Application Of IBA Research Articles

Investigation of Rooting Performance of Some Grapevine Rootstocks with Aeroponic Method

In the study, the aeroponic system examined the rooting performances of three grapevine rootstocks (110R, 1103 Paulsen, 5BB) commonly used in Türkiye. IBA was applied to grapevine cuttings to encourage rooting. Grapevine cuttings taken during the winter dormancy period were placed in the aeroponic system after the necessary pretreatments. Significant improvements were observed in root length, root number, and rooting rate during the rooting process of cuttings placed in the aeroponic system. No diseases or pests were observed during the rooting period in the daily checks. As a result of the study, the highest root length was 17.77 cm in 110R rootstock, while the lowest was 7.1 cm in 1103P rootstock. The highest rooting rate was 76.67% in a 25 ppm IBA application in 1103P rootstock and 73.33% in a 100 ppm IBA application. The lowest rooting was 10% in 110R rootstock at 100 ppm IBA application dose and in the control group. The study's results revealed the positive effects of aeroponic systems in increasing rooting performance. The advantages offered by these systems are essential in terms of sustainability and efficiency in agriculture. Considering global challenges such as climate change and the reduction of agricultural areas, aeroponic systems are expected to find wider applications in the future.

Incision in the base and indolebutyric acid to promote the rooting of 'Bengal' litchi cuttings

A alternative to producing litchi (Litchi sinensis Sonn.) seedlings is by cuttings. However, the main drawback of using this method is related to the difficulty of rooting presented by this species. Thus, the objective of this study was to evaluate the rooting of semihardwood cuttings of litchi ‘Bengal’ at different concentrations of indolebutyric acid, with or without incision in the base. The experimental design was completely randomized with five replicates of 10 cuttings in a factorial 3X2 (three IBA concentrations: 0, 1.000 and 2.000 mg L-1; with or without incision in the base). After 126 days, the variables evaluated were: cutting survival, leaf retention, cutting with callus not rooted, rooted cutting, number of roots per cutting, length of major roots per cutting and dry weight of roots per cutting. For rooting, the IBA application was efficient only when the cuttings were not injured. In addition, there was no influence of the types of cuttings in the IBA concentrations. However, in those with incision at the base, the means were higher without the application of the plant growth regulator. Thus, the 'Bengal' litchi can be multiplied by semihardwood cuttings with incision at the base of the cuttings, without the use of IBA.

Morpho-physiological and phytohormonal changes during the induction of adventitious root development stimulated by exogenous IBA application in Magnolia biondii Pamp

Abstract Magnolia biondii Pamp is an important ornamental tree species widely grown and used as a rootstock in the propagation of different Magnolia varieties. In the current studies, anatomical, physiological and endogenous hormones were studied to check the effect of IBA 750 mg/L on the adventitious rooting and to provide theoretical and technical support for the propagation of Magnolia biondii Pamp through stem cuttings. Two thousand stem cuttings were prepared and divided into two groups i.e., IBA treated cuttings and water control. For the evaluation of antioxidant enzyme activities, and endogenous hormones levels, samples were collected on the day of planting and each 5th day and further steps were carried out in the laboratory according to the protocols and proper precautions. For the anatomical observations, samples were collected on the 13th, 15th, and 17th day for IBA treated cuttings while 21st, 23rd, and 25th day for control. Collected samples were preserved in the FAA solution and further observations were carried out in the laboratory. Anatomical observations showed that it took 13 days for the differentiation of root primordia to the appearance of young adventitious roots in IBA treated cuttings, while it took 21 days to develop primordia in the control. Antioxidant enzyme activities involved in ROS were significantly higher in the IBA treated cuttings compared to control. POD showed a peak on the 13th day before the emergence of roots in IBA treated cuttings while it showed a peak on the 21st day in the control. PPO showed a peak on the 21st day in the IBA treated cuttings while it showed a peak on the 29th day in the control. SOD showed a peak on the 17th day in IBA treated cuttings, while it showed a peak on the 25th day in the control. Exogenous application of IBA enhanced the endogenous IAA and GA3 levels compared to CK, while it reduced the levels of ABA continuously at the time of rooting and then increased gradually. Inclusively, our study suggests that IBA 750 mg/L is efficient for the rooting of Magnolia biondii Pamp cuttings, as it enhanced the process of antioxidant enzyme activities, endogenous hormones levels and reduced the time of root formation which is evident from the anatomical observations.

