Decrease In Succinate Dehydrogenase Activity Research Articles

Effect of scorpion (Buthus minax, L. Koch) venom on succinic dehydrogenase and cholinesterase activity of mouse tissue.

The histochemical activities of succinic dehydrogenase and cholinesterase in mouse tissues after administration of scorpion ( Buthus minax ) venom have been studied. There were obvious changes in the enzyme activities, of the chronically treated animals, in the kidney, heart and liver. The decreases in cholinesterase activity were greatest in the white matter of the brain, in the portal tract and in some liver cells, while the spleen showed increased activity. A decrease in succinic dehydrogenase activity was observed in the kidney, heart, and liver of the chronically treated mice, while the spleen showed no change in the activity. The striated muscles of the acutely treated mice were fragmented and vacuolated with a loss of their striation. The intramuscular nerve axons became beaded and the subneural apparatus and their terminal expansions were smaller.

Metabolic changes induced in chickens by the administration of tremetol

Tremetol is a toxic extract of the White Snakeroot plant ( Eupatorium urticaefolium Reichard). The milk of cows grazing on Eupatorium-infested pastures causes “milk sickness”, characterized in part by ketoacidosis and changes in blood glucose levels. In this paper the metabolic effects of chronic administration of tremetol in the chick were studied. Blood glucose and blood free fatty acid levels respond with a biphasic pattern to tremetol administration, first rising above, then decreasing below the control values. In contrast, the blood levels of β-hydroxybutyrate show a sustained and major increase during the treatment period. Tremetol had no demonstrable effect on hepatic glycogen content in the treated group. Studies on hepatic enzyme levels revealed an almost total suppression of citrate synthase activity in the experimental group without a parallel decrease in succinate dehydrogenase activity. A selective toxic action on mitochondria resulting in an inability to effectively metabolize acetyl CoA is put forward as the possible cause of the ketoacidosis.

Effect of storage on succinic dehydrogenase and acid phosphatase activity in aortic and pulmonary valve homografts.

Succinic dehydrogenase and acid phosphatase activities have been measured in aortic and pulmonary valve homografts after various storage times in antibiotic media. There was a rapid decrease in succinic dehydrogenase activity within the first four days of storage. There was a gradual increase in acid phosphatase activity during storage, reaching a peak at 20 days.

Hereditary muscular hypertrophy in the bovine. I. Histological and biochemical characterization.

SummaryMuscles from three normal calves and three calves with hereditary muscular hypertrophy were biopsied at nine months of age and assayed histochemically for succinic dehydrogenase activity, and histologically for evidence of muscular degeneration. In addition, the biopsy samples were analyzed for total DNA, RNA, and protein. Blood samples from 13 normal and 12 hypertrophied animals were taken at 12 months of age and assayed for creatine phosphokinase activity. Histologically, no degenerative changes were detected in hypertrophied animals. No significant differences were found in concentrations of DNA, RNA, protein, or in serum creatine phosphokinase activity between normal and hypertrophied animals. There was a significant decrease in succinic dehydrogenase activity of the hypertrophied muscle. There were fewer reacting fibers, and the activity was less in those fibers from hypertrophied muscle which did react for succinic dehydrogenase. It is concluded that degeneration is not a part of this syndrom...

Enzyme histochemistry of human oral carcinoma

Histochemical techniques for the determination of lactic dehydrogenase activity and succinic dehydrogenase activity were applied to the study of ten cases of epidermoid carcinoma of oral mucosa. A notable increase in lactic dehydrogenase activity was found, as well as a decrease in succinic dehydrogenase activity, when oral carcinoma was compared with normal oral mucosa.

Ultrastructural and cytochemical changes in tissue culture infected with type 5 adenovirus

Cytochemical and electron microscopic methods were used to study changes in the HEp-2 tissue culture infected with type 5 adenovirus. The infected tissue culture was noted to have a considerable decrease in succinate dehydrogenase activity in 24, 48 and 72 h. The activity of DPN- and TPN-diaphorases somewhat increased 10 h after infection and sharply decreased only on the 3rd day of investigation. The activity of acid and alkaline phosphatases did not change substantially within the first 48 h of infection and somewhat declined in 72 h. Non-simultaneous decrease in the activity of succinate dehydrogenase and DPN- and TPN-diaphorases was connected with the morphological characteristics of the localization. Since the virustiter considerably increased on the 8th day of investigation it is supposed that these enzymes are not decisive for virus reproduction. The infected tissue culture was noted to have a change in glycogen deposition manifested by lyoglycogen. Feulgen-positive inclusions, constantly encountered in infected tissue culture have a complex structure: they give an intensive reaction not only to DNA but also to total protein and sulfhydryl groups.