Combination Of Heat Treatment Research Articles

Gradient residual stress evolution and its influence on fatigue life under combined carburising heat treatment and ultrasonic surface rolling process

This study combined carburising heat treatment (CHT) with the ultrasonic surface rolling process (USRP) to improve the rotary bending fatigue properties of 18CrNiMo7-6 alloy steel. Based on the experimentally obtained fatigue limit (σ-1), the evolution of gradient compressive residual stress (CRS) of CHT- and CHT + USRP-treated specimens was analysed below the fatigue limit stress level. Subsequently, the evolution mechanism of gradient CRS of the two reinforced specimens was discussed and analysed from the opposing effects of austenite transformation and dislocation density change on the evolution of CRS. The results demonstrated that the selective bending fatigue performance of the alloy was evidently improved after the introduction of CRS; however, the change in fatigue limit was not evident. The durability under other loads (such as cyclic tension/compression and torsion loads) needs further study. The gradient CRS evolution of CHT specimens was mainly caused by the large residual austenite transformation and small dislocation density change. Therefore, the volume expansion caused by residual austenite transformation gradually increased the gradient compression residual stress of CHT specimens with an increased number of cycles. Contrarily, the degree residual stress evolution of CHT + USRP specimens was more affected by the dislocation density, and the gradient compression residual stress gradually decreased with an increased number of cycles. Finally, the residual stress evolution rate of the two reinforced specimens was divided into two stages: fast and slow.

Разработка СВЧ-установки для термообработки отходов убоя животных

Every day, the Russian Federation processes 126.3 tons of animal slaughter waste into animal feed. With an installation capacity of 35 kg/h, the required quantity is 7000 units. As a rule, the devices are energy-intensive. The problem is to reduce the operating costs of heat treatment of raw materials while maintaining the feed quality. The article introduces a new installation powered by an electrically driven resonator with rationalized operating modes. The research featured mucous by-products that require thermal treatment to neutralize the smell and disinfect the raw material. The authors investigated the dynamics of heating and used the data obtained to develop a digital installation model and test the electrodynamics in the resonator. After that, they rationalized the operating modes, i.e., corona discharge, electric field, screen efficiency, generator power, installation performance, and energy costs. The complex action of electromagnetic radiation provided a continuous combined heat treatment, disinfection, and odor neutralization with reduced operating costs. The electrical resonator was coaxially located in the shielding and contained a coronating electrode, electric bactericidal UV gas discharge lamps, knives, screws, and emitters from air-cooled magnetrons. The annular volume between the resonator and the coat excited the traveling wave by electromagnetic radiation through the perforation. Its average perimeter was a multiple of half the wavelength. The heat treatment with disinfection and fat rendering required an intrinsic quality factor of 8000, a generator power of 4.4 kW, a productivity of 35–40 kg/h, and energy costs of 0.25–0.28 kWh/kg. When the electric field was 5 kV/cm and the corona discharge was 9.79 kV/cm, the corona of bactericidal lamps provided the required ozone concentration and the bacterial contamination fell down to an acceptable level. The new installation with an electrical resonator reduced operating costs for heat treatment of animal slaughter waste and maintained the high-quality of the resulting feed products.

Investigation the effect of surface treatment on the mechanical properties of coating

AbstractThe study analyzed surface treatment's impact on mechanical properties of Fe-based amorphous coatings. Specimens underwent six-hour treatments at 670 and 770 °C using vacuum heat. Results revealed distinct mechanical features in the coating: Vickers hardness reached 755, scanning electron microscope images displayed glassy phases, showcasing good wear resistance and compressive residual stresses at around −55 MPa. A remarkable 122% increase in compressive residual stress was noted through combined vacuum heat treatment and sandblasting. Volume wear decreased from the initial 18 to 14 mm3 after treatment at 670 °C followed by sandblasting, indicating a 30% enhancement in wear resistance. Yet, using vacuum heat treatment at 770 °C negatively impacted the coating's properties.

Effect of pH and Shear on Heat-Induced Changes in Milk Protein Concentrate Suspensions.

