Sage Extracts Research Articles

Liquid crystal biomimetic nanosystems loaded with vegetal extracts

This article is aimed to design new antioxidant phyto-nanosystems based on liposomes and vegetal extracts of sage (Salvia officinalis L.) and mint (Mentha piperita L.). The lipid vesicles were obtained by thin film hydration method, and vegetal extracts of sage or mint were incorporated both in hydrophobic and in aqueous compartments of these lipid systems. The obtained phyto-containing liposomes were characterized by UV–Vis absorption and dynamic light scattering (DLS) measurements, and their antioxidant activity was assessed through chemiluminescence technique. The obtained artificial lipid bilayers loaded with phyto-extracts exhibited impressive antioxidant properties.

Evaluation of Singlet Oxygen Scavenging Capacity of Peppermint (Mentha Piperita L.), Marjoram (Origanum Majorana L.), Rosemary (Rosmarinus Officinalis L.) And Sage (Salvia Officinalis L.) on Fatty Acid Photooxidation

Lipid photooxidation is the undesirable chemical process in which singlet oxygen result in the peroxidation of fatty acids. In this study leaves methanolic extracts of peppermint (Mentha piperita L.), marjoram (Origanum majorana L.), rosemary (Rosmarinus officinalis L.) and sage (Salvia officinalis L.) were applied as the natural singlet oxygen scavenger. Amount of flavonoid compounds as the singlet oxygen scavenger agent in these plant species were decreased in the order of peppermint > marjoram> sage > rosemary. Also, The rate of quenching of singlet oxygen in the presence of 1,4-Diazabicyclo[2.2.2]octane (DABCO) as a well-known singlet oxygen scavenger and highly effective synthetic antioxidants in food industry such as Butylated hydroxyanisole (BHA), tert-Butylhydroquinone (TBHQ) and peppermint decreased in the order of peppermint > BHA > TBHQ > DABCO >. Furthermore, photooxidation of oleic acid as an unsaturated fatty acid in the presence of DABCO, peppermint, BHA and TBHQ indicated a preservation of 82.77%, 73.39%, 71.57% and 53.10% on peroxidation of oleic acid, respectively which reveals peppermint has an efficient role on protection of fatty acids from photooxidation. Practical application: In this study, it was confirmed that peppermint (Mentha piperita L.) performs an effective role in restricting or limitation of singlet oxygen generation and fatty acid photooxidation. In vitro study of scavenging effect of peppermint can correlate laboratory results to commercial scale up. However, this would also necessitate the progress of improved methods for the measurement of lipid peroxidation in vivo in the presence of peppermint.

HPTLC based approach for bioassay-guided evaluation of antidiabetic and neuroprotective effects of eight essential oils of the Lamiaceae family plants

A high-performance thin-layer chromatography (HPTLC) method combined with effect-directed-analysis (EDA) was developed to screen the antioxidant, neuroprotective and antidiabetic effects in essential oils derived from lavender flower, lemon myrtle, oregano, peppermint, sage, and rosemary leaves (Lamiaceae family). HPTLC hyphenated with microchemical (DPPH•, p-anisaldehyde, and ferric chloride) derivatizations, was used to evaluate antioxidant activity, presence of phytosterols and terpenoids, and polyphenolic content, while the combination with biochemical (α-amylase and acetylcholine esterase (AChE) enzymatic) derivatizations was used to asses α-amylase and AChE inhibitory activities. The superior antioxidant activity of oregano leaf extract is attributed to the presence of high levels of aromatic compounds, like polyphenolic acids. The strongest α-amylase inhibition was observed in lemon myrtle and rosemary plus extracts due to the presence of monoterpenes. Rosemary and sage extracts exhibit the highest AChE inhibition activity, with 1 μL essential oils being more potent than the recommended daily dose of donepezil. This superior neuroprotection was attributed to the presences of di- and triterpenes that displayed strong AChE inhibition and antioxidant potential in DPPH• free radical assay. Antioxidant activity was related to phenolic content (R = 0.49), while α-amylase inhibitory activity was positively related to antioxidant activity (R = 0.20) and terpenoid/sterol content (R = 0.31). AChE inhibitory activity was correlated (R = 0.80) to the combined effect of phenolics and terpenoids. Thus, the superior AChE inhibitory and neuroprotection potential of rosemary and sage essential oils could be attributed to joint effects of main phenolic and terpene constituents. The hyphenated HPTLC method provided rapid bioanalytical profiling of highly complex essential oil samples.