Effect of Indole-3-Butyric Acid (IBA) on Hardwood Cutting of Grapes (Vitis vinifera L.) cv. Pusa Navrang

Aims: The study aims to investigate the influence of different IBA concentration on the rooting and subsequent growth of hardwood cuttings of "Pusa Navrang" grapevine.
 Study Design: The study was performed in RBD design with replicate 5 times.
 Place and Duration of Study: The study was conducted at Research Farm, College of Horticulture, Mandsaur, during the period November 2020- March 21.
 Methodology: Polybags size of 5 X 7 inch was used for experiment. After the filling of growing media in poly bags, the hard wood cuttings of Pusa Navrang with uniform size having 4-5 functional bud was taken from one year matured canes from research farm, College of Horticulture, Mandsaur. The diameter of cutting about 0.75 to 1 cm (pencil thickness) were taken and before planting cuttings were treated with IBA solution by quick dip method.
 Results: The significant result were found for shoot parameter like shoot length, number of nodes per shoot, internodal length, number of buds per shoot, stem diameter, stem fresh weight, stem dry weight. Root Parameters like number of primary and secondary roots, root length, root thickness, whole root volume, fresh weight of root and dry weight of root. Leaf Parameters like number of leaves per plant, fresh weight of leaf, dry weight of leaf, leaf area, leaf area index, specific leaf weight, minimum days taken to emergence of 1st leaf and water content of leaf found in IBA @ 6000 ppm (G3).
 Conclusion: Among four concentration of growth regulator (IBA), the application of IBA @ 6000 ppm (G3) responded well in terms of rooting and shooting in hard wood cutting of grapes.

Unlocking the Potential: Impact of Plant Growth Regulators on Hybrid Rice Growth, Grain Yield and Harvest Index

Aim: This research focused on rice, especially in Asia, and examined how plant growth regulators, including hormones and synthetic compounds, affect crucial plant processes. The findings strongly support the use of foliar Indole-3-Acetic Acid (IAA) application to enhance hybrid rice growth, grain yield, and harvest index, promising advancements in agriculture.
 Study Design: Randomised Block Design with 11 treatments.
 Place and Duration of Study: The study, conducted at the Student Instructional Farm of C. S. Azad University of Agriculture & Technology in Kanpur, aimed to investigate the impact of foliar application of plant growth regulators on rice growth and yield during 2021 and 2022.
 Methodology: The experiment employed a rigorous randomized block design, testing various treatments involving the foliar application of IAA (25 & 50 ppm), IBA (25 & 50 ppm), NAA (25 & 50 ppm), Ascorbic Acid (50 & 100 ppm), and Kinetin (5 & 10 ppm). The profound effects of these different regulator concentrations were observed at key developmental stages, including tillering, anthesis, dough, and maturity.
 Results: Significant improvements in growth parameters, such as leaf and stem dry weights per plant, along with total leaf area per plant, were evident with the application of IAA at 50 ppm. Moreover, a notable increase in grain yield per plant and harvest index was observed at maturity, primarily with the foliar spray of IAA at 50 ppm, followed closely by IAA at 25 ppm. This consistent trend was also observed in yield-related attributes.
 Conclusion: The use of Indole-3-acetic acid (IAA) significantly impacted plant growth and yield, including leaf and stem dry weights, leaf area, grain yield, and harvest index, showing promise for enhancing hybrid rice production.