The effect of shear on heat-induced changes in milk protein concentrate suspensions was examined at different pH levels, revealing novel insights into micellar dissociation and protein aggregation dynamics. Milk protein concentrate suspensions, adjusted to pH of 6.1, 6.4, 6.8, or 7.5, underwent combined heat (90 °C for 5 min or 121 °C for 2.6 min) and shear (0, 100, or 1000 s-1) treatment. The fragmentation of protein aggregates induced by shear was evident in the control MPC suspensions at pH 6.8, irrespective of the temperature. At pH 7.5, shear increased the heat-induced micellar dissociation. This effect was particularly pronounced at 121 °C and 1000 s-1, resulting in reduced particle size and an elevated concentration of κ-casein (κ-CN) in the non-sedimentable phase. At pH 6.1 or 6.4, shear effects were dependent on sample pH, thereby modifying electrostatic interactions and the extent of whey protein association with the micelles. At pH 6.1, shear promoted heat-induced aggregation, evidenced by an increase in particle size and a significant decline in both whey proteins and caseins in the non-sedimentable phase. At pH 6.4, shear-induced fragmentation of aggregates was observed, prominently due to comparatively higher electrostatic repulsions and fewer protein interactions. The influence of shear on heat-induced changes was considerably impacted by initial pH.

Co-delivery mechanism of curcumin/catechin complex by modified soy protein isolate: Emphasizing structure, functionality, and intermolecular interaction

Modifying proteins to enhance their affinity for polyphenols is essential for developing a practical and efficient delivery system that promotes the synergistic effects of bioactive substances. The study investigated the nanoparticle complexation in both pure soy protein isolate (SPI) or SPI modified by ultrasonic combined heat treatment (UHSPI) with varying concentrations of curcumin/catechin (CU/CA) complex, and its effects on the functionality, structure and conformational changes of protein-polyphenol nanocomplexes. Additionally, the intermolecular interaction and co-delivery mechanisms of nanocomplexes were analyzed. Results showed that the physical modification process (28/40 kHz, 90 °C, 15 min) and a rational concentration of CU/CA (0.6 mg/mL) improve the encapsulation efficiency of polyphenols by SPI (up to 77% for curcumin, and 93% for catechin), resulting in a higher DPPH and ABTS scavenging capacity, along with the highest emulsification activity index, as well as enhanced storage stability and thermal stability. The interaction between UHSPI and CU/CA disrupted the protein's secondary structure and exposed more hydrophobic residues to a hydrophilic environment, and exhibited stronger fluorescence quenching. Molecular docking analysis revealed that protein-polyphenol interactions primarily relied on hydrogen bonds and hydrophobic interactions, and 11S subunits exhibited higher affinity with CU/CA. FTIR, fluorescence spectra, and XRD further confirmed the enhanced interaction ability between UHSPI and CU/CA, resulting in the formation of a more stable nanocomplex. Overall, the modified SPI effectively facilitates the co-delivery of an appropriate concentration of curcumin/catechin complex.

Unraveling the effect of combined heat and high-pressure homogenization treatment on the improvement of chickpea protein solubility from the perspectives of colloidal state change and structural characteristic modification

Chickpea protein (CP) is a promising plant protein ingredient, but the poor solubility has limited its broad application. In this study, heating followed by high-pressure homogenization (HPH) was used to improve the solubility of CP. The results showed that combined heat (80℃, 30 min) and HPH (80 MPa, 2 cycles) treatment exhibited an additive effect in improving the solubility of CP. This improvement could be attributed to the dissociation and the rearrangement of large insoluble protein aggregates into small-sized soluble protein aggregates, the increased exposure of hydrophobic residues and reactive sulfhydryl groups, the transformation of α-helices to β-sheets and β-turns. Moreover, the 11S subunits of CP could form reinforced disulfide covalent cross-links under heating + HPH, which may provide steric hindrance preventing the reassembly of large protein bodies. This work proposes an interesting approach to enhance the physicochemical properties of CP for tailoring techno-functional plant protein ingredients in food formulations.

Research Progress on Surface Damage and Protection Strategies of Armature-Rail Friction Pair Materials for Electromagnetic Rail Launch.

Electromagnetic rail launch technology has attracted increasing attention owing to its advantages in terms of range, firepower, and speed. However, due to electricity-magnetism-heat-force coupling, the surface of the armature-rail friction pair becomes severely damaged, which restricts the development of this technology. A series of studies have been conducted to reduce the damage of the armature-rail friction pair, including an analysis of the damage mechanism and protection strategies. In this study, various types of surface damage were classified into mechanical, electrical, and coupling damages according to their causes. This damage is caused by factors such as mechanical friction, mechanical impact, and electric erosion, either individually or in combination. Then, a detailed investigation of protection strategies for reducing damage is introduced, including material improvement through the use of novel combined deformation and heat treatment processes to achieve high strength and high conductivity, as well as surface treatment technologies such as structural coatings for wear resistance and functional coatings for ablation and melting resistance. Finally, future development prospects of armature-rail friction pair materials are discussed. This study provides a theoretical basis and directions for the development of high-performance materials for the armature-rail friction pair.

