Percentage Yield Research Articles

The Impact of Marine Resource-Free Diets on Quality Attributes of Atlantic Salmon

The influence of feeding Atlantic salmon for 90 days on diets that excluded fishmeal (FM) and fish oil (FO) was examined for influence on various quality traits. In addition, the effect of adding krill meal (KM; 0%, 2.5%, and 5%), as a putative feed palatant was also examined. Total replacement of FM/FO had a limited effect on production characteristics, affecting percentage yields of headed and gutted control fish and their standard length (p < 0.05). Variances between dietary groups were observed for pigmentation, and plant protein-based KM-free-fed fish returned deeper hues across their belly, NQC (Norwegian Quality Cut), and back portions (p < 0.03). No differences were measured for relative fin condition. δ13C and δ15N concentrations were lower and higher, respectively (p < 0.05) for fish fed the FM/FO-based diet. δ13C:δ15N likewise differed between treatments with FM/FO-fed salmon expressing higher ratios. Fillet mechanical characteristics varied with fish fed on animal protein-based diets, without KM expressing higher springiness and resilience (p < 0.05). Fish fed plant-based diets were generally preferred by younger taste testers. The results from this trial illustrate that FM/FO can be completely removed from salmon diets without problematic effects on quality and palatability attributes.

Quantitative estimation of metabolites and antifungal efficacy of leaf extracts <i>Aspilia africana</i> on cucumber and pawpaw fruit spoilage fungi

Several reports have shown that about 25% of harvested fruits globally are lost to spoilage by microorganisms. The study determined the quantitative metabolites and bioactivities of Aspilia africana extracts on fungi from spoiled Cucumis sativus and Carica papaya fruits. The fungi from spoiled C. sativus and C. papaya fruits were obtained using mycological techniques. The quantitative metabolites and bioactivities of the aqueous (ALEAA) and ethanol (ELEAA) extracts of A. africana were determined using the standard protocol and disc diffusion technique, respectively. The fungal genera obtained were Aspergillus, Penicillium, Mucor, Fusarium, and Rhizopus. There were variations in percentage yields, physical appearances, and pH of the extracts. The results of quantitative estimation of metabolites showed that ALEAA had mean protein, carbohydrate, and lipid contents of 15.36 ± 0.32%, 60.97 ± 1.14%, and 6.66 ± 0.04%, respectively. Alkaloids showed a positive correlation with protein (r = 0.2028) and carbohydrate (r = 0.421), and a negative correlation with lipid (r = -0.6556) at p < 0.05. The ELEAA exhibited more inhibitory effects on test fungal isolates, with mean zones of inhibition (IZs) ranging from 9.3 ± 0.1 to 18.8 ± 0.3 mm, than the ALEAA, with mean IZs ranging between 9.4 ± 0.4 and 16.0 ± 1.0 mm. The R coefficients of the extracts and IZs as exhibited by the fungi ranged from 0.5985 to 0.9936. The results have revealed quantitative metabolites and antifungal activities of the extract and also provided rationale for its utilization as a preservative for fruits against spoilage by fungi.

DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENT AND EVALUATION OF ANTIOXIDANT ACTIVITIES OF CUSCUTA REFLEXA

Background: The aim of the study was to identify functional groups and antioxidant activity of the Cuscuta reflexa (C. reflexa) stems extract. Due to the reach of medicinal components, it has been using among Indian subcontinent people to treat various chronic diseases for ancient era. However, there are lacks of study on antioxidant activities of C. reflexa with its potential therapeutic compounds, therefore this study motivated to investigate more on its stems extract for further explore. Methods: Soxhlet extraction method was employed to extract phenolic compounds from C. reflexa stems. The effect of extraction time (1-4 h) and concentration of ethanol (45%, 60%, 75% and 90%) on the percentage of yield, total phenolic (TPC) and flavonoid content (TFC) was investigated. The functional groups of phenolic compounds were characterized by using Fourier Transform Infrared Spectrometry (FTIR). DPPH and ABTS•+ radical scavengers were used to evaluate antioxidant activity. Results: Data showed the highest percentage of yield (10.22±0.14% w/w), TPC (64.11±0.17%, mg GAE/g d.w.) and TFC (41.08±0.34%, mg QE/g d.w.) at 3 h with 75% ethanol. FTIR results revealed the presence of functional groups associated phenolic compounds which are the band at 1260 cm−1 illustrations the C–O groups of polyols existence and C – O –C of ester for presence of quercetin which represent the hydroxy flavonoids in the extract. DPPH and ABTS•+ radical scavengers were showed very potent antioxidant activity with IC50 295.12±1.33 and 245.43±0.78 μg/mL. Conclusion: Phenolic and flavonoids enriched C. reflexa extract may play a potential role as a natural nutritional and therapeutic source in Bangladesh. Peer Review History: Received: 27 October 2023; Revised: 19 November; Accepted: 28 December, Available online: 15 January 2024 Academic Editor: Dr. Ali Abdullah Al-yahawi, Al-Razi university, Department of Pharmacy, Yemen, alyahawipharm@yahoo.com Received file: Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.my Prof. Ali Gamal Ahmed Al-kaf, Sana'a university, Yemen, alialkaf21@gmail.com

