Extract Of Coriandrum Sativum Research Articles

Efficacy of herbal mucoadhesive gel with extracts of Coriandrum sativum and curcumin as local drug delivery in the treatment of chronic periodontitis - A clinico-microbiological trial

Context: Herbal products are the recent advancement in the field of alternative medicines for the management of chronic periodontitis. They can be used as local drug delivery (LDD) agents over conventional antibiotics because of their extensive natural activity, advanced safety margin, and inferior costs. The present study evaluates the efficacy of one such indigenously prepared herbal gel containing extracts of Coriandrum sativum and curcumin in chronic periodontitis patients. Subjects and Methods: A total of 18 patients diagnosed with chronic periodontitis were included in this randomized clinical trial. The selected 72 pockets were equally divided into experimental and control groups. Full mouth scaling and root planing (SRP) was done. The experimental site received indigenously prepared herbal LDD gel containing combined extracts of C. sativum and curcumin, and the other side received a placebo gel. The periodontal parameters assessed were plaque index, papillary bleeding index, periodontal pocket depth (PPD), and clinical attachment level (CAL) at baseline, 1 month, and 3 months. Microbiological parameters for anaerobic analysis were assessed by colony-forming units at baseline and 3 months. Results: SPSS software analyzed all the data by repeated measure analysis of variance, paired t-test, and sample t-test with the difference of P Conclusion: Locally delivered combined extracts of C. sativum and curcumin gel was more effective in reducing periodontal clinical parameter when used as an adjunct to SRP in the treatment of chronic periodontitis.

Study on synergistic action of Coriandrum sativum seed extracts on antibiotics against multidrug resistant P. aeruginosa

Increase in resistance of antibiotic against P. aeruginosa, the antibiotic treatment therapy fail to exterminate the infection. The approach of new strategy is must to deal with multidrug resistant P. aeruginosa. In the present study total 300 isolates from different clinical samples (Urine, Blood, Sputum, and Pus) were studied for isolation, identification and analysis of resistance pattern against 22 antibiotics. Total 5 most resistant isolates (U004, P017, P078, U105, and U237) were screened to search for synergistic approach of Coriandrum sativum extract prepared by both Soxhlet method and Maceration process in different solvents; petroleum ether, chloroform, acetone, methanol and water. Encouraging results were obtained where HDME extract could synergise the activity of total 8 antibiotics that are; MZ, CPZ, CTX, PB, LE, DOR, AZ and CAZ. Thus the present study provides and landmark approach to deal with multidrug resistant P. aeruginosa by synergistic action of antibiotic and Coriandrum sativum extract.

The Possibility of Using Zinc Oxide Nano Particles for Treatment of Bovine-Subclinical Mastitis, in Vitro Antibacterial Activity

Bovine mastitis is an inflammation of mammary gland parenchyma in cows. It is caused by multiple pathogens that result in economic losses because of the reduction in milk production and poor quality of the milk. It is commonly caused by bacteria that multiply in the mammary gland and regional lymph nodes, damaging the mammary parenchyma. The present study focuses on the bovine mastitis treating capacity of zinc oxide nanoparticles by examining in vitro antibacterial activity, MIC and MBC against pathogens isolated and identified from sub-clinical mastitis in dairy cows. Zinc oxide nanoparticles were synthesized both chemically and green methods from a plant extract of Coriandrum sativum and characterized for phase and microstructural analysis. The milk sample from subclinical mastitis cows was collected from the veterinary hospital. The bacteriological examination revealed the presence of S. aureus and E. coli . The antibacterial activity of the zinc oxide nanoparticle was evaluated at 650, 850, 1300, 1700, 1950 and 2500 µg/ml concentrations and the minimum inhibitory concentration was found to be 650 µg/ml and 850 µg/ml for S.aureus and E.coli respectively, and minimum bactericidal concentration was 650 µg/ml and 1700 µg/ml for S. aureus and E. coli respectively. Keywords: Antibacterial activity, bovine subclinical Mastitis, Coriandrum sativum, chemical synthesis, green synthesis, minimum inhibitory concentration, minimum bactericidal concentration, zinc oxide nanoparticles DOI : 10.7176/CMR/11-10-01 Publication date: December 31 st 2019

