Treatment Of Joint Diseases Research Articles

The effect of the 2-UPS/RR ankle rehabilitation robot with coupling biomechanical model on muscle behaviors.

With the popularization of biomechanical simulation technology, aiming at the rehabilitation of ankle joint injury, we imported simplified model of proposed 2-UPS/RR (two identical unconstraint kinematic branches with a universal-prismatic-spherical (UPS) structure and two rotating pair (R)) ankle rehabilitation robot into AnyBody Modeling System. Therefore, a human-machine model was established using the HILL-type muscle model and muscle recruitment criteria. This paper investigated the effects of rehabilitation trajectories on biomechanical response during rehabilitation. Additionally, three main lower limb muscles (soleus, peroneal brevis, and extensor digitorum longus) were examined under different rehabilitation trajectories (plantar dorsiflexion, varus or valgus, and compound movement) in the present study. Based on the biomechanical response of lower limbs, the results showed that different muscles had different sensitivities to the change of rehabilitation trajectories. The correlation coefficient between joint force and plantar dorsiflexion angle reached 0.99 (P < 0.01), indicating that the change of joint force was mainly dominated by plantar dorsiflexion/plantar flexion, but also affected by varus or valgus. Safe rehabilitation training can be achieved by controlling the designed 2-UPS/RR rehabilitation robot. The behavior of muscle force and joint force under different rehabilitation trajectories can meet the needs of rehabilitation and treatment of joint diseases, and provide more reasonable suggestions for early rehabilitation.

Intra-articular delivery of antioxidative polymer-based nanospheres reduces intracellular reactive oxygen species in macrophages and alleviates cartilage damage in a rat model.

Cartilage damage is frequent in various joint diseases, mainly manifested by the loss of type II collagen and the degradation of proteoglycans. Diclofenac sodium is a commonly used drug for the treatment of joint diseases, but simple administration is often affected by drug clearance and rapid metabolism. Intra-articular drug delivery is an effective method for local enrichment of high concentration of drugs. However, due to the short half-life of diclofenac sodium, prolonging the stability and duration of the drug can alleviate the disadvantages of direct intra-articular application. Nanospheres for delivering drugs to treat joint diseases could be a remedy for cartilage damage. In addition, excessive production of reactive oxygen species (ROS) by macrophages activated in damaged cartilage would aggravate cartilage damage. Therefore, this study intends to use poly lactic-co-glycolic acid nanospheres to load and deliver diclofenac sodium to inhibit chondrocyte death while regulating the generation of ROS, thereby promoting the treatment of cartilage damage.

Molecular Mechanisms of Action of Undenatured Type II Collagen: Experimental and Clinical Evidence

In the treatment of joint diseases, including osteoarthritis (OA), the use of standardized extracts of undenatured type II collagen (UDC-II) is promising. It is known that UDC-II is involved in the regulation of innate and adaptive immunity (reduction of autoimmune reactions that stimulate cartilage degradation) and in the reduction of chronic inflammation activity (modulation of cytokines and prostaglandins). The effect of UDC-II on discoidin receptors of chondrocytes helps to prevent structural disorders of the cartilage connective tissue. Experimental and clinical studies have shown that under the influence of standardized UDC-II, there is an increase in the proportion of regulatory CD4+ T cells, a decrease in the levels of pro-inflammatory cytokines, such as interleukin (IL) 1β, IL6, tumor necrosis factor α, CRP, prostaglandins in the blood, as well as matrix metalloproteinase 3 and NF-κB expression in cartilage. The use of UDC-II in OA leads to a significant reduction in pain, an increase in the range of joint motion, an improvement in joint function according to WOMAC and quality of life.

