Lupinus Luteus Research Articles

Working collection of lupine gene pool as effective tool for use in breeding programs

Aim. To study collection accessions of the lupine gene pool for valuable economic characteristics and to build up a working collection from sources selected for the conditions of the Ukrainian Woodlands in accordance with current trends in this crop breeding. Results and Discussion. 150 accessions of the yellow lupine (L. luteus L.), 95 accessions of the white lupine (L. albus L.) and 40 accessions of the narrow-leaved lupine (L. angustifolius L.) of different eco-geographic origins from the collection of the Institute of Agricultural Microbiology and Agricultural Production of NAAS were investigated. The three-year results on 285 accessions of the lupine gene pool studied in the conditions of the Left-Bank Woodlands of Ukraine are presented. The selected sources of economically valuable traits became the basis of the lupine working collection, where traits were picked out for the regional conditions and in accordance with the current trends in this crop breeding. The collection includes 95 accessions of the yellow, white and narrow-leaved lupine species with varying expression of 11 economically valuable traits and is recommended as a genetic basis and valuable starting material for creating new, highly productive, early maturing varieties of fodder lupine. Conclusions. We built up the working collection of the lupine gene pool. There was a correlation between the performance elements (seed weight per plant, pod and seed numbers per plant) and the total yield of seeds per unit area (r = 0.520 – 0.957).

The Impact of Biopreparations and Phytopathogenic Bacteria of the Pseudomonas Genus on L-Phenylalanine Ammonia-Lyase Activity in Soybean and Lupine Plants

It is known that plant protection against diseases is based mainly on the use of pesticides. These chemicals or their degradation products have a detrimental effect on the environment and human health. Due to this, the search for methods of plant protection that are safe for the environment is becoming increasingly popular. Induction of plant resistance to disease is one of the promising non-chemical ways of protection, in which plant enzymes play a key role. It was shown that in response to pathogen invasion, plants enhance protective properties by inducing the activity of a wide range of enzymes that slow the spread of infection, in particular: peroxidases, β-1,3-glucanases, chitinases, polyphenol oxidases and L-phenylalanine ammonia-lyase (PAL). The aim of the research was to study the change in PAL activity in soybean and lupine plants under conditions of artificial infection with some phytopathogenic bacteria of the Pseudomonas genus and under the action of Ecovital and EPAA-M biological preparations. Methods. PAL activity was determined spectrophotometrically. Changes of soybean (Glycine max) and lupine (Lupinus luteus L.) plants resistance to diseases caused by bacteria of the Pseudomonas genus were evaluated using phytopathological methods. Statistical processing of the research results was performed using MS Excel computer program with Student’s t-test and estimation of least significant difference (LSD). Results. It was found that under conditions of soybean and lupine plants infection with phytopathogenic bacteria of the Pseudomonas genus on the background of pre-sowing seed treatment with Ekovital and EPAA-M biological preparations, composition based on them and synthetic standard – salicylic acid a significant increase in FAL activity in the aboveground and the degree of infectious roots – reducing of plants with pathogens was observed. It has been established that the growth of PAL activity under the condition of infection with phytopathogenic bacteria of the Pseudomonas genus occurs after 2–6 h and lasts up to 7 days from the moment of phytopathogen invasion into the plant. The most increasing of PAL activity was after treatment of seeds with Ecovital and EPAA-M composition – by 38.1–73.3% (Lupinus luteus L.) and 60.4–110% (Glycine max) compared to the control. Treatment with the composition of biological products also helped to reduce the degree of plant damage. PAL activity increasing and reducing the area of soybean and lupine leaves affected surface can indicate the induction of protective reactions in plants. Conclusions. The use of the composition of EPAA-M with the microbial preparation Ecovital contribute to the increase of PAL activity and the formation of resistance to phytopathogenic bacteria of the genus Pseudomonas in Glycine max and Lupinus luteus L. plants.