The use of bioactive properties of sage preparations to improve the storage stability of low-pressure mechanically separated meat from chickens

The aim of this study was to determine the effect of Salvia officinalis L. (sage) preparations on the storage stability of vacuum-packed low-pressure mechanically separated meat (MSM) from chickens stored at -18°C for 9 mo. Chilled low-pressure MSM coming from 4 production batches was obtained from the plant located in north-eastern Poland. All sage preparations-a water extract, ethanol extracts, and an essential oil-were prepared and added to the MSM under laboratory conditions. Five samples of low-pressure MSM were prepared that differed in terms of the type of sage preparation added: control-without addition of sage, WE-2.0% addition of water extract from sage, E40-2.0% addition of 40% (vol/vol) ethanol extract from sage, E70-2.0% addition of 70% (vol/vol) ethanol extract from sage, and EOS-0.1% addition of essential oil from sage. The MSM samples were assessed immediately after arrival into the laboratory and after 1, 2, 3, 4, 6, and 9 mo of storage. The quality changes of MSM samples were determined on the basis of the thiobarbituric acid reactive substances (TBARS) index and microbial analyses. On the basis of the TBARS index it was proved that addition of essential oil and (40% and 70% (vol/vol)) as well as water extracts of sage significantly (P < 0.05) slowed down fat oxidation processes in low-pressure MSM from chickens. The growth of mesophilic aerobic bacteria and psychrotrophic bacteria, coliforms, and Enterobacteriaceae was significantly (P < 0.05) restricted by all sage preparations tested. The most effective inhibitory effect for groups of bacteria analyzed in this study was demonstrated by sage essential oil, despite insignificant (P > 0.05) differences between the preparations. In conclusion, it was found that sage preparations are rich in bioactive compounds. Due to their antioxidant and antibacterial properties, especially sage oil and ethanol extracts may be recommended as an auxiliary factor to prolong the storage stability of frozen, vacuum-packed low-pressure MSM from chickens.

Ocena oddziaływania na grzyby drożdżopodobne olejku szałwiowego (Oleum Salviae)

Introduction. The essential oils were known and used in ancient Times. The oil and extracts of sage are utilized in folk medicine and for food condiment, in cosmetics and perfumes. Sage (Salvia officinalis) belonging to the Labiatae family. Plant grow all over the world. Sage has a number of properties in it antiflogistic and antimicrobial. The oil is used for the treatment of different kind of diseases, including bronchitis, cough, arthritis, rheumatism, ulcers, inflammation of skin, alimentary tract and in Alzheimer’s disease. Etheric oil contain following compounds: α- and β-thujone, 1,8-cineole, camphor, borneol, α-pinene, β-pinene, β-caryophyllene, β-sabinene, limonene, α-humulene, myrcene, α-terpineol, viridiflorol and camphene. The chemical compounds of the oil have antioxidant and antimicrobial properties. Aim. The aim of the dates was to evaluate the susceptibility of yeastlike fungi to sage. Material and methods. A total 30 strains of yeastlike fungi isolated from patients with oral candidosis was tested. The strains were identified with system API 20 C AUX (BioMèrieux), production chlamydospore and pseudohyphe. The susceptibility (MIC) yeastlike fungi to sage oil was determined by means plate dilution technique in Sabouraud’s agar. The suspension contained 105 CFU per spot were spread Steers replicator over the surface of agar containing oil or without sage agar plates (strains growth control). Inoculated agar plates were incubated in aerobic conditions at 37°C for 24-48 hrs. The MIC was defined as a lowest concentration of the oil inhibited growth of fungal strains. Results. The results indicated that the sage oil was active against yeastlike fungi in concentrations 0.5-≥ 2.0 mg/ml. The MIC for 66% strains from genus Candida albicans ≥ 2.0 mg/ml. Similarly C. krusei strains were susceptible in range 0.5-≥ 2.0 mg/ml. The oil was less active towards C. glabrata and C. tropicalis strains (MIC 1.0-≥ 2.0 mg/ml). The most susceptible were the strains from genus of C. parapsilosis. The growth was inhibited within the range from 0.5 to 1.0 mg/ml. The strain from genus of Rhodotorula rubra was susceptible on 0.12-0.5 mg/ml and Saccharomyces cerevisiae on 0.25 mg/ml. Conclusions. Sage oil showed antifungal activity. The more susceptible to oil were the strains of Candida parapsilosis, Rhodotorula rubra and Saccharomyces cerevisiae. Oil was less active toward strains Candida glabrata, Candida tropicalis and Geotrichum candidum.