Heat-Induced Changes in κ-Carrageenan-Containing Chocolate-Flavoured Milk Protein Concentrate Suspensions under Controlled Shearing.

Milk protein dispersions containing added cocoa powder (1.5% (w/w)) and sucrose (7% (w/w)) and varying levels of κ-carrageenan (0.01, 0.03, or 0.05% w/w) were subjected to combined heat treatment (90 °C/5 min or 121 °C/2.6 min) and shear (100 or 1000 s-1) to investigate the heat stability of milk proteins. The application of shear led to a notable reduction in non-sedimentable proteins, resulting in an increase in the average particle size and apparent viscosity of the dispersions, particularly at high concentrations of k-carrageenan and elevated temperatures. This indicates that shear forces induced prominent protein aggregation, especially at higher κ-carrageenan concentrations. This aggregation was primarily attributed to the destabilisation of micelles and presence of loosely bound caseins within the κ-carrageenan network, which exhibited increased susceptibility to aggregation as collision frequencies increased due to shear.

Culture-dependent screening of endospore-forming clostridia in infant feces

BackgroundOnly a few studies dealt with the occurrence of endospore-forming clostridia in the microbiota of infants without obvious health complications.MethodsA methodology pipeline was developed to determine the occurrence of endospore formers in infant feces. Twenty-four fecal samples (FS) were collected from one infant in monthly intervals and were subjected to variable chemical and heat treatment in combination with culture-dependent analysis. Isolates were identified by MALDI-TOF mass spectrometry, 16S rRNA gene sequencing, and characterized with biochemical assays.ResultsMore than 800 isolates were obtained, and a total of 21 Eubacteriales taxa belonging to the Clostridiaceae, Lachnospiraceae, Oscillospiraceae, and Peptostreptococcaceae families were detected. Clostridium perfringens, C. paraputrificum, C. tertium, C. symbiosum, C. butyricum, and C. ramosum were the most frequently identified species compared to the rarely detected Enterocloster bolteae, C. baratii, and C. jeddahense. Furthermore, the methodology enabled the subsequent cultivation of less frequently detectable gut taxa such as Flavonifractor plautii, Intestinibacter bartlettii, Eisenbergiella tayi, and Eubacterium tenue. The isolates showed phenotypic variability regarding enzymatic activity, fermentation profiles, and butyrate production.ConclusionsTaken together, this approach suggests and challenges a cultivation-based pipeline that allows the investigation of the population of endospore formers in complex ecosystems such as the human gastrointestinal tract.

The Microstructural Refinement of the A356 Alloy Using Semi-Solid and Severe Plastic-Deformation Processing

Improving the engineering properties of A356 alloy is an appealing option for the automotive industry. This study aimed at refining and redistributing Si particles and the eutectic phase by applying T6 heat treatment to a semi-solid A356 alloy, followed by severe plastic deformation (SPD). Using a cooling-slope technique, the as-cast and rheocast samples were subjected to heat treatment prior to being processed using equal-channel angular pressing (ECAP) and high-pressure torsion (HPT) at room temperature. The results show that the brittle Si particles were effectively fragmented and redistributed in the homogenous microstructure. The grain size reduced from 170 μm to 23 μm in the as-cast sample after combining heat treatment and the cooling-slope. This was followed by the ECAP sample after six passes through route A (where the sample is not rotated between each pass), while it reduced to 160 nm after five turns of the HPT process in a heat-treated cooling-slope sample. The hardness of the heat-treated cooling-slope casting samples increased with the ECAP process; there was an increase from 61 HV to 134 HV for the as-cast alloy after six passes through route A. The hardness of the heat-treated cooling-slope sample improved with the HPT process to 211 HV after five turns.