Genetic and comparative mapping of Lupinus luteus L. highlight syntenic regions with major orthologous genes controlling anthracnose resistance and flowering time

Anthracnose susceptibility and ill-adapted flowering time severely affect Lupinus luteus yield, which has high seed protein content, is excellent for sustainable agriculture, but requires genetic improvement to fulfil its potential. This study aimed to (1) develop a genetic map; (2) define collinearity and regions of synteny with Lupinus angustifolius; and (3) map QTLs/candidate genes for anthracnose resistant and flowering time. A few linkage groups/genomic regions tended to be associated with segregation distortion, but did not affect the map. The developed map showed collinearity, and syntenic regions with L. angustifolius. Major QTLs were mapped in syntenic regions. Alleles from the wild parent and cultivar, explained 75% of the phenotypic variance for anthracnose resistance and 83% for early flowering, respectively. Marker sequences flanking the QTLs showed high homology with the Lanr1 gene and Flowering-locus-T of L. angustifolius. This suggests orthologous genes for both traits in the L. luteus genome. The findings are remarkable, revealing the potential to combine early flowering/anthracnose resistant in fulfilling yield capacity in L. luteus, and can be a major strategy in the genetic improvement and usage of this species for sustainable protein production. Allele sequences and PCR-marker tagging of these genes are being applied in marker assisted selection.

Results of a study comparing planting time and biomass yield of green manure crops in the steppe zone of Mongolia

Due to the crop monoculture system which has been 60 years continuously played in a key role in the crop sector Mongolia, soil erosion and its fertility decline have been becoming one of the main pressing issues in all crop zones. The main solution of restoration of soil fertility in the steppe zone of Mongolia is not only to reduce bare fallow but also urgently need to apply green manure in all regions. Our study has focused on identifying the appropriate timing to cultivate green manure crops in the Eastern steppe region of Mongolia and to determine the convenient alternatives of crops with a high yield of biomass to use for green manure. 
 We determined the most convenient time to use crop biomass for green manure is between 22-27th.July for the green manure crops cultivated on the 20th of May.
 For the crops cultivated on the 30th of May green manure time can be determined between 2-6th. August and for the crops cultivated on the 10th of June the convenient time would be between 10-13th.August. 
 We prioritized the best option of the crop alternatives based on the 3 years average yield of green mass and concluded that field pea and oat mixture, Sudan grass, and fodder pea mixture can be one of the convenient options for green manure in the steppe zone of Mongolia. According to our study results, convenient alternatives with the highest root mass were yellow clover (27.5-60.5c/ha) and white lupine (27.5-61.4c/ha). 
 In terms of biomass, the convenient alternatives with the highest biomass were the following crops planted on the 20th of May such as mixture of field pea and oat (82.5-112.2c/ha), fodder pea and Sudan grass (67.4-97.0c/ha), as well as Sudan grass (57.1-110.0c/ha. It is considered that in the steppe zone green manure crops can be used for green manure if the biomass of the crops can exceed 40c/ha. [1] The appropriate timing of the planting period should not be later than the 30th of May.

Proximate composition, lipid and phenolic profiles, and antioxidant activity of different ecotypes of Lupinus albus, Lupinus luteus and lupinus angustifolius

Lupine seed represents an interesting, non-genetically modified, and low cost alternative to soybean as an important source of protein and oil. In this study, we compare seed protein, mineral, fiber and starch content, lipid and phenolic profile, and antioxidant properties of three lupine wild species (Lupinus albus, Lupinus luteus, and lupinus angustifolius). For each lupine species, nutritional composition of different Tunisian ecotypes was compared to determine the influence of geographical origin on these compounds. Standard protocols and ultra-high‐performance liquid chromatography/tandem mass spectrometry methods were used. Results revealed that several compounds showed a significant difference between species as well as between local ecotypes and commercialized variety. L. albus seeds were shown characterized by the highest protein and oleic acid content and the lowest starch content. Results revealed that lipids and phenolics were highly influenced by the ecotype factor. Large differences were found between L. luteus ecotypes for oleic acid and linoleic acid content, ranging from 164.97 to 302.73 mg kg−1 DM and from 306.03 to 616.97 mg kg−1 DM, respectively. Little amount of miristoleic acid, lignoceric acid, and margaric acid were found in lupine seeds for the first time. For phenolic profile, interestingly to highlight for the first time the presence of morin dihydrate among lupines species, especially in L. tbk2 which showed the highest antioxidant activity.

