Disease In Domestic Animals Research Articles

First report of human Thelazia callipaeda infection in Portugal

The zoonotic parasitic nematode Thelazia callipaeda, also known as the oriental eye worm, is endemic in several European countries, including Portugal. Infections may result in ocular disease in domestic and wild animals as well as humans, with more or less severe manifestations. We report the first human case of ocular thelaziosis by T. callipaeda in Portugal, a country where the parasite had already been found to infect dogs, cats, red foxes, wild rabbits and a beech marten. An 80-year-old patient from east-central Portugal, who had been suffering from tearing for a few years, had whitish filiform fragments removed from the left eye. Polymerase chain reaction of partial cytochrome c oxidase subunit 1 and 18S small subunit rRNA genes followed by bidirectional sequencing and BLAST analysis confirmed T. callipaeda haplotype 1, the only haplotype previously reported in Europe. The endemicity of T. callipaeda in domestic and wild animals in east-central Portugal makes it very likely that infection of the human patient had occurred locally. In east-central and other geographical areas of Portugal, veterinarians and physicians, especially ophthalmologists, should regard T. callipaeda as a cause of ocular pathology in animals and humans.

CLINICAL AND MORPHOLOGICAL SUBSTANTIATION OF INTRANASAL LYMPHOMAS OF CATS

The article discusses results of the research on intranasal lymphoma spread of cats, based on the study of 38 clinical cases of animals with lesions of the nasal cavity at the age of 5 months to 18 years with clinical symptoms from the total number (N=4937) of cats. The research was conducted at the Department of Diseases of Small Domestic, Laboratory and Exotic animals of FSBEI HE "Moscow State University of Food Production" and the veterinary clinic "Zoogalereya", Moscow. Intranasal neoplasms are rare for cats. Of all nasal oncopathies, lymphomas are most common in animals of the middle age group and, according to the literature data, take up to 1% of all tumors of cats. Endogenous and exogenous factors that cause tumors of cats’ nasal cavity are diverse. If intranasal pathology is suspected, it is necessary to adhere to the plan of diagnostic manipulations, because the first clinical symptoms of the process are the same for lesions of a different nature. For diagnosis and specification of the clinical stage of the disease, it is necessary to conduct a series of diagnostic studies, including radiography, MRI and CT, aimed at determining the localization of the pathology, spread, degree of invasion and degree of involvement of blood vessels in a specific oncological process. Such diagnostic methods as palpation of regional lymph nodes, chest radiographs and ultrasonography of the abdominal organs are aimed at determining the clinical stage of oncopathologies. The most predisposed to occurrence of intranasal lymphomas was a group of cats aged 5-9 years. Gender and breed susceptibility was not revealed. In case of nasal cavity pathologies of cats, a common symptom is shortness of breath, lack of patency of nasal passages, which appears at later stages of the disease. Nasal discharge and epistaxis are rare, convulsive seizures - when the tumor invades via the ethmoid bone. The predominance of unilateral over bilateral discharge was noted as the most common symptom, 39.4%, while the nature of the discharge may be different. Sneezing occurred in 13.2%, coughing in 5.2% of cases. Deformation of the facial part of the skull was observed in 28.9% of cats, epistaxis, lacrimation and atony - in 13.2% of cases, 5.2% of cats had neurological symptoms.

Integrative Alternative Tactics for Ixodid Control.

Simple SummaryHard ticks are important for economic and health reasons, and control has mainly relied upon use of synthetic acaricides. Contemporary development of resistance and concerns relating to health and environmental safety have elicited exploration into alternative tactics for hard tick management. Some examples of alternative tactics involve biological control, desiccant dusts, growth regulators, vaccines, cultural methods, and ingested medications.Ixodids (hard ticks), ectoparasitic arthropods that vector the causal agents of many serious diseases of humans, domestic animals, and wildlife, have become increasingly difficult to control because of the development of resistance against commonly applied synthetic chemical-based acaricides. Resistance has prompted searches for alternative, nonconventional control tactics that can be used as part of integrated ixodid management strategies and for mitigating resistance to conventional acaricides. The quest for alternative control tactics has involved research on various techniques, each influenced by many factors, that have achieved different degrees of success. Alternative approaches include cultural practices, ingested and injected medications, biological control, animal- and plant-based substances, growth regulators, and inert desiccant dusts. Research on biological control of ixodids has mainly focused on predators, parasitoid wasps, infective nematodes, and pathogenic bacteria and fungi. Studies on animal-based substances have been relatively limited, but research on botanicals has been extensive, including whole plant, extract, and essential oil effects on ixodid mortality, behavior, and reproduction. The inert dusts kaolin, silica gel, perlite, and diatomaceous earth are lethal to ixodids, and they are impervious to environmental degradation, unlike chemical-based toxins, remaining effective until physically removed.

