Abstract
Anthracnose susceptibility and ill-adapted flowering time severely affect Lupinus luteus yield, which has high seed protein content, is excellent for sustainable agriculture, but requires genetic improvement to fulfil its potential. This study aimed to (1) develop a genetic map; (2) define collinearity and regions of synteny with Lupinus angustifolius; and (3) map QTLs/candidate genes for anthracnose resistant and flowering time. A few linkage groups/genomic regions tended to be associated with segregation distortion, but did not affect the map. The developed map showed collinearity, and syntenic regions with L. angustifolius. Major QTLs were mapped in syntenic regions. Alleles from the wild parent and cultivar, explained 75% of the phenotypic variance for anthracnose resistance and 83% for early flowering, respectively. Marker sequences flanking the QTLs showed high homology with the Lanr1 gene and Flowering-locus-T of L. angustifolius. This suggests orthologous genes for both traits in the L. luteus genome. The findings are remarkable, revealing the potential to combine early flowering/anthracnose resistant in fulfilling yield capacity in L. luteus, and can be a major strategy in the genetic improvement and usage of this species for sustainable protein production. Allele sequences and PCR-marker tagging of these genes are being applied in marker assisted selection.
Highlights
Food security, soil fertility and sustainable food production can be significantly improved by the greater use and improvement of various grain legumes[1] and especially Lupinus spp., which have been traditionally used in human and animal diets[2,3]
No gene or markers associated with anthracnose resistance have been reported, and only recently some flowering time QTLs have been identified in L. luteus[24]
Synteny has been reported between the reference genome of L. angustifolius, with L. albus, and other model legume species[26,29,31], which highlights the possibility of synteny and collinearity with L. luteus, since both species have been reported to share the same lineage and clade[11]
Summary
Soil fertility and sustainable food production can be significantly improved by the greater use and improvement of various grain legumes[1] and especially Lupinus spp., which have been traditionally used in human and animal diets[2,3]. Anthracnose resistance is critical to improve yield in lupin, the disease has become a serious problem worldwide, causing significant yield losses, as high as 100%, and a major limiting factor for lupin production It is caused by Colletotrichum lupini (Bondar)[19,20]. Another essential trait in the crop adaptation processes of L. luteus is flowering time, where it is important to breed germplasm suited to specific environmental conditions, directly improving yield. Greater progress had been achieved in L. angustifolius, which has been proposed as a reference genome in comparative studies[25,26] In this species, the cultivar Tanjil, has been widely used for breeding anthracnose resistance. Synteny has been reported between the reference genome of L. angustifolius, with L. albus, and other model legume species[26,29,31], which highlights the possibility of synteny and collinearity with L. luteus, since both species have been reported to share the same lineage and clade[11]
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