Natural alkaloids from narrow-leaf and yellow lupins transfer to soil and soil solution in agricultural fields

BackgroundLupin is a promising legume crop, belongs to the Fabaceae (or Leguminosae) family. Lupin production for traditional and functional foods or animal feed is limited, due to the content of toxic quinolizidine (QA)s and indole alkaloids (IA)s. These compounds may not only pose a risk to humans and animals through food consumption, but may also affect soil and aquatic ecosystems. Field experiments were conducted to study the alkaloids content in both narrow-leaved or blue (L. angustifolius) and yellow (L. luteus) lupin plant tissue during a full growing season and understand the environmental fate of alkaloids in soil and water. Suction cups were used to collect soil pore water (soil solution) at four depths: 10, 25, 50 and 70 cm. A full protocol for sample preparation and UPLC–MS/MS quantification of alkaloids in plant, soil and water was developed.ResultsDuring the field experiments the alkaloids in the plant tissues increased, at the harvest stage the content was highest with 21.4 and 24.6 mg/kg dry weight (dw) for blue and yellow lupin, respectively. In soil, alkaloids quantified during the growing season (max concentration was 1.3 × 102 µg/kg dw) and even detected after harvest (0.2 µg/kg dw). In soil pore water samples, alkaloids were not detected during summer, but the concentrations increased to 9.8 × 102 and 1.5 × 103 ng/L for blue and yellow, respectively, in September when autumn precipitation began.ConclusionsThe results show the amount of alkaloids transferred from plant tissue into soil and soil pore water estimated to be on average 0.016% and 0.005% in soil and soil pore water, respectively. Alkaloids leached from topsoil to subsoil layers; the concentrations decline with soil depth. This study demonstrates that alkaloids are mobile compounds in the soil environments, thus lupin production may affect soil or aquatic ecosystems, and reduce water quality.

The Role of Saccharides in the Mechanisms of Pathogenicity of Fusarium oxysporum f. sp. lupini in Yellow Lupine (Lupinus luteus L.)

The primary aim of this study was to determine the relationship between soluble sugar levels (sucrose, glucose, or fructose) in yellow lupine embryo axes and the pathogenicity of the hemibiotrophic fungus Fusarium oxysporum f. sp. Schlecht lupini. The first step of this study was to determine the effect of exogenous saccharides on the growth and sporulation of F. oxysporum. The second one focused on estimating the levels of ergosterol as a fungal growth indicator in infected embryo axes cultured in vitro on sugar containing-medium or without it. The third aim of this study was to record the levels of the mycotoxin moniliformin as the most characteristic secondary metabolite of F. oxysporum in the infected embryo axes with the high sugar medium and without it. Additionally, morphometric measurements, i.e., the length and fresh weight of embryo axes, were done. The levels of ergosterol were the highest in infected embryo axes with a sugar deficit. At the same time, significant accumulation of the mycotoxin moniliformin was recorded in those tissues. Furthermore, it was found that the presence of sugars in water agar medium inhibited the sporulation of the pathogenic fungus F. oxysporum in relation to the control (sporulation of the pathogen on medium without sugar), the strongest inhibiting effect was observed in the case of glucose. Infection caused by F. oxysporum significantly limited the growth of embryo axes, but this effect was more visible on infected axes cultured under sugar deficiency than on the ones cultured with soluble sugars. The obtained results thus showed that high sugar levels may lead to reduced production of mycotoxins by F. oxysporum, limiting infection development and fusariosis.

Drought Disrupts Auxin Localization in Abscission Zone and Modifies Cell Wall Structure Leading to Flower Separation in Yellow Lupine.