Seroepidemiology of Camel Brucellosis in and around Dire Dawa, Eastern Ethiopia.

Brucellosis is an infectious disease in domestic and wild animals with serious zoonotic and economic implication in humans, being more severe in developing countries. The disease is highly prevalent in cattle, camels, and small ruminants in pastoral and agro-pastoral areas in Africa. Here we have investigated the seroepidemiology of camel brucellosis in and around Dire Dawa, eastern Ethiopia, using a cross-sectional study design to determine the seroprevalence of the disease and to identify risk factors that would facilitate the transmission of zoonotic diseases to humans. This study involved testing 350 serum samples from camels and interviewing 120 livestock owners. The modified Rose Bengal plate test (mRBPT) and the complement fixation test (CFT) were used as screening and confirmatory tests, respectively. The overall sero-prevalence of camel brucellosis was found to be 8.3% and 2% using mRBPT and CFT tests, respectively. Among the risk factors assessed, only abortion and body condition disclosed a statistically significant difference (p < 0.05) with regard to the seropositivity of camel brucellosis. Camel brucellosis is prevalent in eastern Ethiopia and there is a need to execute well-organized disease control and prevention programs and exercise public health education to scale up awareness of the community towards the disease.

RNA Virus Diversity in Birds and Small Mammals From Qinghai–Tibet Plateau of China

Most emerging and re-emerging viruses causing infectious diseases in humans and domestic animals have originated from wildlife. However, current knowledge of the spectrum of RNA viruses in the Qinghai-Tibet Plateau in China is still limited. Here, we performed metatranscriptomic sequencing on fecal samples from 56 birds and 91 small mammals in Tibet and Qinghai Provinces, China, to delineate their viromes and focused on vertebrate RNA viruses. A total of 184 nearly complete genome RNA viruses belonging to 28 families were identified. Among these, 173 new viruses shared <90% amino acid identity with previously known viral sequences. Several of these viruses, such as those belonging to genera Orthonairovirus and Hepatovirus, could be zoonotic viruses. In addition, host taxonomy and geographical location of these viruses showed new hosts and distribution of several previously discovered viruses. Moreover, 12 invertebrate RNA viruses were identified with <40% amino acid identity to known viruses, indicating that they belong to potentially new taxa. The detection and characterization of RNA viruses from wildlife will broaden our knowledge of virus biodiversity and possible viral diseases in the Qinghai–Tibet Plateau.

Function of lipid binding proteins of parasitic helminths: still a long road.

Infections with parasitic helminths cause severe debilitating and sometimes lethal diseases in humans and domestic animals on a global scale. Unable to synthesize de novo their own fatty acids and sterols, helminth parasites (nematodes, trematodes, cestodes) rely on their hosts for their supply. These organisms produce and secrete a wide range of lipid binding proteins that are, in most cases, structurally different from the ones found in their hosts, placing them as possible novel therapeutic targets. In this sense, a lot of effort has been made towards the structure determination of these proteins, but their precise function is still unknown. In this review, we aim to present the current knowledge on the functions of LBPs present in parasitic helminths as well as novel members of this highly heterogeneous group of proteins.