Nephroprotective Screening of Coriandrum sativum L. Leaves Against Gentamicin Induced Renaltoxicity in Wistar Albino Rats

The whole plant of Coriandrum sativum L. is used in variety of diseases including renal ailments throughout the world. The aim of this study was to find out the nephroprotective activity of Coriandrum sativum L. against Gentamicin (GM) induced renal toxicity. Sixty Wistar albino rats were divided into ten groups. GM was injected i.p, at the dose of 100 mg/kg for 10 consecutive days for inducing nephrotoxicity. Metformin (MF) was given orally at two different doses i.e., 50 mg/kg and 100 mg/kg for 10 days either after inducing nephrotoxicity or along with nephrotoxicity. The fresh leaf extract of Coriandrum sativum L. was given orally at the dose of 2 ml/day for 28 day either with nephrotoxicity or after inducing the nephrotoxicity . The ethanolic extract of plant was given orally at the dose of 200 mg/kg/day for 28 day either with nephrotoxicity or after inducing the nephrotoxicity. The study indicate that C. sativum L. (fresh leaf juice as well as ethanolic extract) were showed more appreciably improvement on the kidney as compare to treatment with MF (100 as well as 50 mg/kg/day) when these treatment were given along with GM toxicity as well as after inducing the toxicity by GM. The results of this study revealed that C. sativum L. have free radical scavenging potency against increased production of reactive oxygen species by GM. Coriandrum sativum L. is more effective than MF.

Dietary effects of Coriandrum sativum extract on growth performance, physiological and innate immune responses and resistance of rainbow trout (Oncorhynchus mykiss) against Yersinia ruckeri

This investigation was aimed to determine the efficacy of coriander seed extract (Coriandrum sativum) on physiological responses, immunity and disease resistance of rainbow trout, Oncorhynchus mykiss for eight weeks. A total number of six hundred rainbow trout (62 ± 0.81 g) were divided into four feeding groups including 0 (control), 0.5%, 1% and 2% of coriander seed extract (CSE). In the present study, rainbow trout fed with 2% of CSE showed significantly higher values of specific growth rate (SGR), final weight (FW) and condition factor (CF) in comparison with control group after eight weeks (P < 0.05). Regarding hematological indices results, the 2% dosage of CSE showed the highest amount of hematocrit and hemoglobin compared to control group (P < 0.05). In addition, significant improvement of lysozyme and alternative complement activity, were observed in 2% of CSE treatment (P < 0.05). After eight weeks post-feeding, 30 fish from each treatment were challenged with Yersinia ruckeri for 14 days. The findings presented that fish fed with CES, especially 2% of CSE inclusion, improved survival rate of rainbow trout against Y. ruckeri; however, there were no significant differences among the fish in control and treatment groups at the end of the eight weeks feeding with coriander seed extract. The present study demonstrated, dietary incorporation of coriander extract can improve growth factors, immunological indices and resistance of rainbow trout (O. mykiss) against Y. ruckeri infection.

PHYTOCHEMICAL AND PHYSICOCHEMICAL ANALYSIS OF MICROWAVE-ASSISTED EXTRACTION CORIANDRUM SATIVUM L. LEAVES AND ITS BIOLOGICAL EVALUATION

Objective: The main objective of the present work is to carry out the phytochemical constituent present and physicochemical analysis of microwave-assisted extract (MAE) of Coriandrum sativum leaves and to investigate its antibacterial activity.&#x0D; Methods: The powdered samples of the leaves were extracted and analyzed for physicochemical properties. Extraction was carried out for C. sativum using different solvents and used for phytochemical investigation. Infrared (IR) spectrum and antibacterial activity of MAE were carried out.&#x0D; Results: The results show that the percentage extract is different in the different solvents and also differs in phytochemical constituents. Phytochemicals present in the MAE also confirmed by IR spectroscopy. MAE is active against bacteria.&#x0D; Conclusion: The phytochemicals present in C. sativum is confirmed by IR spectrum and these phytochemicals responsible for antibacterial activity.

