Bio-organic Fertilizer Research Articles

Економічна та біоенергетична ефективність використання дигестату біогазових станцій при вирощуванні сільськогосподарських та овочевих культур в умовах євроінтеграції України

The purpose of the article is to determine the economic and bioenergy efficiency of bioorganic fertilizer based on digestate, taking into account the cost of basic nutrient elements. The article presents calculations of economic indicators of the examined elements of the technology of growing corn for grain and vegetable crops in Ukraine, which indicate that the cultivation of these crops is economically beneficial in all versions. The study found that increasing the economic efficiency of corn production for grain and vegetable crops when applying different norms of fertilizer application is achieved due to the more significant positive impact of yield growth compared to additional costs associated with the use of these agritechnical means, while additional costs caused by the use of fertilizers pay off many times. It is proved that the use of mineral fertilizers and their combination with high norms of bioorganic fertilizer (digestate) for corn and vegetable crops contributes to an increase in yield, and the energy efficiency of this fertilizer is quite high, which indicates the energy saving concerning the studied technology of growing crops. It is advisable to apply such fertilizer norms at which it is possible to obtain the greatest energy output at optimal costs, ensuring compliance with the priority policy of energy saving in agricultural production. The theoretical and practical provisions on the ecological problem (utilization of livestock waste, in particular in the case of pig farms), the energy problem (biogas production in a biogas plant) and the agronomic problem (obtaining organic fertilizer) have been further developed, which ensures an increase in yield and improvement of the quality of agricultural and vegetable crops, that is, makes it possible to obtain high-quality crop and vegetable products during utilization of livestock waste. The proposed approach to the economic assessment of technologies for growing corn for grain and table root crops, depending on the fertilizer application system, makes it conceivable to increase the level of productivity of agricultural and vegetable crops, provided that bioorganic fertilizers are effectively used in modern conditions of a sharp increase in the cost of mineral fertilizers. At the same time, the energy efficiency of growing corn for grain and table root crops depending on the fertilizer system has been determined, which will increase crop yields in the absence of an increase in fertilizer costs.

Induced Bitter Gourd Yield Enhancement by Bio-Organic Fertilizer Application Associated with Rhizosphere Microflora Alteration

Induced Bitter Gourd Yield Enhancement by Bio-Organic Fertilizer Application Associated with Rhizosphere Microflora Alteration

Complex Responses of Soil Bacterial and its Metabolites to Bio-Organic Fertilizer in Soil-Dendrocalamus Farinosus Scenarios

Complex Responses of Soil Bacterial and its Metabolites to Bio-Organic Fertilizer in Soil-Dendrocalamus Farinosus Scenarios

Разработка биосубстрата как стимулятора роста для выращивания органических видов сельскохозяйственных культур

Environmental pollution, the energy intensity of the production of mineral fertilizers, and the consequences of the globalization of the economy have led to the search for alternative farming systems focused on the biologization of agriculture, the development of environmentally friendly technologies for the cultivation of agricultural crops based on the use of modern forms of organic and bacterial fertilizers. A comprehensive solution to the problem of maintaining the balance of nutrients in the soil is the use of bioorganic fertilizers that combine the ability to improve the mineral nutrition of plants, fixation of atmospheric nitrogen, as well as stimulate the growth and development of plants. In this paper, the growth-stimulating effect of biosubstrate on seeds of organic agricultural crops such as wheat, lentils, peas, and buckwheat is investigated. The object of research was a biosubstrate consisting of a biopreparation (Saccharomyces cerevisiae Z4 YRKM 0843, Lactobacillus casei 12/2C B-RKM 0844, Streptomyces collinus P1 Ac-RKM 0845) and cattle manure effluents. During laboratory research, the growth-stimulating effect of the biosubstrate on seed germination and their growth indicators was established. Namely, a 10% and 50% solution of the biosubstrate improved the germination and germination of seeds of agricultural plants more than a 25% solution. This biosubstrate can be recommended as an environmentally safe growth stimulator when growing organic types of agricultural plants. Thus, the use of bio-organic fertilizers based on growth-stimulating microorganisms in the cultivation of various crops reduces the chemical load on ecosystems and also leads to an increase in yields and the quality of environmentally friendly agricultural products.