Application of BAP and IBA for In Vitro Callus Formation of Biduri (Calotropis gigantea)

A herbaceous plant called biduri (Calotropis gigantea) is useful as a medicine. The leaves, roots, and flowers are just a few of the organs that contain secondary metabolites. A callus is an accumulation of secondary metabolite-producing amorphous cells. Through the induction of plant growth regulators, callus formation can be induced. The goal of the study was to find the BAP and IBA concentration that would promote callus formation in biduri. The initialstem segments of biduri, which were 1.5 months old and 2 cm long, were used as explants. A factorial design using two components and three repetitions of each was employed in a fully randomized design. BAP concentrations (0, 1, 2, 3, 4 ppm) and IBA concentrations were the first and second factors, respectively (0, 1, 2, 3, 4 ppm). The callus induction time, callus morphology (color and texture), and callus appearance percentage were the factors that were observed. The results demonstrated that a green, compact callus could be produced at a concentration of 4 ppm BAP and 1 ppm IBA. The proportion of callus manifestation rose and the callus induction time decreased with an increase in BAP concentration of up to 4 ppm.

Promoting Rooting and Vegetative Growth in 41 B Vine Rootstock Cuttings with Encapsulated Nano Silver and IBA Applications

Globally, viticulture is carried on by grafting onto vine rootstocks obtained by hybridizing different vitis species or on their own roots due to their tolerance to biotic and abiotic stresses, especially phylloxera (Daktulosphaira vitifoliae). Sustainable regeneration of vineyard areas and new vineyard establishments require significant amounts of vine saplings each year. In addition to hormonal applications, nano products are also tested in the production of high quality vine nursery material. In this study, single nodal cuttings prepared from the vine rootstock 41 B (Vitis vinifera L. cv. Chasselas × Vitis berlandieri) in the resting period were used as plant material. The effects of encapsulated nano silver particles (AgNPs, 0.5 mg L-1 and 1 mg L-1), and IBA (0 and 1 g L-1) applications produced by green synthesis with cv. Öküz Gözü grape seed extract and AgNO3 on promoting rooting and vegetative growth in 41 B vine rootstock cuttings were evaluated in this study. AgNPs and IBA applications promoted rooting of cuttings, and vegetative growth of rooted cuttings. Applications increased shoot length, shoot diameter, number of nodes, fresh and dry weights of roots and shoots, and the highest value among these was achieved by the 1 mg L-1 AgNPs application. Leaf area and chlorophyll content (SPAD value) were determined by 1 g L-1 IBA application. AgNPs and IBA treatments resulted in increased rooting and vegetative growth compared to control. In conclusion, 1 mg L-1 AgNPs was recommended for vine propagation material and sapling producers as nano promotors for rooting and vegetative growth of 41 B rootstock cuttings.

Identification and Gene Expression Analysis of TIR1 in Easy and Hard to Root Olive (Olea europaea L.) Cultivars

Many plant species can be efficiently propagated vegetatively through the use of cuttings. Although simple to implement, the success rate of this method is restricted by the plants ability to produce adventitious roots. Auxin hormones are commonly used to induce adventitious roots in propagation by cuttings. The auxin metabolism influences plant growth and is significant because it can also affect the rooting performance of the IBA hormone commonly used during steel production. Olive trees (Olea europaea L.) are among the species that are commonly propagated using cuttings. However, the rooting success of olive cultivars grown for agriculture varies. For these reasons we analyzed the gene structure and expression rates of Olive TIR1, which is the receptor of auxins. We chose Gemlik (easy to root) and Domat (Hard to root) cultivars for the gene expression analysis. Our analysis revealed that OeTIR1 is coded by Oeu047472.1 and it contains 3 exons and codes for 594 amino acid protein and a coding sequence of 1785 nucleotides. Gene expression results showed different expression patterns between cultivars. Results indicate that in Gemlik cultivar expression of OeTIR1 does not show any upregulation after IBA application. Domat however showed an increase in expression only after 7 days.

Standardization of the Optimum Effects of Indole 3-Butyric Acid (IBA) to Control Root Knot Nematode, Meloidogyne enterolobii, in Guava (Psidium guajava L.).

