Abstract
Streptomyces sp. SMS_SU21 possesses strong antimicrobial activity and antioxidant potential. This strain was isolated from the Sundarbans mangrove ecosystem, and its draft genome comprises 7,449,420 bp with 6,680 open reading frames. Genome analysis of strain SMS_SU21 provides insight into its secondary metabolite arsenal and reveals the gene clusters putatively responsible for its bioactive potential.
Highlights
Genomic DNA was extracted with a HiPurA streptomycetes DNA isolation and purification kit (Himedia, India)
Shotgun sequencing was performed with a highthroughput HiSeq platform (Illumina) at AgriGenome Labs Private Limited in Kerala, India
The cleaned reads were subjected to analysis with Kmergenie (6) to predict the optimal k-value and assembly size, which were found to be 31 and 7,449,420 bp, respectively
Summary
Genomic DNA was extracted with a HiPurA streptomycetes DNA isolation and purification kit (Himedia, India). Shotgun sequencing was performed with a highthroughput HiSeq platform (Illumina) at AgriGenome Labs Private Limited in Kerala, India. Prior to whole-genome analysis, Cutadapt version 1.8 (4) was used to remove adapter sequences, and all low-quality data (Q Ͻ 30) were filtered out using Sickle version 1.33 (5). The cleaned reads were subjected to analysis with Kmergenie (6) to predict the optimal k-value and assembly size, which were found to be 31 and 7,449,420 bp, respectively.
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