Comparative study of the antioxidant and antibacterial activities of the essential oil and methanolic extract of cultivated sage from Mascara region (North West of Algeria) indicates a strong antioxidant activities and an important antibacterial action wi

The plant world is an excellent source of active principles, which confers its important activity. In Algeria, a large number of aromatic and medicinal plants grow spontaneously, common sage (Salvia officinalis) is a popular culinary and medicinal herb. These natural products and their active principles are sources for new drug discovery and treatment of diseases. To this end, the interest of this work is the valorization of a medicinal plant (Salvia officinalis) growing spontaneously in the region of Mascara by the study of antioxidant and antibacterial activities of the methanolic extracts and also essential oils of sage. The essential oil was isolated from aerial part of S. officinalis by hydrodistillation. The results obtained showed that the species studied (S. officinalis) was very rich in volatile substances with a yield of the order of1.19%. The antioxidant activity showed a good efficiency of the methanolicextract with respect to the essential oil with IC50s of 0.778 mg / ml and27.929 mg / ml respectively. In general, the essential oil of S. officinalis hasan interesting antibacterial power against all studied germs. These resultsare promising and provide scientific validation of the massive use of this species.

Enterocin M and Sage Supplementation in Post-weaning Rabbits: Effects on Growth Performance, Caecal Microbiota, Fermentation and Enzymatic Activity.

The effects of enterocin (Ent) M and sage extract applied separately and in combination were investigated. EntM (E 50μL/animal/day in water) and sage extract (S 10μL/animal/day in water) were applied individually and in combination (E+S) to rabbits during 21days of treatment. The rabbits' growth was not significantly influenced by the additives. Lower feed conversion (FC) was noted in the experimental groups compared with controls, with the lowest data detected in E. The antimicrobial activity of EntM was noted (in E+S: lactic acid bacteria-P < 0.01; in E, E+S: enterococci, enterobacteria-P > 0.05; in E: clostridia-P > 0.05). The most significant changes in fermentation between weaned and older rabbits were noted in amylolytic activity at day 21 (E P < 0.05; E + S P < 0.05); prolonged reduction effect of sage extract on amylolytic activity was observed. The activity of cellulase, pectinase and xylanase was higher in older than in younger animals. Decrease in lactic acid and volatile fatty acids was noted during EntM administration, with significant effect on propionic acid concentration (E P < 0.05; E+S P < 0.001). The sage extract reduced propionic acid (S P < 0.001) and butyric acid levels (S P < 0.05) and increased the concentrations of butyric, iso-valeric, valeric, caproic acids and lactic acid (P < 0.001). It seems to be that EntM and sage supplementation may improve the economy of rabbit farms (increased FC) and the health status of rabbits (reduction of spoilage microbiota, enhanced enzymatic activities in caecum).

The effect of Saliva officinalis extract on the menopausal symptoms in postmenopausal women: An RCT.

