Cotton Pellet Granuloma Test Research Articles

Analgesic and Anti-Inflammatory Activity: A Comprehensive Review on Invitro and In Vivo Screening Methods

Modern pharmacotherapy includes analgesic and anti-inflammatory medicines as essential components to relieve pain and inflammation brought on by a variety of medical diseases. Robust screening techniques are essential for the identification of possible candidates with appropriate safety and effectiveness profiles in the search and development of new analgesic and anti-inflammatory medications. This study looks at the many screening methods used in preclinical studies to assess new drugs' analgesic and anti-inflammatory quality. Conventional techniques like the tail flick, hot plate, and writhing’s tests measure analgesic activity by having animals respond to unpleasant stimuli. Comparably, anti-inflammatory activity is frequently assessed using assays like the cotton pellet granuloma test, which gauges tissue granuloma formation, and the carrageenan-induced paw edema model, which measures inflammation. These traditional techniques offer insightful information about the pharmacological effects of test substances. Despite the wide range of screening techniques available, each strategy has advantages and disadvantages. Preclinical studies are more reliable and have higher predictive value when various assays and techniques are integrated into a tiered screening strategy. Furthermore, the successful translation of preclinical findings to human applications depends on taking into account translational variables including species differences and clinical relevancies. As a result, choosing the right screening techniques is critical to the effective identification and characterization of new analgesic and anti-inflammatory drugs.

Evaluation of Wound Healing and Anti-Inflammatory Activities of 80% Methanol Crude Extract and Solvent Fractions of Trichilia dregeana Sond (Meliaceae) Leaves in Mice.

The leaves of Trichilia dregeana Sond are traditionally used to treat wounds. Even though there have been in vitro studies and claims supporting wound healing, there are no scientific data on in vivo wound healing and anti-inflammatory activities of the leaves of T. dregeana. This study aimed to evaluate wound healing and anti-inflammatory activity of 80% methanol crude extract and solvent fractions of T. dregeana in mice. The leaves of T. dregeana were dried, ground, and macerated with 80% methanol three times successively. The crude extract was fractioned by water, ethyl acetate, and hexane separately. The acute dermal and oral toxicity tests were done by applying 2000 mg/kg of 10% (w/w) crude extract ointment (CEO) topically and 2000 mg/kg of crude extract orally, respectively. The wound healing activity of the crude extract was evaluated on excision, incision, and burn wound models while the fractions were evaluated only on excision wound model. The anti-inflammatory activity of the crude extract was evaluated on xylene-induced ear edema and cotton pellet granuloma tests. Both acute dermal and oral toxicity tests were found to be safe after topical application of 2000 mg/kg of 10% (w/w) CEO and oral administration of 2000 mg/kg of crude extract suspension, respectively. Both 5% and 10% (w/w) CEO produced significant (p < 0.001) wound contraction and period of epithelialization from day 4 onwards as compared to simple ointment (SO) on both excision and burn wounds. The tensile strength was increased significantly (p < 0.001) for the CEO-treated mice as compared to both untreated and SO groups. The crude extract also showed anti-inflammatory activity at 100, 200, and 400 mg/kg by inhibiting ear edema, exudate, and granuloma formation as compared to the SO group. The increase in wound contraction, reduction in period of epithelialization, and increase in tensile strength support the traditional claims of T. dregeana for wound healing.