Changes in tissue gadolinium biodistribution measured in an animal model exposed to four chelating agents.

This study investigated the potential to reduce gadolinium levels in rodents after repetitive IV Gadodiamide administration using several chelating agents. The following six groups of rats were studied. Group 1: Control; Group 2: Gadodiamide only; Group 3: Meso-2,3-Dimercaptosuccinic acid (DMSA) + Gadodiamide; Group 4: N-Acetyl-L-cysteine (NAC) + Gadodiamide; Group 5: Coriandrum sativum extract + Gadodiamide; and Group 6: Deferoxamine + Gadodiamide. Brain, kidney, and blood samples were evaluated via inductively coupled plasma mass spectrometry. The brain was also evaluated histologically. Kidney gadolinium levels in Groups 4 and 5 were approximately double that of Group 2 (p = 0.033 for each). There was almost no calcification in rat hippocampus for Group 4 rodents when compared with Groups 2, 3, 5 and 6. Our preliminary study shows that excretion to the kidney has a higher propensity in NAC and Coriandrum sativum groups. It may be possible to change the distribution of gadolinium by administrating several agents. NAC may lower Gadodiamide-induced mineralization in rat hippocampus.

Intake of Heated Leaf Extract of Coriandrum sativum Contributes to Resistance to Oxidative Stress via Decreases in Heavy Metal Concentrations in the Kidney.

Coriandrum sativum (coriander) is an annual herb of the Apiaceae family and has been used as a traditional remedy. Here we examined whether heated leaf extract of coriander decreases the concentrations of heavy metals in tissues. Male ddY mice were given a drinking water containing 0.25% of heated leaf extract of coriander for 8weeks. Eight weeks after the intake, the concentrations of zinc, iron, copper, arsenic, and cadmium were measured in the liver and kidney. The intake of coriander did not modify the concentrations of all heavy metals tested in the liver, but decreased the concentrations of iron, arsenic, and cadmium in the kidney. Because heavy metals can induce oxidative stress, the effect of coriander intake on hydrogen peroxide-induced oxidative stress was compared between slices from the kidney and liver. The slices were immersed in Ringer solution containing 100μM hydrogen peroxide and aminophenyl fluorescein (APF), a probe for detecting reactive oxygen species (ROS). APF fluorescence was markedly increased in the control kidney slices, while the increase was completely blocked in kidney slices from coriander intake group. In contrast, APF fluorescence was also markedly increased in the control liver slices, while the increase was not blocked by coriander intake. The present study indicates that intake of coriander leaf extract contributes to powerful resistance to oxidative stress in the kidney, probably via decreased concentrations in heavy metals. It is likely that decrease in arsenic concentration to the detection limit is a major factor for the resistance.

Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice

Lead toxicity is the vital issue in the developing countries that causes serious health hazards in animals and humans. Prophylactic effects of vitamin E and coriander (Coriandrum sativum) extract on lead-induced testicular damage in Swiss albino mice were investigated in the present study by gross and histological studies in five groups (control, lead intoxicated, vitamin E-treated, coriander-treated, vitamin E and coriander-treated). Treatment was done for 42 days. In the present study, weight and length of left and right testes were significantly (p

Cardioprotective Efficacy of Coriandrum sativum (L.) Seed Extract in Heart Failure Rats Through Modulation of Endothelin Receptors and Antioxidant Potential