Simulation optimization and experimental research on heat-treatment process for ring remanufacturing of TBM disc cutters

In the context of the double carbon target, this study aims to reduce the metal resource consumption caused by the replacement of the failed disc cutter rings of tunnel boring machine (hereafter referred to as cutter rings). For this purpose, for the first time, a cutter-ring remanufacturing method is introduced considering key heat-treatment processes of preheating and post-weld tempering through metal active gas arc welding. First, a finite element model was developed to simulate this remanufacturing process route, establishing the optimal parameter ranges for the preheating and tempering temperatures. Subsequently, a self-developed integrated experimental platform was designed combining heat treatment and surfacing remanufacturing. A three-factor and three-level orthogonal experiment was conducted within the optimized parameter ranges. Further, a weighted comparative and range analysis method is proposed, and the impacts of the critical heat-treatment processes on the remanufacturing performance of the cutter rings exhibiting a uniform wear are investigated, resulting in the identification of the optimal heat-treatment combination. Results revealed that these pivotal heat treatments are instrumental in curbing defects (e.g., cracks) and substantially decreasing the residual stress in remanufactured cutter rings. Furthermore, the post-weld tempering temperature is the primary factor influencing the comprehensive performance and an array of physical and mechanical properties, including indent depth, hardness, shear strength, and impact toughness. The comprehensive performance of the best-produced sample impressively surged by 10.5 % compared to a standard engineering cutter ring.

Investigation of the Effect of Combined Photo- and Thermal Annealing on the Structure and Properties of Zinc Oxide Films for Flexible Electronics

In this work, the evolution of the structural electrophysical parameters of nanostructured zinc oxide films, the preparation of a sol-gel by thermal treatment and combined treatment, including thermal exposure and UV exposure, were studied. It has been established that the use of thermal treatment of films leads to the formation of large aggregates (20—30 µm), density to enlargement with the duration of processing, while the structure of the material at a low level of the hierarchy, determined by the sensitivity of the sensitivity to analysis, as well as the optical band gap is practically independent from processing time. This is probably due to the formation of nanoparticles of the material responsible for the properties associated with the accumulation of the sol prior to its formation on the substrates. The introduction of additional UV irradiation into the system leads to the destruction of aggregates formed during heart failure and the formation of X-ray amorphous ZnO particles during treatment for 90 minutes. Increasing the processing time of the issuance to "crosslinking" the selection, the number of sizes of single crystals and the simultaneous increase in the concentration of point defects, leading to a decrease in the optical revealed band gap. Thus, the combined UV and heat treatment of the film makes it possible, on the one hand, to form a material with a nanocrystalline structure, and, on the other hand, to control its defectiveness. These results are promising for application in the manufacture of thermoelectric batteries, gas, catalysts and photocatalysts.

Combined CFD and Heat Treatment Simulation of High-Pressure Gas Quenching Process

Abstract To improve the process development for high pressure gas quenching a digital quenching simulation model combining the fields of computational fluid dynamics and heat treatment process simulation has been developed. It was found that the gas flow and hence the quenching properties depend on both local (geometry of parts, carrier and chamber) as well as global influencing factors (fan characteristics, system pressure and hydraulic resistances). Therefore, a computational fluid dynamics model that includes all these factors was realized. The approach includes a heat transfer analysis to determine the local heat exchange coefficients on a component level. By connecting the computational fluid dynamics model and heat treatment simulation the local quenching characteristics are used to compute the temperature history of the quenched part. Based on a thermo-metallurgical heat treatment simulation the computed local cooling curves and metallurgical phase compositions are used to accurately predict the part properties like microstructure and hardness. The applicability of the model has been confirmed by hardness measurements. Hardness results for different batch positions, batch setups or tray systems can now be computed enabling an efficient virtual development of the gas quenching process.

Investigation of combined surface treatments and coatings to increase the wear behavior of heat treatable steels

A combination treatment of nitriding and induction hardening can achieve high hardness depths in short treatment times and can therefore be seen as an alternative to deep nitriding. However, the compound layer, which makes a significant contribution to the wear resistance of nitrided components, decomposes during the induction heat treatment of the nitrided part. Therefore, the tribological behavior of parts processed this way is affected. In the present work, the wear behavior of surface layers resulting from combined heat treatment of EN42CrMo4 and PVD a-C:H(:Si) coating is investigated and evaluated. For the wear testing, a two-disk test rig was used. The wear was determined gravimetrically and by optical evaluation of the wear tracks. It was found, that the wear resistance of the investigated heat-treatable steel can be improved by duplex surface treatments such as nitriding and induction hardening as well as nitriding and PVD a-C:H(:Si) coating compared to the heat treated-only condition. By triplex treatment, no further improvement of the wear resistance could be observed.