Preparation and Evaluation of Ketoprofen Loaded Mucoadhesive Microspheres

The aim of the present study was to prepare and evaluate Ketoprofen loaded microspheres fabricated with sodium alginate and Carbopol 934 by ion gelation method which enhances the drug residence time into the stomach by adhering to the mucus layer of and control the rate of drug release for longer period of time. Ketoprofen is a nonsteroidal anti-inflammatory drug (NSAID) that is commonly used for its analgesic, anti-inflammatory, and antipyretic (fever-reducing) properties. It belongs to the propionic acid class of NSAIDs and is available in various formulations, including oral tablets, capsules, topical gels, creams, and as an injectable solution. In present study ketoprofen incorporates in mucoadhesive drug delivery system for utilizing its controlled drug delivery up to 12 hrs. In present study prepared microspheres were evaluated for- particle size, entrapment efficiency, percentage yield, morphology, mucoadhesive property, in-vitro drug release property.
 Keywords: Mucoadhesive microspheres, NSAID, ion gelation method, ketoprofen

Impact of Front Line Demonstrations on the pulses and oilseed productivity in South Tripura district

Krishi Vigyan Kendra, South Tripura undertaken number of front line demonstration (FLD) to impart knowledge on different improved scientific technologies of non-traditional oilseeds and pulses crops like groundnut, lentil, red gram (arhar) and green gram (moong) during Kharif and Rabi season of 2009-10. To demonstrate the technology, six villages of South Tripura district of Tripura state were selected after assessment of the village through PRA method. Awareness programme were conducted in all villages. Farmers were trained on scientific cultivation practices of groundnut, lentil, red gram (Arhar) and green gram (Moong). Besides imparting training, printed leaflets on oilseed and pulses were distributed among the farmers. Field day programme was also conducted in the farmer’s field during field demonstration. Altogether 8 hectare area of oilseed and 5 ha area of pulses was covered under this programme. Improved varieties of groundnut (GG-20, GG-7 and TG-37A), lentil (WBL-58), green gram (HUM-16) and red gram (UPAS-120) were distributed among the farmers. Under this programme, most of the farmers obtained better yield of pulses and oilseeds as compared to local varieties and traditional method of cultivation. The yield percentage increased from 21.43 to 40 per cent in case of pulses, and 30.43 to 65.45 per cent in oilseed crops. Farmers earned up to Rs.31,100/ha by selling oilseed and up to Rs. 29,250/ha by selling pulses crops. The programme conducted by KVK, South Tripura succeeded to build the skills of farmers for expansion of pulses and oilseeds crops through number of knowledge management practices like group meetings, awareness programmes, trainings, demonstrations, field day, etc. Farmers of the neighbouring villages also participated in this programme and expressed their willingness to take up such crops under scientific methods in the next crop season.

