Abstract
ABSTRACTWe report the draft genome sequences of a novel member of the Picornavirales isolated from Pacific abalone (Haliotis discus hannai). The full length of the assembled draft genome sequences, obtained by use of a next-generation sequencing technique, were 8,019 nucleotides, including an RNA-dependent RNA polymerase gene (5,088 nucleotides) and a capsid protein gene (2,553 nucleotides). This genome sequence will be useful for understanding viral disease of Pacific abalone.
Highlights
We report the draft genome sequences of a novel member of the Picornavirales isolated from Pacific abalone (Haliotis discus hannai)
Soft tissue isolated from the Pacific abalone Haliotis discus hannai was homogenized in 1 mL of RiboEx (GeneAll, Daejeon, South Korea) in a glass homogenizer vessel, and RNA was isolated with chloroform and isopropanol [8]
RNA was purified using a Hybrid-R column (GeneAll) according to the protocol provided by the manufacturer
Summary
We report the draft genome sequences of a novel member of the Picornavirales isolated from Pacific abalone (Haliotis discus hannai). Soft tissue isolated from the Pacific abalone Haliotis discus hannai was homogenized in 1 mL of RiboEx (GeneAll, Daejeon, South Korea) in a glass homogenizer vessel, and RNA was isolated with chloroform and isopropanol [8]. RNA was purified using a Hybrid-R column (GeneAll) according to the protocol provided by the manufacturer.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