Guava is an important revenue generating crop for small, medium, and commercial guava cultivators all over the world. Nematode infestation is one of the factors that cause declines in fruit production. Researches have proven that the application of plant growth regulators induces the synthesis of defense-related proteins in Guava. IBA is one such plant growth regulator, and its effects on guava plants has not yet been elucidated. Thus, this research is focused on the optimization of IBA concentrations, which results in the induction and production of maximum defense-related proteins to defend against root knot nematode. The present study includes the application of IBA on M. enterolobii-infested experimental guava plants at different concentrations ranging from 100 ppm to 2000 ppm. The synthesis of defense-related proteins is identified with 1000 ppm of IBA. At this concentration, IBA influences the morphological, physiological, and biochemical characteristics and enhances the induction of defense-related proteins in M. enterolobii-infested experimental guava plants. Thus, 1000 ppm of IBA prevents nematode infestation in Lucknow-49 guava plants.

EFFECTS OF DIFFERENT APPLICATIONS ON ROOTING CAPACITY OF BLACK ELDERBERRY CUTTINGS

Elderberry species (Sambucus spp.) grows mostly in wild form in the northern hemisphere. It is often used for landscaping riverbanks, as a windbreak, but also for human nutrition and medicinal purposes. This plant is used especially in the industrial field to obtain essence. In this study, the effects of some applications on rooting success in elder wood cuttings were investigated. For this purpose, in addition to 0, 2000, 4000 ppm IBA solution, a mixture solution of different Bacillus species (1.7X108 cfu/ml) was used. After the above-mentioned applications were applied to the wood cuttings taken from the plants during the resting period, they were taken into the perlite environment in the greenhouse at 22 0C. According to the results obtained, the number of roots per cutting was found to be between 11.3-22.4. Root lengths varied between 7.1-8.4 mm according to the applications. 2000 ppm IBA application was the application that increased both root length and root number the most. The study revealed that the rooting efficiency of black elderberry plant would be increased by IBA application.

Association of indolebutyric acid with rhizobacteria on the viability of herbaceous minicuttings of blueberry ‘Brite Blue’

Plant regulators used alone or associated with rhizobacteria are alternatives that maximize the rhizogenic potential of cuttings that are difficult to root, such as the blueberry. Thus, the objective of this study was to investigate if concentrations of IBA in talc, associated or not with the rhizobacteria Azospirillum brasilense and Bacillus subtilis, interfere in the viability of blueberry mini cuttings, cultivar ‘Brite Blue’. The experimental design used was entirely randomized, with four repetitions. The treatments studied, designed in a bifatorial scheme (4 × 3), were concentrations of IBA (0, 500, 1,000, and 2,000 mg L-1), applied as talc, associated or not with A. brasilense and B. subtilis. A significant interaction was observed between the factors IBA and inoculation with rhizobacteria regarding the presence of callus. In relation to the isolated effect of inoculation, B. subtilis proved to be superior to the treatments with application of IBA alone, also presenting the highest percentage of sprouted minestems. There was an isolated effect of IBA doses on the number of roots per minicutting, survival, and rooted minicutting. It is concluded that the treatments with IBA and rhizobacteria, especially B. subtilis, favor the viability of the ‘Brite Blue’ minicuttings.

Effect of Exogenous Application of Auxin on Leaf Cuttings of Mexican Snow Ball (Echeveria elegans Rose)

Aims: Succulents are on the rising trend of popularity due to its compromising behaviour with watering and durability indoors, creating a peaceful and healthy living condition. Thus it is the need of the hour to find a suitable propagation method or use of exogenous substances with propagation to assist regenerating new plants.
 Study Design: The experiment was laid out in randomized block design (RBD) with three replications.
 Place and Duration of Study: The present investigation entitled Effect of IAA and IBA Application on leaf cuttings of Mexican Snow Ball (Echeveria elegans). Was conducted in Research Field, Department of Horticulture, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, during February, 2021 to April, 2021.
 Methodology: The experiment was laid out in randomized block design (RBD) with three (IAA 100 ppm, IAA 300 ppm, IAA 500 ppm, IBA 100 ppm, IBA 300, IBA 500 ppm, IAA + IBA (100 ppm + 500 ppm), IAA + IBA (500 ppm + 100 ppm) and Control replications. The treatment in each replication was allotted randomly. Nine treatments having one variety were tried in the experimental design.
 Results: The results revealed that among all the treatments, application of IAA + IBA (100 ppm + 500 ppm) in treatment (T8) took minimum days to rooting (9.00), number of leaves (16.56), number of shoots (2.56), shoot height (15.56 mm), shoot diameter (16.44 mm), root length (29.00 mm) and in economic point of view treatment T8 IAA + IBA (100 ppm + 500 ppm) was found to be most economically viable in terms of gross return (Rs. 17,000), net return (Rs. 11,100) and benefit cost ratio (2.88).
 Conclusion: According to the present investigation it is concluded that treatment IAA + IBA (100 ppm + 500 ppm) was found most effective in terms of growth of Echevaria (Echevaria elegan).

