Effects Of Nickel Oxide Nanoparticles Research Articles

Pathological Alterations Induced by Nickel Oxide Nanoparticles in Pregnant Mice and Their Embryos

The purpose of this study was to investigate the effects of nickel oxide nanoparticles on pregnant mice and fetal development. Nickel oxide nanoparticles (<50 nm) were injected intraperitoneally (1, 10, 20 mg/kg) in pregnant mice daily from the 7th -18th gestation day. At the 18th gestation day, the mice were euthanized, necropsied, and pathological examination of the mice and their embryos was done. Exposure to the nickel nanoparticles induced an increase (low and medium doses) and a decrease (high dose) in the activity of the mice. Abortion was recorded at the rates 10%, 20%, and 30% in groups treated with doses of 1, 10, and 20 mg/kg, respectively. Fetal malformations were noted at rates of 60%, 70%, and 75%, for the doses 1, 10, and 20 mg/kg, respectively. They included arrested growth, absence of the extremities, C-shaped fetuses, anencephalon, myelocele, exophthalmia, spina bifida, absence of facial details, absence or deformities of the tail, micromelia, cyanotic skin, abdominal hemorrhages, and meningocele. Microscopically, degenerative, necrotic, circulatory, and inflammatory lesions in the kidneys, liver, lungs, brain, and heart were noted in pregnant mice. In the embryos, there was incomplete growth of the renal, nervous, and pulmonary tissues. Additionally, there were degenerative, necrotic, and circulatory changes in the kidneys, liver, brain, lungs and heart. It was concluded that exposure to intraperitoneally administered nickel oxide nanoparticles (<50 nm) induces abortion and toxic lesions in the kidneys, liver, brain, lungs and heart of pregnant mice; and fetal malformations and pathological lesions in the embryos.

Effect of Nickel Oxide Nanoparticles on Antioxidant Enzyme System and Hematology of Tilapia

Effect of Nickel Oxide Nanoparticles on Antioxidant Enzyme System and Hematology of Tilapia

Effect of nickel oxide nanoparticles on bioethanol production by Pichia kudriavzveii IFM 53048 using banana peel waste substrate

ABSTRACT The use of nanomaterials in bioethanol production is promising and on the increase. In this report, the effect of nickel oxide nanoparticles (NiO NPs) on bioethanol production in the presence of a novel yeast strain, Pichia kudriavzveii IFM 53048 isolated from banana wastes was investigated. The hot percolation method was employed for the green synthesis of NiO NPs. The logistic and modified Gompertz kinetic models employed in this study showed a 0.99 coefficient of determination (R2) on cell growth, and substrate utilization on the initial rate data plot which indicate that these model were best suited for bioethanol production studies. As a result, 99.95% of the substrate was utilized to give 0.23 g/L/h−1 bioethanol productivity, and 51.28% fermentation efficiency, respectively. At 0.01 wt% of NiO NPs, maximum production was achieved with 0.27 g/g bioethanol yield. Meanwhile, 0.78 h−1 maximum specific growth rate (µmax) of the microorganism, 3.77 g/L bioethanol concentration (Pm), 0.49 g/L/h production rate (rp.m), and 2.43 h production lag time (tL) were obtained when 0.01 wt% of NiO NPs were used during the bioethanol production process. However, a decrease in bioethanol concentrations occurred at ≥0.02 wt% of NiO NPs. The incorporation of NiO NPs in the simultaneous saccharification and fermentation (SSF) process improved the production of bioethanol by 1.90 fold using banana peel wastes as substrate. These revealed NiO NPs could serve as a suitable biocatalyst in the green production of bioethanol from banana peel waste materials.

Exposure to Nickel Oxide Nanoparticles Induces Alterations in Antioxidant System, Metabolic Enzymes and Nutritional Composition in Muscles of Heteropneustes fossilis.