Investigation of the anti-inflammatory and analgesic activities of promising pyrazole derivative

The development of new COX-2 inhibitors with analgesic and anti-inflammatory efficacy as well as minimal gastrointestinal, renal and cardiovascular toxicity, is of vital importance to patients suffering from chronic course pain and inflammatory conditions. This study aims at evaluating the therapeutic activity and adverse drug reactions associated with the use of the newly synthesized pyrazole derivative, compound AD732, E-4-[3-(4-methylphenyl)-5-hydroxyliminomethyl-1H-pyrazol-1-yl]benzenesulfonamide, as compared to indomethacin and celecoxib as standard agents. Anti-inflammatory activity was assessed using carrageenan-induced rat paw edema and cotton pellet granuloma tests; formalin-induced hyperalgesia and hot plate tests were done to study analgesic activity. In vitro tests to determine COX-1/COX-2 selectivity and assessment of renal and gastric toxicity upon acute exposure to AD732 were also conducted. Compound AD732 exhibited promising results; higher anti-inflammatory and analgesic effects compared to standard agents, coupled with the absence of ulcerogenic effects and minimal detrimental effects on renal function. Additionally, compound AD732 was a less potent inhibitor of COX-2 in vitro than celecoxib, which may indicate lower potential cardiovascular toxicity. It may be concluded that compound AD732 appears to be a safer and more effective molecule with promising potential for the management of pain and inflammation.

Iridoid glycosides from Morinda officinalis How. exert anti-inflammatory and anti-arthritic effects through inactivating MAPK and NF-\u03baB signaling pathways

BackgroundThe root of Morinda officinalis How. (MO, the family of Rubiaceae) has long been used to treat inflammatory diseases in China and other eastern Asian countries, and iridoid glycosides extracted from MO (MOIG) are believed to contribute to this anti-inflammatory effect. However, the mechanism underlying the anti-inflammatory and anti-arthritic activities of MOIG has not been elucidated. The aim of the present study was to determine how MOIG exerted anti-inflammatory and anti-arthritic effects in vivo and in RAW 264.7 macrophages.MethodsMOIG were enriched by XDA-1 macroporous resin. The maximum feasible dose method was adopted to evaluate its acute toxicity. The analgesic effect of MOIG was evaluated by acetic acid writhing test and the anti-inflammatory effect was evaluated by cotton-pellet granuloma test in rats and air pouch granuloma test in mice. The anti-arthritic effect was evaluated by establishing an adjuvant arthritis model induced by Complete Freund’s Adjuvant (CFA). The viability of the cultured RAW 264.7 macrophages was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. The anti-inflammatory activity was evaluated by measuring NO, IL-1β, IL-6 and TNF-α levels in LPS-stimulated RAW 264.7 cells. The protein level of inflammatory responsive genes was evaluated by Western blot analysis.ResultsMOIG had no significant toxicity at maximum feasible dose of 22.5 g/kg. MO extracts and MOIG (50,100 and 200 mg/kg) all evoked a significantly inhibitory effects on the frequency of twisting induced by acetic acid in mice compared with the model control group. Administration of MO extracts and MOIG markedly decreased the dry and wet weight of cotton pellet granuloma in rats and air pouch granuloma in mice. MOIG significantly attenuated the paw swelling and decreased the arthritic score, weight loss, spleen index, and the serum level of inflammatory factors IL-1β, IL-6 and IL-17a in CFA-induced arthritic rats. MOIG inhibited the production of inflammatory cytokines in LPS-stimulated RAW264.7 cells, and the expressions of iNOS, COX-2 and proteins related to MAPK and NF-κB signaling pathways in LPS-stimulated RAW 264.7 macrophages.ConclusionMOIG exerted anti-inflammatory and anti-arthritic activities through inactivating MAPK and NF-κB signaling pathways, and this finding may provide a sound experimental basis for the clinical treatment of rheumatoid arthritis with MOIG.