BackgroundThe menopausal symptoms are the most common problems in postmenopausal women. Due to the side effects of hormone replacement therapy, the use of medicinal herbs has increased for the treatment of menopausal symptoms.ObjectiveThe aim of this study was to evaluate the effect of Saliva officinalis on the decreasing of the severity of the menopausal symptoms in postmenopausal women.Materials and MethodsThe study was performed on 30 postmenopausal women aged 46–58 yr referred to the healthcare center of Darab who experienced various degrees of postmenopausal symptoms. The severity of menopausal symptoms is recorded by a Menopause Rating Scale. Participants received a 100 mg capsule of sage extract daily for 4 wk. The severity of postmenopausal symptoms was compared before and after four weeks of the consumption of sage extract.ResultsThe results showed the severity of hot flashes, night sweats, panic, fatigue, and concentration had significant differences before and after the consumption of sage extract.ConclusionIt was concluded that Saliva officinalis were effective to change the severity of some of the menopausal symptoms in postmenopausal women.

Biologically valuable components, antioxidant activity and proteinase inhibition activity of leaf and callus extracts of Salvia sp.

Abstract Sage is medicinal plant, known for its antioxidant and anti-inflammatory effects. Eight extract samples were tested in this study: extract from Salvia officinalis L. varieties from two different geographical localities (Jaslovské Bohunice and Pobedim, Slovakia), Salvia officinalis L., variety “bicolor”, Salvia officinalis L., variety “purpurescens”, Salvia apiana, Salvia divinorum, and two callus cultures of Salvia sclarea L. and Salvia aethiopis L. The highest values for composite parameters were observed for extract from Salvia apiana. It can be concluded that prepared sage extract samples are rich on polyphenolic acids (2 950±265 μg.mL−1 GAeq.) and amines (197±5.50 μg.mL−1 TRPeq.). HPLC analysis confirmed the dominant content of rosmarinic acid in the extracts; the highest content was detected in the Salvia apiana extract (1 120±15 μg.mL−1). Extract from Salvia apiana expressed too the highest antioxidant activity (1 710 – 4 669 μg.mL−1TEAC). Similarly, the highest inhibition activity was observed for this extract on thrombin (57±3.3 %) and on other proteinases (over 80 %). Spearman correlation analysis and PCA analyses revealed a coherence between antioxidant activity of samples and their content of rosmarinic acid as well as inhibitory activity towards particular proteases, and revealed the significance of thiol based secondary metabolites. Cluster analysis demonstrates the differences of Salvia apiana extract from extracts of S. officinalis L., the group of S. divinorum extract and from callus cultures.

Effect of sage (Salvia officinalis L.) aqueous leaf extract on performance, blood constituents, immunity response and ileal microflora of broiler chickens

This study was aimed to evaluate the effect of different levels of sage (Salvia officinalis L.) aqueous leaf extract on performance, blood parameters, immunity response and ileal microflora in broiler chickens. A total of 300 male Ross-308 chicks were used until 42 days of age. Birds were randomly divided into five groups (three replicates with 20 birds per replicate) The treatments consisted of a control diet without feed additive and experimental diets supplemented with four levels of sage extract in drinking water (0, 100, 200, 300 and 400 ppm, respectively). Based on results, with the exception of significant differences between groups I and II regarding growth performance, in overall no differences among treatments were observed on body weight gain, feed intake and feed conversion ratio. Blood analysis showed that broilers fed different levels of sage extracts decreased (P < 0.05) plasma total cholesterol, triglycerides and low-density lipoprotein and increased high-density lipoprotein. A significant (P < 0.05) enhancement of the immunity response of broilers was observed when the dietary concentration of sage extract increased. Further, a significant (P < 0.05) bactericidal effect of sage extract was detected for Escherichia coli, whereas it was moderate for Lactobacillus. In conclusion, the positive effects sage extract on broilers immunity parameters and antibacterial activity were found to be strictly related with the dietary inclusion level.

