Bio-organic Fertilizer Research Articles

Microbial community assembly in soil aggregates: A dynamic interplay of stochastic and deterministic processes

Understanding how distinct processes operate in mediating community assembly is a long-standing theme in (microbial) ecology. Particularly in soil microbial communities, we still lack a fundamental appreciation of how assembly processes structure communities at the fine-scale level of soil aggregates. In this study, samples from a long-term agricultural field subjected to different fertilization regimes were used to quantify the relative influences of stochastic and deterministic processes on soil bacterial community assembly. First, we found bacterial communities to be more phylogenetically clustered in larger soil aggregates comparatively to small aggregates (Spearman's r = −0.366, P < 0.05). Second, we found the overall relative influence of selection to gradually decrease with an increase of aggregate size (Mantel r = 0.161, P < 0.01). By partitioning aggregate sizes and fertilization regimes, we found that sites subjected to fertilization (including chemical, organic, and bio-organic fertilizers) displayed a stronger relaxation of selection and an increased influence of stochasticity with an increase in aggregate size; a pattern not significantly observed at the control (unfertilized) treatment. Collectively, our results highlight the importance of evaluating community assembly at the fine-scale levels of soil aggregates and illustrate how regional level disturbances (i.e., agricultural management) exert an influence on the dynamic interplay of stochastic and deterministic processes.

Importance of Bio-Organic Fertilizers on Peanut (Arachis Hypogaea L.) Nutrition Following Organic Farming Approach with Application of 15N Isotope Dilution Concept

Abstract Field trial was conducted under low fertile sand soil conditions to follow up the role of organic additives with presence and absence of compatible bacterial inoculum in enhancement of peanut growth and yield. Bradyrhizobium spp. (foreign strain USDA 3456), inoculation resulted in relative increase of seed yield, as average, by about 5% over the un-inoculated plants despite of organic additive source. Similarly, organic additives had increased the seed yield, in average, over the non-treated plant which relatively accounted for 5%, 16% and 37% as affected by animal manure, leuceana residues and quail feces, respectively. On overall means basis of N uptake by seeds, comparison between organic additives indicated the superiority of animal manure over both of leuceana residues and quail feces. Nitrogen derived from mineral fertilizer seems to be higher in absence of organic manure and tended to decrease with addition of different organic amendments. Accordingly, the portion of fertilizer-N remained in soil after harvest was, in general, very low especially under organic amended soil and in absence of bacterial inoculation. Nitrogen derived from air by different plant parts on the basis of overall means indicated higher records accounted for 45.22 kg ha-1 with treatment leuceana residues followed by quail feces (45.22 kg ha-1) and animal manure (32.89 kg ha-1), respectively but all of them were superior over the un-treated treatment. Portion of N derived from organic sources was higher in seeds and straw than roots and seed cover, respectively.

Transforming wet blue leather and potato peel into an eco-friendly bio-organic NPK fertilizer for intensifying crop productivity and retrieving value-added recyclable chromium salts

An attempt has been made to address two important issues, the solid waste management of leather industry and soil fertility. The SEM images revealed altered surface-morphology.The EDS elemental analysis exhibited presence of about 13.2% nitrogen (N), 50.56% carbon (C), 2.69% phosphorus (P) in the collagenous material of wet blue leather (WBL) after chromium removal. In potato peel biochar (PPB) prepared the EDS analysis corroborated the presence of N P K in 5%, 1.4% and 21.64% respectively. In the formulated bio-organic NPK fertilizer, using chromium free WBL and PPB, the percentage of N, P, K, was in 13.10, 2.41, 20.20% respectively which was authenticated by EDS. Its effect on okra (Abelmoschus esculentus) plant showed higher growth (1.11 fold fruit size) and total chlorophyll content (1.61 fold) than in untreated soil (control) but displayed similar result as in presence of chemical fertilizer. The released free ammonia in soil with bio-organic NPK was more (37.02%) than with chemical fertilizer (6.10%). DNA intercalation study showed the non-hazardous impact on soil. The FTIR, XRD, SEM-EDS, AAS further specified the conversion of the WBL extracted acidic chromium-rich solution by MgO into crystalline chromium for commercial use.