Molecular dynamics simulation of hyaluronic acid hydrogels: Effect of water content on mechanical and tribological properties

Background and objectiveRecently conducted biomedical studies have shown that the drug diffusivity of hyaluronic acid hydrogel plays an important role in the treatment of joint diseases. The drug diffusivity is closely related to the water content of hydrogel. In addition, different water content will not only affect its mechanical and tribological properties, but also change the effect of drug release. MethodsIn this work, a Molecular dynamics simulation was used to investigate the effect of water content on spatial distribution, tribological and mechanical properties of a hyaluronic acid hydrogel network. This paper focuses on the analysis and calculation of the radial distribution function of 20, 40, 60, and 80% water content model and the friction force and mechanical parameters under the influence of different load and friction speed. ResultsThe results show that at 20 and 40% water content, the spatial distribution is loose and the intermolecular force is not strong, resulting in a major lack in tribological and mechanical properties; whereas at 60 and 80% water content, the spatial distribution becomes gradually compact and the intermolecular force is gradually increased. The tribological and mechanical properties manifest a marked improvement. ConclusionsThe calculations reveal that the hydrogel model has the best wear resistance, pressure resistance, and plastic deformation resistance at 80% water content. In the range of 20–80% water content, the mechanical properties and friction properties of hydrogels become better and better with the increase of water content.

Nimbolide loaded sustained release microparticles as single-dose formulations for effective management of arthritis

At present, sustained-release formulations have gained immense interest in drug development for improving the pharmacokinetic and pharmacodynamic properties of drugs. Nimbolide (NMB), one of the active constituents of the Neem tree (Azadirachta indica), has been studied for its anti-carcinogenic, anti-fibrotic, and anti-inflammatory properties, but it has poor pharmacokinetic issues. This study aims for preparing an intra-articularly injectable NMB-PLGA microparticle formulation (NMB MPs) that releases NMB in a sustained fashion once localized in joints for the treatment of arthritis. The formulation was characterized by scanning electron microscope, infrared spectroscopy, and in vitro drug release profiles. The MPs were spherical as evidenced by microscopic images with a mean diameter of 9.17 ± 2.75 μm. With the drug loading of 28% of NMB, the entrapment efficiency was 71.22 ± 3.53%. The formulated MPs could extend the drug release up to 62.25 ± 6.58%in 33 days in the in vitro conditions. The in vivo therapeutic effect of NMB MPs (single dose) was investigated in comparison to NMB free drug (weekly doses) in the rat model of Complete Freund's Adjuvant (CFA) induced arthritis. The anti-arthritic activity was evaluated based on knee swelling, X-ray analysis of knee joints, histopathology scoring, and expression of various essential proteins. The observed superior protective effects can be attributed to the sustained release of NMB from NMB MPs that facilitated the maintenance of therapeutic drug levels. In conclusion, NMB MPs can be a useful injectable delivery system for enhancing therapeutic efficiency in treating joint diseases.

The Equine Temporomandibular Joint: Comparisons Between Standard and Needle Arthroscopic Examination of Cadaver Specimens and Standing Horses.

Background:Definitive diagnosis of equine temporomandibular joint osteoarthritis (TMJ-OA) may require advanced diagnostic imaging. Arthroscopy is a modern, minimally invasive, diagnostic, and treatment modality. Standing arthroscopic treatment of joint disease is a relatively recent advance in equine surgery, despite which there are few published comparisons between the available arthroscopic systems.ObjectiveTo compare and contrast two arthroscopic systems for assessing the equine temporomandibular joint compartments in cadavers and standing horses.Study designExperimental study.MethodsPhase I involved the assessment of the discotemporal joint (DTJ) and discomandibular (DMJ) joint compartments of both temporomandibular joints (TMJ) of 14 cadaveric equine heads using a caudally placed arthroscopy portal. Joints were initially examined using the needle arthroscope and the results compared to the findings of examination using a 2.5 mm 30° arthroscope system (standard). Three healthy horses were subsequently examined to determine the validity of the procedure in live animals in Phase II.ResultsNeedle and standard arthroscopy, in combination with mandibular manipulation, allowed evaluation of the caudal aspects of both joint compartments of the TMJ in Phase I. However, the extreme margins of the joint were more commonly visualized using standard arthroscopy. Live horses in phase II were restrained in stocks and both the rostral and caudal aspects of the DTJ and DMJ compartments of both TMJs were examined successfully understanding sedation and local analgesia. The use of a modified Guenther speculum allowed the mandible to be manipulated and offset, which facilitated a complete examination of the joint compartments. Despite adverse behavior encountered during the procedure in one horse, no surgical complications ensued.Main LimitationsNot blinded—bias; learning curve.ConclusionsThe needle arthroscope system is a relatively inexpensive diagnostic tool, which can be used to evaluate the TMJ in the absence of advanced diagnostic imaging such as computed tomography or magnetic resonance imaging. However, if arthroscopic treatment is required after advanced imaging and pre-operative diagnosis, superior image quality and ease of manipulation may favor the use of the standard equipment.