Drought causes the excessive abscission of flowers in yellow lupine, leading to yield loss and serious economic consequences in agriculture. The structure that determines the time of flower shedding is the abscission zone (AZ). Its functioning depends on the undisturbed auxin movement from the flower to the stem. However, little is known about the mechanism guiding cell–cell adhesion directly in an AZ under water deficit. Therefore, here, we seek a fuller understanding of drought-dependent reactions and check the hypothesis that water limitation in soil disturbs the natural auxin balance within the AZ and, in this way, modifies the cell wall structure, leading to flower separation. Our strategy combined microscopic, biochemical, and chromatography approaches. We show that drought affects indole-3-acetic acid (IAA) distribution and evokes cellular changes, indicating AZ activation and flower abortion. Drought action was manifested by the accumulation of proline in the AZ. Moreover, cell wall-related modifications in response to drought are associated with reorganization of methylated homogalacturonans (HG) in the AZ, and upregulation of pectin methylesterase (PME) and polygalacturonase (PG)—enzymes responsible for pectin remodeling. Another symptom of stress action is the accumulation of hemicelluloses. Our data provide new insights into cell wall remodeling events during drought-induced flower abscission, which is relevant to control plant production.

Effect of fungicides on the enzymatic activity of the antioxidant system and the chlorophyll content in lupine plants during seed dressing

Goal. The effect of fungicides on the dynamics of the activity of peroxidase, catalase (CAT) and chlorophyll content in lupine plants during seed dressing has been established.
 Methods. Laboratory and vegetation researches were conducted in the laboratory of analytical chemistry of pesticides of the Institute of Plant Protection. Yellow lupine (Lupinus luteus L.), variety Obriy has been grown. The objects of research were fungicides triticonazol (40 g/t) and its combination with prochloraz (120 g/t). Determination of the content of fungicides in plants was carried out using chromatographic methods according to officially approved methods and me­thods developed in the laboratory of analytical chemistry of pesticides. Chlorophyll content and peroxidase activity were measured by colorimetric method, catalase activity — by titrimetric method.
 Results. According to the research results, the varying sensitivity of the enzymatic system of antioxidant defense (catalase, peroxidase) in response to seed dressing by fungicides was recorded. It showed that on the 10th day after sowing, content of triticonazol in plants was 0.8 mg/kg, and the peroxidase activity was similar to that in untreated plants. Subsequently, against the background of a decrease in the content of the active substance, a gradual activation of the enzyme was observed. Catalase activity also gradually increased beginning from the 14th day, and on the 30th day it exceeded the corresponding control indicator by 40%. When using a combination of triticonazol with prochloraz, the disturbance in the balance of peroxidase catalase was more significant. However, by the phase of 7—8 leaves, with a minimal total content of fungicides (0.38 mg/kg), the enzyme activity approached the control level, which is associated with the restoration of plant homeostasis and the formation of its adaptive potential under stress conditions. The stimulating effect of these fungicides on chlorophyll content at the initial growth stages of lupine was established. The chlorophyll concentration in fungicides-treated plants exceeded the control indicator by 11—29%.
 Conclusions. The use of systemic triazole fungicides to protect seedlings, improves the photosynthetic activity of plants and at the same time acts as a stress factor that activates protecting enzymes (catalase, peroxidase), which trigger the development of protective adaptive reactions of plants.

The effect of various protein sources in goose diets on meat quality, fatty acid composition, and cholesterol and collagen content in breast muscles

Goose meat is characterized by good quality and a good fatty acid composition. Geese do not need many compounds in their feed to ensure suitable growth. The main source of protein in most feed is soybean meal (SBM). New cultivars of yellow lupin with fewer antinutrients could be a substitute for soybean. The study's aim was to compare the quality of the carcass and meat, as well as the fatty acid composition and the collagen and cholesterol content in the breast muscles from geese fed a diet based on yellow lupin as an replacement for soybean meal. In the study, geese were divided into 2 study groups. The first was a control group (Group 1), fed a feed based on SBM; the second one was an experimental group (Group 2), fed a feed based on yellow lupin “Mister”, potato protein, and brewer's yeast. The rearing period was divided into 3 stages, and the last stage was oat fattening. Each group (105 birds in each group) was divided into 5 replications with 21 birds. After 16 wk of rearing, 10 geese from each group were slaughtered. The carcasses were analyzed for physicochemical traits (dissection, color, water-holding capacity, and chemical composition of the breast and leg muscles, as well as pH level, drip loss, and fatty acid profile of the breast muscles). The drip loss from the breast muscles was higher (P < 0.05) and the water-holding capacity of the leg muscles lower (P < 0.05) in Group 1 than in Group 2. Group 1 also displayed a higher content of protein and water in breast and leg muscles (P < 0.05) but lower fat content than that of Group 2 (P < 0.05). The linoleic acid content of the breast muscles was higher in Group 2 (P < 0.05), whereas the other fatty acid levels were comparable between the groups. The total content of polyunsaturated fatty acid (PUFA) (n-6 and n-3) and the PUFA/ saturated fatty acid ratio was higher in Group 2 (P < 0.05). Moreover, the thrombogenic index was lower (P < 0.05) in the geese fed a lupin-diet. Overall, the yellow lupin–based feed had beneficial effects on the goose meat's traits, and it can be used as a high-protein compound in diets for geese. It is also possible to produce traditional geese fattened by oats.