Epizootic situation with invasive diseases in Kursk region

The article analyzes the data for five years, from 2017 to 2021, the most common invasive diseases of agricultural and domestic animals. It was noted that in cattle, the extensiveness of infestation for fasciolosis decreased from 4.5% to only 0.5%, for dictyocaulosis from 2.9% to 0.2%, while strongylatosis became more frequent by 0.7% and trichocephalus by 1.2%. It was found that in small ru-minants dictyocausalosis occurred less frequently than in cattle. The rate of inva-sion by fasciolosis was decreased by 4%, by strongylosis - by 0,1%, the rate of trichocephalosis decreased from 1,9% to 0,2%. The incidence of ascariasis among pigs decreased from 0.55% to 0.2%, esophagostomosis from 0.2% to 0.1%. Among horses a marked decrease in the extensiveness of invasion by parascarido-sis from 8.7% to 8.3% is noted. The extensiveness of invasion of dogs has been determined as for dirofilariosis during five years it decreased from 3% to 0.9%, for toxocariasis and trichocephalosis from 61.7% to 15.7% and for pyroplasmosis it became more frequent by 26.1%. The region has established close work between the veterinary service and scientists of the Department of Veterinary Medicine, and courses are held annually to im-prove the skills of livestock workers. A system for monitoring and prophylaxis of carnivore dyrofilariosis has been developed jointly. The system offers routine, tar-geted monitoring of canine blood tests for dirofilariasis in the amount of at least 200 samples per year per subject. On a monthly basis, animal disease control sta-tions and inter-district veterinary laboratories submit information to the rehabilita-tion unit on the number of dogs inspected and found to be infected, with the names and addresses of their owners. The results are used to eliminate the source of infes-tation by microfilarial treatment of identified infested animals.

Analysis of the spread of oncological diseases of domestic unproductive animals in the administrative districts of the city of Volgograd

The article presents the results of a retrospective analysis of the spread of oncological diseases in dogs and cats, on the territory of two administrative districts of the city of Volgograd. The object of the study was 127 dogs and 117 cats, differing in gender, age and breed, who were admitted to the clinic with neoplasms of various origin and localization. The most common oncological pathologies in dogs and cats in the Traktorozavodsky and Krasnooktyabrsky districts of the city have been identified. The dogs were diagnosed with tumors of the breast - 33.8%, skin and its derivatives - 29% and the reproductive system - 11.8%. At the same time, breast neoplasms were detected in cats – 62.4%, skin - 9.4%, oral cavity - 8.5% and hematopoietic system - 8.5%.

Molecular Detection of Avipoxvirus in Wild Birds in Central Italy.

Simple SummaryAvipoxviruses (APVs) are responsible for diseases in domestic and wild birds. Currently, the disease in domestic animals is under control in many Countries by biosafety and vaccination. In wild birds, small disease events are frequently reported worldwide, but large outbreaks are generally rare. Nevertheless, some aspects of the epidemiology of these viruses are still unclear. In this study, we explored, through molecular investigations, the diffusion of APVs among wild birds, of different orders and species, without typical macroscopic lesions. A high percentage (43.33%) of positive specimens was detected, suggesting high diffusion of the viruses and a possible role of avian wildlife as a reservoir. Aquatic birds, mainly Anseriformes, were more often infected, probably in relation to the environment where they live; in fact, APVs are frequently transmitted by mosquitos, particularly abundant in humid areas.Avipoxviruses (APVs) are important pathogens of both domestic and wild birds. The associated disease is characterized by skin proliferative lesions in the cutaneous form or by lesions of the first digestive and respiratory tracts in the diphtheritic form. Previous studies investigated these infections in symptomatic wild birds worldwide, including Italy, but data about the circulation of APVs in healthy avian wildlife are not available. The present study tested spleen samples from 300 wild birds without typical lesions to detect Avipoxvirus DNA. Overall, 43.33% of the samples scored positive. Aquatic birds were more frequently infected (55.42%) than other animals (26.40%), and in Anseriformes, high positivity was found (52.87%). The obtained results suggest that wild birds could be asymptomatic carriers of Avipoxviruses, opening new possible epidemiological scenarios.

Babesiosis and Theileriosis in North America.

Babesia and Theileria are apicomplexan parasites that cause established and emerging diseases in humans, domestic and wild animals. These protozoans are transmitted by Ixodid ticks causing babesiosis or theileriosis, both characterized by fever, hemolytic anemia, jaundice, and splenomegaly. In North America (NA), the most common species affecting humans is B. microti, which is distributed in the Northeastern and Upper Midwestern United States (US), where the tick vector Ixodes scapularis is established. In livestock, B. bovis and B. bigemina are the most important pathogens causing bovine babesiosis in tropical regions of Mexico. Despite efforts toward eradication of their tick vector, Rhipicephalus microplus, B. bovis and B. bigemina present a constant threat of being reintroduced into the southern US and represent a continuous concern for the US cattle industry. Occasional outbreaks of T. equi, and T. orientalis have occurred in horses and cattle, respectively, in the US, with significant economic implications for livestock including quarantine, production loss, and euthanasia of infected animals. In addition, a new species, T. haneyi, has been recently discovered in horses from the Mexico-US border. Domestic dogs are hosts to at least four species of Babesia in NA that may result in clinical disease that ranges from subclinical to acute, severe anemia. Herein we review the pathogenesis, diagnosis, and epidemiology of the most important diseases caused by Babesia and Theileria to humans, domestic and wild animals in Canada, the US, and Mexico.

