The article presents the study results of the breeding value of winter triticale lines in the main competitive variety-testing. The variety “Student” was used as a standard variety introduced into the State List of Protected Breeding Achievements in the Nizhny-Volga region. There have been selected the most valuable lines as the sources for use in breeding programs. There were identified such promising lines for high grain productivity as № 1 (F11Polessky 10/Vodoley), № 7 (F12Sargau/Polessky 7), № 9 (F8Vodoley/ADP-2//Modus), № 12 (F7Student/Vodoley) with potential of 65.5–83.0 hwt/ha. The plant height of these lines is reduced compared with the standard variety on 22–37 cm. The study results have shown the existence of a wide spectrum of genotypic variability of the “cell membrane permeability”, as a key physiological indicator of drought resistance among the triticale lines and varieties. According to the damage degree of cell membranes, the line № 9 and the variety “Svyatozar” were selected. The lowest value of this indicator was revealed in the genotypes (35.5 and 31.5%) and it does not differ from that in the winter rye variety “Saratovskaya 7” (33.5%, 13.1% of НСР05). The lines № 1, № 7, № 9 and № 11, which according to the complex of the studied parameters are close to the variety “Valentin 90”, are of interest in the breeding work to improve baking traits of triticale.