Identification of the endosymbionts from Sulla spinosissima growing in a lead mine tailings in Eastern Morocco as Mesorhizobium camelthorni sv. aridi.

To identify the bacteria nodulating Sulla spinosissima growing profusely in a lead and zinc mine tailings in Eastern Morocco. In all, 32 rhizobial cultures, isolated from root nodules of S. spinosissima growing in soils of the mining site, were tolerant to different heavy metals. The ERIC-polymerase chain reaction (PCR) fingerprinting analysis clustered the isolates into seven different groups, and the analysis of the 16S rRNA sequences of four selected representative strains, showed they were related to different species of the genus Mesorhizobium. The atpD, glnII and recA housekeeping genes analysis confirmed the affiliation of the four representative strains to Mesorhizobium camelthorni CCNWXJ40-4T , with similarity percentages varying from 96·30 to 98·30%. The sequences of the nifH gene had 97·33-97·78% similarities with that of M. camelthorni CCNWXJ40-4T ; however, the nodC phylogeny of the four strains diverged from the type and other reference strains of M. camelthorni and formed a separated cluster. The four strains nodulate also Astragalus gombiformis and A. armatus but did not nodulate A. boeticus, Vachellia gummifera, Prosopis chilensis, Cicer arietinum, Lens culinaris, Medicago truncatula, Lupinus luteus or Phaseolus vulgaris. Based on similarities of the nodC symbiotic gene and differences in the host range, the strains isolated from S. spinosissima growing in soils of the Sidi Boubker mining site may form a different symbiovar within Mesorhizobium for which the name aridi is proposed. In this work, we show that strains of M. camelthorni species nodulating S. spinosissima in the arid area of Eastern Morocco constitute a distinct phylogenetic clade of nodulation genes; we named symbiovar aridi, which encompasses also mesorhizobia from other Mediterranean desert legumes.

Effects of legume-diet and sex of ducks on the growth performance, physicochemical traits of meat and fatty acid composition in fat

Duck meat enjoys growing popularity among consumers. Alternative protein sources to soybean are being investigated to eliminate genetically modified components from the poultry’ diet. The aim of this study was to compare growth performance, quality of meat, and fatty acid composition in subcutaneous and abdominal fat from ducks fed a diet based on yellow lupin and rapeseed meal, sources of protein alternative to soybean meal (SBM). Ducks were allocated to different dietary treatment groups and reared for 8 weeks (N = 102 per group). Group A received a diet based on SBM, while group B was fed a diet based on yellow lupin with the addition of rapeseed meal. Both groups were divided into two subgroups, of male and female birds. Growth performance parameters and zoometric traits of ducks were monitored during the growth period. After 8 weeks selected birds were slaughtered and dissected (N = 10 per group). Carcass composition was calculated and selected traits of meat quality important for further processing were analysed. Subcutaneous and abdominal fat were collected to analyse fatty acid composition. The alternative diet had no negative effect on ducks’ growth performance parameters and dressing percentage. The replacement of SBM with yellow lupin and rapeseed meal increased n-3 fatty acid content, which is important for consumers. In conclusion, SBM can be replaced with feed containing 60.10% of yellow lupin and 14.00% of rapeseed meal in concentrate. These sources of protein are mainly recommended for small poultry farms, which do not always have access to SBM and prepare poultry feed from their own crops.

Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.

1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks. From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain. Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy. Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds. The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed. The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2). The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes. We anticipate our approach to be a robust assessment method potentially applicable to biological extracts containing NAPE species and can provide comprehensive profiles and contents.

Phenotypic characterisation and linkage mapping of domestication syndrome traits in yellow lupin (Lupinus luteus L.)

The transformation of wild plants into domesticated crops usually modifies a common set of characters referred to as ‘domestication syndrome’ traits such as the loss of pod shattering/seed dehiscence, loss of seed dormancy, reduced anti-nutritional compounds and changes in growth habit, phenology, flower and seed colour. Understanding the genetic control of domestication syndrome traits facilitates the efficient transfer of useful traits from wild progenitors into crops through crossing and selection. Domesticated forms of yellow lupin (Lupinus luteus L.) possess many domestication syndrome traits, while their genetic control remains a mystery. This study aimed to reveal the genetic control of yellow lupin domestication traits. This involved phenotypic characterisation of those traits, defining the genomic regions controlling domestication traits on a linkage map and performing a comparative genomic analysis of yellow lupin with its better-understood relatives, narrow-leafed lupin (L. angustifolius L.) and white lupin (L. albus L.). We phenotyped an F9 recombinant inbred line (RIL) population of a wide cross between Wodjil (domesticated) × P28213 (wild). Vernalisation responsiveness, alkaloid content, flower and seed colour in yellow lupin were each found to be controlled by single loci on linkage groups YL-21, YL-06, YL-03 and YL-38, respectively. Aligning the genomes of yellow with narrow-leafed lupin and white lupin revealed well-conserved synteny between these sister species (76% and 71%, respectively). This genomic comparison revealed that one of the key domestication traits, vernalisation-responsive flowering, mapped to a region of conserved synteny with the vernalisation-responsive flowering time Ku locus of narrow-leafed lupin, which has previously been shown to be controlled by an FT homologue. In contrast, the loci controlling alkaloid content were each found at non-syntenic regions among the three species. This provides a first glimpse into the molecular control of flowering time in yellow lupin and demonstrates both the power and the limitation of synteny as a tool for gene discovery in lupins.

Response of Yellow Lupine to the Proximity of Other Plants and Unplanted Path in Strip Intercropping

Taking into account the climatic conditions of central Europe, yellow lupine is often considered as an alternative to soybean, which has significantly higher thermal requirements. Attempts to intercrop yellow lupine with cereals have often resulted in failure. In combined production, the relative amount of lupine has proven to be considerably smaller given the sowing mix proportions and its yield potential in pure stand. Low yield is attributed to lupine’s low competitive potential, therefore strip intercropping presents a viable alternative. The main goal of the experiment was to determine the response of yellow lupine to the neighboring presence of wheat, triticale, barley, and pea, as well as to estimate the production effects of lupine in strip intercropping. Field trials were carried out in Poland (53°13′ N; 17°51′ E) in the years 2008–2010. The experimental factor consisted of row layout: a four-row separation between lupine and the neighboring species. The proximity of cereals and peas proved to be most unfavorable to yellow lupine. It was determined that yellow lupine was most intolerant of barley and least affected by the proximity of peas. Depending on the neighboring species, adverse effects extended up to the third row of lupine’s canopy. A beneficial alternative for the production effect involves an introduction of a path separating the lupine strip from the tested species.

The Effect of Diet Based on Legume Seeds and Rapeseed Meal on Pig Performance and Meat Quality.

