Promoting Rooting and Vegetative Growth in 41 B Vine Rootstock Cuttings with Encapsulated Nano Silver and IBA Applications

Abstract

Globally, viticulture is carried on by grafting onto vine rootstocks obtained by hybridizing different vitis species or on their own roots due to their tolerance to biotic and abiotic stresses, especially phylloxera (Daktulosphaira vitifoliae). Sustainable regeneration of vineyard areas and new vineyard establishments require significant amounts of vine saplings each year. In addition to hormonal applications, nano products are also tested in the production of high quality vine nursery material. In this study, single nodal cuttings prepared from the vine rootstock 41 B (Vitis vinifera L. cv. Chasselas × Vitis berlandieri) in the resting period were used as plant material. The effects of encapsulated nano silver particles (AgNPs, 0.5 mg L-1 and 1 mg L-1), and IBA (0 and 1 g L-1) applications produced by green synthesis with cv. Öküz Gözü grape seed extract and AgNO3 on promoting rooting and vegetative growth in 41 B vine rootstock cuttings were evaluated in this study. AgNPs and IBA applications promoted rooting of cuttings, and vegetative growth of rooted cuttings. Applications increased shoot length, shoot diameter, number of nodes, fresh and dry weights of roots and shoots, and the highest value among these was achieved by the 1 mg L-1 AgNPs application. Leaf area and chlorophyll content (SPAD value) were determined by 1 g L-1 IBA application. AgNPs and IBA treatments resulted in increased rooting and vegetative growth compared to control. In conclusion, 1 mg L-1 AgNPs was recommended for vine propagation material and sapling producers as nano promotors for rooting and vegetative growth of 41 B rootstock cuttings.