Anti-Inflammatory and Antinociceptive Activities of the Ethanolic Extract of Propolis in Male Mice and Rats

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) are usually used to alleviate pain and Inflammation. The widespread use of NSAIDs has caused the risk and side effects of these drugs commonly increase. Objectives: This study aimed to examine the anti-inflammatory and antinociceptive activities of ethanolic extract of propolis (EEP) in male mice and rats. Methods: In this experimental study, EEP was prepared and administered in doses of 100, 200, and 400 mg/kg of body weight. The anti-inflammatory potential was assessed by inflammatory models of xylene-induced ear edema and cotton pellet granuloma tests, whereas the antinociceptive potential was assessed by formalin and acetic acid-induced writhing methods. Results: The EEP at doses of 100 (P < 0.05), 200 (P < 0.001) and 400 mg/kg (P < 0.001) significantly diminished the foundation of edema caused by xylene. A significant decrease was seen in granuloma weight in EEP at doses of 200 (P < 0.001) and 400 mg/kg (P < 0.001). The extract caused a significant decrease in licking time at first phase in EEP at 100 (P < 0.01), 200 (P < 0.001), and 400 mg/kg (P < 0.001). A significant decrease was observed (P < 0.001) in licking time at the second phase in EEP at doses of 100, 200, and 400 mg/kg. In the writhing model, a significant decrease was observed in the number of writhes in EEP at doses of 100 (P < 0.05), 200 (P < 0.001), and 400 mg/kg (P < 0.001). Conclusions: The findings of this research showed that the ethanolic extract of propolis has considerable anti-inflammatory and antinociceptive activities.

Methanol extract of smokeless tobacco alters inflammation and nociception process in animal models

Purpose: To investigate the inflammatory and nociceptive alterations due to the use of Nicotiana tabacum or smokeless tobacco (MEST) owing to the fact that it is used by some people to relieve dental pain.Methods: Hepatic biochemical indicators and thiobarbituric acid reactive substances assay were used to assess MEST toxicity and pharmacological doses selection. The effects on inflammation of different pharmacological doses (100, 200 and 500 mg/kg i.p.) of MEST were evaluated using xylene-induced ear edema and cotton pellet granuloma tests. Indomethacin (10 mg/kg, i.p.) was used as positive standard drug, whereas the vehicle 0.5 % CMC treated group was considered as negative control. Acetic acid-induced abdominal contraction test and formalin-induced hind paw licking model were utilized to assess the role of MEST in nociception. Indomethacin (10 mg/kg i.p.) and diclofenac sodium (10 mg/kg i.p.) were used as positives standard drugs. The vehicle used was 0.5% CMC which served as the negative control.Results: MEST (50 %, 200 mg/kg) and indomethacin (47.5 %) both elicited a significant (p < 0.001) anti-edematogenic effect on xylene-induced ear edema. MEST also showed a significant (p < 0.001) inhibitory effect on granuloma formation at all administered doses as compared to the untreated groups which was comparable to standard drug indomethacin. The number of acetic acid induced writhings was observed to be significantly increased (p < 0.001) by MEST at all doses, unlike diclofenac that led to significant reduction (p < 0.001) in the number of writhings, when compared to the untreated group. MEST also showed a significant (p < 0.05) dose-dependent reduction of the hind paw licking caused by formalin when compared to the vehicle control.Conclusion: These results signify that administration of MEST induces inflammatory and nociceptive alterations. However, the extract is not recommended for dental pain due to its other toxic effects that have previously been reported.Keywords: Nicotiana tabacum, Smokeless tobacco, Inflammation, Nociception

“EVALUATION OF GALPHIMIA GLAUCA STEM METHANOL EXTRACT FRACTIONS FOR ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES”

