Induksi Kalus Binahong (Basella rubra L.) Secara In Vitro Pada Media Murashige & Skoog Dengan Konsentrasi Sukrosa Yang Berbeda

Abstract

Binahong (Basella rubra L.) is a plant medicine consisting secondary metabolites which have virtue as medicines for several diseases that could also be used as coloring agent. The medicine compounds in secondary metabolites could be extracted from callus. Sucrose is one of the components that build MS (Murashige & Skoog) medium. Sucrose is important in in vitro culture, it functions as carbon and energy source for explant to grow. The purposes of this research are to study the effect of sucrose in MS medium towards B. rubra L callus formation and growth; to find the optimum sucrose concentration for callus B. rubra L formation; and also to find the fastest initiation time to produce callus crumb. This research uses Complete Random Design (CRD) single factor method, i.e., sucrose concentration of 0 g/l, 10g/l, 20 g/l, 30 g/l, 40 g/l with five repetitions. The data is analysed with Analysis of Varian (Anova) and if a real difference is found the analysis is continued with Duncan Multiple Range Test (DMRT) with significancy level of 95%. The results show that various sucrose concentrations in MS medium influences callus B. rubra L induction. The highest sucrose concentration, i.e. 40 g/l, which was added into MS medium, could induce the maximum callus wet-weight of 1,69 g and the fastest callus initiation time of 4,8 day.