Exposure to Nickel Oxide Nanoparticles Induces Alterations in Antioxidant System, Metabolic Enzymes and Nutritional Composition in Muscles of Heteropneustes fossilis.

Abstract

The current study was performed to explore potential toxic effect of nickel oxide nanoparticles (NiO NPs) on muscle tissue of catfish, Heteropneustes fossilis. Fishes were exposed to different concentrations of NiO NPs (12mg/L, 24mg/L, 36mg/L and 48mg/L) for a period of 14 days. Results revealed that NiO NPs caused significant increase in Ni accumulation, metallothionein content, lipid peroxidation and activity of different antioxidant enzymes (catalase, glutathione s transferase and glutathione reductase) while decrease in activity of superoxide dismutase (p < 0.05). Data also reported induction of Na+/K+ ATPase activity initially and then its decrease in concentration dependent manner. Fourier transform infrared spectroscopy revealed shift and changes in spectra of muscle of NiO NPs treated fishes. Fluctuations in activity of aspartate amino transferase, alanine amino transferase and alkaline phosphatase were also noticed. Nutritional contents like protein, lipid, and moisture significantly reduced while glucose and ash percent increased.