Abstract
Micropropagation of Chrysanthemum is an alternative to the traditional method of reproduction. Thanks to this method, the Chrysanthemum reproduction time is reduced to 3-4 months. For clonal micropropagation, sterile microshoots of Chrysanthemums of the varieties Snow White, Stranger, and Baltica White were used. At the stage of the micropropagation, the Murashige-Skoog (MS) medium with the full and half composition of mineral salts and growth regulators (kinetin, 6-benzylaminopurine, β-indolylacetic acid) were used. A universal culture medium for clonal micropropagation of all varieties of Chrysanthemum and optimal mediums, taking into account variety-specificity were established. It was noted that under in vitro conditions, high proliferative activity was observed in Neznakomka variety.
Highlights
The floriculture industry has become one of the most important branches of agriculture
Biotechnological methods are widely used in the propagation of Chrysanthemums in an in vitro culture, as evidenced by numerous publications (Datta, 2012; Deein et al, 2013a, b; Kolomiets, Malyarovskaya, 2013; Kulus et al, 2013; Kuritskaya et al, 2016; Lim et al, 2012; Shinoyama et al, 2006; Teixeiria da Silva et al, 2013; Tymoszuk A., Zalewska M., 2014; Zhou et al, 2014)
In our studies, the variety-specificity of Chrysanthemums during clonal micropropagation was confirmed, which is noted in the works of Granda Hamillo (2009)
Summary
The floriculture industry has become one of the most important branches of agriculture. Decorative Chrysanthemums are one of the first flower crops on the world market (Teixeira da Silva, Kulus, 2014). They have been cultivated for over 2000 years. Biotechnological methods are widely used in the propagation of Chrysanthemums in an in vitro culture, as evidenced by numerous publications (Datta, 2012; Deein et al, 2013a, b; Kolomiets, Malyarovskaya, 2013; Kulus et al, 2013; Kuritskaya et al, 2016; Lim et al, 2012; Shinoyama et al, 2006; Teixeiria da Silva et al, 2013; Tymoszuk A., Zalewska M., 2014; Zhou et al, 2014). Reducing the time of growing Chrysanthemums to a product standard is an urgent problem. The purpose of our research is to increase the efficiency of clonal micropropagation of Chrysanthemum
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