Antiviral Activity of Plantago asiatica Polysaccharide against Pseudorabies Virus In Vitro.

Pseudorabies (PR) is an acute infectious disease of various domestic animals and wild animals caused by pseudorabies virus (PRV). It is mainly characterized by fever, itching, encephalomyelitis, and respiratory and neurological disorders. Plantago asiatica polysaccharide (PLP), extracted from the whole plant of Plantago asiatica L., showed immunomodulatory and antioxidation effects, but the antiviral activity had not been reported. In this study, the inhibitory effect of PLP on PRV infection was studied. Our study first revealed that PLP could inhibit PRV infection in a dose-dependent manner. By adding PLP at different stages of the virus's life cycle, we revealed that PLP could reduce the attachment and penetration of PRV into PK15 cells. The inhibition of PRV attachment was better than inhibition of PRV penetration. However, PLP did not affect PRV replication and inactivation. In addition, PLP decreased the intracellular ROS levels in infected cells significantly, and ROS scavenger NAC decreased PRV infection. Therefore, our study provided preliminary data of anti-PRV activity of PLP, which was established to be a novel anti-PRV infection agent.

The Myotis chiloensis Guano Virome: Viral Nucleic Acid Enrichments for High-Resolution Virome Elucidation and Full Alphacoronavirus Genome Assembly.

Bats are widespread mammals of the order Chiroptera. They are key for ecosystem functioning, participating in crucial processes. Their unique ability amongst mammals to fly long distances, their frequently large population sizes, and their longevity favor infectious agent persistence and spread. This includes a large variety of viruses, encompassing many important zoonotic ones that cause severe diseases in humans and domestic animals. Despite this, the understanding of the viral ecological diversity residing in bat populations remains unclear, which complicates the determination of the origins of zoonotic viruses. To gain knowledge on the viral community of a widely distributed insectivorous bat species, we characterized the guano virome of a native Chilean bat species (Myotis chiloensis (Waterhouse, 1840)). By applying a novel enrichment strategy, we were able to secure a consequent percentage of viral reads, providing unprecedented resolution for a bat virome. This in turn enabled us to identify and assemble a new bat alphacoronavirus from Chilean bats closely related to PEDV, an important viral pathogen with high mortality rates in suckling piglets. This study highlights the importance of applying and improving high-resolution virome studies in this vital order to ultimately enhance epidemiological surveillance for potentially zoonotic pathogens.

Luteolin increases susceptibility to macrolides by inhibiting MsrA efflux pump\xa0in Trueperella pyogenes

Trueperella pyogenes (T. pyogenes) is an opportunistic pathogen associated with a variety of diseases in many domestic animals. Therapeutic treatment options for T. pyogenes infections are becoming limited due to antimicrobial resistance, in which efflux pumps play an important role. This study aims to evaluate the inhibitory activity of luteolin, a natural flavonoid, on the MsrA efflux pump and investigate its mechanism. The results of antimicrobial susceptibility testing indicated that the susceptibility of msrA-positive T. pyogenes isolates to six macrolides increased after luteolin treatment, while the susceptibility of msrA-negative isolates showed no change after luteolin treatment. It is suspected that luteolin may increase the susceptibility of T. pyogenes isolates by inhibiting MsrA activity. After 1/2 MIC luteolin treatment for 36 h, the transcription level of the msrA gene and the expression level of the MsrA protein decreased by 55.0–97.7% and 36.5–71.5%, respectively. The results of an affinity test showed that the equilibrium dissociation constant (KD) of luteolin and MsrA was 6.462 × 10–5 M, and hydrogen bonding was predominant in the interaction of luteolin and MsrA. Luteolin may inhibit the ATPase activity of the MsrA protein, resulting in its lack of an energy source. The current study illustrates the effect of luteolin on MsrA in T. pyogenes isolates and provides insight into the development of luteolin as an innovative agent in combating infections caused by antimicrobial-resistant bacteria.

Northern Ireland farm-level management factors for recurrent bovine tuberculosis herd breakdowns.