Lignite Bioorganic Fertilizer Enhanced Microbial Community Stability, Plant‒Microbe Interactions, and Sunflower Growth in Saline-Sodic Soil

Lignite Bioorganic Fertilizer Enhanced Microbial Community Stability, Plant‒Microbe Interactions, and Sunflower Growth in Saline-Sodic Soil

Comparison of a novel combination of bio-organic fertilizers vis-à-vis a chemical fertilizer

Plant nutrients are essential for the production of healthy crops for the world’s expanding population and thus, they are a vital component of sustainable agriculture. Increased crop production depends on the type of fertilizers used to supplement essential nutrients for plants. The nutrient level fertilizers provide depends on their nature- each type of fertilizer has its advantages and disadvantages concerning crop growth and soil fertility. The management of using fertilizer must aim to ensure both an enhanced and safeguarded environment. Thus, a balanced fertilization strategy must be implemented. An experiment was conducted under field conditions to assess the effects of combinations of bio-fertilizers on agronomic and quality criteria of Brassica juncea (brown mustard), Basella alba (climbing spinach), and Amaranthus dubius (red spinach). Randomized block design with three replicas were used for the study, one set with the application of fertilizers containing Azotobacter, Rhizobium, Sesbania, Bacillus subtilis, Bacillus cereus, Bacillus megaterium, Pseudomonas fluorescens and Glomus (Mycorrhizal inoculant)- under bio-fertilizer; another with a mixture of urea, Potassium Nitrate, Super Phosphate, Potassium Sulfate, and Maple EM solution as chemical fertilizer and a control (water). Results indicated that yield and other plant criteria like chlorophyll content and gel volume were enhanced in bio-fertilizer treated plants compared to the plants grown with chemical fertilizer and control. In general, the application of bio-fertilizer significantly increased leaf length by 16-50%, the total number of leaves by 50-80%, plant size 19.15-63.15%, and gel volume by 147% (approximately) in comparison with untreated plants.

Effect of Mephosphonee on methane generation in organic waste processing

The purpose of the study is to determine an effective technological method for the complete anaerobic processing of agricultural waste. It also aims to create and implement an effective technological method of sufficiently complete anaerobic processing of agricultural waste, including taking into account biologically active additives. A new technology for the disposal of organic agricultural waste and the influence of Mephosphone on the process of methane formation using a biogas unit at a processing facility developed at Kazan State Agrarian University is considered. The results of theoretical and experimental studies are presented, which made it possible to significantly reduce the utilization time, and reduce the cost of electricity and fuel. The experiments were carried out in a low-capacity biogas unit (LCBU) without air access in a heat-insulated reactor filled by 2/3. The results of numerical studies of the products of conversion of cattle manure are presented. Manure was kept in the reactor for 7 days. The use of Mephosphone in ultra-low concentrations (10-4) made it possible to obtain a useful product in the form of high-quality organic biofertilizer and biogas. The results of microbiological analysis of experiments in the test center of the Tatar Interregional Veterinary Laboratory are shown.

Effects of the lignite bioorganic fertilizer on greenhouse gas emissions and pathways of nitrogen and carbon cycling in saline-sodic farmlands at Northwest China