Comparison between Developing and Nondeveloping Disturbances for Tropical Cyclogenesis in Different Large-Scale Flow Patterns over the Western North Pacific

Abstract This study classifies 407 developing disturbances (DEV) and 2309 nondeveloping disturbances (NONDEV) over the western North Pacific into five large-scale circulation patterns, namely the pre-existing cyclone (PC), easterly wave (EW), zonal wind convergence (CON), zonal wind shear line (SL), and mixed zonal wind convergence and shear line (CON-SL) patterns. The SL pattern has the highest TC yield percentage, followed by the CON-SL, while the EW is the least favorable pattern. The composite analysis shows that upper-level divergence, midlevel relative humidity, and surface heat flux (SHF) growth are crucial to the disturbance development in all the five patterns. Besides, large lower-level barotropic kinetic energy conversion and a well-developed primary circulation are good indicators for disturbance development in the PC, EW, and CON rather than in the SL and CON-SL patterns. Furthermore, for the PC, EW and CON patterns, the DEV features strong and rapidly growing SHF and mesoscale convective systems (MCS) closer to the disturbance center, which allows deep-layer warming and moistening, and drives a deep secondary circulation. Interestingly, due to an environment with high lower-level vorticity, the SL and CON-SL patterns typically foster a relatively mature primary circulation with strong SHF and MCS concentrated close to the center, especially for the NONDEV at the pre-genesis stage. However, a drier mid-to-upper-level environment for the NONDEV inhibits deep convection, which may explain its shallow secondary circulation and therefore poor potential to develop further.

Impact of frontline demonstration on groundnut productivity in South Tripura district

In South Tripura district groundnut is grown in very small pocket using local varieties. In general, farmers do not follow scientific package of practices and not using the improved variety due to which the productivity of groundnut is only 1200 kg/ha. With a view to increase the productivity level of groundnut in the district total 80 numbers of demonstration has been taken covering 17 ha area in six selected villages during Kharif and Rabi season of 2011and 2012 by KVK, South Tripura. The objective of the FLD on groundnut was to demonstrate the potential of improved varieties and technologies to the farmers. Awareness programme and training on scientific cultivation practices of groundnut was conducted before starting the demonstration programme. Besides imparting training, printed leaflets on groundnut were distributed among the farmers for their ready reference. Field day programme was also conducted in the farmer’s field in standing crop. Improved variety of groundnut viz. GG-7 and GG- 20 were demonstrated for obtaining higher yield as compared to local varieties and traditional method of cultivation. Increase in yield percentage varies from 26.83 to 50.43% for both the varieties over the local check. Farmers earned upto Rs. 38550/- for both the varieties by selling the groundnut in the local market.

Green synthesis of 1,3-Thiazolidin-4-ones derivatives by using acid-activated montmorillonite as catalyst

An environmentally friendly, clean, and applicable methodology for synthesizing 1,3-Thiazolidin-4-one derivatives was developed. This method produced the target compounds in high yields and short reaction times by using solvent-free reaction conditions with heterogeneous catalysts, montmorillonite (MT) and acid-activated montmorillonite (acid-MT). MT and acid-MT were investigated in the synthesis using a variety of techniques, including conventional heat, ultrasonic, and microwave irradiation, with the investigation of two types of factors, namely time and temperature. X-ray diffraction, N2 adsorption–desorption, Fourier transform infrared spectroscopy, scanning electron microscopy, and transmission electron microscopy were used to characterize the MT and acid-MT catalysts. FTIR, 1H NMR, 13C NMR, and mass spectra were used to characterize each product. The ring-cyclization step, with its short time and high yield percentage, is the most important factor for the strength of the prepared catalyst, acid-MT. Additionally, the acid-MT catalyst was easily recoverable and can be reused up to five times without suffering a substantial reduction in catalytic activity.

Yield gap variation in rice cultivation in Indonesia

Abstract The rice yield gap (YG) is a global concern, requiring more detailed studies spatially and temporally. As a staple food in Indonesia, rice was produced from 7.4 Mha paddy fields in 2019. Better insight into the YG helps assess measures to boost rice production. However, the information on YG variation among regions scale is limited. This study aimed to identify the rice YG based on 295 historical trial datasets from 23 provinces in Indonesia. We surveyed published trial results from 2012 to 2022 and analyzed YGs, expressed as the percentage of farmer yield (FY). The potential yield (PY) was estimated from field trial results using introduced rice cultivation technology package, whereas FY from results using existing farmer practices. Our study showed that the average YG was 62% in rainfed, 54% in tidal, and 32% in irrigated paddy fields. The YG was significantly high in the paddy fields of Kalimantan (74%) and Maluku-Papua (49%), while the lowest was in Sulawesi (27%) and Java (31%). The YG varied significantly with geo-regions, rice varieties, and cultivation technology packages. Closing the YG and ensuring sustainable rice production requires the implementation of sustainable intensification through applying site-specific technology packages, reallocation of agricultural interventions to a higher YG region, and rice variety improvement to increase PY.