Arthroscopic debridement for infection after fracture fixation (IAFF) of the ankle: A case report.

Introduction and importanceInfection after fracture fixation (IAFF) is one of the most challenging issues for the lower-middle class socioeconomic. It is also related to unsatisfactory outcome of the treatment. Arthroscopy usually used to treat joint disease, but the evidence of arthroscopic management in IAFF is still limited.Case presentationWe present a case of 54-year-old female with IAFF of the ankle. An arthroscopic debridement and soft tissue release procedure were performed in this patient in one stage because the irrigation and debridement were sufficient. It showed a good result good functional outcome.Clinical discussionThe aims of IAFF treatment are to eradicate the infection, promote healing of soft tissue, prevent osteomyelitis, restore the joint function, and fracture consolidation. Arthroscopy in IAFF has been found to be safe and effective. In this case, arthroscopy was done in one stage because the debridement and irrigation were sufficient while the delay of the release would result in further pain and morbidity for the patient.ConclusionArthroscopic debridement with simultaneous release of impingement and stiffness is a novel, safe, and promising option in to eliminate both IAFF and its further complications of the ankle region.

Molecular hydrogen as a possible therapeutic factor in complex rehabilitation therapy in patients with muscular skeletal disorders (literature review)

The paper presents an analysis of foreign scientific and medical data on the therapeutic factor — molecular hydrogen. The effectiveness of its application in the complex therapy of many diseases is revealed. The effect is achieved due to the small size of the mo­lecule, which passes through biological membranes and inhibits dangerous free radicals in the mitochondria, as well as in the nuc­leus, which reduces the possibility of DNA damaging. Molecular hydrogen neutralizes oxidants in the brain due to its ability to cross the blood-brain barrier. It normalizes the functions and metabolic processes in the body and, as an antioxidant, is selective: it does not affect the useful free radicals involved in important metabo­lic processes and selectively eliminates only the most dangerous oxidants — hydroxyl radicals. Interacting with them, hydrogen converts them into water molecules without the formation of by-products and chain reactions. Unlike other known antioxidants, molecular hydrogen does not disrupt normal metabolism, does not cause negative changes in cells, activates the body’s own anti­oxidant systems. The possibility and expediency of the use of molecular hydrogen in the case of pathology of the musculoskeletal system has been confirmed. The peculiarities of its effect on bone and cartilage tissue in the experiment are shown. It has been determined that the use of molecular hydrogen is a new pharmacological strategy aimed at the selective removal of ONOO—, and can be an effective method in the treatment of joint diseases. Because cartilage receives nutrients through a diffusion-loading mechanism, and molecular hydrogen penetrates rapidly into tissues, it can be useful for the prevention of diseases of joints of degenerative origin. It reduces oxidative stress and slows down the reduction of matrix proteins and inhibition of proteinase degradation. Its effectiveness has been proven after injuries to the spinal cord, muscles and tendons, comorbid diseases such as hypertension, coronary heart disease, diabetes and metabolic syndrome. Key words. Molecular hydrogen, hydrogen water, hydrogen inhalations, joint diseases, consequences of musculoskeletal injuries, comorbid pathology.

Phytochemical Analysis and Anti-Inflammatory and Anti-Osteoarthritic Bioactive Potential of Verbascum thapsus L. (Scrophulariaceae) Leaf Extract Evaluated in Two In Vitro Models of Inflammation and Osteoarthritis.