Arbuscular mycorrhizal symbiosis enhances tomato lateral root formation by modulating CEP2 peptide expression.

Plant lateral root (LR) growth usually is stimulated by arbuscular mycorrhizal (AM) symbiosis. However, the molecular mechanism is still unclear. We used gene expression analysis, peptide treatment and virus-induced gene alteration assays to demonstrate that C-terminally encoded peptide (CEP2) expression in tomato was downregulated during AM symbiosis to mitigate its negative effect on LR formation through an auxin-related pathway. We showed that enhanced LR density and downregulated CEP2 expression were observed during mycorrhizal symbiosis. Synthetic CEP2 peptide treatment reduced LR density and impaired the expression of genes involved in indole-3-butyric acid (IBA, the precursor of IAA) to IAA conversion, auxin polar transport and the LR-related signaling pathway; however, application of IBA or synthetic auxin 1-naphthaleneacetic acid (NAA) to the roots may rescue both defective LR formation and reduced gene expression. CEP receptor 1 (CEPR1) might be the receptor of CEP2 because its knockdown plants did not respond to CEP2 treatment. Most importantly, the LR density of CEP2 overexpression or knockdown plants could not be further increased by AM inoculation, suggesting that CEP2 was critical for AM-induced LR formation. These results indicated that AM symbiosis may regulate root development by modulating CEP2, which affects the auxin-related pathway.

Impact of growth hormones on vegetative propagation of Elaeocarpus ganitrus Roxb.

Elaeocarpus ganitrus Roxb. commonly identified as ‘rudraksha’, belonging to Elaeocarpaceae possess a great religious, spiritual and materialistic significance which is placed under the threatened category in the tropical wet evergreen forests of North East India. Because of its over-exploitation, poor germination, low viability rate and hard seed coat, an attempt has been done for mass production of planting materials through cuttings and air layering’s. Cuttings trials were conducted during three different periods, mid - March, mid – July and mid – November by using Indol butyric acid and Napthalene acetic acid of 1000, 2000, 4000, 6000 ppm where air layering’s were done during mid - May by treating IBA and NAA solution of 500, 1000 and 2000 ppm in combination with para-hydroxybenzoic acid of 2000 ppm as synergist. Application of IBA and NAA of different concentration did not encourage the growth in the cuttings in all three periods. Air layering’s treated with NAA 1000 ppm+2000ppm para- hydroxy benzoic acid and IBA 500 ppm +2000ppm para- hydroxy benzoic acid resulted the highest and same value of callusing and rooting percent (70 and 66.67%), respectively while minimum callusing and rooting percentage (56.67 and 51.67%) was recorded in control. The present study focused on the mass production of planting materials in shorter duration with a reasonable cost for enhancing production as well as socio-economic conditions.

Image Analysis of Adventitious Root Quality in Wild Sage and Glossy Abelia Cuttings after Application of Different Indole-3-Butyric Acid Concentrations.