Decreased nitrous oxide emissions associated with functional microbial genes under bio-organic fertilizer application in vegetable fields

Bio-organic fertilizers enriched with plant growth-promoting microbes (PGPMs) have been widely used in crop fields to promote plant growth and maintain soil microbiome functions. However, their potential effects on N2O emissions are of increasing concern. In this study, an in situ measurement experiment was conducted to investigate the effect of organic fertilizer containing Trichoderma guizhouense (a plant growth-promoting fungus) on soil N2O emissions from a greenhouse vegetable field. The following four treatments were used: no fertilizer (control), chemical fertilizer (NPK), organic fertilizer derived from cattle manure (O), and organic fertilizer containing T guizhouense (O+T, referring to bio-organic fertilizer). The abundances of soil N cycling-related functional genes (amoA) from ammonium-oxidizing bacteria (AOB) and archaea (AOA), as well as nirS, nirK, and nosZ, were simultaneously determined using quantitative PCR (qPCR). Compared to the NPK plot, seasonal total N2O emissions decreased by 11.7% and 18.7% in the O and O+T plots, respectively, which was attributed to lower NH4+-N content and AOB amoA abundance in the O and O+T plots. The nosZ abundance was significantly greater in the O+T plot, whilst the AOB amoA abundance was significantly lower in the O+T plot than in the O plot. Relative to the organic fertilizer, bio-organic fertilizer application tended to decrease N2O emissions by 7.9% and enhanced vegetable yield, resulting in a significant decrease in yield-scaled N2O emissions. Overall, the results of this study suggested that, compared to organic and chemical fertilizers, bio-organic fertilizers containing PGPMs could benefit crop yield and mitigate N2O emissions in vegetable fields.

Distribution of trace elements in soil and plants under the action of bio-organic and mineral fertilizers

The deficiency of trace elements in the soil is often among the factors limiting the degree of realization of the genetic potential of crops. As a rule, the availability of trace elements to plants from soil is determined by their concentration in it, which is in the easily hydrolyzed phase. At the same time, some trace elements with increasing concentration in soil are considered as one of the most dangerous pollutants. After exceeding the established maximum permissible concentrations (MPC) and approximate permissible concentrations (APC), some trace elements are classified as heavy metals.

Combination of Organic Fertilizer, Anorganic Fertilizer and Bio-Fertilizer in Maize Cultivation in Dry Land, Banten

The research aims to study the response of the combination of organic fertilizers, inorganic fertilizers and bio-fertilizers to the growth and yield of Lamuru composite maize in the dry land of Kadumadang Village, Cimanuk, Pandeglang Regency, Banten. The treatments consisted of : A) 3 t / ha manure + urea 350 kg + 100 % fertilizer recommendation for Maize; B) Manure 3 tons / ha + 75% fertilizer recommendation for Maize; C) Manure 3 t / ha + 50% fertilizer recommendation for Maize and D) Organic fertilizer + 25% fertilizer recommendation for Maize. Biosilica application was carried out on the 14, 28, 42, and 56 days after planting (DAS) with a dose of 40 liters/ha. The results showed that the treatment of C that the combination of of fertilizer 3 tonnes/ha of manure + recommendation of 50% fertilizer recommendation for Maize and Biosilica gave the best growth and yield of corn, with shelled production dry corn of 6.2 t / ha (moisture content 17-18%). This result is 16% higher when compared to the productivity of Pandeglang Regency in 2019 which reached 5.3 t/ha.