Objective: This current investigation assesses in vivo central and peripheral analgesic effects and anti-inflammatory properties of fractions obtained from Galphimia glauca (GG) stem methanol extract.&#x0D; Methods: The laboratory models such as Swiss albino mice and Wistar albino rats were employed in the studies. The GG stem methanol extract was subjected to fractionation with solvents such as hexane, chloroform, ethyl acetate, and methanol. Orally, the dose range of 100, 200, and 400 mg/kg was given for 1 day for evaluating analgesic (hotplate test, tail clip test, writhing test, and formalin test) and weekdays for assessing anti-inflammatory activity (carrageenan and cotton pellet test methods), respectively. The experimental studies were further conducted for determining the involvement of central and peripheral receptor actions in the analgesic activity of the extract by prechallenging it with naloxone and acetic acid, respectively. The in vivo anti-inflammatory studies were conducted using carrageenan-induced rat paw edema model and cotton pellet granuloma test.&#x0D; Results: The LD50 of the extract was found to be &gt;2000 mg/kg b.w. The methanol fraction of 400 mg/kg dose exhibited significant (p≤0.001) and dose-dependent analgesic and anti-inflammatory activity. It also exhibited central and peripheral analgesic actions when treated with naloxone and acetic acid, respectively.&#x0D; Conclusion: The results revealed that the stem methanol fraction has more potential in terms of analgesic and anti-inflammatory properties.

&lt;em&gt;In vitro&lt;/em&gt; antioxidant and &lt;em&gt;in vivo&lt;/em&gt; anti-inflammatory activity of &lt;em&gt;Curcuma albiflora&lt;/em&gt; Thw.

Curcuma albiflora Thw. is a poorly explored plant which is termed as Harankaha locally. Among other Harankaha plants, C. albiflora is threaten and restricted to Sabaragamuwa province only. Therefore, current study was conducted to quantify phytochemicals in terms of total phenolic content (TPC), total flavonoid content (TFC) and prove or disprove its antioxidant and anti-inflammatory activity using ferric reducing antioxidant power (FRAP), DPPH, ABTS+, and oxygen radical absorbance capacity (ORAC) assays, and carrageenan-induced paw oedema, formaldehyde-induced paw oedema, and cotton pellet induced granuloma tests in Wistar rats respectively. Whole plant extract from composite samples was prepared using 50% ethanol (in water) and 50% dichloromethane (in methanol) as solvents mixture by continuous extraction (6 h). Antioxidant values of TPC, TFC, and FRAP were 31.25 ± 1.48, 11.22 ± 0.13, and 17.90 ± 0.38 mg/g respectively. IC50 values (ppm) of DPPH and ABTS+ were 827.78 ± 6.06, and 188.84 ± 2.99 respectively. ORAC value was 128.10 ± 3.29 mg/g. The correlation of TFC was shown that it may link to flavonoid content. Since, C. albiflora inhibited (61% at 1h) 1st phase in the acute model, and at the late phase on sub-chronic model considering insignificant on cotton pellet granuloma test was shown that its anti-inflammatory activity (as 19.5% on 400 mg/kg) may not relate on prostaglandin. Further experiments are warrant to identify a mechanism of anti-inflammatory activity of C. albiflora.

Antiasthma activity of Eucalyptus grandis essential oil and its main constituent: Vasorelaxant effect on aortic smooth muscle isolated from nomotensive rats

Asthma is a chronic inflammatory disorder of the airways. It is characterized by an inflammation of the airways causing dysfunction. Asthma is associated with widespread airflow obstruction and increase in airway responsiveness to a variety of stimuli. An asthma attack is accompanied by wheezing, shortness of breath, chest tightness and coughing. The anti-asthmatic and anti-cough activities of the essential oils and 1,8-cineole on rats were assessed. These activities were induced and challenged with histamine and acetylcholine using an ultrasonic nebulizer for asthma and exposure to ammonia for coughs. Anti-inflammatory activities of the essential oils and 1,8-cineole were determined using the cotton pellet granuloma test. Muscle contraction studies where carried out using the vascular reactivity on aortic smooth muscle. The oils exhibited concentration dependent anti-asthma and anti-cough activities. Significantly, 1,8-cineole isolated and purified from the essential oil showed a concentration dependent anti-inflammatory, anti-cough and anti-asthma activity and The essential oils showed vasorelaxant activity. The ability of the essential oils and 1,8-cineole to directly dilate smooth muscle and relieve the stress of bronchial contraction and obstruction of the airways passage validates the use of Eucalyptus grandis as an anti-asthmatic and anti-cough agent. It is concluded that the essential oils have the potential to be used as an anti-asthma and anti-cough therapy. This study also justifies its use by traditional healers in the treatment of asthma and coughs in Zulu folklore medicine.