The current study was performed to explore potential toxic effect of nickel oxide nanoparticles (NiO NPs) on muscle tissue of catfish, Heteropneustes fossilis. Fishes were exposed to different concentrations of NiO NPs (12mg/L, 24mg/L, 36mg/L and 48mg/L) for a period of 14 days. Results revealed that NiO NPs caused significant increase in Ni accumulation, metallothionein content, lipid peroxidation and activity of different antioxidant enzymes (catalase, glutathione s transferase and glutathione reductase) while decrease in activity of superoxide dismutase (p < 0.05). Data also reported induction of Na+/K+ ATPase activity initially and then its decrease in concentration dependent manner. Fourier transform infrared spectroscopy revealed shift and changes in spectra of muscle of NiO NPs treated fishes. Fluctuations in activity of aspartate amino transferase, alanine amino transferase and alkaline phosphatase were also noticed. Nutritional contents like protein, lipid, and moisture significantly reduced while glucose and ash percent increased.

Biosynthesis of Nio Nanoparticles Using Prodigiosin Pigment and its Evaluate of Antibacterial Activity Against Biofilm Producing MDR- Pseudomonas Aeruginosa

The entire investigation's focus was on the production of nickel oxide nanoparticles (NiONPs), using prodigiosin pigments produced by Serratia marcescens as a stabilizing and reducing agent. Nickel oxide nanoparticles are synthesized using nickel sulfate NiSO4 (10mg) with a concentration of prodigiosin (10g/100ml). Biosynthesized NiO nanoparticles have been characterized by using many techniques, such as (UV-Vis, AFM, XRD, FTIR, and FE-SEM). The AFM analysis revealed that NiONPs have an average diameter size of (41.77 mm), and the FE-SEM Image displays Spherical. Additionally, the effect of NiONPs with different concentrations on the bacteria Pseudomonas aeruginosa was measured and the inhibition zone reached to (29 mm). Biosynthesis of NiONPs using prodigiosin was shown to have promising activity as an antibacterial against the biofilm-producing Pseudomonas aeruginosa.

Biogenic synthesis of nickel oxide nanoparticles using Averrhoa bilimbi and investigation of its antibacterial, antidiabetic and cytotoxic properties

• Nickel Oxide Nanoparticles were synthesized using Averrhoa bilimbi extract. • Antibacterial activity tested for two Gram positive and two Gram negative bacteria. • The nanoparticles exhibit good antidiabetic effect on α-amylase enzyme. • The material shows excellent anticancer activity against HCT-116. Numerous methods have been implemented to prepare nickel oxide nanoparticles many of which involve harmful chemicals that can be avoided by using plant or fruit extracts. In the present work, we have prepared nickel oxide nanoparticles using extract of Averrhoa bilimbi fruit. The bioactive compounds like tannins, flavonoids, and phenols present in the fruits act as a reducing and capping agent to convert nickel nitrate to nickel oxide nanoparticles. The prepared nanoparticles are subjected to structural, optical, and morphological analysis by Powder X-ray Diffraction pattern (P-XRD), Fourier Transform-Infrared Spectroscopy (FTIR), UV–visible analysis (UV–vis), Scanning Electron Microscopic technique (SEM), and EDAX analysis. The magnetic property of the prepared nanoparticles is examined by Vibrating Sample Magnetometer (VSM). Nickel Oxide nanoparticles exhibit potent antibacterial activity against E.coli and S.auerus with a higher region of zone of inhibition at 150 µgmL −1 . The antidiabetic effect of the prepared sample is investigated and found to exhibit their inhibitory effect on α-amylase enzyme with IC 50 value 311.26 µgmL −1 ( p < 0.005). Finally, the cytotoxic effects of nickel oxide nanoparticles are studied in cultured human colorectal cancer cells (HCT-116), which have exhibited significant anticancer activity with 55 µgmL −1 at 50 % inhibition concentration (IC 50 ). From the results, nickel oxide nanoparticles may offer a safe potential for diabetes and cancer management and can be applied to different medical and industrial applications.

Impact of Nickel Oxide Nanoparticles (NiO) on Oxidative Stress Biomarkers and Hemocyte Counts of Mytilus galloprovincialis.

