Abstract
The thermophilic bacterium Geobacillus thermoleovorans was isolated from a tropical air sample collected in Singapore. The genome was sequenced on the PacBio RS II platform and consists of one chromosome with 3.6 Mb and one plasmid with 75 kb. The genome comprises 3,509 protein-coding genes, 88 tRNAs, and 27 rRNAs.
Highlights
The thermophilic bacterium Geobacillus thermoleovorans was isolated from a tropical air sample collected in Singapore
The reads were subsequently used for de novo assembly with Hierarchical Genome Assembly Process (HGAP) version 3 [8], which is included in the PacBio single-molecule real-time (SMRT) Analysis 2.3.0 package
Functional annotation with Rapid Annotations using Subsystems Technology (RAST) [13,14,15] showed that 116 genes were associated with dormancy and sporulation, which could indicate the ability to enter long-term survival states in hostile environments
Summary
The thermophilic bacterium Geobacillus thermoleovorans was isolated from a tropical air sample collected in Singapore. Sequencing resulted in a total of 55,892 subreads. The reads were subsequently used for de novo assembly with Hierarchical Genome Assembly Process (HGAP) version 3 [8], which is included in the PacBio SMRT Analysis 2.3.0 package.
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