The development of Additive Manufacturing (AM) has greatly facilitated the fabrication of cellular and lattice materials. Gyroid-based lattice structures, known for their distinctive properties such as interconnected porosity, high surface-to-volume ratio, and remarkable structural stiffness combined with specific energy absorption, have been extensively explored. Many studies examining the impact of design parameters on the mechanical properties of Gyroid lattice materials have utilized metal AM techniques. This research aims to evaluate the influence of two design parameters on the compressive properties of Gyroid structures obtained by fused filament fabrication (FFF). A full factorial analysis was employed to assess the effects of cell size and wall thickness on the compressive properties of polylactic acid (PLA) Gyroid lattices. Cell sizes were varied between 4 mm, 5 mm, and 10 mm, while wall thickness ranged from 0.4 mm, 0.6 mm, to 0.8 mm. After 3D printing, the print quality was assessed, samples were weighted and then subjected to compression testing. During compression, the lattices with 10 mm cells exhibited successive layer collapse, whereas the lattice with 4 mm and 5 mm cells displayed plastic deformation, marked by a plateau in the stress-strain curve. These behaviours were mostly independent of wall thickness, except for the 5 mm cell lattice with 0.4 mm wall thickness. The elastic modulus, yield stress and absorbed energy per volume aligned with the apparent density of the lattices, ranging between 1% and 12% of the bulk 3D printed material for both the stiffness and yield stress, and between 1% and 22% for the energy absorbed. Analysis of the fitted means indicated that doubling the cell size had a more significant impact on the measured properties than doubling the wall thickness, while doubling both the cell size and wall thickness exerted a more pronounced influence on the yield stress and strain. Notably, under the conditions of this study, the 3D printed PLA Gyroids behaved similarly to closed cell foams, despite their interconnected channels. Their compressive mechanical properties comparable to those of rigid polyurethane foams with closed cells.