Preliminary screening of &lt;em&gt;Waltheria indica&lt;/em&gt; (L.) plant for its anti-inflammatory activity

&lt;p dir="ltr"&gt;&lt;span&gt;The investigation on anti-inflammatory activity of the various extract of &lt;/span&gt;&lt;span&gt;Waltheria indica &lt;/span&gt;&lt;span&gt;L. &lt;/span&gt;&lt;span&gt;was reported to find out the pharmacological basis for its ethnomedical use. &lt;/span&gt;&lt;span&gt;The anti-inflammatory activity of the pet ether (PEW) and methanol (MEW) extracts of the leaves of &lt;/span&gt;&lt;span&gt;Waltheria indica &lt;/span&gt;&lt;span&gt;L. (&lt;/span&gt;&lt;span&gt;Malvaceae&lt;/span&gt;&lt;span&gt;)&lt;/span&gt;&lt;span&gt;were evaluated by using in vivo (Carrageenan &amp;amp; histamine induced rat paw edema, cotton pellet granuloma test) models. It was observed that, all the extracts showed significant activity in the in-vivo model at the dose of 500 mg/kg b.w. orally, when compared with control and standard drugs. Of the two extracts tested, methanol extract MEW showed most significant activity well in comparison to the standard drug. Therefore, present study suggests, potential of leaves of &lt;/span&gt;&lt;span&gt;Waltheria indica &lt;/span&gt;&lt;span&gt;L. in both models of acute and chronic inflammation.&lt;/span&gt;&lt;/p&gt;&lt;p dir="ltr"&gt; &lt;/p&gt;

Anti-Inflammatory Activity of Ethanolic Leaf Extract of Dalbergia Sissoo In Vitro and In Vivo

The anti inflammatory activity of Dalbergia sissoo ethanolic leaf extract DSELE was evaluated from the tests of HRBC human red blood cell membrane stabilization in vitro and cotton pellet granuloma test in mice Inhibition of protein denaturation by DSELE was studied to assess anti arthritic activity Extract of D sissoo in different solvents were evaluated for presence of phytoconstituents in the plant From the results DSELE showed appreciable HRBC membrane stabilization activity DSELE at and g ml concentration inhibited protein denaturation and per cent respectively In cotton pellet granuloma test in mice DSELE showed dose dependant and significant P

Anti-inflammatory, Anti-arthritic and Analgesic Effect of the Herbal Extract Made fromBacopa monnieriis,Cassia fistulaandPhyllanthus polyphyllus

Anti-inflammatory, anti-arthritic and analgesic activity of each herbal extract, which is extracted from Bacopa monnieriis, Cassia fistula and Phyllanthus polyphyllus, respectively. The treatment of herbal extract exhibited anti-inflammatory effect as a dose-dependent manner, from 1.25mg/kg to 12.5mg/kg, in acute inflammatory models (carrageen and egg-albumin induced rat hind paw edema). It also elicited significant anti-inflammatory activity in chronic inflammatory models (cotton pellet granuloma and Freund`s adjuvant induced polyarthritis in rat). In cotton pellet granuloma test, the extract exhibited the inhibitory effect of 23 and 57% at the dose of 6.25 and 12.5 mg/kg, respectively. In Freund`s adjuvant induced model, the treatment of the extract of 1.25, 6.25 and 12.5 mg/kg showed the inhibitory effect of 23, 56 and 66% at 8 days, respectively. In the acetic acid-induced model, the extract significantly reduced abdominal writhing in mice when compared to the control group, reducing the mean number of writhing from 41 ± 2 in the control group to 17 ± 3 and 15 ± 2 at the dose of 6.25 and 12.5 mg/kg. From these experiments, the extract, which was extracted from the combination of Bacopa monnieriis, Cassia fistula and Phyllanthus polyphyllus, (w/w/w = 1/2/1) is surprisingly found a significant analgesic and anti-inflammatory activity.