In this study, the toxic effects of nickel oxide nanoparticles (NiO-NPs) on the model organism Mediterranean mussel (Mytilus galloprovincialis) gill, digestive gland, and hemolymph tissues for 96h were investigated. Lipid peroxidation (MDA) determination was performed to reveal the oxidative stress generation potential of nanoparticles, and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) enzyme levels were measured to determine antioxidant responses. Lysosomal membrane stability and total hemocyte counts were performed to determine cytotoxic effects. All parameters were altered in different concentrations of NiO-NPs (2, 20, and 200mg L-1). The SOD levels increased depending on the concentration (p < 0.05), and the increases in CAT, GPx, and GST levels were lower at 20mg L-1 concentration (p < 0.05). There was a slight difference between the exposure and the control groups in terms of GR enzyme. The MDA level increased in parallel with the concentration (p < 0.05), the stability of the cell membrane (p < 0.05), and the number of hemocyte cells decreased as a result of exposure (p < 0.05). The results emphasize that NiO-NPs may have negative effects on the aquatic environment.

Effect of nickel oxide nanoparticles on protease activity in brewing industry

Intensive use of nanoparticles on an industrial scale leads to an increase in their content in the environment. This increases the risks of nano-sized objects entering the technological chains of the processing industries of the agro-industrial complex, in particular, brewing. The paper presents the results of the studies of the effect of nickel oxide nanoparticles used in various industries on the activity of the proteolytic type of enzyme preparation Neutrase 1.5MG, as well as on the results of laboratory mashing of light barley malt. The effect of different concentrations of NiO nanoparticles on the accumulation of low molecular weight nitrogenous substances during gelatin hydrolysis in model media was determined. It was found that if the content of nanoparticles exceeds 0.25 mg/cm3, the proteolytic capacity of the enzyme preparation is reduced up to 70% compared to the control at the concentration of nickel oxide nanopreparation of 2.0 mg/cm3. The experiments showed that an increase in the duration of contact between nanoparticles and proteases of the enzyme preparation in the reaction medium did not lead to an increase in the inhibitory effect of the nano-sized NiO. The laboratory mashing revealed more pronounced negative effect of nickel oxide nanoparticles on the accumulation of low-molecular nitrogen compounds. It is determined that in the presence of NiO nanopreparation, the hydrolysis efficiency of the starchy components of light barley malt is reduced. As a result, according to a number of indicators (concentration of amine nitrogen, reducing substances), the first wort obtained by mashing in the presence of nickel oxide nanoparticles at both lower (0.25 mg/cm3) and higher (2.0 mg/cm3) concentrations is inferior to samples obtained in the absence of nano-sized particles. Based on the above data, it is concluded that the presence of NiO nanoparticles in brewing environments is undesirable.

Biochemical and histological alterations induced by nickel oxide nanoparticles in the ground beetle Blaps polychresta (Forskl, 1775) (Coleoptera: Tenebrionidae).

The present study evaluates the effect of nickel oxide nanoparticles on some biochemical parameters and midgut tissues in the ground beetle Blaps polychresta as an indicator organism for nanotoxicity. Serial doses of the NiO-NPs colloid (0.01, 0.02, 0.03, 0.04, 0.05, and 0.06 mg/g) were prepared for injecting into the adult beetles. Insect survival was reported daily for 30 days, and the sublethal dose of 0.02 mg/g NiO-NPs was selected for the tested parameters. After the treatment, nickel was detected in the midgut tissues by X-ray microanalysis. The treated group demonstrated a significant increase in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities when compared to the untreated group. However, the treated group demonstrated a significant decrease in ascorbate peroxidase (APOX) activity when compared to the untreated group. Histological and ultrastructural changes in the midgut tissues of treated and untreated beetles were also observed. The current findings provide a precedent for describing the physiological and histological changes caused by NiO-NPs in the ground beetle B. polychresta.

Effects of nickel oxide nanoparticles on survival, reproduction, and oxidative stress biomarkers in the marine calanoid copepod Centropages ponticus under short-term exposure.

