The Possibility of Using Zinc Oxide Nano Particles for Treatment of Bovine-Subclinical Mastitis, in Vitro Antibacterial Activity

Abstract

Bovine mastitis is an inflammation of mammary gland parenchyma in cows. It is caused by multiple pathogens that result in economic losses because of the reduction in milk production and poor quality of the milk. It is commonly caused by bacteria that multiply in the mammary gland and regional lymph nodes, damaging the mammary parenchyma. The present study focuses on the bovine mastitis treating capacity of zinc oxide nanoparticles by examining in vitro antibacterial activity, MIC and MBC against pathogens isolated and identified from sub-clinical mastitis in dairy cows. Zinc oxide nanoparticles were synthesized both chemically and green methods from a plant extract of Coriandrum sativum and characterized for phase and microstructural analysis. The milk sample from subclinical mastitis cows was collected from the veterinary hospital. The bacteriological examination revealed the presence of S. aureus and E. coli . The antibacterial activity of the zinc oxide nanoparticle was evaluated at 650, 850, 1300, 1700, 1950 and 2500 µg/ml concentrations and the minimum inhibitory concentration was found to be 650 µg/ml and 850 µg/ml for S.aureus and E.coli respectively, and minimum bactericidal concentration was 650 µg/ml and 1700 µg/ml for S. aureus and E. coli respectively. Keywords: Antibacterial activity, bovine subclinical Mastitis, Coriandrum sativum, chemical synthesis, green synthesis, minimum inhibitory concentration, minimum bactericidal concentration, zinc oxide nanoparticles DOI : 10.7176/CMR/11-10-01 Publication date: December 31 st 2019