Wheatgrass (Triticum Aestivum) Extract Loaded Chitosan Solid Lipid Nanoparticles: Formulation, Physicochemical Characterisation and Cytotoxic Potential.

The prevalence of cancer is around the world, and is identified as a multifarious ailment. Amongst the most common reasons for cancer in the world is oxidative stress, and this can be overcome by taking the herbal plant wheatgrass in any form. The aim of the present work is to formulate wheatgrass extract-loaded solid lipid nanoparticles using Box-Behnken design and to investigate the effect of formulation variables. Using the hot homogenisation method, the current work plan aimed to develop wheatgrassfilled chitosan solid lipid nanoparticles by means of a Box-Behnken design. This study investigated the effect of three formulation variables on particle size and entrapment efficiency, namely the sodium alginate concentration, the chitosan concentration, and the sonication time. Extraction of wheatgrass was done in a soxhlet extractor, using methanolic extract. Furthermore, the authors have examined recent patents associated with wheatgrass to enhance their comprehension of this herbal plant. The hot homogenisation technique was used to prepare Triticum aestivum extract loaded with solid lipid nanoparticles (SLNs). For BBD, all formulations were analysed for particle size, which ranged from 394.4 to 911.2 nm, and for polydispersity index, which ranged from 0.527 to 1.0. Batch code BB SLN-8 was found to be the finest suitable because of a maximum loading capacity of 58.23 ±0.11 % (w/w), maximum entrapment efficiency of 55.12±0.17 % (w/w), and minimum particle size of 394.4nm by using sodium alginate as a surface stabiliser at sonication time ~ 10 min and having maximum percentage yield of 49.87%. During characterisation studies and MCF-6 cell line studies, it was found that wheatgrass has antioxidant potential and is potent against breast cancer. As an alternative medicine for cancer, wheatgrass is considered to be effective due to its high antioxidant content.

Flammability and thermal resistance of Ceiba petandra fiber‐reinforced composite with snail powder filler

AbstractNatural fiber composites are not suitable for use in industries due to their flammability and low thermal resistance. Inorganic metal‐based fillers are known to enhance the flammability and thermal resistance of composites, while the thermal and fire‐retardant properties of organic fillers are not commonly known. This study evaluated the mechanical, thermal, and flammability properties of Ceiba petandra fiber (CPF)‐reinforced composites, which were added with snail powder (SP). To study the thermal resistance of the composite, a dynamic mechanical analyzer (DMA) and thermogravimetric analysis (TGA) were applied. SP Filler concentration increased the composite's tensile strength and thermal resistance. DMA test results showed the same trend as the composite's density value. The flame rate parameters of the composite, including the percentage of char yield and the limitation index oxygen (LOI), rose as the SP filler increased. However, the flame rate of the composite dropped because SP filler inhibited the movement of oxygen, combustible gas, and polyester degradation products. The addition of organic fillers in natural fiber composites promises better flammability and thermal resistance.

Noble metal free double helix binuclear Cu(I) complex as catalyst for carboxylative cyclization of propargylamines with atmospheric carbon dioxide toward oxazolidinones synthesis under mild conditions

The application of economically abundant, renewable and C1-feedstock, carbon dioxide into organically important compounds is considered as the hot topic in modern aspects. In this prospective, a binuclear double helix copper (I) complex (1) was synthesized through insitu reaction between quinolone-2-carbaldehyde and succino-hydrazide with copper (II) nitrate hexahydrate. Single crystal X-ray analysis of complex 1, clearly explained that both tetra co-ordinated Cu(I) ions are in tetrahedral geometric environment, having suprmolecular network arrangement via weak interaction of H-bonding, π…π stacking and CH…π stacking. The complex 1 was found to be effectively active catalyst in carboxylative cyclization reaction of propargylic amines with carbon dioxide towards oxazolidinones synthesis under atmospheric pressure and mild reaction conditions. Due to the initiation of side reaction by –NH2 group of primary propargyliamines, which reduces the yield percentage of respective oxazolidinones as a result the carboxylative cyclization reaction of primary propargylic amines, was rarely developed. In this work both primary and secondary propargylamines smoothly undergoes through this catalytic reaction and produced high percentage of desired oxazolidinones product. Again neat condition for this catalytic reaction makes the methodology more environmentally benign and sustainable. The complex 1 showed luminescent property. Moreover DFT methods were utilized in order to explain the probable mechanistic pathway of carboxylative cyclization reaction.

