123 bar reaction pressure, 30:1 methanol to oil ratio, and 23 min reaction time.

The diversity of Lauraceae family in Pu Huong Nature Reserve, Nghe An Province

This paper presents research results on family Lauraceae in Pu Huong Nature Reserve, Nghe An Province. Total 73 species belonging to 13 genera were identified. There were 6 genera and 15 species found as new records for the plant list of Pu Huong published in 2015. Cinnamomum was the richest genus (19 species), then followed by Litsea (17 species), Neolitsea (7 species), and other genera (1 to 6 species). In family Lauraceae of Pu Huong Nature Reserve there were 4 threatened species listed in the Red Data Book of Viet Nam (2007): Cinnamomum parthenoxylon, Cinnamomum balansae, Phoebe macrocarpa and Cinnamomum cambodianum. The useful species of the Lauraceae family is categorized as: 48 timber species, 38 essential oil species, 28 medicinal species, 16 fatty oil and 1 ornamental species. The spectrum of biology of the Lauraceae family in Pu Huong Nature Reserve is summarized as follows: Ph% = 13.70%Mg + 45.20%Me + 38.36%Mi + 1.37%Na + 1.37%Lp. The Lauraceae family in Pu Huong Nature Reserve mainly comprised of the tropical element (39.74%), endemic element (57.52%), temperate element (2.74%).

Strategy to increase the lipid stability of the microbial oil produced by Umbelopsis isabellina for food purposes: Use of microencapsulation by external ionic gelation

Oleaginous microorganisms, including the fungus Umbelopsis isabellina, have emerged as a biotechnological alternative to obtain polyunsaturated fatty acid-rich oils, which are strongly linked to energy purposes (biofuel) than the food industry. Considering the composition of microbial oil and its use by the food industry, it is necessary to investigate strategies that increase its lipid stability. Ergo, this pioneering study aimed to microencapsulate the oil produced by Umbelopsis isabellina and evaluate its oxidative stability throughout the storage period against factors such as temperature and luminosity. The microbial oil was microencapsulated through the external ionic gelation technique, producing an encapsulation efficiency of 80% and proving to be a suitable method because it maintained oil composition. Combining microencapsulation and refrigerated storage led to the best effects on storage time, increasing the evaluated lipid stability through the peroxide values and conjugated diene formation. Moreover, saturated and monounsaturated fatty acid content increased, and polyunsaturated fatty acid content decreased during storage for both the free and microencapsulated oil, regardless of storage temperature, although microencapsulation reduced the changes. The results primarily demonstrate how microencapsulation prolongs the oxidative stability and unsaturated fatty acid content of the microbial oil by reducing its reactions to external environmental factors, thus facilitating its use in the food industry.

THE INFLUENCE OF THE CLIMATIC CONDITIONS OF THE REPUBLIC OF DAGESTAN ON THE CONTENT OF FATTY OIL AND OTHER BIOLOGICALLY ACTIVE SUBSTANCES IN THE FRUITS OF MILK THISTLE SPOTTED

The object of the study was the fruits of milk thistle [Silybum marianum (L) Gaertn.] and fatty oil obtained from them by hexane extraction in the Soxlet apparatus.
 The aim of the study was to study the influence of natural and climatic conditions on the accumulation of fatty oil and other biologically active substances in the fruits of milk thistle growing on the territory of the Republic of Dagestan.
 It was found that the content of fatty oil and proteins in the fruits of milk thistle increases with a decrease in air temperature, an increase in the amount of precipitation and soil moisture at the place of growth; the content of carbohydrates and flavolignans in the studied samples of milk thistle fruits is inversely dependent on the content of fatty oil.
 It was revealed that with an increase in the amount of precipitation and a decrease in the amount of active temperatures during the change of the natural and climatic zone from the foothill Kaitagsky to the high-mountain Kulinsky district, the total content of unsaturated fatty acids, primarily linoleic acid, in milk thistle oil samples decreases.
 The method of differential scanning calorimetry has been used to study the thermophysical properties of the fatty oil of milk thistle growing in the Kulinsky, Levashinsky and Kaitagsky districts of Dagestan. The results of the thermal analysis are in good agreement with the conclusions obtained from the results of chemical and chromatographic analysis, differential scanning calorimetry allows us to identify trends in the change in the fatty acid and triglyceride composition of milk thistle fatty oil, depending on the natural and climatic conditions of the growth of milk thistle.

A Tree Peony Trihelix Transcription Factor PrASIL1 Represses Seed Oil Accumulation.