Effects of bio-organic fertilizer on soil fertility, microbial community composition, and potato growth

Effects of bio-organic fertilizer on soil fertility, microbial community composition, and potato growth

Testing of bio-organic fertilizer based on organic waste to improve the productivity of vineyards

The authors developed an environmentally safe technology for obtaining liquid organic fertilizer. The technology includes obtaining compost by applying microflora containing actinomycetes and microscopic fungi, populating the compost with vermiculture Eisenia foetida and obtaining biohumus. In 2018-2020 fertilizers obtained from organic waste were used on industrial plantings of technical variety Bastardo Magarachsky (planting scheme: 3 × 1.5 m, formation - double-shouldered cordon, rootstock - Kober 5 BB, non-irrigated culture) as foliar dressing. Climatic zone and place of experiment: South Coast agroclimatic area, sub-area - with subtropical winter (annual rainfall - 450-500 mm; hydrothermal coefficient of moisture - 0.5-0.6). Standard methods of agronomic observations of growth and development of grape plants were used in the work. Consumption rate 2 l/100 liters of water. The number of treatments - six, the terms of application: shoots growth; before flowering; after flowering; berry growth; beginning of softening; beginning of sugar accumulation (a month before harvesting). It was found that at the background of the same potential productivity of grape plants compared to the control, the yield increased by 15,9%; mass fraction of sugars in berry juice - by 7,1%, mash output - by 14,7%; technological stock of coloring and phenolic substances - by 7,6%.

Effect of on farm produced organic bio enhancers on yield and quality of tuberose (Polianthes tuberosa L.) cv. Mexican Single under sodic condition

Effect of on farm produced organic bio enhancers on yield and quality of tuberose (Polianthes tuberosa L.) cv. Mexican Single under sodic condition

Effect of Plant Growth Promoting Rhizobacteria on Growth, Nutrient Uptake and Physiological Parameters in Sugar Beet under Different Watering Regimes

Two experiments were conducted in order to investigate the of nine ACC deaminase-containing, IAA-producing, N2-fixing and/or P-solubilizing bacteria, on the growth, yield, chlorophyll, macro- and micro-nutrient content, and selected morpho-physiological parameters of sugar beet, under five watering regimes. The experiment was set up in two sets; and the first set was harvested after 65 days and the second set was done after 130 days. Inoculation with multi-traits bacteria stimulated overall plant growth, including sugar content, root and leaf yield and the leaf chlorophyll contents, and macro- and micro-nutrient uptake, which might partly contribute to the activation of the processes involved in the alleviation of the effect of water stress. According to the results, at the level of 75, 50 and 25% of WHC under water constraint, beet yield parameters BF4, BF6, Bio-organic and mineral fetilizer was found effective. As an average of the five water regimes, bacterial formulations increased dry storage root weight by 6.5-27.7% and 9.1-27.3% and dry leaf weight by 6.1-26.7% and 3.9-25.8% at the first and second trials, whereas, mineral fertilizers (NP) and bio-organic fertilizers increased dry storage root weight by 24.5% and 9.3-15.5% and 20.2 and 9.2-15.2%, and dry leaf weight by 23.5% and 11.7-23.2% and 22.2% and 3.3-21.9%, respectively, compared with control. Moreover, water stress in sugar beet plants was alleviated partially by the inoculation with bacterial strains. Our results provide strong evidence that the role of bacteria in the performance of sugar beet plants in stressful environment of soils not only the improved plant growth, yield, and macro- and micro-nutrient content, but also the alleviation of water deficit and waterlogging stress.