Simple SummaryPig diets are primarily composed of cereal-based ingredients and contain soybean meal as the main protein source. A growing trend for increased protein requirements has been noted in animal nutrition. The price of extracted soybean meal fluctuates significantly, hence it is worth replacing it with mixtures based on domestic sources of protein. One of the factors that have a direct impact on the quality of meat is animal nutrition. The aim of this work was to assess the impact of a total dietary replacement of extracted soybean meal (SBM) on the body weight gain and pork quality. The fattening pigs, which were offered diets based on SBM, had similar raw meat parameters as the pigs which were fed diets based on legumes and rapeseed as protein sources.The aim of this work was to assess the impact of a total dietary replacement of extracted soybean meal (SBM) on body weight gain and pork quality. DanBred hybrid piglets were divided into four groups of 10 piglets each. Groups I (males) and II (females) were the control groups and fed a standardized SBM-based complete feed. The experimental groups III (males) and IV (females) were offered a diet in which the SBM was replaced with extracted rapeseed meal (RSM) and legume plants (pea and yellow lupin). After 83 days of fattening, the animals were slaughtered. Based on the collected data, the daily weight gain (DWG), feed intake (FI), and feed conversion ratio (FCR) were calculated. In addition, longissimus dorsi muscle was subjected to physicochemical analyses, including the basic chemical composition. All the analyses were performed in accordance with the applicable methodologies. As a result of this experiment, no interactions were found between the experimental factors (sex and diet). The replacement of SMB by legumes and RSM resulted in a significant reduction in the final body weight of growing–finishing pigs. Additionally, daily body weight gain was reduced between 35–83 days, and through the whole fattening period (0–83 days). Most pork meat quality parameters were not affected by the type of mix feed and sex (p > 0.05). The fattening pigs that were fed legume seeds and RSM had significantly reduced fatness (p > 0.05) compared to the control pigs. In males, there was also a significantly lower pH45 (p < 0.05). It can be concluded that feeding pigs with diets containing legume plants and extracted rapeseed meal does not affect the pork meat quality, but it may worsen the body weight gain.

The Role of Sugars in the Regulation of the Level of Endogenous Signaling Molecules during Defense Response of Yellow Lupine to Fusarium oxysporum.

Soluble sugars such as sucrose, glucose and fructose in plant host cells not only play the role as donors of carbon skeletons, but they may also induce metabolic signals influencing the expression of defense genes. These metabolites function in a complex network with many bioactive molecules, which independently or in dialogue, induce successive defense mechanisms. The aim of this study was to determine the involvement of sucrose and monosaccharides as signaling molecules in the regulation of the levels of phytohormones and hydrogen peroxide participating in the defense responses of Lupinus luteus L. to a hemibiotrophic fungus Fusarium oxysporum Schlecht f. sp. lupini. A positive correlation between the level of sugars and postinfection accumulation of salicylic acid and its glucoside, as well as abscisic acid, was noted. The stimulatory effect of sugars on the production of ethylene was also reported. The protective role of soluble sugars in embryo axes of yellow lupine was seen in the limited development of infection and fusariosis. These results provide evidence for the enhanced generation of signaling molecules both by sugar alone as well as during the crosstalk between sugars and infection caused by F. oxysporum. However, a considerable postinfection increase in the level of these signaling molecules under the influence of sugars was recorded. The duration of the postinfection generation of these molecules in yellow lupine was also variable.

Disruption of the Auxin Gradient in the Abscission Zone Area Evokes Asymmetrical Changes Leading to Flower Separation in Yellow Lupine.

How auxin transport regulates organ abscission is a long-standing and intriguing question. Polar auxin transport across the abscission zone (AZ) plays a more important role in the regulation of abscission than a local concentration of this hormone. We recently reported the existence of a spatiotemporal sequential pattern of the indole-3-acetic acid (IAA) localization in the area of the yellow lupine AZ, which is a place of flower detachment. In this study, we performed analyses of AZ following treatment with an inhibitor of polar auxin transport (2,3,5-triiodobenzoic acid (TIBA)). Once we applied TIBA directly onto the AZ, we observed a strong response as demonstrated by enhanced flower abscission. To elucidate the molecular events caused by the inhibition of auxin movement, we divided the AZ into the distal and proximal part. TIBA triggered the formation of the IAA gradient between these two parts. The AZ-marker genes, which encode the downstream molecular components of the inflorescence deficient in abscission (IDA)-signaling system executing the abscission, were expressed in the distal part. The accumulation of IAA in the proximal area accelerated the biosynthesis of abscisic acid and ethylene (stimulators of flower separation), which was also reflected at the transcriptional level. Accumulated IAA up-regulated reactive oxygen species (ROS) detoxification mechanisms. Collectively, we provide new information regarding auxin-regulated processes operating in specific areas of the AZ.