The present study aimed to evaluate the therapeutic and prophylactic potential of Coriandrum sativum extract in isoproterenol-induced heart failure (HF) in Wistar rats. Two weeks after the isoproterenol administration, rats developed severe impairment in left ventricular functions, reduced baroreflex sensitivity, and significant alteration in hemodynamic parameters and lipid profile. HF rats also exhibited enhanced lipid peroxidation and increased expression of endothelin receptors (ETA and ETB). Therapeutic and prophylactic treatment with C. sativum extract significantly (p < .05) improved the left ventricular functions and hemodynamic parameters and increased baroreflex sensitivity. It also inhibited lipid peroxidation, improved lipid profile, and downregulated the expression of endothelin receptors. Simvastatin treatment showed a similar cardioprotective effect. Our results suggest that C. sativum extract provides significant protection from heart failure possibly due to its ability to improve left ventricular functions and baroreflex sensitivity, attenuate lipid peroxidation, and modulate the expression of endothelin receptors.

Sensitivity of Coriandrum sativum extract on bacterial pathogens isolated from digestive system of rabbits, and its role on in vitro cecal gas production and fermentation

The present context was aimed to investigate the antibacterial potency of aqueous extract of coriander (Coriandrum sativum L.) leaves against bacterial pathogens isolated from the organs associated with digestive system of rabbit. This study also evaluated the influence of varied doses of aqueous extract of C. sativum (AECS) leaves on in vitro gas production (GP), methane (CH4) production, and some other pivotal fermentation parameters from caecal sample of rabbits. The pathogenic bacteria were isolated from mouth, caecum, and anus of rabbits, and further identified through morphological, biochemical, and molecular tools. The growth inhibitory characteristics of AECS against pathogens were determined using disc diffusion assay. Surprisingly, the result revealed lack of antibacterial potential at tested concentrations. Further, in order to demonstrate the in vitro GP and fermentation parameters in rabbits, four treatments comprising of 0, 0.6, 1.2, and 1.8 mL extract/g dry matter (DM) of AECS were used. Results showed no linear or quadratic effect (P > 0.05) on in vitro GP and CH4 production after the supplementation of AECS in the feeding diet. However, the inclusion of AECS at the concentration of 1.8 mL/g DM exhibited the lowest asymptotic CH4 production and initial delay prior to CH4 production. Similarly, the addition of AECS at 1.8 mL/g DM concentration reduced asymptotic GP as well as CH4 production, and improved fermentation parameters of rabbits when compared with the control and other tested doses. In a nutshell, the tested doses of AECS showed lack of antibacterial trait against the pathogenic bacteria isolated from mouth, caecum, and anus of rabbits. Besides, the AECS exhibited the unique potentiality of reducing GP and improving diversified fermentation parameters in rabbits, thereby suggesting its plausible role as an alternative to commercially available growth promoters in livestock industries.

Effects of Coriandrum Sativum Extract and Simvastatin in Isopreterenol Induced Heart Failure in Rats

Abstract Heart failure is a syndrome, caused due to structural and functional cardiac abnormalities, characterized by changes in the hemodynamic and neurohumoral mechanisms. It is becoming a major health burden worldwide. More effective therapies are desperately needed. Coriandrum sativum (C. sativum), a traditional spice crop has been known to possess many biological and medicinal properties. The present study was designed to investigate the cardioprotective efficacy of C. sativum in rat model of isoproterenol induced heart failure. Heart failure was produced by injecting isoproterenol subcutaneously (85 mg/kg twice at an interval of 24 h). Oral efficacy of seed extract was assessed on hemodynamic profile, antioxidant enzyme activities, lipid peroxidation, lipid profile, atherogenic indices, mRNA and protein expression of endothelin receptors (ETA and ETB) and histopathology. Treatment of heart failure rats with C. sativum orally (1g/kg b.wt) improved the altered hemodynamics, restored the cardiac antioxidant enzymes armory, attenuated oxidative stress, improved lipid profile, lowered atherogenic indices, decreased the levels of ETA and ETB receptor mRNA and protein, and restored the cardiac morphology. In conclusion, our results suggest C. sativum to be a cardioprotective agent in heart failure, possibly by the virtue of its ability to alleviate oxidative stress, improve lipid profile and endothelial dysfunction.