POTENSI KONSORSIUM BAKTERI PEMACU PERTUMBUHAN SEBAGAI BAHAN AKTIF PUPUK ORGANIK HAYATI PADA TANAMAN JAGUNG

The purpose of this research are to determine the potential of consortium of plant growth-promoting bacteria as an active ingredients of bio-organic fertilizers and to determine the effect of bio-organic fertilizers on growth and production of maize. The research was carried out at the Politeknik Pertanian Negeri Payakumbuh Experimental Field, Limapuluh Kota Regency, West Sumatra in March-July 2020. The research was starting with the manufacture of water hyacinth bio-organic fertilizer (POH) which inoculated with a consortium of bacteria from the genera Pseudomonas and Bacillus. POH observations were carried out on species, population size, and nutrient content of bio-organic fertilizers. The POH application research was using a factorial design in a randomized block design with treatment I at POH 40, 80, 120, 160 ml / l water and treatment II with frequency of 2, 3, 4 times giving POH. The variables observed were N, P, K nutrient content, plant dry weight, and dry maize seed production. The results of the POH study contained P. aeruginosa, B. subtilis, and B. cereus with a total population of 2,8,107–2,8,108, potentially as active ingredients for bio-organic fertilizers. POH water hyacinth can increase plant N, P, K nutrients, plant dry weight, and dry maize seed production. The optimal POH dose for all observed variables is 80 ml / l of water.

Integrated use of bio-organic fertilizers for enhancing soil fertility–plant nutrition, germination status and initial growth of corn (Zea Mays L.)

Abstract The need for cheap and affordable alternate sources of plant nutrient inputs to boost the nutrient level of degraded arable farmlands has been a major concern for soil scientists, agronomists, and local farmers. In 2018, a greenhouse and laboratory pot experiment was carried out under control conditions for 21 weeks to evaluate the effect of plant growth-promoting rhizobacteria (Azospirillium brasilense), spent grain (SG) organic wastes from the beer industry, and chemical fertilizer (CF) on the soil fertility–plant nutrition, corn (Zea Mays L.) germination rate in calcareous soil. The treatments consisted of CK (control), SG (spent grain, 20 g kg−1 soil), Az (Azospirillium seed inoculation), CF (100% of NPK recommended fertilizers), bio-organic (Az with SG), bio-mineral (Az with CF), and organo-mineral (50% of SG +50% of CF). These treatments were arranged in a randomized complete block design and replicated three times. Results showed that after 10 and 21 weeks, SG application significantly increased soil organic carbon by 69% and 67.8%, total nitrogen (TN) by 87.66% and 85.44%, and raised available P (P) by 75.7% and 87.23%, respectively. The final germination percentage (FGP), germination index (GI), coefficient of velocity of germination (CVG) and plant height were significantly higher in the amended pots than that of the control pots after 21 days of planting. The SG with Az treatments had the highest GI, CVG, and FGP of 9.23, 0.19 day−1, and 100 %, respectively, while the least values were obtained in the CF 2.37, 0.12 day−1, and 61.6%, respectively. The highest values of N/P ratio, available Fe2＋, Zn 2 + , and Mn 2 + were 401, 20.35 mg kg−1, 7.27 mg kg−1, and 16.58 mg kg−1 respectively, as well as bio-fertilizers enhanced the dehydrogenase and urease enzymes by 1117.3 μ g TPF ml−1, and 275.4 mg NH 3 − Hg−1h−1 respectively, after 21 weeks of seed sowing. The final results among all treatments were in the order of SG > Bio-organic > Az > Organo-mineral > Bio-mineral > CF > CK. In conclusion, the application of SG and Az is recommended to improve TN and P use efficiencies, micro-nutrients uptake, plant seed germination performance of maize, and enhancement of calcareous soil properties.

RETRACTED: Soil Microbiome Manipulation Gives New Insights in Plant Disease-Suppressive Soils from the Perspective of a Circular Economy: A Critical Review