Enhancing mechanical strength of electrospun nanofibers by coaxial electrospinning and thermal treatment

AbstractElectrospinning of nonwoven nanofibrous mats has received significant attention in recent years due to the high versatility and porosity of electrospun mats. However, electrospun mats generally suffer from low mechanical strength. This work reports a method to improve the mechanical strength of nanofiber mats by coaxial electrospinning combined with thermal treatment, without incurring significant dimensional shrinkage. Coaxial polyacrylonitrile (PAN)/polyvinylidene fluoride‐hexafluoropropylene (PVDF‐HFP) mats showed no shrinkage when tested at temperatures up to 240°C for 20 min, compared to a shrinkage of 94% for the homogenous PVDF‐HFP mats when treated at 190°C for 20 min. When treated at 178°C for up to 30 min, the coaxial fibres consistently showed changes in thickness of less than 10% and no significant change in area. The coaxial PAN/PVDF‐HFP mats showed negligible changes in average porosity after treatment at 178°C for 20 min. The mechanical strength of the coaxial samples heat treated at 178°C for 5 min was 7.72 MPa, a 22% increase from that before heating, and a 54% increase compared to the as‐spun homogenous PVDF‐HFP. Thus, the proposed technique of combining heat treatment with coaxial morphologies demonstrates significant potential for improving mechanical strength without dimensional shrinkage.

The effect of high-temperature heat treatment and homogenization on the microstructure of set yogurt curd networks.

Set yogurt's physical characteristics are greatly affected by the homogenization and heat treatment processes. In our previous study, set yogurt treated at 130°C and with the fat particle size reduced to ≤0.6 μm had equivalent curd strength, less syneresis and smoother texture than yogurt treated at 95°C. When investigating the mechanisms underlying yogurt's physical properties, it is important to evaluate the yogurt's microstructure. We conducted electron microscopy evaluations to investigate the mechanisms of changes in yogurt's physical properties caused by 130°C heat treatment and by a reduction in the fat globule size. We prepared yogurt mixtures by combining heat treatment at 95 and 130°C and homogenization pressure at 10 + 5 and 35 + 5 MPa and then fermented the mixtures in a common yogurt starter. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used for the structural observations. Fine particles were observed on the surface of the casein micelles of the yogurt treated at 95°C, and the coalescence density between micelles was high. The surface of the yogurt treated at 130°C had few fine particles, and the coalescence density between micelles was low. The yogurt treated at 130°C with 35 + 5 MPa homogenization had low coalescence density between casein micelles, but smaller-particle-size fat globules increased the network density. Approximately 30% of the fat globules were estimated to be incorporated into the yogurt networks compared to the volume of casein micelles. We speculate that 130°C heat treatment alters the structure of whey protein on the surface of casein micelles and interferes with network formation, but reducing the size of fat globules reinforces the network as a pseudoprotein.

Natural variation in Brachypodium distachyon responses to combined abiotic stresses.

The demand for agricultural production is becoming more challenging as climate change increases global temperature and the frequency of extreme weather events. This study examines the phenotypic variation of 149 accessions of Brachypodium distachyon under drought, heat, and the combination of stresses. Heat alone causes the largest amounts of tissue damage while the combination of stresses causes the largest decrease in biomass compared to other treatments. Notably, Bd21-0, the reference line for B. distachyon, did not have robust growth under stress conditions, especially the heat and combined drought and heat treatments. The climate of origin was significantly associated with B. distachyon responses to the assessed stress conditions. Additionally, a GWAS found loci associated with changes in plant height and the amount of damaged tissue under stress. Some of these SNPs were closely located to genes known to be involved in responses to abiotic stresses and point to potential causative loci in plant stress response. However, SNPs found to be significantly associated with a response to heat or drought individually are not also significantly associated with the combination of stresses. This, with the phenotypic data, suggests that the effects of these abiotic stresses are not simply additive, and the responses to the combined stresses differ from drought and heat alone.

Influence of Combined Heat Treatment and Hot Isostatic Pressure (HT-HIP) on Titanium Aluminide Processed by L-PBF.