Mitigating climate change and improving food production are major challenges worldwide. Applications of lignite-based bioorganic products (or lignite-based fertilizer) can improve soil physicochemical properties and crop production in saline-sodic farmland. However, little is known about the effects of lignite bioorganic fertilizer (LBF) on greenhouse gas (GHG) emissions and climate change mitigation. Thus, a two-year field experiment was conducted in saline-sodic farmlands in the upper Yellow River basin, Northwest China. The field experiment comprised six treatments, including a negative control without any organic fertilizer (CK), a positive control amended with sheep manure (FYM), and four treatments amended with 1.5 (LBF1), 3 (LBF2), 4.5 (LBF3), and 7.5 t ha−1 (LBF4) LBF. The results showed that the LBF treatments decreased the emissions of CH4 and CO2 while increasing N2O emissions when the application rate was over 3 t ha−1. Additionally, FYM treatment acted as a CH4 source, while LBF2 and LBF3 treatments were both CH4 sinks. The LBF3 treatment had the highest net ecosystem carbon budget (NECB) and the lowest net ecosystem global warming potential (NGWP), which were 6.04 and 4.82 t ha−1 and -22.09 and −17.39 t ha−1 higher than those of the CK treatment in 2019 and 2020, respectively. Moreover, the net ecosystem economic budget (NEEB) of the LBF2 and LBF3 treatments was higher than that of the other treatments. Compared with the CK treatment, the FYM treatment increased the NCEB and decreased NGWP but it also decreased the NEEB. For nitrogen and carbon cycling, the LBF3 treatment increased almost all gene families involved in nitrogen cycling, except for nirA and hao, while the FYM treatment reduced the nirKS, norBC, nosZ, and hao gene families. The modules for carbohydrate metabolism and methanogenesis were also reduced by the LBF treatments. In conclusion, the LBF2 and LBF3 treatments had higher NECB, NGWP, and NEEB, indicating that the application of lignite bioorganic fertilizer at 3.0–4.5 t ha−1 is appropriate for climate change mitigation in saline-sodic farmlands in the upper Yellow River basin, Northwest China.

The Valorization of Biolignin from Esparto Grass (Stipa tenacissima L.) Produced by Green Process CIMV (Compagnie Industrielle de la Matière Végétale) for Fertilization of Algerian Degraded Soil: Impact on the Physicochemical and Biological Properties

This study proposes a new use for a paper industry waste material, lignin, in agriculture and agronomy as a fertilizer for arid soils, while following a strategy aiming to both increase the amount of organic matter in these soils and decrease the impact of pollution caused by industrial discharges that contain organic and/or inorganic pollutants generated by the paper industry. In fact, this method works to improve soil quality through a new carbon-rich bioorganic fertilizer (biolignin) that results from a green method called CIMV, a targeted depollution objective of the paper industry. Over the course of 180 days, we monitored the physicochemical and biological characteristics of degraded soils treated with three different biolignin treatments of 0 (D0), 2 (D1), and 4 (D2) g/kg. The humification was then evaluated by the equation E4/E6. A remarkable variation of the physicochemical and biological parameters was observed in D1 and D2: temperature 12–38 °C, humidity 9–29%, and pH 7.06–8.73. The C/N ratio decreased from 266 to 49. After 180 days, the improvement in soil carbon content for the three treatments D0, D1, and D2 was 14%, 19%, and 24%, respectively. A maximum bacterial biomass of 152 (CFU/g soil) was observed on the 30th day for D1. Maximum laccase activity for D2 was observed on the 120th day. D1 and D2 recorded a significant degree of humification compared to D0. The best indicator of humification E4/E6 was observed in D1, where the value reached 2.66 at the end of the treatment period. The D2 treatment showed a remarkable effect improving the fertility of the degraded soil, which confirms that biolignin is a good fertilizer.

Effects of Inorganic, Organic and Bio-Organic Fertilizer on Growth, Rhizosphere Soil Microflora and Soil Function Sustainability in Chrysanthemum Monoculture

