Effects of nickel oxide nanoparticles on survival, reproduction, and oxidative stress biomarkers in the marine calanoid copepod Centropages ponticus under short-term exposure.

Abstract

Excessive use of nickel oxide nanoparticles (NiO NPs) in various industrial and commercial products can lead to various negative effects in human and environmental health due to their possible discharge into the environment. Nerveless, information about their ecotoxicological effects on marine organisms are lacking. Copepods are good ecotoxicological models because of their high sensitivity to environmental stress and their key role in the marine food webs. In this study, 48h acute tests were conducted on the marine planktonic copepod Centropages ponticus to assess lethal and sublethal toxicities of NiO NPs. The results revealed LC50 (48h) of 4mg/L for adult females. Aggregation and settling of NiO NPs were observed at concentrations ≥ 2mg/L. Exposure to sublethal concentrations (≥ 0.02mg/L for 48h) had significant negative effects on reproductive success in C. ponticus. Egg production after 24h and 48h decreased by 32% and 46%, respectively at 0.02mg/L and 70% and 82%, respectively, at 2mg/L. Hatching success was reduced by 70% and 79% at 2mg/L for eggs produced after 24h and 48h respectively. Antioxidant enzymatic activity increased significantly with NiO NP concentration and time, indicating that NiO NPs can cause oxidative stress in C. ponticus even under short-term exposure, while significant inhibition of acetylcholinesterase activity at 2mg/L after 48h suggests neurotoxic effects of NiO NPs.