Bovine tuberculosis (bTB) is a chronic, infectious and zoonotic disease of domestic and wild animals caused mainly by Mycobacterium bovis. This study investigated farm management factors associated with recurrent bTB herd breakdowns (n = 2935) disclosed in the period 23 May 2016 to 21 May 2018 and is a follow-up to our 2020 paper which looked at long duration bTB herd breakdowns. A case control study design was used to construct an explanatory set of farm-level management factors associated with recurrent bTB herd breakdowns. In Northern Ireland, a Department of Agriculture Environment and Rural Affairs (DAERA) Veterinarian investigates bTB herd breakdowns using standardised guidelines to allocate a disease source. In this study, source was strongly linked to carryover of infection, suggesting that the diagnostic tests had failed to clear herd infection during the breakdown period. Other results from this study associated with recurrent bTB herd breakdowns were herd size and type (dairy herds 43% of cases), with both these variables intrinsically linked. Other associated risk factors were time of application of slurry, badger access to silage clamps, badger setts in the locality, cattle grazing silage fields immediately post-harvest, number of parcels of land the farmer associated with bTB, number of land parcels used for grazing and region of the country.

Распространение, патогенез и комплексная терапия лептоспироза собак

Leptospirosis is a natural focal infectious disease of domestic and wild animals of many species, including humans. This disease in the conditions of the Bezhitsky veterinary hospital in Bryansk tends to increase over the past three years. In 2019, 9 dogs suffering from leptospirosis were registered, in 2020 – 12, in 2021 – 16 dogs. The change in biochemical parameters with varying degrees of severity of the course of leptospirosis was profound. The total protein content throughout the experiment was lower than the control by 29.6, 25.4 and 32.7 %, respectively. Indicators of nitrogenous products in the blood – urea and creatinine had a tendency to increase. Glucose on days 1-9-18 of the experiment was lower than in control individuals in 1,6–2,9–2,4 three times. Cholesterol increased throughout the study, but the peak of its rise was observed on day 9 – 39.2 %. The highest concentration of bilirubin was 4.4 times at the initial stage. Calcium and phosphorus tended to decrease in comparison with the control only at the end of the experiment by 41,4 and 46,1 %, respectively. The indicators of enzyme metabolism were strained and were high compared to the control analogues. On days 1–9–18 of the study, the ALT and AST levels were higher, respectively, in 2,0–2,1–2,2 and 2,6–2,7–2,8 three times. The content of alkaline phosphatase was the most significant on the 18th day of the experiment. The most effective result from complex treatment was obtained in the experimental group, where the chemotherapeutic agent enrofloxacin, hepatotropic – heptral, hormonal – dexamethasone and others were used. In the control group, where traditional therapy was used, the clinical status of the animals was normalized on day 17, in the experimental group – on day 12.

Cryptosporidiosis: An Emerging Zoonotic Disease of Global Public Health Concern

Cryptosporidiosis is an infectious emerging zoonotic protozoan disease occurring in both human and animal populations all over the world. Cryptosporidiosis is caused by Cryptosporidium, a tiny parasite that affects humans as well many species of animals. Although Cryptosporidium parvum and Cryptosporidium hominis (formerly known as C. parvum anthroponotic genotype or genotype 1) are the most common species causing disease in human beings, Cryptosporidium felis, Cryptosporidium meleagridis, Cryptosporidium canis, and Cryptosporidium muris infections have also been reported. Waterborne transmission via drinking water or a swimming pool is common, and outbreaks have been reported in several countries. The most common symptom of cryptosporidiosis in humans is watery diarrhea. People with weakened immune systems are more susceptible to contract serious, long-term, and even fatal infections. The laboratory endeavor is required to make a precise diagnosis of the disease. The demonstration of oocysts of Cryptosporidium in the fecal samples is still used as a simple, easy, and cost-effective technique for the diagnosis of disease. As a preventative and control measure, appropriate hygienic practices must be practiced everywhere. Furthermore, the veterinarians play a crucial role in the treatment of disease in domestic animals.