Outcrossing Rate and Fruit Yield of Hass Avocado Trees Decline at Increasing Distance from a Polliniser Cultivar

Optimal fruit production from many tree crops relies on the transfer of cross-pollen between trees of different cultivars rather than the transfer of self-pollen between trees of the same cultivar. However, many orchards are established with wide blocks of single cultivars, which can result in high percentages of self-fertilised fruit and sub-optimal yield and quality. We aimed to determine whether outcrossing rates and yield of Hass avocado fruit decline with increasing distance from polliniser trees of cultivar Shepard and whether selfed fruit are smaller than outcrossed fruit. Outcrossing rates declined from 49% at six trees (40 m) from a block of Shepard trees to 30% at thirty trees (160 m) from a block of Shepard trees. Tree yield across this distance declined by 44% as a result of a 69% decline in the number of outcrossed fruit per tree, without a significant decline in the number of selfed fruit per tree. Outcrossed Hass fruit were 12% heavier than selfed Hass fruit, with 3% greater diameter and 5% greater length. The study results demonstrate the importance of interplanting Type B avocado pollinisers closely with Type A Hass trees to increase fruit yield and size.

Impact of phosphorus rate, soil fumigation, and cultivar on potato yield, quality, phosphorus use efficiency and economic returns in a high phosphorus soil

Phosphorus (P) is a non-renewable resource that will become an economic limiting factor for food production in the future. Potato (Solanum tuberosum L.) is a relatively shallow rooted crop and often responds positively to P fertilizer even in soils with high soil-test P. Therefore, high-rate P fertilizers are commonly applied to produce high tuber yields in commercial potato fields. The purpose of this study was to evaluate the effects of P rate, cultivar, and soil fumigation on potato yield, quality, P uptake, P use efficiency and economic returns under high soil test P conditions. A field study with a split-plot randomized complete block design was conducted in 2021 and 2022, with fumigation treatments (no fumigant or fall-applied metam sodium) being the main plots and cultivars (Ivory Russet or Russet Burbank) as subplots. Each subplot was further divided into five sub-subplots with different P rates: 0, 37, 73, 147 and 220 kg P ha-1. The results indicated that Russet Burbank produced higher total yield than Ivory Russet. Fumigation significantly increased tuber yield and dry matter percentage. Both cultivars responded to P application and tuber P uptake increased with P rate even under very high soil test P conditions. Both partial factor productivity (PFP) and P recovery efficiency (PRE) were very low and significantly decreased with the P rate. Russet Burbank had higher PFP and PRE than Ivory Russet, and fumigated plots had significantly higher PRE than non-fumigated plots. The tuber yield output and net revenue of Russet Burbank was higher than Ivory Russet, and the highest economic return was obtained in fumigated plots at 147 kg P ha-1. These results suggest that cultivar selection and control of soil-borne diseases should be considered to optimize P input, tuber yield, and to conserve P resources.

GC-MS Analysis and Study of the Antimicrobial Activity of Citrus paradisi, Citrus aurantifolia, and Citrus sinensis Peel Essential Oils as Hand Sanitizer.

In the setting of healthcare, the use of hand sanitizers and antiseptics for hand hygiene is of paramount importance to avoid transfer of pathogenic microorganism through hand and skin contact. There is an increasing interest in the incorporation of essential oils in hand sanitizer's formula to avoid the adverse effect of conventional hand sanitizers on health. This study aimed to detect the chemical constituents of citrus peel essential oils and study their antimicrobial activity compared with commercial hand sanitizers. The qualitative and quantitative analysis of the hydrodistillated essential oils of peels of grapefruit (Citrus paradisi), lime (Citrus aurantifolia), and orange (Citrus sinensis) were carried out using gas chromatography mass spectroscopy. The disc diffusion method was used to screen the antibacterial activity of the essential oils against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, and Candida albicans compared with a 78% alcohol-based commercial hand sanitizer. The antimicrobial testing results were statistically analyzed. The highest yield percentage of the obtained essential oils was 1.09% obtained by orange oil. The GC-MS analysis indicated that monoterpene and sesquiterpene hydrocarbons occupied the largest portion of the chemical composition of the three essential oils with D-limonene as the most predominant component. All essential oils showed activity against all tested organisms. Lime essential oil showed comparable antimicrobial activity relative to the commercial 78% alcohol hand sanitizer. In conclusion, essential oils obtained from citrus fruit peel represent a rich source of compounds possessing antimicrobial properties and could be an alternative to synthetic antimicrobial agents.