In many higher plants, seed oil accumulation is governed by complex multilevel regulatory networks including transcriptional regulation, which primarily affects fatty acid biosynthesis. Tree peony (Paeonia rockii), a perennial deciduous shrub endemic to China is notable for its seed oil that is abundant in unsaturated fatty acids. We discovered that a tree peony trihelix transcription factor, PrASIL1, localized in the nucleus, is expressed predominantly in developing seeds during maturation. Ectopic overexpression of PrASIL1 in Nicotiana benthamiana leaf tissue and Arabidopsis thaliana seeds significantly reduced total fatty acids and altered the fatty acid composition. These changes were in turn associated with the decreased expression of multitudinous genes involved in plastidial fatty acid synthesis and oil accumulation. Thus, we inferred that PrASIL1 is a critical transcription factor that represses oil accumulation by down-regulating numerous key genes during seed oil biosynthesis. In contrary, up-regulation of oil biosynthesis genes and a significant increase in total lipids and several major fatty acids were observed in PrASIL1-silenced tree peony leaves. Together, these results provide insights into the role of trihelix transcription factor PrASIL1 in controlling seed oil accumulation. PrASIL1 can be targeted potentially for oil enhancement in tree peony and other crops through gene manipulation.

Antioxidant, Antimicrobial Activities and Fatty Acid Compositions of Wild Berberis spp. by Different Techniques Combined with Chemometrics (PCA and HCA).

Interest in medicinal plants and fruits has increased in recent years due to people beginning to consume natural foods. This study aims to investigate the total phenolic flavonoid content, antioxidant activity, condensed tannin content, oil content, and fatty acid compositions of five local breeds of Berberis spp. from Bayburt, Turkey, and their antioxidant and antimicrobial activities. The fatty acid composition of samples was performed with gas chromatography-mass spectrometry (GC-MS), and the total fatty acid content of samples was between 6.12% and 8.60%. The main fatty acids in Berberis spp. samples were α-linolenic acid (32.85–37.88%) and linoleic acid (30.98–34.28%) followed by oleic acid (12.85–19.56%). Two antioxidant assays produced similar results, demonstrating that extracts of wild B. vulgaris L. had the highest ferric reducing antioxidant power (FRAP) (621.02 μmol FeSO4.7H2O/g) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) (0.10 SC50 mg/mL) values. According to principal component analysis (PCA), four components were determined. In addition, two main groups were determined according to hierarchical cluster analysis (HCA), and wild and culture of B. vulgaris L. were in different subgroups. This is the first original report about the fatty acid composition and oil content of Berberis spp. grown in Bayburt, Turkey. The obtained results indicate that B. integerrima Bunge and B. vulgaris, which have especially remarkable fatty acid content, antioxidant, and antimicrobial activity, could be potential sources for these properties in different areas of use.

Chemical Studies on Fatty Oil of Trewia nudiflora Kernels

Abstract-Trewia nudiflora Linn belongs to family Euphorbiaceae, sub-family Acalyphoideae and it is one of the important medicinal plants in Indian systems of medicine like Ayurveda, Siddha, etc. It has numerous phytochemical and pharmacological significance. The kernels ofTrewia nudiflorawere chemically examinedand fatty oil was isolated and characterized by Gas Liquid Chromatography. The fatty oil content in the kernels was found to be 20.99% on moisture free basis. Arachidic acid (40.25%) and Linolenic acid (30.26%) were identified as major constituent. Physico-chemical analysis of fatty oil was also carried out.

Crystal network structure and stability of beeswax-based oleogels with different polyunsaturated fatty acid oils

The effect of different types of oils including camellia oil (CLO), sunflower oil (SFO), corn oil (CO) and linseed oil (LO) on the formation, crystal network structure and mechanical properties of 4%wt beeswax (BW) in oleogel was investigated. BW oleogels containing oils with higher contents of polyunsaturated fatty acids gelled first (1%wt), especially LO with higher contents of linolenic acid rather than CLO with higher contents of monounsaturated fatty acids. In comparison, oils with higher polyunsaturated fatty acid contents exhibited higher Db with more extensive microstructure at different cooling rates, which was related to shorter nucleation induction time of crystal and higher crystallinity. Stronger van der Waals forces were observed in oleogels with higher polyunsaturated fatty acid contents especially for LO oleogel. Rheology also showed that LO oleogel with higher content of linolenic acid had higher crystallinity and lower crystal melting interfacial tension, resulting in the formation of a more stable network structure.