Adventitious root (AR) formation is a key step in stem cutting propagation of economically important woody ornamentals. Inadequate environmental and hormonal conditions can lead to the production of an insufficient or modest number of ARs in stem cutting, with a consequent decrease in quality. The aim of this research was to optimize wild sage and glossy abelia autumn stem cutting propagation protocols, using image analysis to assess the effects of different IBA concentrations and cultivars on AR quality. For both taxa, the treatments were: four IBA concentrations: 0, 1250, 2500 and 5000 mg L−1 and two cultivars: ‘Little Lucky’ (cv1) and ‘Yellow’ (cv2) from Lantana, and ‘Canyon Creek’ (cv1) and ‘Eduard Goucher’ (cv2) from Abelia. Results show that IBA application is not needed to enhance rooting ability; however, IBA concentration is an important factor determining the best overall AR quality in both taxa. In wild sage applying 5000 mg L−1 IBA improved AR quality in ‘Little Lucky’, increasing the root number, total length, surface area and number of forks and crossings, but decreased quality in ‘Yellow’. In glossy abelia ‘Edouard Goucher’, 5000 mg L−1 IBA increased the root number, but 1250 mg L−1 IBA improved AR quality; ‘Canyon Creek’ did not perform as well as cv2 at these concentrations. This study confirms that sensitivity to IBA dosage varies among species and their cultivars. Findings may help the commercial nursery industry produce higher quality cuttings.

PROPAGAÇÃO VEGETATIVA DE Mimosa Caesalpiniifolia PELA TÉCNICA DE MINIESTAQUIA

ABSTRACT This study aimed to establish a methodology for vegetative propagation via mini-cuttings technique for Mimosa Caesalpiniifolia. For such, three independent experiments were conducted: the first one evaluated survival and production of mini-stumps; the second tested the interaction between mini-cuttings types (apical and intermediate) and different concentrations of indolbutyric acid (IAB; 0, 2,000, 4,000 and 6,000 mg.L-1) on adventitious rooting; and the third analyzed the effect of leaf area reduction (0%, 25%, 50%, 75%, and 100%) on mini-cuttings. Mini-stumps survival at 180 days was 80%, with an average yield of 5 sprouts per mini-stump at 120 days. Apical mini-cuttings demonstrated a higher rooting percentage, without IBA application, higher than 80%. On the other hand, IBA application promotes increased rooting percentage in intermediate mini-cuttings. Treatments without leaf reduction and with reduction of 25% promoted better results concerning rooting and plant development. Results support the hypothesis that mini-cuttings technique is viable for the propagation of Mimosa Caesalpiniifolia.

EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO AND EX VITRO CONDITIONS ON PROPAGATION OF DIANTHUS CARYOPHLLUS L.

This research was carried out in the tissue culture Laboratory at University of Baghdad during 2017-2018 in order to evaluate the effect of plant growth regulators on the propagation of two cultivars of Dianthus (Sun charm White and Master) under Ex vitro conditions by cultivating the single nodes using the MS medium which involved with different concentrations of growth regulators. The interaction treatment 1.0 mg /L BA + 0.2 mg /L NAA gave the highest response percent of bloom reached 100% for the red cultivar, and the treatment 1.5 mg / L BA + 0.2 mg / L gave the highest response percent to the white cultivar bloom reached 90%.In the multiplication stage, the highest branches number was obtained at 4.5 branches by the application of 2.0 mg / L BA + 0.2 and 0.4 mg /L IAA for the red cultivar, while the white variety gave the highest branches number was obtained at the treatment 2.0 mg /L BA + 0.1 mg /L IAA, which gave 4.8 branches. During the rooting stage, the application of IBA at a concentration of 1 mg / L for the red and white cultivars obtained the highest rooting ratio and the highest root length reached 70% and 2.01 cm respectively for red, while the highest rooting ratio and root length of 80% and 2.19 cm respectively were given for white cultivar, and the highest root number at a concentration of 1.5 mg /L, reached 2.50 for red and 2.30 for white. In the experiment of rooting under Ex Vitro conditions, the results revealed that the concentration of 2 mg / L IBA gave the highest rooting ratio, number, and length of roots reached 50%, 1.40 and 1.23 cm respectively for the red cultivar, while the white cultivar was gave, 70%, 1.60 and 1.20 cm respectively, and the plants were adapted under greenhouse conditions.