Anti-Nematodal Efficacy of Ivermectin (Oral) and Extracts of Coriandrum sativum in Sheep in Barani Livestock Production Research Institute (BLPRI) Kherimurat, District Attak Punjab Pakistan

Anthelmentic resistance (AR) is one of the major problems of livestock industry in developing countries. Using indigenous medicinal plants may be an alternate way to overcome this problem. Total 60 sheep nematode positive were selected and divided into 6 groups having 10 sheep each. Group A as un-treated control, group B was given Ivermectin at the dose rate of 0.2mg/kg body weight Group C and Group D was given the Chloroformic extract while Group E and F was given the Methalonic extract of Coriandrum sativum whole plant at the dose rate of 50 mg/kg and 100 mg/kg body weight respectively. After treatments, fecal eggs count reduction test (FECRT) (eggs/g) was carried out on day 7, 14 and 28. The EPG in untreated group at day 28 was increased 21.6%. Significant decrease in EPG was only observed at day 28 in Ivermectin treated group (92.6%) and Methanolic extract at a dose rate of 100 mg/kg body weight (60.69%). No significant results were recorded in remaining groups. It was concluded that 100 mg/kg body weight Methalonic extract of Coriandrum sativum would be a best alternative as anti-nematodal drug.

EVALUATION OF ANALGESIC ACTIVITY OF MURRAYA KOENIGII AND CORIANDRUM SATIVUM LEAVES EXTRACT IN ANIMAL MODEL.

Objective: The objective of the study was to investigate the analgesic activity of hydroalcoholic extract of Murraya koenigii and Coriandrum sativum leaves and compared it with standard drug in an animal model.Methods: Hydroalcoholic extracts of M. koenigii and C. sativum leaves were obtained using Soxhlet apparatus. The central analgesic property was screened by hot plate method in mice and tail flick method in rats. The pain reaction time (PRT) was measured at 30, 60, and 120 min. The peripheral analgesic activity was evaluated by acetic acid induced writhing in mice.Results: In hot plate method M. koenigii leaves extract at both doses and tramadol showed significant increase in PRT at 30, 60, and 120 min compared with control group. C. sativum leaves extract showed significant increase in PRT only at 60 and 120 min compared to control group. In tail flick method M. koenigii leaves extract at both doses, higher dose of C. sativum leaves extract and tramadol showed significant increase in PRT at 30, 60, and 120 min compared with control group. Higher dose of M. koenigii leaves extract (200 mg/kg) was comparable with standard drug tramadol in both the methods. M. koenigii leaves extract at both dose showed significant reduction in the number of writhing but C. sativum leaves extract failed to show any significant reduction in the number of writhing compared with control. Higher dose of M. koenigii leaves extract was comparable with standard drug tramadol.Conclusion: M. koenigii leaves extract showed both peripheral and central analgesic effect while C. sativum leaves extract showed only peripheral analgesic effect.

EVALUATION OF ANALGESIC ACTIVITY OF MURRAYA KOENIGII AND CORIANDRUM SATIVUM LEAVES EXTRACT IN ANIMAL MODEL.

Objective: The objective of the study was to investigate the analgesic activity of hydroalcoholic extract of Murraya koenigii and Coriandrum sativum leaves and compared it with standard drug in an animal model.Methods: Hydroalcoholic extracts of M. koenigii and C. sativum leaves were obtained using Soxhlet apparatus. The central analgesic property was screened by hot plate method in mice and tail flick method in rats. The pain reaction time (PRT) was measured at 30, 60, and 120 min. The peripheral analgesic activity was evaluated by acetic acid induced writhing in mice.Results: In hot plate method M. koenigii leaves extract at both doses and tramadol showed significant increase in PRT at 30, 60, and 120 min compared with control group. C. sativum leaves extract showed significant increase in PRT only at 60 and 120 min compared to control group. In tail flick method M. koenigii leaves extract at both doses, higher dose of C. sativum leaves extract and tramadol showed significant increase in PRT at 30, 60, and 120 min compared with control group. Higher dose of M. koenigii leaves extract (200 mg/kg) was comparable with standard drug tramadol in both the methods. M. koenigii leaves extract at both dose showed significant reduction in the number of writhing but C. sativum leaves extract failed to show any significant reduction in the number of writhing compared with control. Higher dose of M. koenigii leaves extract was comparable with standard drug tramadol.Conclusion: M. koenigii leaves extract showed both peripheral and central analgesic effect while C. sativum leaves extract showed only peripheral analgesic effect.