Excessive use of nickel oxide nanoparticles (NiO NPs) in various industrial and commercial products can lead to various negative effects in human and environmental health due to their possible discharge into the environment. Nerveless, information about their ecotoxicological effects on marine organisms are lacking. Copepods are good ecotoxicological models because of their high sensitivity to environmental stress and their key role in the marine food webs. In this study, 48h acute tests were conducted on the marine planktonic copepod Centropages ponticus to assess lethal and sublethal toxicities of NiO NPs. The results revealed LC50 (48h) of 4mg/L for adult females. Aggregation and settling of NiO NPs were observed at concentrations ≥ 2mg/L. Exposure to sublethal concentrations (≥ 0.02mg/L for 48h) had significant negative effects on reproductive success in C. ponticus. Egg production after 24h and 48h decreased by 32% and 46%, respectively at 0.02mg/L and 70% and 82%, respectively, at 2mg/L. Hatching success was reduced by 70% and 79% at 2mg/L for eggs produced after 24h and 48h respectively. Antioxidant enzymatic activity increased significantly with NiO NP concentration and time, indicating that NiO NPs can cause oxidative stress in C. ponticus even under short-term exposure, while significant inhibition of acetylcholinesterase activity at 2mg/L after 48h suggests neurotoxic effects of NiO NPs.

In Vitro Evaluation of Cytotoxic Effects of Nickel Oxide Nanoparticles on Human Neuroblastoma Cell Line (SH-SY5Y)

In Vitro Evaluation of Cytotoxic Effects of Nickel Oxide Nanoparticles on Human Neuroblastoma Cell Line (SH-SY5Y)

Exposure to variable doses of nickel oxide nanoparticles disturbs serum biochemical parameters and oxidative stress biomarkers from vital organs of albino mice in a sex-specific manner

Introduction This study was designed to report the biological effect of nickel oxide nanoparticles (NiO NPs) in albino mice. Material and methods Five weeks old albino mice of both sex were intraperitoneally injected either with 20 mg (low dose) or 50 mg/mL saline/kg body weight (high dose) of NiO NPs for 14 days. Saline-treated controls were maintained in parallel. Complete blood count, selected serum biochemical parameters and oxidative stress biomarkers from vital organs were determined in all subjects. Results Male mice treated with NiO NPS had increased blood urea nitrogen, elevated superoxide dismutase (SOD) in liver elevated MDA in liver, kidney and heart and reduced catalase activity in heart and kidney. Female mice treated with NiO NPs had significantly reduced serum albumin and total proteins, SOD in lungs and elevated MDA in liver. Discussion We are reporting that intraperitoneal injections of NiO NPs for 14 days drastically affect blood serum parameters and oxidative stress biomarkers from vital organs of albino mice. Conclusion Toxic effects of NiO NPs were dose and sex dependent and they were more pronounced at higher dose and in male mice.

Cytotoxic, Genotoxic, and Apoptotic Effects of Nickel Oxide Nanoparticles in Intestinal Epithelial Cells.

The superior properties of nickel oxide-nanoparticles (NiO-NPs) have led to their wide use in various fields. However, there is little comprehensive knowledge about their toxicity, especially after oral exposure. The toxic effect of NiO-NPs of mean size 15.0 nm was investigated in Caco-2 (human intestinal epithelial) cells as no study has been performed on their intestinal toxicity. Following identification of their particle size distribution and cellular uptake potential, the risk of exposure to NiO-NPs was evaluated by cellular morphologic changes, cyto- and genotoxic potentials, oxidative damage, and apoptotic induction. NiO-NPs induced a 50% reduction in cell viability at 351.6 μg/mL and caused DNA damage and oxidative damage at 30-150 μg/mL. It appears that apoptosis might be a main cell death mechanism in NiO-NP-exposed intestinal cells. NiO-NPs might be hazardous to the gastrointestinal system. The results should raise concerns about using NiO-NPs in food-contact appliances and about NiO-NP-containing wastes. Further in vivo and in vitro research should be conducted to explain the specific toxicity mechanism of these particles and reduce their risk to humans.