Effectiveness of albendazole-based anti-parasitic drugs under modern conditions

An important problem in veterinary medicine is still the control of parasitic diseases of farm and domestic animals. The market of antiparasitic drugs is widely represented by anthelmintics based on albendozole, both imported and domestic, but data on the effectiveness of these drugs are currently insufficient. The aim of the research was to determine in the comparative aspect the effectiveness of the use of antiparasitic drugs in different forms with the content of albendazole in different species of animals. In the previous stage of the studies the experimental animals were diagnosed with invasions. The most common infections in cattle were dictyocaulosis and fasciolosis; Trichostrongylus colubriformis, Cooperia oncophora, Oesophagostomum radiatum were not detected. No causative agent of Dictyocaulus vivaparus infection was detected in horses. The most common infection in dogs and cats was toxacariasis, and in mink the most common was infection wih Uncinaria spp. Along with this, no Taenia solium and Diphyllobothrium latum eggs were detected in dogs, and Opisthorchis felineus, Taenia solium, Diphyllobothrium latum were not detected in cats. After the use of preparative forms of albendazole in sick animals for 2–5 days, the presence of dead sexually mature nematodes and helminth eggs were noted in the feces of animals. The efficacy of albendazole drugs regarding helminthiasis in farm and domestic animals was 100%

Additional resource for diabetes diagnostics in animals and humans

Veterinary doctors often observe cases of unexplained elevated glucose and ketones in urine of domestic animals without any other signs of diabetes. We studies these effects from the standpoint of the phenomenon of interdependent conditions in animals and humans, described by T.V.Novosadyuk in 2000. She was the first to provide a theoretical and practical foundation for clinical cases of simultaneously developing similar diseases in domestic animals and their owners.&#x0D; During the last 5 years we studied health of humans in families where domestic animals are affected by the laboratory abnormalities described above. In vast majority of cases it has been found out that animal owners have diabetes mellitus of variable severity.&#x0D; At the same time there were no disorders of carbohydrate metabolism in animal owners in 11 cases. We recommended members of these families to undergo a specialized examination. In all of these cases latent diabetes mellitus was found in humans who had especially close relationships with animals. These findings led to initiation of treatment in humans.&#x0D; At the same time animals were treated with a collar with a linen sack attached containing Peganum Harmala 30 globules. Repeated laboratory tests were performed after one month of such treatment. Normalization of laboratory variables was observed in all of the cases.&#x0D; Based on the study results we developed an algorhythm of activities that helps to diagnose early and latent forms of diabetes mellitus in domestic animals and their owners. This algorhythm includes:&#x0D; - test for glucose and/or ketones in animal urine after correction of feeding and care defects.&#x0D; - blood and urine glucose tests in family members of animal owners. In cases of deviations from normal values we recommended them to consult appropriate specialists and begin treatment immediately.&#x0D; - animals are given collars with Peganum Harmala 30 globules in a linen sack attached.&#x0D; - granules are removed when laboratory values normalize in animals. Control urinalysis is performed every three months durign a year.&#x0D; This approach is especially useful in latent early forms of diabetes mellitus when abnormal findings in animals or their owners enable us to suspect a similar disease in another. &#x0D; In such a way, the suggested algorithm is effective for organization of preclinical diagnostics in both domestic animals and their owners. In the future it is possible to enrich not only veterinary but also medical practice with new options for effective patient aid by practical development of the use of animal and human interdependent conditions.

Concept of classical homeopathy in veterinary medicine

Attempts to use the principles of classical homeopathy in animal treatment encountered impossibility to select drugs according to the similarity principle due to lack of verbal contact. We suggested a new method of choosing homeopathic remedies according to the similarity principle based on the phenomenon of interdependent conditions in animals and their owners. The accumulated experience enabled us to confirm that this method is correct and productive, as well as the recommendations for diagnostics and treatment of different diseases in domestic animals.

An Appraisal of the Knowledge, Attitude and Practices of Abattoir Workers and Butchers on Bovine Tuberculosis in Kaduna Central Abattoir, Kaduna State, Nigeria

Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a chronic infectious and contagious zoonotic disease of domestic animals, wild animals and humans. This research involved abattoir workers who are personnel involved in the management of animals before and during the slaughtering process. Three hundred pretested, well-structured and closed-ended questionnaires were self-administered to abattoir workers made up of 13 Veterinarians, 8 Animal Scientists, 37 Para-veterinarians, 197 butchers and 45 unskilled labourers to determine the level of their knowledge, attitude and practices on tuberculosis. The study revealed that the abattoir workers in this study had low knowledge of bTB 32(10.6%). Their knowledge on the transmission pathways, prevention, control measures against bTB and its zoonotic importance were also sought. Detection of tubercle lesions of bTB in slaughtered cattle makes their potential source of infection to human and pose a public health risk. There is the need for enhanced public education and awareness campaigns among meat handlers and abattoir workers especially on the mode of transmission, aetiology and public health importance of bTB in Kaduna State