EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO AND EX VITRO CONDITIONS ON PROPAGATION OF DIANTHUS CARYOPHLLUS L.

Abstract

This research was carried out in the tissue culture Laboratory at University of Baghdad during 2017-2018 in order to evaluate the effect of plant growth regulators on the propagation of two cultivars of Dianthus (Sun charm White and Master) under Ex vitro conditions by cultivating the single nodes using the MS medium which involved with different concentrations of growth regulators. The interaction treatment 1.0 mg /L BA + 0.2 mg /L NAA gave the highest response percent of bloom reached 100% for the red cultivar, and the treatment 1.5 mg / L BA + 0.2 mg / L gave the highest response percent to the white cultivar bloom reached 90%.In the multiplication stage, the highest branches number was obtained at 4.5 branches by the application of 2.0 mg / L BA + 0.2 and 0.4 mg /L IAA for the red cultivar, while the white variety gave the highest branches number was obtained at the treatment 2.0 mg /L BA + 0.1 mg /L IAA, which gave 4.8 branches. During the rooting stage, the application of IBA at a concentration of 1 mg / L for the red and white cultivars obtained the highest rooting ratio and the highest root length reached 70% and 2.01 cm respectively for red, while the highest rooting ratio and root length of 80% and 2.19 cm respectively were given for white cultivar, and the highest root number at a concentration of 1.5 mg /L, reached 2.50 for red and 2.30 for white. In the experiment of rooting under Ex Vitro conditions, the results revealed that the concentration of 2 mg / L IBA gave the highest rooting ratio, number, and length of roots reached 50%, 1.40 and 1.23 cm respectively for the red cultivar, while the white cultivar was gave, 70%, 1.60 and 1.20 cm respectively, and the plants were adapted under greenhouse conditions.