This review pays attention to the newest insights on the soil microbiome in plant disease-suppressive soil (DSS) for sustainable plant health management from the perspective of a circular economy that provides beneficial microbiota by recycling agro-wastes into the soil. In order to increase suppression of soil-borne plant pathogens, the main goal of this paper is to critically discuss and compare the potential use of reshaped soil microbiomes by assembling different agricultural practices such as crop selection; land use and conservative agriculture; crop rotation, diversification, intercropping and cover cropping; compost and chitosan application; and soil pre-fumigation combined with organic amendments and bio-organic fertilizers. This review is seen mostly as a comprehensive understanding of the main findings regarding DSS, starting from the oldest concepts to the newest challenges, based on the assumption that sustainability for soil quality and plant health is increasingly viable and supported by microbiome-assisted strategies based on the next-generation sequencing (NGS) methods that characterize in depth the soil bacterial and fungal communities. This approach, together with the virtuous reuse of agro-wastes to produce in situ green composts and organic bio-fertilizers, is the best way to design new sustainable cropping systems in a circular economy system. The current knowledge on soil-borne pathogens and soil microbiota is summarized. How microbiota determine soil suppression and what NGS strategies are available to understand soil microbiomes in DSS are presented. Disturbance of soil microbiota based on combined agricultural practices is deeply considered. Sustainable soil microbiome management by recycling in situ agro-wastes is presented. Afterwards, how the resulting new insights can drive the progress in sustainable microbiome-based disease management is discussed.

Effects of lime and ammonium carbonate fumigation coupled with bio-organic fertilizer application on banana fusarium wilt and bacterial community.

Taking banana continuous planting soil with high banana fusarium wilt disease incidence as a test site, we examined the effect of lime and ammonium carbonate fumigation coupled with bio-organic fertilizer on the suppression of banana fusarium wilt disease and the structure and composition of bacterial community, using real-time quantitative PCR and high-throughput sequencing. The results showed that the disease incidence was reduced by 13.3% and 21.7% in the treatments of LAOF (lime and ammonium carbonate fumigation coupled with organic fertilizer) and LABF (lime and ammonium carbonate fumigation coupled with bio-organic fertilizer), respectively, compared with OF (application of organic fertilizer without fumigation), while the copy number of Fusarium was decreased by 22.4% and 33.0%, respectively. Compared with non-fumigation treatment, lime and ammonium fumigation coupled with different fertilizer applications significantly reduced bacteria richness and diversity, with different community structure, while fumigation had a decisive effect on bacterial community composition. Bacterial richness and diversity of LABF were lower than those of other treatments, while microbial community structure was clearly disparate from other treatments. Compared with non-fumigation treatment, the relative abundance of Mizugakiibacter, Brucella, and Rhodanobacter were significantly improved in the fumigation coupled with different fertilization treatments. Those three genera in LABF were higher than those in LAOF, with significant differences for the relative abundances of Mizugakiibacter and Brucella. Therefore, fumigation combined with bio-organic fertilizer application could reduce the copy number of pathogen, alter soil bacterial community structure and stimulate beneficial bacteria in the resident soil, and thus reduce the occurrence of banana fusarium wilt.

Response of watermelon (Citrullus lanatus) to plant compost, kitchen wastes and chicken manure composted with PGPR microbes as Bio-Organic fertilizers

Field experiments in two different locations were conducted at the Faculty of Agriculture, (Saba-Basha) - Alexandria University, during summer season of 2018/2019. The main objective was to investigate the effectiveness of different combinations of compost types on yield and quality of watermelon (cv.Skata F1 hyprid). The study was conducted with randomized complete block design with three replicates. Seven treatments were carried out in this investigation i.e. (T1-Jojoba leaves compost, T2-Compost A,T3-Compost B, T4-Compost C, T5-Compost D, T6-Compost E and T7- Control treatment (R100% NPK). The effects of the treatments on the growth and yield of watermelon were determined. Application of different types of compost increased the vine length, number of leaves, number of branches, number of fruits/plant, average fruit weight, fruit length, fruit diameter, mineral contents of leaves and fruits and improved total fruit yield. The obtained results indicated generally that application of chicken manure (T6) produced the highest values of all yield and its components and chemical composition of watermelon plants during both locations compared with the inorganic treatment. Also,Jojoba leaves compost (T1) and compost B ( 100% Compost A + PGPR) gave the highest mean values of the chlorophyll content in leaves character. In addition to Compost C ( 50% Compost A + 50% Chicken manure) gave the best results in mineral content of leaves characters. The findings in this study strongly recommended that chicken manure, beneficial microbes (PGPR), plant composted residues and kitchen wastes would be advantage if the farmer's target is to maximize watermelon yield.