The production of chrysanthemums is severely hampered by Fusarium wilt, which is exacerbated by monoculture. In this study, the role of inorganic plant nutrition fertilizer (IPN), organic fertilizer (OF) and bio-organic fertilizer (BOF) in avoiding monoculture-related production constraints was evaluated. We conducted a series of greenhouse experiments and studied the growth of chrysanthemum and changes in rhizosphere soil microflora and function. BOF application reduced the incidence of Fusarium wilt by 82.8% and increased the chrysanthemum shoot height and flower ray floret number by 31.4% and 26.1%, respectively. High-throughput Illumina HiSeq2500 sequencing results indicated that BOF and OF treatments increased the values of α-diversity indices of bacteria and fungi. In addition, significant alterations in microbe community structures were found in response to IPN, OF or BOF application. Among the major genera detected after BOF treatments, the levels of Fusarium and Glycomyces decreased while Cladosporium, arbuscular mycorrhizal and endophyte groups increased. In particular, the abundance of Mariniflexile had a positive relationship (R = 0.693, p < 0.05) with the incidence of Fusarium wilt, while Cladosporium showed a significant negative relationship (R = −0.586, p < 0.05). Interestingly, an analysis of microbiomes based on 16S rRNA sequences revealed that the functions of signal transduction, bacterial secretion system, oxidative phosphorylation and the metabolism of carbohydrate, nitrogen and amino acids all increased in both BOF and OF treatments. The results suggested that BOF could be effective for chrysanthemum monoculture soil restoration, potentially by altering the microbial community structures and functions, which affect the physiological and morphological attributes of chrysanthemum in monoculture.

Controlled fermentation of food industrial wastes to develop a bioorganic fertilizer by using experimental design methodology

A controlled fermentation was conducted to develop and design a bioorganic fertilizer based on three wastes generated by the food industry: olive mill wastewater (OMW), molasses and rice hulls by inoculation with selected strains of lactic acid bacteria (LAB), of the species “ Lactiplantibacillus plantarum” and “ Levilactobacillus brevis ” and of the yeast of the species “ candida famata ”. The fermentation with commercial yeasts and particularly the species of “ saccharomyces ” produced an unstable fertilizer with a high pH value (6.14).Whereas, the fermentation test with an inoculum composed of 20% yeast and 80% lactic acid bacteria showed a decrease in pH at 4.66 and a maximum enzymatic activity of 43.900 IU/l after 30 h. This combination allowed an appropriate morphological and microbiological quality of the bioorganic fertilizer. To determine with precision, the optimum conditions of this fermentation an optimization design was applied. As a result of experiments and statistical validation of the fitted model, the bioorganic fertilizer with the lowest pH value was achieved after fermentation of 53.2% OMW, 19.1% molasses, 21.3% rice hulls and 6.4% inoculum content. The final pH was able to reach a value of 4.07 with desirability of the order of 99.7%. A fertilizer with the lowest possible pH allowed farmers longer storage periods and required no special storage conditions.

Assessing the effects of bio-organic and chemical fertilizers application on absorption of macronutrients and heavy metals in vegetables (Case study: Lepidium sativum)

Assessing the effects of bio-organic and chemical fertilizers application on absorption of macronutrients and heavy metals in vegetables (Case study: Lepidium sativum)

Organic amendments alleviate early defoliation and increase fruit yield by altering assembly patterns and of microbial communities and enzymatic activities in sandy pear (Pyrus pyrifolia)

Severe early defoliation has become an important factor restricting the development of the pear industry in southern China. However, the assembly patterns of microbial communities and their functional activities in response to the application of bioorganic fertilizer (BIO) or humic acid (HA) in southern China’s pear orchards remain poorly understood, particularly the impact on the early defoliation of the trees. We conducted a 3-year field experiment (2017–2019) in an 18-year-old ‘Cuiguan’ pear orchard. Four fertilization schemes were tested: local custom fertilization as control (CK), CK plus HA (CK-HA), BIO, and BIO plus HA (BIO-HA). Results showed that BIO and BIO-HA application decreased the early defoliation rate by 50–60%, and increased pear yield by 40% compared with the CK and CK-HA treatments. The BIO and BIO-HA application significantly improved soil pH, available nutrient content, total enzyme activity and ecosystem multifunctionality, and also changed the structure of soil bacterial and fungal communities. The genus Acidothermus was positively correlated with the early defoliation rate, while the genus Rhodanobacter was negatively correlated. Additionally, random forest models revealed that the early defoliation rate could be best explained by soil pH, ammonium content, available phosphorus, and total enzyme activity. In conclusion, application of BIO or BIO mixed with HA could have assembled distinct microbial communities and increased total enzyme activity, leading to significant improvement of soil physicochemical traits. The increased availability of soil nutrient thus changed leaf nutrient concentrations and alleviated the early defoliation rate of pear trees in acid red soil in southern China.