An allied approach for in vitro modulation of aldose reductase, sorbitol accumulation and advanced glycation end products by flavonoid rich extract of Coriandrum sativum L. seeds.

Traditional herbal medicines are attaining more popularity and are being widely practiced. Coriandrum sativum L. is one of the oldest herbal medicinal plants valued for its nutritional and medicinal properties. Present investigation was focussed on evaluation of attenuating potential of flavonoid rich extract of C. sativum (FCS) seeds against pathogenic markers of diabetic complications i.e. advanced glycation end products (AGEs), sorbitol and aldose reductase (ALR); by using in-vitro methods. Gas chromatography-mass spectrometry (GC-MS) and Infrared spectroscopy of FCS revealed the presence of different flavonoids. Results demonstrated that FCS has produced 79.80% inhibition of AGEs formation. Additionally, FCS was effective against sorbitol accumulation and ALR inhibition with IC50 values of 221 μg/ml and 6.08 μg/ml respectively. Molecular docking was conducted against three binding site for ALR, RAGEs and sorbitol dehydrogenase to explore their binding interactions with identified flavonoids. The constituents F2, F4 and F6 have shown good binding interactions with all the receptors. The visualisation of the docked complexes revealed the occurrence of hydrophobic forces and hydrogen bonding in receptor and docked constituents. The results were in support with experimental inhibitory activities of FCS against these biomarkers and provide a considerable basis for the identification and development of new inhibitors.

The Protective Effects of Hydro-Alcoholic Extract of Coriandrum sativum in Rats with Experimental Iron-Overload Condition

Background: Development of a new iron-chelating medication may eventually serve as treatment of iron overload diseases, such as thalassemia and other iron related disorders. Coriander sativum, a plant rich of phenol and flavonoids, has an iron-chelating effect in-vitro. The ethanolic extract of C. sativum was investigated for its in-vivo ameliorating effect on hepatic damage in rats with experimental iron overload. Methods: Twenty-four adult male wistar rats were randomly divided to 4 groups: healthy control, iron overload, iron overload treated with deferoxamine, and 200 mg/kg of C. sativum extract, respectively. Assessments were performed for serum iron, ferritin, and serum markers of hepatic and cardiac damages, antioxidant enzyme, and lipid peroxidation levels. Results: The results indicated serum iron and ferritin concentration were significantly increased in the iron overload group compared to healthy controls (P < 0.05). Treatment with the C. sativum extract significantly decreased iron and ferritin concentration (P < 0.05). Histopathological changes in the liver, kidney, and the cardiac system, and iron accumulation in the liver were observed in the iron overload group compared to healthy controls (P < 0.05). Treatment with C. sativum extract significantly decreased biochemical parameters, such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and creatine phosphokinase (CPK), and improved tissue damage and decreased iron accumulation in the liver compared to the iron overload group (P < 0.05). Conclusions: The study showed that the hydro-alcoholic extract of C. sativum has iron-chelating and liver protective effects similar to deferoxamine against experimental iron overload condition in rats.