Effects of Salvia Officinalis Extract on the Breast Cancer Cell Line

Background: Common sage (Salvia officinalis L., Lamiaceae) is an aromatic and medicinal plant well known for its antioxidant properties. This plant belongs to Lamiaceae family and has many pharmaceutical properties. Some in vivo studies have shown the biological antioxidant effects of sage. As a member of Salvia officinalis Labiatae, sage is also known as “Maryam flower” in Iran. Sage importance lies in its therapeutic potential. It has been exploited as an anti-spasmodic, astringent, sedative, anti-hyperglycemic, and anti-inflammatory agent in Iranian medicine. Objective: Studying the anticancer effects of the compounds in Salvia officinalis extracts, such as cineol and camphor. Methods: Cancer was induced by DMBA (dimethyl-benzantheracene) dissolved in sunflower oil for 4 weeks. The case group was treated with sage leaf hydroalcoholic extract for 4 weeks; while the controls received distilled water. Result: Angiogenesis is a key process in cancer spread and metastasis. The hydroalcoholic extract of garden sage halted angiogenesis in the breast cell line of both human and mouse models; the highest impact was observed in hexane extract. Findings indicated the therapeutic effects of garden sage (i.e. its in vitro anti-angiogenesis activity and anti-migratory properties). Conclusion: Saliva officinalis can potentially prevent breast cancer.

Physicochemical, Antioxidant and Antimicrobial Properties of Electrospun Poly(ε-caprolactone) Films Containing a Solid Dispersion of Sage (Salvia officinalis L.) Extract.

In this study, novel active films made of poly(ε-caprolactone) (PCL) containing a solid dispersion of sage extract (SE) were developed by means of the electrospinning technique and subsequent annealing treatment. Initially, the antioxidant and antimicrobial potential of SE was confirmed. Thereafter, the effect of SE incorporation at different loading contents (5%, 10%, and 20%) on the physicochemical and functional properties of the films was evaluated. The films were characterized in terms of morphology, transparency, water contact angle, thermal stability, tensile properties, water vapor, and aroma barrier performances, as well as antioxidant and antimicrobial activities. Thin, hydrophobic films with good contact transparency were produced by annealing of the ultrathin electrospun fibers. Interestingly, the effect of SE addition on tensile properties and thermal stability of the films was negligible. In general, the water vapor and aroma permeability of the PCL-based films increased by adding SE to the polymer. Nevertheless, a strong 2,2-diphenyl-1-picrylhydrazyl (DPPH·) free radical scavenging ability, and a strong activity against foodborne pathogens Staphylococcus aureus and Escherichia coli were achieved by SE incorporation into PCL matrix. Overall, the obtained results suggest great potential of the here-developed PCL-based films containing SE in active food packaging applications with the role of preventing oxidation processes and microbial growth.

Supercritical CO2 extraction of Salvia fruticosa

The supercritical fluid extraction (SFE) of Greek sage leaves, i.e. Salvia fruticosa, was investigated at various operation conditions. Specifically, the effect of pressure, temperature and solvent flow rate on the extraction yield was examined. Thus, it was shown that as pressure increases, yield increases as well, while the effect of temperature depends on the extraction pressure. As for the flow rate, its increase mainly enhances the extraction rate. The SFE extracts were also compared with the essential oil obtained with hydrodistillation in terms of composition, revealing practically the absence of thujone and the presence of more biologically active compounds in SFE extracts. Finally, the experimental data were correlated successfully by a mass balance model based on Lack’s plug flow model.

Extraction of Carnosic Acid and Carnosol from Sage (Salvia officinalis L.) Leaves by Supercritical Fluid Extraction and Their Antioxidant and Antibacterial Activity.