Investigating bioactive phytochemicals in bulb and shoot of Allium longisepalum Bertol. from Iran

This study investigated a wide range of bioactive compounds in bulb and shoot of Allium longisepalum Bertol. from Iran. The organs were first extracted with methanol, followed by hexane, dichloromethane, chloroform, ethyl acetate, and butanol, and phytochemicals in fractions were analysed using GC/MS. Butanol exhibited the highest yield percentages in both organs. Palmitic and oleic acid were found in all fractions, while heptadecene-(8)-carboxylic acid, stearic acid, and myristic acid were detected in at least three fractions. Each fraction contained exclusively unique metabolites found only in itself. Hexane, dichloromethane, and chloroform fractions contained specific metabolites with potential antioxidant, allelopathic, antibacterial, and anti-inflammatory effects. Ethyl acetate and butanol fractions revealed significant compounds that have potential in terms of their anticarcinogenic, anti-inflammatory, hair growth-enhancing, and antimicrobial properties, in addition to containing compounds not previously identified in plants. The study analyzes bioactive compound profiles in A. longisepalum, highlighting its chemical diversity and therapeutic potential.

FORMULATION AND EVALUATION OF CIPROFLOXACIN MICROSPHERES DESIGNED BY USING NATURAL POLYMERS BY IONIC GELATION TECHNIQUE

Objective: A definitive objective for supporting drug discharge is to expand the remedial movement of the medication while limiting its incidental effects. Microspheres have become a unique medicine delivery mechanism for several disorders in this area. The popular fluoroquinolone antibiotic, Ciprofloxacin, is used to treat a variety of bacterial illnesses. This research aims to create Ciprofloxacin microspheres with sustained drug delivery using natural gum polymers. Methods: To choose and assess the ideal formulation, a variety of formulations (F1–F8) were developed. This work was completed using an innovative technology, the Ionic Gelation method. Central Composite Design (CCD) used the quadratic forward regression approach to carry out the optimization. The evaluation tests include Particle size, Scanning Electron Microscopy (SEM), FTIR, Percentage yield, Drug content, Drug Entrapment effectiveness and in vitro dissolution studies. Results: It was discovered that the best formulation was F4. From optimization, the ANOVA was found to be significant. The uneven, spherical structure of microspheres with a rough outer surface is confirmed by SEM investigation. The absence of drug-polymer interaction is confirmed by the FTIR. The formulation F4 was deemed ideal due to its high drug entrapment efficiency, drug content and maximal drug release (89.25% in 12 h). Conclusion: Due to the least plasma half-life, this drug is designed as microspheres thus maximizing the therapeutic activity and minimizing the negative effects. In this regard, microspheres have emerged as novel drug-delivery systems for various diseases. It maintains effective dose concentration, eliminates night-time dosage and decreases side effects, thus optimizing drug therapy.

Antibacterial Activities and Quantitative Phytochemical Screening of the Hexane and Ethanolic Oil Extracts of Syzygium aromaticum Against ESBL Producing Bacteria Isolates

The study was designed to determine antibacterial potentials of Syzygium aromaticum and phytochemical screening of the hexane and ethanolic oil extracts of Syzygium aromaticum (clove oil) against some multi-drug resistant bacteria (Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Shigella boydii and Klebsiella oxytoca) isolated from urine and stool samples was carried out using standard methods. Phytochemical screening revealed the presence of alkaloids, saponins, tannins, phenol, flavonoids and sterols in both oil extracts. Similarly, the percentage yield of ethanol oil extract was 34.4 with characteristic pale-brown colour and hexane percentage yield was 16.4 with characteristic pale-brown colour. The bioassay studies showed that the ethanolic oil extract exhibited an activity against Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Shigella boydii and Klebsiella oxytoca with the zone of inhibition which ranged between (10mm - 24.33mm, 9mm - 20mm, 2.33mm - 19mm, 8.33mm – 21.67mm and 8.67mm - 20.33mm) respectively for each bacterium at a concentration percent /disc... On the other hand, the hexane oil extract were found to be more active against the isolates with the following zone of inhibition which ranged between (10.27mm - 22.60mm, 13.53mm - 27.50mm, 9.34mm - 21mm, 9.10mm - 20,33mm and 4.23mm - 21.57mm) for Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Shigella boydii and Klebsiella oxytoca respectively. The results of the study prove that the ethanolic and hexane oil extracts of Syzygium aromaticum have antibacterial potentials against the tested clinical bacterial isolates, thus confirming the use of the plant for medicinal purposes.