To Evaluate the Antidiabetic and Rejuvenating Capability of Tissues on Alloxan Induced Diabetic Rats under the Effect of Ethanolic Leaf Extract of Coriandrum sativum: A Histopathological Study

Objective: To evaluate the antidiabetic and rejuvenating capability of tissues on alloxan induced diabetic rats under the effect of ethanolic leaf extract of Coriandrum sativum. Methods: Diabetic model was prepared by administration of alloxan monohydrate (150 mg/ kg i.p). The ethanolic leaf extracts of Coriandrum sativum at a dose of 200 and 400 mg/kg of body weight were administrated to diabetic induced groups for a period of 28 days. The effect of ethanolic leaf extract of Coriandrum sativum leaf extract on serum blood glucose, insulin, lipase, α- amylase and LDH as well as kidney function test [urea, uric acid, albumin, protein and creatinine] were measured in the alloxan induced diabetic rats. Results: In the acute toxicity study, ethanolic leaf extract of Coriandrum sativum leaf was non-toxic at 2 000 mg/kg in rats. The increased insulin level, albumin and protein level, decreased blood glucose and other biochemical parameters level were observed in diabetic rats treated with both doses of ethanol extract of Coriandrum sativum leaf compared to diabetic control rats. In Histopathological study were revealed toward normal. Conclusion: Ethanolic extract of Coriandrum sativum leaf possesses significant antidiabetic and rejuvenating capability of tissues.

Ameliorative effect of Yeast Extract, IAA and Green-synthesized Nano Zinc Oxide on the Growth of Cu-stressed Vicia faba Seedlings

T HE EFFECT of CuSO4 stress on some growth parameters and some metabolites in 14-day-old Vicia faba seedlings was studied. Irrigation with 150 mM CuSO4 resulted in remarkable reduction in root length, shoot height, fresh and dry weights of root and shoot, leaf area and photosynthetic pigments (Chl a, Chl b and carotenoids), while it raised Chl a/b ratio. Total soluble carbohydrates and protein contents were significantly depleted under Cu treatment. The ameliorative role of seed priming with different concentrations of yeast extract (25, 50, or 100 %), IAA (0.1, 0.3, 0.5 mM), or the green-synthesized Nano zinc oxide with average size 61 (nm) prepared from leaf extract of Coriandrum sativum (10, 50, 100 ppm) for different periods (6 and10h for yeast and IAA, 3 and 6h for Nano ZnO were studied. Results revealed that seed priming with 50% yeast or 0.1 mM IAA for 10h showed a remarkable increase in the measured growth criteria, chla, chl b, soluble carbohydrates and proteins but diminished chl a/b ratio compared with the Cu stress treatment. Seed priming with 100 ppm Nano ZnO for 6 h exhibited the most effective treatment in mitigating the harmful effects of Cu stress on growth criteria, Chla, Chl b, soluble carbohydrates and proteins.

FORMULATION AND IN VITRO EVALUATION OF POLY-HERBAL ANTI-AGEING FACE CREAM OF CORIANDRUM SATIVUM AND ROSE HIP OIL

Objective: The purpose of this study was to develop anti-ageing poly-herbal face cream by mixing the ethanol extract of C. sativum and rose hip oil in order to produce multipurpose effects on the skin such as fairness, softening and antiseptic effects.Methods: Ageing of the skin is the result of continuous "wear and tear" processes. Chronological skin aging is a universal and inevitable process, while in contrast, photoaging results from the UV rays of sunlight, and the damage becomes apparent in sun-exposed skin. Cream is defined as semisolid emulsions which may be oil-in-water (o/w) or water-in-oil (w/o) type and these semisolid emulsions are meant for external applications. In this study creams (o/w type) were formulated based on the anti-oxidant potential of herbal extracts and its evaluation. Coriandrum sativum plant extracted by using soxhlet method with water as solvent. The creams were formulated with coriander oil, rose hip oil with different concentrations namely F1 to F6.Results: The creams were to be stable during stability studies accordingly ICH guideline for 2 mo. Coriandrum sativum extract showed significant anti-oxidant activity with an IC50 value is 34.25 μg/ml, while for ascorbic acid the IC50 value was 46.68μg/ml.Conclusion: It can be concluded that herbal creams without side effects having antioxidant property can be used as provision of a barrier to protect the skin and avoid aging of the skin.