Sage (Salvia officinalis L.) is a good source of antioxidant compounds, carnosic acid and carnosol being the prominent ones. Both are soluble in CO2, and our goal was to investigate the application of supercritical CO2 extraction to obtain sage extracts rich in these compounds. The effect of pressure, temperature, and CO2 flow rate on the carnosic acid and carnosol yield was studied. These variables were optimized by response surface methodology (RSM). The pressure significantly affected carnosol extraction, while the extraction of carnosic acid was affected by the pressure, temperature, and CO2 flow rate. Carnosic acid content varied from 0.29–120.0 µg mg−1, and carnosol content from 0.46–65.5 µg mg−1. The optimal conditions according to RSM were a pressure of 29.5 MPa, a temperature of 49.1 °C, and a CO2 flow rate of 3 kg h−1, and the sage extract yield was calculated to be 6.54%, carnosic acid content 105 µg mg−1, and carnosol content 56.3 µg mg−1. The antioxidant activities of the sage extracts were evaluated by the scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH). Sage extract obtained at 30 MPa and 40 °C with 2 kg h−1 CO2 flow rate with a carnosic acid content of 72 µg mg−1 and carnosol content of 55 µg mg−1 exhibited the highest antioxidant activity (80.0 ± 0.68%) amongst the investigated supercritical fluid extracts at 25 µg mL−1 concentration. The antimicrobial properties of extracts were tested on four bacterial strains: Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus. The extract with a carnosic acid content of 116 µg mg−1 and a carnosol content of 60.6 µg mg−1 was found to be the most potent agent against B. subtilis.

Diurnal variation of the chemical composition and its repercussion on the biological activity polyphenolics of Salvia officinalis aerial parts

This study reported the diurnal variation of the chemical composition and biological activities of phenolic compounds of Salvia officinalis cultivated in Tunisia. The HPLC determination of different phenolic compounds showed that the amounts of total polyphenols reach its maximum at 7 am. Estimation of the antioxidant activity of different methanolic extracts of Salvia officinalis showed that the extract of 5 pm had the most effective antiradical power against DPPH and the best iron reducing capacity. The strongest total antioxidant capacity was recorded for extracts of 12 and 5 pm. The methanol extracts of sage also have a capacity to reduce the appetence of Spodoptera littoralis larvae. This activity did not vary with the harvesting time.

Antioxidant Activity and Biological Studies of Two Medicinal Plant Extracts on Spodoptera littoralis (Boisd)

Control management of Spodoptera littoralis (Boisd) infestation has been achieved by using chemical insecticides. However, the environment-friendly methods without unwanted side effects of these chemicals are becoming very important in modern pest management strategies. Natural products including plant ethanolic extracts and essential oils are some of the alternative approaches in pest control. The essential oils were extracted and their chemical composition was identified using a GC/MS spectrometer. Therefore, the present study was designed to evaluate the antioxidant activity of various extracts of sage and thyme plants by 2, 2-diphenyl-1-picrylhydrazil (DPPH) method. The obtained results revealed that concentrations at 25, 50, 100 and 200µg/ml of essential oils and ethanolic extract from both plants were more efficient in scavenging free radicals after 30 min when compared with butyl hydroxyl toluene(BHT) as synthetic standard. The concentrations 100 and 200µg/mL from ethanolic and essential oils were having higher activity than other extracts and standard .Furthermore, Sage and thyme extracts were investigated for their toxicity against cotton leafworm, S. littoralis in the laboratory, the percentages of cumulative mortality of larvae as well as the latent effects of the 4th instar of S. littoralis four concentrations (25, 50, 100 and 200 µg/mL) were applied. It was found that ethanolic extract of two plants was more efficient than essential oils on different stages of S. littoralis. The results showed that there were highly significant differences between all treatments and control some biological aspects.

Studying the Affects of Salvia officinalis and Commiphora myrrha Extracts on Poly Methyl Methacrylate Acrylic (PMMA) and Flexible Acrylic Materials Exposed to Escherichia coli

Myrrha and Sage extract solutions are promising prophylactic and disinfectant agents against microorganisms, suggesting that these solutions might be useful as antimicrobial topical solutions for dental contamination. Three different concentrations of Myrrha and Sage extract solutions 2%, 5% and 10% were separately exposed to the E.coli and chlorohexidine solution that used as control to treat the PMMA and Flexible Acrylic plates, the percentage of colonies reductions were calculated. In case of using Sage extract solutions, the colonies reductions were 82.70%, 90.65% and 94.61% respectively for PMMA plates compared with 97.86% reduction for Chlorohexidine with mean p-value 0.0023, 0.0025, 0.0026 and 0.0009 respectively. As regard Myrrha extract solutions, the colonies reductions for Acrylic flexible plates were 91.75%, 96.15%, 97.08% respectively, compared with 97.91% reduction for Chlorohexidine with mean p-value (0.0017, 0.0010, 0.0005 and 0.0006) respectively. This research considers both Sage and Myrrha and extract solutions of different plants are promising solutions and capable of using them as alternative anti plaque solutions for either PMMA and Flexible Acrylic plates.