Effect of growth regulators (IBA and NAA) on rooting and survival of guava air layers

The present investigation was conducted at Fruit Nursery, Department of Horticulture, College of Agriculture, Gwalior during the year 2015-16 on guava cv. Gwalior-27. The treatment combinations comprised with four concentration of IBA viz., 0ppm (I0), 7500ppm (I1), 10000ppm (I2) and 15000ppm (I3) and four concentration of NAA viz., 0ppm (B0), 50ppm (N1), 100ppm (N2) and 150ppm (N3). Results revealed that higher concentration of IBA (15000 ppm) and NAA (150 ppm) gave significantly highest results on callusing, number of primary and secondary roots, root weight per air layer, rooting and survival percentage over other lower concentrations while, combined application of higher concentration of both the growth regulators (I3N3) also gave higher results for all the parameters however, it was statistically at par with application of IBA @ 15000 ppm + NAA @ 100 ppm (I3N2) for all the parameters except survival percentage.

Impact of Auxins on Rooting and Establishment of Seedless Lemon (Citrus limonL. Burm.) Air-layers for Successful Propagation

Study Design: Randomized Block Design.
 Place and Duration of Study: Department of Horticulture, College of Agriculture, RVSKVV, Gwalior, MP between 2018 - 2019.
 Methodology:In 2018-19 the study conducted amidst rainy season and Gird agro-climatic context of Madhya Pradesh to evaluate the effect of phytohormones on rooting and establishment of seedless lemon air layers. Experiment consisting nine treatments replicated three times under Randomized Block Design. Treatments comprising of two plant hormones namely IBA and NAA each with four concentration levels i.e. 1250, 2500, 3750 and 5000ppm including control. Plants age were seven years.
 Results:The results revealed maximal rooting percentage (92.00), number of primary roots (22.72) and secondary roots (49.97), length of primary roots (5.05 cm) and secondary roots (2.25 cm), diameter of primary roots (1.68 mm) and secondary roots (0.84 mm) and dry weight of roots (2.32g) and planted air-layers (89.21 %) were recorded with application of IBA @ 5000ppm.
 Conclusion:Indole-3-butyric acid performed better than naphthalene acetic acid for promoting rooting and establishment in seedless lemon air-layers. Application of IBA @ 5000ppm significantly increased rooting characters followed by NAA @5000ppm for better establishment of Lemon air layers for its propagation.

Taxonomy and Grafting of Ornamental Cacti: A Review

Nowadays, cacti and succulents are widely utilized in landscaping. These plants suit very effectively for both indoor and outdoor decorations. The family Cactaceae comprises of 130 genera with 2000 species and this is primarily divided into three tribes namely: Pereskieae, Opuntieae and Cereeae. Cactus flowers are very attractive and are diverse in size, number, form and color. The nocturnal flowers are always white, with light-yellow or red tones, whereas diurnal flowers are white, purple, yellow-orange, red or green. Basically, an areole is a transformed axillary bud situated over a tubercle with spines and often with hairs and trichomes. Due to their low maintenance requirements and their ability to withstand harsh environments, cacti are extremely popular as ornamental plants across the planet. Cacti can be propagated by seeds, cuttings, offsets and grafts. Grafted cacti are now regarded as one of the most admired ornamental indoor plants worldwide. In cacti, grafting has become a commercial method of propagation to accelerate and hasten the growth rate of slow growing species, to ensure the survival of the plants with poor root system, to ensure the survival of genetic aberration of variegated and bright coloured cacti (red and yellow caps) that lack chlorophyll, to accelerate the growth of plants for commercial use, in order to keep cristate and monstrose forms look attractive. Successful grafts have been obtained when the species like Trichocereus spachianus, Trichocereus macrogonus, Trichocereus pachanoi, Hylocereus trigonus, Hylocereus undatus, Selenicereus grandiflorus, Opuntia bergeriana, Opuntia ficus-indica are grafted onto Nopalea spp. The exogenous application of IBA has a positive effect on the vegetative growth of grafted plants using (Gymnocalycium mihanovichii scion grafted onto Trichocereus spachianus rootstock.