Histological Study of Pregnant albino Rat Treated with Nickel Oxide Nanoparticles

The present study was aimed to elucidate the effect of Nickel oxide nanoparticles (100 mg / kg b.wt ) on some parameters in pregnant Albino rats,in both period of pregnancy (12 and14 days ). 30 albino rats were used; 20 female and the rest 10 were fertilized male rats which were used insemination only, the study including, maternal relative body weight, weight gain measurements and relative organ weights and histological study. Relative body weight of mothers showed decreases in both period of pregnancy. Body weight gain of the pregnant rat show decreases in 14 days of pregnancy in treated group as compared with control group while in 12 days of pregnancy showed increases in treated group as compared with control. Relative organ weight showed increases in (Lung, Uterus, kidney) in treated group and decreases in (Heart, Liver, Eye, Spleen, Brian) in (12) day of pregnancy. In ( 14 ) day of pregnancy showed decreases in (Heart, Liver, Eye, Brian , Kidney) and increases in (Lung, Spleen, Uterus) in treated group as compared with control group Histological study included some organ (liver, spleen, kidney, lung brain, eye and heart) for different period (12, 14) days of pregnancy.

Effect of nickel oxide nanoparticles on bioethanol production: Process optimization, kinetic and metabolic studies

The present study optimized ethanol yield using nickel oxide (NiO) nanoparticles (NPs) as a biocatalyst. Additionally, Saccharomyces cerevisiae BY4743 cell growth and the bioethanol production kinetics were assessed. The Response Surface Methodology (RSM) model showed a coefficient of determination (R2) value of 0.93. The optimized process gave a biomass concentration and ethanol yield of 2.04 g/L and 0.26 g/g (1.03 and 1.19-fold increment compared to the control experiment), respectively. The process kinetic data showed that the inclusion of NiO NPs improved the affinity of S. cerevisiae BY4743 to glucose consumption, carbohydrate and protein accumulation. A significant reduction in volatile fatty acid (VFA) was observed in the presence of NiO NPs. The application of nano biocatalyst in simultaneous saccharification and fermentation of potato peel waste, meaningfully enhanced bioethanol production (>65 %). The study provided major insights into the use of NiO NPs to enhance the bioprocess of ethanol production.

The effects of nickel oxide nanoparticles on structural changes, heme degradation, aggregation of hemoglobin and expression of apoptotic genes in lymphocytes

Nickel oxide nanoparticles (NiO NPs) have received great interests in medical and biotechnological applications. However, their adverse impacts against biological systems have not been well-explored. Herein, the influence of NiO NPs on structural changes, heme degradation and aggregation of hemoglobin (Hb) was evaluated by UV-visible (Vis) spectroscopy, circular dichroism (CD) spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM), and molecular modeling investigations. Also, the morphological changes and expression of Bax/Bcl-2 mRNA in human lymphocyte cell exposed to NiO NPs were assayed by DAPI staining and quantitative real-time PCR (qPCR), respectively. The UV-Vis study depicted that NiO NPs resulted in the displacement of aromatic residues and heme groups and production of the pro-aggregatory species. Intrinsic and Thioflavin T (ThT) fluorescence studies revealed that NiO NPs resulted in heme degradation and amorphous aggregation of Hb, respectively, which the latter result was also confirmed by TEM study. Moreover, far UV-CD study depicted that NiO NPs lead to substantial secondary structural changes of Hb. Furthermore, near UV-CD displayed that NiO NPs cause quaternary conformational changes of Hb as well as heme displacement. Molecular modelling study also approved that NiO NPs resulted in structural alterations of Hb and heme deformation. Moreover, morphological and genotoxicity assays revealed that the DNA fragmentation and expression ratio of Bax/Bcl-2 mRNA increased in lymphocyte cells treated with NiO NPs for 24 hr. In conclusion, this study indicates that NiO NPs may affect the biological media and their applications should be limited.Communicated by Ramaswamy H. Sarma

Bio-based synthesized NiO nanoparticles and evaluation of their cellular toxicity and wastewater treatment effects