Effects of bio-organic and microbial fertilizers on growth and soil nutrients of Vaccinium spp. seedlings

Effects of bio-organic and microbial fertilizers on growth and soil nutrients of Vaccinium spp. seedlings

Evaluation of the Effects of New Environmental Additives Compared to Mineral Fertilizers on the Leaching Characteristics of Some Anions and Cations under Greenhouse Plant Growth of Saline-Sodic Soils

Aims: The aim of this study was to determine and monitor the influences of organic and biological additives compared to mineral fertilizers on leaching characteristics of anions and cations, also to know more about the ability of these additives to make complexes with dissolved and toxic salts to decrease soil salinity. Background: Salt-affected soils comprise of saline and sodic soils which differ in origin, physico-chemical properties and the constraints to plant growth. Due to the presence of excess soluble salts (e.g. sodium (Na+) and chlorides (Cl-)). Methods: Ten treatments were established, including two levels of spent grain (environmental organic wastes from the beer industry), S1(10 g of spent grain / kg soil) and S2 ( 20 g of spent grain / kg soil); two levels of compost M1(10 g of compost / kg soil) and M2 ( 20 g of compost / kg soil); mixed M1 with S1 (M1S1); inoculation of Azospirillum brasilense (A1); inoculation A1 with S2 (A1S1); inoculation A1 with M1 (A1M1); 20:20:20 of N, P, and K fertilizers (NPK), and control (CK, without any additives). All treatments were mixed with 30 kg soil pots under greenhouse conditions, corn (Zea mayz L.) seeds were sown in the soil pots. The most relevant nitrogen and salt in soil leachates were collected and analysed every 20 days for 100 days. The soil leachates were collected under plant growth from pots by closed system. Results: The result revealed that organic additives such as A1 and S2 treatments effectively decreased soil pH, soil EC, and reduced NaCl concentration in soil leachates. The Ca2+ and K+ cations in the soil leachates were not stable at high levels of organic additives. Soluble NO3- and NH4+ were significantly lower in A1, S2, and A1S1 treatments than in NPK, M2, and CK treatments. Soil treatment with A1 and S2 significantly improved the soil chemical environment by increasing the Cation Exchange Capacity (CEC) and soluble and exchangeable-K+ and thus limited entry of Na+ into the exchange complex in soil and consists complex with soluble Na+ as sodium humate form. Conclusion: In the final, the highest nitrogen use efficiency with the least NO3- and NH4+ losses in saline-sodic soil was also found in S2 and A1 treatments. Moreover, under this bio-organic fertilization way, NO3- concentrations in soil leachates was outside of danger of damaging the environment. Thus, spent grain with Azospirillum were suggested to be the optimal fertilizer with the lowest nitrogen leaching losses, best yield, quality, and the least groundwater environmental risk under corn (Zea mays L.) organic and bio-organic cultivation comparing with chemical cultivation.

The Soil Nutrient Environment Determines the Strategy by Which Bacillus velezensis HN03 Suppresses Fusarium wilt in Banana Plants.

Biological control agents (BCAs) are considered as one of the most important strategies for controlling Fusarium wilt, and bioorganic fertilizer, in particular, has been extensively investigated. However, little is known regarding how a biocontrol microorganism affects the suppression mechanisms when combined with different amendments. In this study, a pot experiment was performed using banana plants to investigate the different mechanisms by which the biocontrol bacterium Bacillus velezensis HN03 (isolated from our laboratory) and amendments suppress Fusarium wilt. The incidence of banana wilt was decreased under HN03 and was reduced further when HN03 was combined with compost, particularly wormcast. In the suppression of Fusarium wilt, HN03 was found to influence the soil environment in various ways. HN03 increased the peroxidase level, which improves plant defense, and was highest when combined with wormcast, being 69 times higher than when combined with cow dung compost. The high accumulation of Mg and P in the “HN03 + wormcast” and Zn and Mn in the “HN03 + cow dung” treatments was negatively correlated with disease incidence. Furthermore, HN03 re-established the microbial community destroyed by the pathogen and further increased the level of suppression in the wormcast. HN03 also enhanced the functional traits of the soil, including defensive mechanism-related traits, and these traits were further enhanced by the combination of HN03 + wormcast.