Utjecaj dodatka antioksidanasa na oksidacijsku stabilnost goveđeg loja

Influence of antioxidants on oxidative stability of beef tallow Fats are contained in the majority of foods that are consumed daily. Beef tallow is one of the most complex fats found in nature. Tallow is subject to oxidation during the production, storage and heat treatment. This study researched the effect of natural antioxidants (rosemary extract type Oxy’Less CS and type StabilEnhance, sage extract, alpha tocopherol, mixture tocopherol) and synthetic antioxidants (PG, BHA) on the oxidative stability of beef tallow. The oxidative stability of beef tallow, with and without added antioxidant, was evaluated using the sustainability test at 98 oC. The results are expressed as peroxide value (mmol O2/kg) obtained after storing the sample for a certain period of time at the temperature of 98°C. The results showed that applied antioxidants successfully stabilized the beef tallow. Among natural antioxidants, rosemary extract type Oxy'Less CS showed a higher antioxidant activity in beef tallow. In comparison with other tested antioxidants, it achieved greater efficiency in protecting the beef tallow from oxidation. Synthetic antioxidants propyl galate and butylhydroxyanisole successfully increased the stability of beef tallow, whereby propyl galate showed a higher antioxidant activity.

New Simple Media Substrates to Differentiate &amp;lt;i&amp;gt;Candida dubliniensis&amp;lt;/i&amp;gt; from &amp;lt;i&amp;gt;Candida albicans&amp;lt;/i&amp;gt;

Candida dubliniensis is a recently described as an important pathogenic species, which shares many phenotypic features with Candida albicans and therefore microbiological laboratories may be misidentified. The molecular methods can be exhausting and unavailable at many mycological laboratories, with restricted budgets such as those in developing countries. Therefore, the phenotypic techniques have been encouraged in the development of simple differential media with high specificity and sensitivity for the presumptive identification of these species. We examined the colony morphology and chlamydospore production of C. dubliniensis (28 isolates) and C. albicans (40 isolates). Isolates were cultured on several new proposed media such as Coriander (Coriandrum sativum L), Cumin (Cuminum cyminum), Soybean (Glycine max), lupin (Lupinus albus), flaxseed (Linum usitatissimum), pumpkinseed (Lepomis gibbosus), Basil (Ocimum basilicum L), Peppermint (Mentha piperita) and Marjoram (Origanum majorana L), yerba mate (Ilex paraguariensis), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), and sage (Salvia officinal) extract agar. In all of these media, over 80% of C. dubliniensis isolates showed rough colonies with peripheral hyphae fringes and abundant chlamydospores after 24 to 48 hours of incubation at 25 °C. In contrast, under the same conditions, all isolates of C. albicans (100%) showed smooth colonies without hyphae fringes or chlamydospores. In conclusion, this media subtracts offer a simple, rapid, and inexpensive screening media for the differentiation of C. albicans and C. dubliniensis.

Study the Antioxidant Activity of Sage (Salvia Officinalis) Leaves Extract

This study was carried out to evaluate two type of sage (salvia officinalis) leaves extracts (Ethanolic and Water extracts) by using two methods (reducing power and chelating ability). So this study shows the high content of phenolic compound of ethanolic and water extract of sage (15.8 and 14.6) % respectively. The leaves of sag were locally obtained, twenty gram of ground material was extracted by 250 ml ethanol 95% or distilled water at boiling point, under reflux for one hr. The extractive was filtered and evaporated at 50 C° to the compete dryness. Total phenol assay, total flavonoid assay, reducing power assay, and chelating ability assay were observed in this study. Results revealed that the ethanoic extract had highest percentage of reducing power than water extract of sage leaves as so as the chelating ability percentage.