Low dose aspirin like analgesic and anti-inflammatory activities of mono-hydroxybenzoic acids in stressed rodents

AimsTo compare analgesic and anti-inflammatory activities of aspirin and mono-hydroxybenzoic acids after their daily oral doses. Main methodsEfficacies of repeated daily stress response suppressing low oral doses (20mg/kg) of aspirin and 2-, 3-, and 4-hydroxybenzoic acids in mice hot plate test for centrally acting analgesics, and in acetic acid induced writing test were compared. Effects of their same daily doses and treatment regimen in cotton pellet granuloma and carrageenan edema test for anti-inflammatory drugs in stressed rats were compared in a second experiment. Effects of treatments on body weights, basal rectal temperatures, organ weights and plasma glucose, insulin and cortisol levels in stressed animals were compared also. Key findingsAlthough stress response suppressing effects of aspirin and all the three hydroxybenzoic acids in both mice and rats were almost equal, effectiveness of 3- and 4-hydroxybenzoic acids as analgesic and anti-inflammatory agents were lower than those of aspirin or salicylic acid. SignificanceObservations made after single oral doses of aspirin or of mono-hydroxybenzoic acids are not very reliable predictors of their pharmacologically interesting bioactivity profiles and efficacies. Prostaglandin synthesis inhibition is not involved in low dose anti-inflammatory activities of 3- and 4-hydroxybenzoic acids. After their repeated daily low oral doses they are almost as potent stress response desensitizers as aspirin or salicylic acid.

Anti-inflammatory activity of fatty extract of Vitalleria paradoxa Kernel (Shea butter) and pattern of its clinical use in arthritis in Enugu, South East Nigeria

Background: The fatty seed extract of Vitellaria paradoxa Kernel (Sapotaceae) commonly called “Shea butter”, is a popular remedy for arthritis in west Africa. This study investigated the knowledge, prevalence and pattern of use of Shea butter in clinically diagnosed arthritic patients as well as its effects on acute (topical and systemic) and chronic inflammation in rodents. Methods: Knowledge, prevalence and pattern of use were determined using pre-tested questionnaire in clinically diagnosed patients whereas the anti-inflammatory activity was studied using xylene-induced mouse ear edema, carrageenan-induced rat paw edema, formaldehyde-induced arthritis in rats and cotton pellet granuloma test in rats. Results: The results showed that of the 164 respondents, 94.1% know about Shea butter and 59.6% have used it mainly as a massage ointment once or twice daily. However, 73.7% of the users combine this remedy with analgesics to achieve relief. The pharmacological tests showed that topical application of Shea butter inhibited acute edema of the mouse ear. Systemic oral administration caused significant (p<0.05) suppression of the development of systemic acute edema of the rat paw in a non-dose related manner. Twice daily topical application of Shea butter significantly (p<0.05) inhibited the edematous response to formaldehyde arthritis whereas once daily administration was not effective. Shea butter also caused a significant (p<0.05) non-dose related inhibition of granuloma tissue formation on implanted cotton pellets. Conclusions: These findings provide a scientific rationale for the use of Shea butter in treatment of disorders of inflammation in traditional medicine.