Development of an Antihypertensive Loaded Solid Lipid Nanoparticle

Solid lipid nanoparticles were prepared by using the solvent evaporation method. The prepared solid lipid nanoparticles of felodipine from different batches were weighed accurately and filled into appropriate-sized capsules for further evaluation. The prepared nanoformulation was tested in vitro for drug entrapment efficiency found to be 97-99% for the formulation with soya lecithin and glyceryl mono-stearate and percentage yield. The results of the in vitro drug release study for all formulations performed at gastric pH 1.2 indicated that the drug was released in an immediate manner within 60 minutes, which could be due to a burst effect or a faster rate of drug release due to smaller particle size SLN13 the concentration of Glycerol Monosterate- 576.522mg and Poloxamer 188. In vitro release data from standardised nanoformulations was fitted to various release kinetics models for the drug release mechanism. The optimised formulation demonstrated improved drug release and entrapment efficiency, and in vitro release data were fitted to various kinetic models to better understand the drug release mechanism. In vitro assay results were ranged between 76.5 to 96%, Zeta potential -40.75 ± 2.33 (mV) ± SD, entrapment efficiency 99.32. Accelerated stability studies for standardised nanoformulations were performed for 6 months in accordance with standard ICH guidelines, and no appreciable changes in the physical appearance, drug content, and in vitro drug release rates of the nanoformulations were observed. As a result of the study's evidence, it is concluded that felodipineloaded solid lipid nanoparticles are the best choice for hypertension.

Evaluation of Antioxidant and Antimicrobial Activity of Ethanolic Extract of Medicinal Plants Baccaurea Ramiflora and Microcos Paniculata.

The present study was undertaken to investigate the antioxidant and antimicrobial effect of ethanolic leaf extract of Baccaurea ramiflora and Microcos paniculata. DPPH radical scavenging activity, Nitric oxide scavenging activity, Super oxide anion radical scavenging activity, Reducing power assay were used to assess antioxidant efficacy. Zone of Inhibition determination by Agar well diffusion assay was used to assess antimicrobial activity. The Baccaurea ramiflora and Microcos paniculata leaves extracted with different solvents such as petroleum ether, chloroform, ethanol and water among that in leaves ethanolic extract produce 10.78, 10.38 percentage yield respectively. Both the extracts subjected for phytochemical investigation revealed the presence of alkaloids, glycosides, tannins, saponins, proteins and flavonoids. Ethanolic extract of Baccaurea ramiflora showed maximum inhibition zone diameter was obtained in Salmonella typhi (Gram-negative bacteria) with diameter 29 mm and 25 mm respectively at 200mg/ml and 100mg/ml. Similarly, Ethanolic extract of Microcos paniculata showed minimum inhibition zone diameter compare to Baccaurea ramiflora was obtained in Salmonella typhi (Gram-negative bacteria) with diameter 23 mm and 19 mm respectively at 200mg/ml and 100mg/ml. Ethanolic extract of Baccaurea ramiflora showed maximum inhibition zone diameter was obtained in Aspergillus fumigates with diameter 25 mm and 22 mm respectively at 200mg/ml and 100mg/ml. Similarly, Ethanolic extract of Microcos paniculata showed minimum inhibition zone diameter compare to Baccaurea ramiflora was obtained in Aspergillus fumigates with diameter 21 mm and 19 mm respectively at 200mg/ml and 100mg/ml. The current findings point to Baccaurea ramiflora and Microcos paniculata antioxidant and antimicrobial properties. However future studies should be designed to isolate the active constituents responsible for the specified effect.