Effects of Chemical Fertilizer Combined with Organic Fertilizer Application on Soil Properties, Citrus Growth Physiology, and Yield

Chemical fertilizer has been excessively used for high yield of citrus around the world, especially in China; meanwhile, it deteriorates the citrus orchard soil environment. To resolve the conflict, the use of organic fertilizer provides a promising solution. However, the data about organic fertilizer used in citrus orchard is rarely available. Here, four treatments including CK (no fertilizer), CF (chemical fertilizer), OF + CF (chemical fertilizer reduction combined with organic fertilizer; application of N, P2O5, K2O fertilizer and organic fertilizer is 0.564, 0.236, 0.336 and 10 kg/plant), and BF + CF (chemical fertilizer reduction combined with bioorganic fertilizer; application of N, P2O5, K2O fertilizer and bioorganic fertilizer is 0.508, 0.320, 0.310 and 10 kg/plant) were performed in a ‘Ponkan’ (Citrus reticulata Blanco) orchard to evaluate the effect of organic fertilizer on citrus yield, growth, soil properties etc. when nutrients of fertilizer of each treatment were equal except CK. The data obtained in 2019 and 2020 showed that both OF + CF and BF + CF were beneficial to improve soil fertility (soil physicochemical and microbe properties) and citrus growth physiology (growth, nutrient and photosynthesis), alleviate NO3−-N leaching, and promote yields. Comprehensive evaluation indicated that BF + CF was more effective than OF + CF. Together, organic fertilizer has the potential to substitute partial chemical fertilizer with improvement in soil properties, growth physiology, and yield of citrus.

Impact of Organic Matter, Inorganic, and Biofertilizer Combination on Soybean Yield in Entisol Soil of Indonesia

The study aimed to investigate an effect of organic fertilizer applied to rice in the first planting season for unfertilized soybean as second crop followed by inorganic and biofertilizer applied in the third season on soybean growth and yield under the rice-soybean-soybean cropping pattern in 2016/2017. The main plot was organic: (1) without organic amendment, (2) 10 t·ha−1 of chicken manure, and (3) 10 t·ha−1 precomposted rice straw. The subplot was inorganic N and P fertilizers and commercial biofertilizer (consisting of Rhizobium, nitrogen-fixing bacteria, and P-solubilizing bacteria) applied at planting of soybeans in the second dry season: (1) control (no inorganic and biofertilizer), (2) 50 kg urea ha−1, (3) 100 kg SP36·ha−1, (4) 50 kg urea + 100 kg SP36 ha−1, (5) biofertilizer, (6) biofertilizer + 25 kg urea ha−1, (7) biofertilizer + 75 kg SP36 ha 1, and (8) biofertilizer + 25 kg urea + 75 kg SP36 ha−1. Soybean planted on the first dry season after rice harvested was not fertilized (untreated). The results showed that the chicken manure amendment increased grain yield of soybean in the second season, i.e., from 1.03 t·ha−1 (without organic amendment) to 1.27 t·ha−1, an increase of 23%. There was no effect of rice straw on soybean grain yield. In the third season, however, the residual effect of straw compost or chicken manure increased soybean grain yield by 8% and 20%, respectively. Both straw compost and chicken manure also showed a positive effect on the use of inorganic and biofertilizers in increasing soybean productivity.