In this study, nickel oxide nanoparticles have been synthesized through the utilization of a sol-gel approach that contained various temperatures. Chitosan has been appointed to perform polymerization and function as an endpoint agent throughout this project to grow the particles, as well as carrying out the procedures of preventing agglutination, increasing stability, and reducing toxicity, respectively. Phase study, morphology, and determining the structure of nickel oxide nanoparticles have been evaluated by the means of FTIR, XRD, UV-VIS, TGA/DTA, FESEM/EDX, and VSM analyzes. In addition, the photocatalytic effects of nickel oxide nanoparticles on methylene blue (MB) degradation have been investigated and in accordance with the obtained results, they contain great potential as photocatalysts under UV light. The XRD studies have confirmed that the nanoparticles seem to accommodate a face-centered cubic (fcc) crystalline structure and a space group (Fm3m). The range of phase-formation temperature of NiO-NPs has been obtained from the TGA/DTA analyses. The VSM examination has shown the superparamagnetism quality of the sample that had been synthesized at 500 °C. To further analyze the safety of NiO-NPs, the evaluation of their biocompatibility on human cancer U87MG cell lines has been performed by the means of MTT assay, which has indicated cell toxicity at concentrations up to 8 μg/mL.

The effects of nickel oxide nanoparticles on tau protein and neuron-like cells: Biothermodynamics and molecular studies

Herein, the thermodynamic parameters of tau upon interaction with NiO NPs were determined by fluorescence spectroscopy. Also, molecular docking studies were run to explore the binding affinities of NiO NPs clusters with different sizes of 30 Å and 50 Å toward tau. Also, cytotoxic activity of NiO NPs against SH-SY5Y was determined by MTT, LDH, caspase-9/3 activity, and expression of apoptotic Bax and Bcl-2 genes assays.DLS study showed that NiO solution had a good colloidal stability. Fluorescence study revealed that KSV values were 2.95 ± 0.35 × 104, 3.31 ± 0.59 × 104 and 3.92 ± 0.65 × 104 at 298 K, 310 K and 315 K, respectively. Also, ∆G° (kJ/mol), ∆H° (kJ/mol) and T∆S° (kJ/mol) values were − 13.27 ± 1.57, 1.98 ± 0.14, 15.25 ± 2.01, respectively at 298 K. Theoretical studies depicted that affinity of 5O3T segment toward NiO NP (30 Å) is higher than NiO NP (50 Å) and the proportion of Lys residues are higher in the docked pose of NiO NP (30 Å)/5O3T complex than NP (50 Å)/5O3T complex. Moreover, NiO NPs demonstrated a significant increase in the mortality of SH-SY5Y cells in an apoptotic manner. This study determined that NiO NPs may mediate the formation of electrostatic interactions with tau and induction of undesired harmful effects on neurons.

Activity and stability of lysozyme obtained from Rutilus frisii kutum in the presence of nickel oxide nanoparticles

The aim of this study was to examine the effects of nickel oxide nanoparticles on the activity and thermal stability of lysozyme obtained from Rutilus frisii kutum. The synthesis of the nanoparticles was performed by the chemical co-precipitation method. To evaluate the synthesis process, X-ray diffraction, scanning electron microscope, and Fourier transform infrared spectroscopy were evaluated. The size of the nanoparticles was in the range of 20–30 nm with a spherical morphology and gray–black color. To determine thermal stability, the fraction unfolded curve and ΔG° as a function of lysozyme temperature in the absence and presence of the nanoparticles were obtained. With respect to Tm at ΔG° = 0, we obtained Tm of lysozyme from 314 K in the absence of the nanoparticles to 332 K in the presence of 0.0001 g/cm3 nickel oxide nanoparticles. Therefore, thermal stability of lysozyme was increased with the rise of nanoparticles’ concentrations. The reversible unfolding as a result of heating lysozyme was observed at Tm = 318 K. Kinetic parameters, Km and Vmax, of the enzyme were also determined from 0.007 g/cm3 and 172 units/min in the absence of the nanoparticles to 0.005 g/cm3 and 104.166 units/min in the presence of 0.0001 g/cm3 nickel oxide nanoparticles, respectively. The emission intensity of lysozyme decreased in the presence of the nanoparticles, while its absorption intensity increased without any shift at 340 and 280 nm, respectively. Consequently, the nickel oxide nanoparticles can interact with lysozyme that results in decreasing its enzymatic activity due to changes in its active site.

Dynamic Mechanical Properties of Effect of Nickel Oxide Nanoparticles in Polyester based Nanocomposites.

Dynamic Mechanical Properties of Effect of Nickel Oxide Nanoparticles in Polyester based Nanocomposites.