LuluDB-The Database Created Based on Small RNA, Transcriptome, and Degradome Sequencing Shows the Wide Landscape of Non-coding and Coding RNA in Yellow Lupine (Lupinus luteus L.) Flowers and Pods.

Yellow lupine (Lupinus luteus L.) belongs to a legume family that benefits from symbiosis with nitrogen-fixing bacteria. Its seeds are rich in protein, which makes it a valuable food source for animals and humans. Yellow lupine is also the model plant for basic research on nodulation or abscission of organs. Nevertheless, the knowledge about the molecular regulatory mechanisms of its generative development is still incomplete. The RNA-Seq technique is becoming more prominent in high-throughput identification and expression profiling of both coding and non-coding RNA sequences. However, the huge amount of data generated with this method may discourage other scientific groups from making full use of them. To overcome this inconvenience, we have created a database containing analysis-ready information about non-coding and coding L. luteus RNA sequences (LuluDB). LuluDB was created on the basis of RNA-Seq analysis of small RNA, transcriptome, and degradome libraries obtained from yellow lupine cv. Taper flowers, pod walls, and seeds in various stages of development, flower pedicels, and pods undergoing abscission or maintained on the plant. It contains sequences of miRNAs and phased siRNAs identified in L. luteus, information about their expression in individual samples, and their target sequences. LuluDB also contains identified lncRNAs and protein-coding RNA sequences with their organ expression and annotations to widely used databases like GO, KEGG, NCBI, Rfam, Pfam, etc. The database also provides sequence homology search by BLAST using, e.g., an unknown sequence as a query. To present the full capabilities offered by our database, we performed a case study concerning transcripts annotated as DCL 1–4 (DICER LIKE 1–4) homologs involved in small non-coding RNA biogenesis and identified miRNAs that most likely regulate DCL1 and DCL2 expression in yellow lupine. LuluDB is available at http://luluseqdb.umk.pl/basic/web/index.php.

Growth performance and Carcass quality in broiler chickens fed on legume seeds and rapeseed meal.

Simple SummaryThe production of broiler chickens is a dynamically developing industry. Most feeds are based on soybean meal as a source of protein. However, new challenges are arising in the meat production market, and consumers expect a wider range of products to choose from. There is potential in the production of broiler chickens fed on other sources of protein, e.g., legume seeds and rapeseed meal. The aim of this study was to investigate whether other sources of protein give similar results in terms of growth performance and meat quality compared to soybean meal diets. It was shown that the use of legume seeds and rapeseed meal did not affect most of the carcass characteristics and meat quality. However, the growth performance results were lower than when using soybean meal. Because they contained the smallest amount of antinutritional substances, faba beans are proposed as a possible partial replacement for soybean meal.The aim of this study was to compare the growth performance parameters, carcass quality, and meat traits in broiler chickens fed on diets containing legume seeds and rapeseed meal as an alternative to soybean meal. In this study, 448 male ROSS 308 chicks were divided into subgroups: a control group (I) fed on soybean meal (SBM), and six experimental groups II—rapeseed meal (RSM); III—white lupin (WY); IV—yellow lupin (YL); V—narrow-leaved lupin NLL; VI—pea (Pe); and VII—faba bean (FB). After 42 days of rearing, 10 birds from each group were slaughtered and dissected. The control group was characterized by better growth performance compared to the other groups. In addition, the European Broiler Index was lower in each experimental group compared to the SBM group. A lower dressing percentage was found only in the NLL group. The muscle content in birds from the RSM and FB groups was significantly higher than in the other groups, but the fat content was lower. Meat from SBM group was characterized by the highest protein content, but a reduced content of fat and water in the muscles. The most similar results were found between the control group and the FB group receiving a diet based on faba beans. Furthermore, lupins had a similar effect on the carcass traits when used in the diets. The quality of meat in broilers fed on faba beans with the addition of potato protein and brewers’ yeast was similar to that of those fed on soybean meal, because the antinutrients were the lowest in faba bean seeds. Faba beans are proposed as a possible alternative source of protein in poultry diets. Other legume seeds should be analyzed in future studies.