Optimization of Nutrient Media of in Vitro Propagation for Some Cherry Rootstocks (Prunus Cerasus)

Abstract

The most important species of which have origin many populations, land-races and local varieties: wild cherry, wild pears, wild almond, wild medlar, etc. This study was focused in many important rootstocks of P. avium x P canascens - Gisela 6, P.avium x P. pseudocerasus - Colt (nano), P. mahaleb x P.avium (Maxma), P. Mahaleb and cultivar Prunus avium L. (Cerasus avium L. var. sativa Ser., P. cerasus): Bigarreau Hatif. Are used two important media in the micro propagation of the horticultural species above, MS (Murashige & Skoog) and LP (Quoirin & Lepoivre). Nutrients of MS media have affected with sensible changes compare with LP media, in the explants proliferation for differentiations of the buds, shoots and leafs. Inoculation of explants in LP media, results with lower result on the qualitative indicators of the research. Average growing of the shoots on the MS media, resulted 10.1 mm while on the LP media was 6.0 mm. Average number of leafs 2.47 on the MS media, while on the media LP was 1.34 leaf. In the statistic analyses for the min and max, in all the cases it has predominance of the MS media. Intensity of the growing has cv (coefficient of variation) = 63% and on the MS media results 1.88 highest of LP media. Keywords: Murashige & Skoog, Quoirin & Lepoivre, buds, leafs, shoots.