Effects of the Ethanolic Extract of Bulbostylis coleotricha (Hochst.Exa. Rich.) on Inflammation in Adult Wistar Rats

Bulbostylis coleotricha (family Cyperaceae) is a stem-tufted plant found in tropical region of Africa. It is locally used in treating various ailments among the African folks. Despite the high esteem this plant is held in Africa, there is dearth of scientific studies on B. coleotricha. This, in addition to the alarming increase in inflammatory diseases around the world, makes the present study to focus on the effect of the B. coleotricha extract on laboratory induced inflammation. Twenty-five adult Wistar rats, weighing between 225-287g were used for this experiment, and were randomly divided into five groups of five rats per group. The groups were labelled and treated as follow: Group 1, the negative control group, was treated with 10 ml/kg body weight (b. w.) of distilled water. Groups 2, 3 and 4, the treated groups, were treated with 25 mg, 50 mg and 100 mg/kg b. w. of ethanolic extract of B. coleotricha respectively. Doses were based on the local information on B. coleotricha usages. Group 5, the positive control group, was treated with 5mg/kg b. w. of indomethacin. In all the groups, the effects of all treatments were checked on induced inflammation using cotton pellet granuloma and carrageenan induced paw oedema model. The results in the two models used showed that ethanolic extract of B. coleotricha significantly inhibited inflammation in the treated groups. In the cotton pellet granuloma test the extract at the dose of 100 mg reduced granuloma formation from 30.2 ± 2.4 mg to 11.2 ± 2.6 mg reduced paw oedema from 7.0 ± 1.7 mm to 2.4 ± 0.8 mm (control is 100 mg/kg). In conclusion, this study established that Bulbostylis coleotricha plant is effective in the treatment of inflammation and can be explored in the treatment of various diseases with underlying inflammation.Keywords: Bulbostylis coleotricha, Inflammation, Cotton Pellet Granuloma, Carrageenan-induced Paw Oedema

Anti-inflammatory activity of phlomisoside F isolated from Phlomis younghusbandii Mukerjee

This study was designed to investigate the anti-inflammatory effect of phlomisoside F (PMF) isolated from Phlomis younghusbandii and to explore the possible pharmacological mechanisms. Anti-inflammatory effects of PMF were evaluated by using carrageenan-induced rat paw edema test, dimethylbenzen-induced ear edema test, acetic acid-induced vascular permeability and cotton pellet granuloma test. Furthermore, the releases of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) were determined by ELISA. To explore the potential mechanisms, expressions of iNOS and COX-2 were determined by quantitative real-time PCR and western blotting assays. In addition, the expressions of nuclear p65, cytosolic p65, IκB, p38, p-p38, p-ERK1/2, ERK1/2, JNK and p-JNK were determined by western blotting assay. Our results indicated that PMF administered orally could not only significantly decrease rat paw edema in rats and ear edema in mice, but also reduce the vascular permeability in mice and granuloma weights in rats. In vitro, the releases of LPS-induced pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) and enzymes (iNOS and COX-2) were decreased significantly by PMF treatment in RAW 264.7 cells. In addition, the LPS-induced up-regulations of nuclear p65, p38, p-p38, p-ERK1/2, JNK and p-JNK proteins in RAW 264.7 cells significantly decreased by PMF, and expressions of cytosolic p65 and IκB were obviously up-regulated after treatment with PMF. In conclusion, we suggested that the PMF is a promising potential anti-inflammatory drug, and PMF could down-regulate expressions of pro-inflammatory cytokines and mediators by inhibiting the NF-κB/MAPK pathways.

Anti-inflammatory Activities of the Leaf Chloroform Extract of Palisota hirsuta

Objective: To investigate the anti-inflammatory activity of the chloroform leaf extract of Palisota hirsuta (CLEPH). Methods: CLEPH was evaluated for anti-inflammatory activity using standard experimental models of inflammation namely: Croton oil-induced mouse ear edema, carrageenan-induced rat paw edema and cotton pellet granuloma test. The activities of the extract at different doses were compared to indomethacin, a standard anti-inflammatory drug. Results: In all the experiments, the chloroform extract of P. hirsuta showed in a dose-dependent manner, anti-inflammatory effects which were significant (p˂0.05) and comparable to those of indomethacin. Conclusion: The results showed that CLEPH possess significant anti-inflammatory activity. This supports its use as an anti-inflammatory recipe in folk medicine. Original Research Article Kelechi and Uzoma; JAMPS, 2(2): 57-63, 2015; Article no.JAMPS.2015.008 58

Antiinflammatory Activity of Hot Water Infusion of Nyctanthes arbo-tristis Flowers.

