Abstract

Adventitious root (AR) formation is a key step in stem cutting propagation of economically important woody ornamentals. Inadequate environmental and hormonal conditions can lead to the production of an insufficient or modest number of ARs in stem cutting, with a consequent decrease in quality. The aim of this research was to optimize wild sage and glossy abelia autumn stem cutting propagation protocols, using image analysis to assess the effects of different IBA concentrations and cultivars on AR quality. For both taxa, the treatments were: four IBA concentrations: 0, 1250, 2500 and 5000 mg L−1 and two cultivars: ‘Little Lucky’ (cv1) and ‘Yellow’ (cv2) from Lantana, and ‘Canyon Creek’ (cv1) and ‘Eduard Goucher’ (cv2) from Abelia. Results show that IBA application is not needed to enhance rooting ability; however, IBA concentration is an important factor determining the best overall AR quality in both taxa. In wild sage applying 5000 mg L−1 IBA improved AR quality in ‘Little Lucky’, increasing the root number, total length, surface area and number of forks and crossings, but decreased quality in ‘Yellow’. In glossy abelia ‘Edouard Goucher’, 5000 mg L−1 IBA increased the root number, but 1250 mg L−1 IBA improved AR quality; ‘Canyon Creek’ did not perform as well as cv2 at these concentrations. This study confirms that sensitivity to IBA dosage varies among species and their cultivars. Findings may help the commercial nursery industry produce higher quality cuttings.

Highlights

  • Woody species can be propagated using two different methods: vegetative multiplication, which produces clones, and sexual reproduction [1,2]

  • The aim of this study was to optimize wild sage and glossy abelia autumn stem cutting propagation protocols, using image analysis to assess the effects of different indole-3-butyric acid (IBA)

  • The results of this study clearly show that vegetative propagation of wild sage and glossy abelia via semi-hardwood stem cuttings taken in early autumn is possible

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Summary

Introduction

Woody species can be propagated using two different methods: vegetative multiplication, which produces clones, and sexual reproduction [1,2]. Vegetative propagation is the most popular method, as it is cheaper, easier and faster than sexual reproduction [5]; the plants produced are clones that maintain the morpho-physiological and genetic characteristics of the stock plants, uniformity and earliness of production [6]. A number of techniques may be adopted in the vegetative propagation of woody plants. In-vitro propagation allows the production of a large number of plants in a very short time [11,12]; this technique requires equipped laboratories and skilled labor [13]. Propagation by stem cutting, less expensive and easier than in-vitro

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