Bio-Organic Mineral Fertilizer for Sustainable Agriculture: Current Trends and Future Perspectives

Chemical (synthetic) fertilizers used indiscriminately for improved production pose a major threat to long-term soil fertility, the soil environment, and its components. The soil microbial community, however, plays a major and important role in fostering soil health and plant growth. While the use of synthetic fertilizers has a profound impact on plant growth, it also significantly alters the makeup of the microbial community towards a detrimental low, especially N and P fertilizers. Sustainable farming practices can reduce the depletion of natural resources and maintain both productivity and soil fertility. The use of minerals that contain fertilizer nutrients in their native state is a very promising approach to reducing emissions associated with the processing chemical industries. Organic material from natural sources (food waste, manure from livestock, agricultural biomass, etc.) acts as a source of microbial culture and encourages the release of nutrients into the soil during mineral weathering. The combination of nutrient-bearing minerals and their biological weathering agents together with organic matter has the potential to remediate, restore, and sustain depleted agricultural soils. Therefore, in this review, we emphasize the significance of sustaining agricultural productivity and microbial diversity in the rhizosphere, the two vital aspects of modern agricultural systems, through bio-organic mineral fertilizers.

ВЛИЯНИЕ ПРЕПАРАТА МЕФОСФОН НА ЭФФЕКТИВНОСТЬ ПРОЦЕССА ПОЛУЧЕНИЯ БИОГАЗА И УТИЛИЗАЦИИ УГЛЕРОДСОДЕРЖАЩИХ ОТХОДОВ

This article discusses a new method of utilizing carbon-containing waste into biogas with the addition of a biological active additive Mefosfon to the substrate in order to accelerate the process of obtaining biogas and preserve nutrients in the final product - organic fertilizer. The experiments were carried out in a small-volume biogas plant (MBU) without air access in a heat-insulated reactor filled to 2/3. The results of numerical studies of the conversion products of cattle manure are presented. The manure was kept in the reactor for 7 days. The use of the drug Mefosfon in ultra-low concentrations (10-4) made it possible to obtain a useful product in the form of high-quality organic biofertilizers and biogas. The conducted microbiological analysis of the experiments showed that in the experiment with the use of the drug Mefosfon, the content of enterococci and bacteria of the Escherichia coli group were lower than in the experiment without the drug Mefosfon. Bioconversion of the organic component of the substrate allows the use of waste in agriculture as organic fertilizers. Such processing can significantly reduce the dry weight of the treated organic waste and reduce the content of pathogenic microorganisms Salmonella spp. and Enterococcus spp., bacteriophage FX174, Ascarissuumova.
 Mefosfon reduces the time of maturation and neutralization of carbon-containing agricultural waste, which indicates the prospects of its use. After processing waste in a small-volume biogas plant, there are no pathogenic pathogenic microflora, helminth eggs, weed seeds, nitrites and nitrates. The final nitrogen content of the Mephosphon treated substrate is higher than that of the untreated substrate. The acidity values in the substrate pH 6.7 in the untreated and 7.5 in the treated substrate. Studies have shown that when the drug Mefosfon is added to the substrate, the release of biogas begins on 1-2 days and lasts up to 7 days. In this case, the biogas yield increases up to 25% compared to the use of the control substrate without the use of the Mefosfon preparation. The drug has found application in plant growing, animal husbandry, reclamation of contaminated soils

Effects of bio-organic fertilizers on soil organic carbon components and biomass of shamrock

Effects of bio-organic fertilizer to organic carbon (SOC), dissolved organic carbon (DOC), easy oxidation of organic carbon (ROC), microbial biomass carbon (MBC) and Shamrock biomass were studied. The results showed that the contents of SOC, DOC, ROC, MBC could be significantly increased by bioorganic fertilizer. When cultured for 180d, SOC and DOC increased by 42~154 and 9~40%, respectively, compared with T0 treatment. ROC and MBC content increased by 38~173 and 4~9%, respectively. In addition, with the increase of the application of bio-organic fertilizer, the SOC and DOC contents increased. In T4 treatment, the SOC and DOC contents were the highest, which were 25.05 g/kg and 74.89 mg/kg, respectively. In T3 treatment, the ROC and MBC contents were the highest, 9.84 g/kg and 167.09 mg/kg, respectively. Compared with the initial incubation period, SOC decreased by 6~17%, DOC increased by 3~13%, ROC and MBC increased by 15~64, 3~25%, respectively. The application of bio-organic fertilizer increased the aboveground and underground biomass and the highest value were in the T2 and T3 treatments, respectively. The application of biological organic fertilizer increased the content of active organic carbon components. Results of the present study provide evidence for the application of biological organic fertilizer in the agricultural area of China. Bangladesh J. Bot. 50(3): 911-916, 2021 (September) Special