In Sri Lankan ethnomedicate it is claimed the flowers of Nyctanthes arbo-tristis is effective in the treatment of inflammatory conditions but this has not been scientifically validated. This experiment was carried to investigate the antinflammatory potential of hot water infusion of Nyctanthes arbo-tristis flowers. Oral antiinflammatory activity of hot water infusion of Nyctanthes arbo-tristis flowers (concentrations: 3.75, 7.5, 12.5 and 18.75 mg/kg) was assessed in rats using both acute (carrageenan-induced paw oedema assay) and chronic (formaldehyde induced-paw oedema and cotton pellet-granuloma tests) inflammatory models. In an attempt to investigate its mode of action, antihistamine activity (by wheal test), inhibition of prostaglandin synthesis (by enteropooling test), inhibition of Tumor necrosis factorα secretion (using human mononuclear cells), and suppression of vascular permeability (acetic acid-induced vascular permeability test) and cytotoxicity (Evans blue test) were assessed. In the carrageenan-induced paw oedema test, hot water infusion simultaneously suppressed both initial and late stages of inflammation in an inversely dose related manner. Hot water infusion also inhibited paw oedema in formalin and cotton pellet granuloma tests. In addition, this infusion exhibited marked anti histamine activity, prostaglandin synthesis inhibition and suppression of vascular permeability. These findings scientifically support the traditional use of Nyctanthes arbo-tristis flowers in treatment of inflammatory conditions.

Studies on the antiinflammatory, antinociceptive and antimicrobial activities of Combretum calobotrys (Combretaceae) leaf

The methanol:dichloromethane extract of Combretum calobotrys Engl & Diels (Combretaceae) leaves (CCE) was subjected to solvent-guided fractionation to yield the hexane (HF), dichloromethane (DF) and methanol (MF) fractions. The anti-inflammatory effects of the extract and fractions were evaluated in rodents using xylene-induced topical ear edema, carrageenan-induced pedal edema, formaldehyde-induced arthritis and cotton pellet granuloma tests, respectively. The extract and fractions were screened for analgesic activity using acetic acid induced writhing and formalin test in rodents. They were subjected to antimicrobial assay and also analysed for phytochemical constituents, while the CCE was subjected to acute toxicity (Lethal dosage, LD50) test using standard procedures. The extract and fractions (200 and 400 mg/kg) significantly (P 5,000 mg/kg. The results indicate that C. calobotrys leaf possess anti-inflammatory, analgesic and antimicrobial activities. Key words: Combretum calobotrys, anti-inflammatory, analgesic, abdominal writhing, formalin test, agar dilution.

Evaluation of anti-inflammatory activity of the hexane extract of seeds of Byrsonima crassifolia in experimental animal models

The effect of extracts on inflammation were studied in formaldehyde, 12-O-tetradecanoylphorbol (TPA), carrageenan and histamine induced edema. In addition, cotton pellet granuloma test was used to investigate the effects of NS on inflammation. The hexane extract (NS) significantly inhibited paw edema induced by carrageenan, formaldehyde and histamine as well as ear edema induced by TPA in rat after oral or topical administration at doses of 50, 100 and 200mg/kg. The antiedema potency of hexane extract was compared with indomethacin, dexamethasone and diclofenac sodium as standard drugs indicated that extract had good anti-inflammatory activity. Taken together, these results suggest that NS exerts anti-inflammatory activity with the partial contribution of inhibitory action of some inflammatory responses. In conclusion, hexane extract from seeds of Byrsonima crassifolia effectively controls acute and chronic inflammation in experimental models, which can be mediated by reducing the release of mediators and weakening the inflammation effects of these mediators.