Fish oil enriched ω-3 fatty acids ameliorates protein synthesis/degradation imbalance, inflammation, and wasting in muscles of diet-induced obese rats

• Effects of fish oil (FO) on muscles in high-fat diet-induced obese rats were tested. • Supplementation with 5% FO for 16 weeks prevented obesity and skeletal muscle loss. • FO prevented muscle wasting via activating AKT/mTOR and AMPK/PGC-1α signals. • FO improved protein synthesis/degradation imbalance and wasting in obese rat muscle. We investigated the effects of long-term supplementation of omega-3 fatty acids-enriched fish oil (FO) on preserving muscle mass. We found that dietary supplementation with 5% FO has a direct impact on preventing obesity and skeletal muscle loss in rats fed a high-fat diet for 16 weeks. The increased protein expression for ubiquitin ligases atrogin-1 and muscle RING-finger protein 1 (MuRF1) and the autophagy-related protein, PTEN-induced putative kinase 1 (PINK1) in muscles were both abrogated by FO. The AKT and mammalian target of rapamycin (mTOR) phosphorylation and the AMP‐activated protein kinase (AMPK)/peroxisome proliferator‐activated receptor γ coactivator 1α (PGC‐1α) signaling activation in muscles were up-regulated by FO. FO supplementation effectively reversed the increased plasma TNF-α levels and NF‐κB activation in muscles. These results suggest that FO supplementation prevents skeletal muscle wasting under obesity through activating AKT/mTOR and AMPK/PGC-1α signals and blocking NF-κB-mediated inflammatory pathway to improve the balance between protein synthesis and degradation.

Synthesis and Characterization of Polyurethanes from Residual Palm Oil with High Poly-Unsaturated Fatty Acid Oils as Additive.

In the effort to produce renewable and biodegradable polymers, more studies are being undertaken to explore environmentally friendly sources to replace petroleum-based sources. The oil palm industry is not only the biggest vegetable-oil producer from crops but also one the biggest producers of residual oil that cannot be used for edible purposes due to its low quality. In this paper the development of biopolymers from residual palm oil, residual palm oil with 10% jatropha oil, and residual palm oil with 10% algae oil as additives were explored. Polyols from the different oils were prepared by epoxydation with peroxyacetic acid and alcoholysis under the same conditions and further reacted with poly isocyanate to form polyurethanes. Epoxidized oils, polyols and polyurethanes were analyzed by different techniques such as TGA, DSC, DMA, FTIR and H-NMR. Overall, although the IV of algae oil is slightly higher than that of jatropha oil, the usage of algae oil as additive into the residual palm oil was shown to significantly increase the hard segments and thermal stability of the bio polyurethane compared to the polymer with jatropha oil. Furthermore, when algae oil was mixed with the residual palm oil, it was possible to identify phosphate groups in the polyol which might enhance the fire-retardant properties of the final biopolymer.

Extraction of Fatty Oil from Milk Thistle Fruits with Supercritical Carbon Dioxide

Extraction of Fatty Oil from Milk Thistle Fruits with Supercritical Carbon Dioxide

Pengolahan Limbah Cair Rumah Sakit dengan Metode Lahan Basah Buatan (Constructed Wetland) dan Tanaman Air Typha latifolia

<p>Utilization of the aquatic plant Typha latifolia, in the treatment of hospital wastewater with the method of an artificial wetland system (constructed wetland), is expected to be able to treat the liquid waste of health care facilities. This study aims to determine the ability of the aquatic plant Typha latifolia in degrading the parameters contained in the wastewater of health facilities using the Artificial Wetland Method. Quasi-experimental research design. The measurement of liquid waste parameters (BOD, COD, TSS, Fatty Oil, and Ammonia) is carried out by taking samples at the inlet and outlet of each pot, then analyzed in the laboratory. The study reported an average influent BOD level of 66.2mg/L, an average effluent in a circular cross-sectional pot increased by 99.6mg/L, an influent COD level of 190.8mg/L, an effluent in a rectangular pot four decreased by an average of 31.6mg/L, the influent TSS parameter was 106mg/L, the average effluent in a circular cross-sectional pot was 283.6mg/L, the influent Oil & Fat parameter averaged 1.2mg/L, average effluent 1.2mg/L, ammonia influent parameter 12mg/L, effluent 2.62mg/L. The rectangular cross-section has a good ability compared to other cross-sections in reducing hospital waste, namely; BOD=64%, COD=40%, TSS=48%, Ammonia=67%, Oil and Fat parameters did not decrease because the influent parameter levels were already below the Liquid Waste quality standard. The researcher concluded that the rectangular cross-sectional pot planted with Typha latifolia could reduce the wastewater parameters better than other cross-sectional types. </p>

Biodiesel production from Bauhinia variegata seeds oil using homogeneous catalyst

At present, a large amount of biodiesel is produced from the non-edible and waste cooking oils using alcohol and catalyst (acid, alkaline). The free fatty acids in the oils react fast with the alkaline catalyst to produce soap that inhibits the reaction. In the present work, a two-step trans-esterification process is developed to convert the high free fatty acid oils to its mono-esters. In the first step, acid catalyzed esterification reduces the free fatty acid level of the oil to less than 1.9%, and the alkaline catalyzed trans-esterification process converts the yields of the first step to its mono-esters and glycerol during the second step. The main factors such as alcohol and oil a molar ratio, catalyst amount, reaction time and temperature are optimized. The property of biodiesel obtained is similar to that of petro-diesel.