Biological control of Streptomyces sp. UT4A49 to suppress tomato bacterial wilt disease and its metabolite profiling

ObjectivesEvaluation of Streptomyces isolated from Udhagamandalam, The Nilgiris, Tamilnadu, India for the biocontrol potential against the phytopathogen Ralstonia solanacearum and its metabolite profiling. MethodsStreptomyces strains were screened for plant growth promotion and antagonistic activity against R. solanacearum in in-vitro. The metabolite profiling of strain UT4A49 was studied through gas chromatography coupled with mass spectroscopy (GC–MS) analysis. Further, the identified lead compound 2,4-Di-tert-butylphenol (2,4-DTBP) was analyzed for its mechanism of action through in-silico docking study. The bioorganic fertilizer (BOF) amended potential Streptomyces strain UT4A49 was studied for biocontrol activity against R. solanacearum on tomato seedlings through pot culture experiments. ResultsThe Streptomyces strain UT4A49 showed the promising production of indole acetic acid (IAA) (76.0 ± 2.0 µg/ml), siderophore, ammonia, amylase, protease, cellulase as well as it solubilized the phosphate in-vitro. The strain UT4A49 showed strong in vitro antagonistic activity against R. solanacearum with the inhibition zone of 19.83 ± 0.44 mm in diameter. The isolated major compound, 2,4-Di-tert-butylphenol was showed strong inhibition against R. solanacearum. In silico docking study revealed that the 2,4-DTBP showed lower binding energy score with all targeted proteins which found better protein–ligand binding stability. In the pot experiment, biocontrol activity of BOF amended Streptomyces strain UT4A49 showed significant biocontrol efficacy was 78.5% against R. solanacearum in tomato seedlings. The strain UT4A49 was identified as Streptomyces sp. based on its morphological and molecular properties. ConclusionsThe detailed literature survey revealed that this is the first report on strong bioactive properties of 2,4-DTBP against R. solanacearum. This present study proved that bioorganic fertilizer amended with Streptomyces sp. UT4A49 is a promising biocontrol agent for tomato bacterial disease control.

Effect of Two Kinds of Fertilizers on Growth and Rhizosphere Soil Properties of Bayberry with Decline Disease.

Decline disease causes severe damage to bayberry. However, the cause of this disease remains unclear. Interestingly, our previous studies found that the disease severity is related with the level of soil fertilizer. This study aims to explore the effect and mechanism of compound fertilizer (CF) and bio-organic fertilizer (OF) in this disease by investigating the vegetative growth, fruit characters, soil property, rhizosphere microflora and metabolites. Results indicated that compared with the disease control, CF and OF exhibited differential effect in plant healthy and soil quality, together with the increase in relative abundance of Burkholderia and Mortierella, and the reduction in that of Rhizomicrobium and Acidibacter, Trichoderma, and Cladophialophora reduced. The relative abundance of Geminibasidium were increased by CF (251.79%) but reduced by OF (13.99%). In general, the composition of bacterial and fungal communities in rhizosphere soil was affected significantly at genus level by exchangeable calcium, available phosphorus, and exchangeable magnesium, while the former two variables had a greater influence in bacterial communities than fungal communities. Analysis of GC-MS metabonomics indicated that compared to the disease control, CF and OF significantly changed the contents of 31 and 45 metabolites, respectively, while both fertilizers changed C5-branched dibasic acid, galactose, and pyrimidine metabolic pathway. Furthermore, a significant correlation was observed at the phylum, order and genus levels between microbial groups and secondary metabolites of bayberry rhizosphere soil. In summary, the results provide a new way for rejuvenation of this diseased bayberry trees.