Sustained Effects of Remediation Materials on Soil Copper Remediation Under Oil-Rice Rotation

In this study, a continuous rape-rice rotation plot experiment was conducted over three years. Repair materials were continuously applied in the first two years, and no repair materials were applied in the second year. The repair effects of hydroxyapatite, lime, biochar, bio-organic fertilizer, and nano-materials on copper contaminated soil and the enrichment of copper in different parts of rape and rice were investigated. The results show that hydroxyapatite, lime, and nanomaterials can significantly increase soil pH, and different restoration materials can effectively inhibit the movement of soil copper. The effective copper treatment with lime restoration soil had the largest decrease. The four seasons of continuous application of restoration materials were 38.9%, 34.9%, 27.88%, and 29.04%, respectively, and the subsequent effect of lime passivation of effective copper was better than other restoration materials. The application of the repair material significantly reduced the copper content in edible parts of rape and rice. In the four seasons of application of the repair material, the maximum copper content in edible parts of different crops decreased by 46.03%, 22.2%, 29.44%, and 31.71%, respectively. Due to the application effect of the repair material, the copper content in the edible part of the two season crops, without the repair material, did not exceed the national food safety limit. With the use of different repair materials, the yields of rapeseed and rice were improved. This test can provide some theoretical basis and technical support for soil improvement in copper-contaminated areas.

Application of Water Hyacinth Liquid Bio-Organic Fertilizer Using Trichoderma Sp Bio-activator on Growth and Production of Green Mustard Plants (Brassica rapa var. Parachinensis)

Mustard greens (Brassica rapa var. Parachinensis) are known vegetables among consumers and have high economic value. Besides being used as a vegetable, mustard greens are also used for the treatment of various diseases so that they are included in the vegetable group which has an important role in meeting the needs of food, nutrition and medicine for the community. To increase the growth and production of green mustard plants, it is necessary to apply liquid bio-organic fertilizers that are sourced from organic materials with good nutrients needed by plants, one of which is water hyacinth weed. To improve the quality of water hyacinth liquid bio-organic fertilizer, it can be applied with the bio-activator Trichoderma sp which functions to produce liquid bio-organic fertilizer with high nutrients for plant growth and production. The purpose of this study was to determine the growth of mustard greens including plant height, number of leaves and leaf width and to test the production of mustard greens including caratenoids and vitamin C content. This study used a completely randomized design (CRD) with a single factor, namely fertilizer. Liquid organic (POC) water hyacinth fermented using Trichoderma sp. which consists of 5 levels of fertilization doses, namely A (0 ml / L), B (8 ml / L), C (16 ml / L), D (24 ml/L) and E (32 ml/L) with 5 repetitions. Based on the results of analysis of variance, it showed that the dosage treatment of water hyacinth bio-organic fertilizer with the bio-activator Trichoderma sp had a significant effect on p &lt;0.05 on plant height, leaf width and the number of green mustard leaves at 4 weeks of age 21 day after planting. with the results of the chemical content test which showed that treatment E (32 ml/L) contained Karetenoid (0,2596 mg/100g of material) and vitamin C (0.2784 mg/100 g of material). The best dose based on the results of the study was treatment E (32 ml/L) which had a higher average plant height, leaf width and number of leaves compared to other treatments and had high vitamin A and vitamin C content. To increase the growth and production of mustard greens and other horticultural cultivation processes, it is advisable to use liquid bio-organic fertilizer for water hyacinth with the bio-activator Thricoderma sp as an alternative to natural, environmentally friendly liquid organic fertilizer.