Abstract
Summer mortality, caused by thermal conditions, is the biggest threat to abalone aquaculture production industries. Various measures have been taken to mitigate this issue by adjusting the environment; however, the cellular processes of Pacific abalone (Haliotis discus hannai) have been overlooked due to the paucity of genetic information. The draft genome of H. discus hannai has recently been reported, prompting exploration of the genes responsible for thermal regulation in Pacific abalone. In this study, 413 proteins were systematically annotated as members of the heat shock protein (HSP) super families, and among them 26 HSP genes from four Pacific abalone tissues (hemocytes, gill, mantle, and muscle) were differentially expressed under cold and heat stress conditions. The co-expression network revealed that HSP expression patterns were tissue-specific and similar to those of other shellfish inhabiting intertidal zones. Finally, representative HSPs were selected at random and their expression patterns were identified by RNA sequencing and validated by qRT-PCR to assess expression significance. The HSPs expressed in hemocytes were highly similar in both analyses, suggesting that hemocytes could be more reliable samples for validating thermal condition markers compared to other tissues.
Highlights
Abalone is a shellfish belonging to the family Haliotidae
96.7 Gb remained after filtering out sequencing artifacts and 76.5 Gb were mapped to the H. discus hannai draft genome (Supplementary Figure S1)
Hemocytes exhibited the highest differential expression compared to other tissues, including four specific heat shock protein (HSP)
Summary
Abalone is a shellfish belonging to the family Haliotidae (class: Gastropoda). Its growth and development are influenced by various environmental factors, such as temperature, oxygen, CO2 , and salinity. Temperature is the most important factor, having a higher correlative effect with other stressors as well as increased summer mortality rates in abalones and other ectothermic slow-crawling shellfishes [1]. Increases in atmospheric temperature are reflected in the decreased oxygen solubility in coastal waters, which creates hypoxic conditions for various aerobic underwater organisms, causing them to experience internal energy imbalances [2]. These stressed shellfishes are highly susceptible to pathogens, increasing the mortality rate in aquaculture systems [3]. In Pacific abalone (H. discus hannai), deviations from the optimal temperature (20 ◦ C) resulted in suppressed lysozyme activity, reducing immune activity against bacterial infection and leading
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