Postprocessing is essential for improving titanium aluminide (TiAl) microstructure and part quality after using the laser powder bed fusion (L-PBF) method. It has been reported that Ti-48Al-2Cr-2Nb (%at) processed by L-PBF has internal defects and low fracture toughness. Microstructure control by heat treatment (HT) showed a significant improvement in the ductility of the material. Alternatively, hot isostatic pressing (HIPing) could be applied to reduce the residual stresses and internal defects formed during the L-PBF. Combining the benefits of these two subsequent processes into a single predetermined process is appealing for Ti-48Al-2Cr-2Nb (%at) to minimize cost. This work presents a novel strategy to postprocess L-PBF TiAl by applying combined heat treatment and hot isostatic pressing in one process, namely HT-HIP. The process includes three cycles with different conditions (i.e., temperature, time, and pressure). These conditions were determined to achieve improved part quality and microstructure. The results show that the tensile residual stresses decreased from a peak of 249 MPa in the as-built sample to compressive stresses that peaked at -90 MPa after the HT-HIP process. The number and size of internal defects could be greatly reduced. The defects were transformed into a regular spherical shape, which is good in terms of fatigue strength. Additionally, a duplex microstructure with lamellar α2/γ colonies could be introduced for better ductility. Different levels of duplex microstructure could be achieved along with the process cycles. The grain structure using EBSD analysis showed refined equiaxed grains, which demonstrate better strength after the HT-HIP process. Twinning boundaries were also observed in the HT-HIP sample. The grain orientation tendency to the build direction significantly reduced after the HT-HIP process. The nanoindentation test was applied to evaluate the nanohardness of the as-built and HT-HIP samples. It could be demonstrated that the nanohardness is dependent on the formed phases and lamellar density inside the grains. The mean hardness value was 8.19 GPa for the as-built sample, while it was 5.48 GPa for the HT-HIP sample.

Significant improvement in the strength-toughness and isotropy of laser powder bed fused Ti6Al4V alloy by combining heat treatment with subsequent laser shock peening

In this study, the influences of heat treatment (HT) in combination with subsequent laser shock peening (LSP) on the strength-toughness and isotropy of laser powder bed fused (LPBFed) Ti6Al4V alloy were investigated. Results suggested that the ultimate tensile strength (UTS) of the 0°, 45°, and 90° HT1-LSPed specimens were 1283 MPa, 1276 MPa, and 1273 MPa, respectively, with corresponding elongation (EL) of 20.5%, 19.6%, and 19.7%, respectively. Compared to these three LPBFed specimens, the UTS values increased by 14.3%, 20.0%, and 24.8% while the EL values increased by 133.0%, 117.8%, and 72.8%, respectively. Meantime, the differences in UTS and EL were significantly reduced in different build directions (BDs). The fracture modes of the three HT1-LSPed specimens changed from mixed fracture (brittle and ductile) to ductile fracture. Microstructural characterization indicated that HT transformed the originally needle-like α′ grains into stable α and β grains in the LPBFed Ti6Al4V alloy in different BDs, significantly reducing the anisotropy of the microstructures. The subsequent LSP induced deeper plastic deformation layers, generating favorable gradient CRS, gradient microstructures (high-density dislocation structures, nanocrystallines, and nanotwins), and gradient microhardness. HT1-LSP-induced CRS strengthening, microstructural strengthening (deformation strengthening, fine grain strengthening, and twinning strengthening), and microhardness strengthening contributed decisively to the significant improvement in strength-toughness and isotropy of the Ti6Al4V alloy fabricated by LPBF in different BDs.

Supramolecular structure modification of RuBisCO from alfalfa during removal of chloroplastic materials

The use of alfalfa as a dietary protein source is of great interest. Membrane filtration shows potential for removal of unwanted compounds in the purification of ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO), but fouling remains a challenge. It was hypothesized that treatments, namely centrifugation, ultracentrifugation, and heat clearing, prior to membrane processing modify the supramolecular assembly of the proteins, facilitating its filtration. The pre-treatments led to different aggregate structures. In particular, heating at 50 °C decreased the aggregate sizes by ∼500 folds relative to the raw juice, as measured by light scattering. The molar mass of RuBisCO decreased by ∼180 kDa after treatment with heat. Similarly, supernatants after ultracentrifugation also showed smaller structures. Unlike the other treatments, heating led to an efficient transmission of RuBisCO during microfiltration. These results demonstrate for the first time, the importance of controlling the supramolecular structure of RuBisCO when processing proteins from alfalfa juice. Industrial relevanceThe demand for more sustainable protein sources is continuously growing. RuBisCO is of particular interest because of its favorable amino acid composition. However, improved purification strategies are needed to extract it from green biomass. The results of this study serve as a proof-of-concept for the application of an industrially relevant process, mild heat treatment in combination with microfiltration, for the purification of RuBisCO from alfalfa green biomass.