Osteoarthritis (OA) is a complex disease, source of pain and disability that affects millions of people worldwide. OA etiology is complex, multifactorial and joint-specific, with genetic, biological and biomechanical components. Recently, several studies have suggested a potential adjuvant role for natural extracts on OA progression, in terms of moderating chondrocyte inflammation and following cartilage injury, thus resulting in an overall improvement of joint pain. In this study, we first analyzed the phenylethanoid glycosides profile and the total amount of polyphenols present in a leaf aqueous extract of Verbascum thapsus L. We then investigated the anti-inflammatory and anti-osteoarthritic bioactive potential of the extract in murine monocyte/macrophage-like cells (RAW 264.7) and in human chondrocyte cells (HC), by gene expression analysis of specifics inflammatory cytokines, pro-inflammatory enzymes and metalloproteases. Six phenylethanoid glycosides were identified and the total phenolic content was 124.0 ± 0.7 mg gallic acid equivalent (GAE)/g of extract. The biological investigation showed that the extract is able to significantly decrease most of the cellular inflammatory markers, compared to both control cells and cells treated with Harpagophytum procumbens (Burch.) DC. ex Meisn, used as a positive control. Verbascum thapsus leaf aqueous extract has the potential to moderate the inflammatory response, representing an innovative possible approach for the inflammatory joint disease treatment.

Fast &lt;i&gt;in vivo&lt;/i&gt; fixation of double network hydrogel to bone by monetite surface hybridization

Double network (DN) hydrogels, possessing biocompatibility, low sliding friction, high strength and toughness, are promising as artificial cartilages for next-generation joint disease treatment. For such application, a fast and robust fixation of DN hydrogel to bone tissue in vivo is indispensable. However, bonding the DN hydrogel that contains ∼90 wt % of water to bone is a grand challenge since glues do not work on hydrated surfaces. Recently, we reported that a DN hydrogel of its subsurface hybridized with low crystalline hydroxyapatite (HAp) can achieve robust fixation to bone after 4 weeks implantation in rabbit knees, owing to the HAp-induced osteogenesis penetration into the hydrogel matrix. For clinical application, achieving a quick fixation at the early stage of implantation remains as a next subject. In this study, instead of HAp, we hybridized calcium monohydrogen phosphate (monetite), which is a HAp precursor calcium phosphate salt, in the subsurface of the DN hydrogel and we observed an increase in the pushout resistance of the DN hydrogel to bone after 1 week implantation, prior to the HAp-induced osteogenesis penetration. In physiological environment, the monetite hybridized in the subsurface of the DN gel spontaneously dissolved to calcium and phosphate ions and then re-crystallized to more stable HAp. We consider that the HAp formed in the boundary between the gel and the bone forms physical interlocking that significantly enhances the frictional resistance against the pushout force. The fast temporally pre-fixation to the bone by monetite surface hybridization makes one step closer to the clinical application of the DN gels as artificial cartilages.

Diacerein ameliorates testosterone-induced benign prostatic hyperplasia in rats: Effect on oxidative stress, inflammation and apoptosis

Benign prostatic hypertrophy (BPH) is a serious medical condition among elderly male population. BPH pathogenesis has been linked to inflammation, cellular proliferation, oxidative stress and apoptosis. Diacerein (DIA) is a FDA approved anthraquinone drug that is used to treat joint diseases such as osteoarthritis. DIA has been studied for its potent anti-inflammatory and antioxidant effects, yet its role in managing BPH has not been investigated. In this study, DIA administration for two weeks at 50 mg/kg in testosterone-induced BPH rats significantly reduced prostate weight and index. Moreover, prostatic biochemical and structural features in BPH rats were significantly improved upon DIA treatment. Mechanistically, DIA treatment associated prostatic anti-hyperplastic effects were linked to downregulation of Nrf-2/HO-1 axis, downregulation of inflammatory TNF-a, IL-1β, IL-6, downregulation of the cell proliferative marker PCNA and upregulation of caspase-3 levels. In addition, DIA treatment upregulated prostatic antioxidant GSH, the enzymatic SOD and CAT activities and reduced prostatic lipid peroxidation levels. Altogether, the present study provides evidence that DIA treatment might limit BPH progression via its potent anti-oxidant, anti-inflammatory, anti-proliferative and apoptosis inducing effects.

Diacerein ameliorates kidney injury induced by cisplatin in rats by activation of Nrf2/Ho-1 pathway and Bax down-regulation

Cisplatin is an antineoplastic medicine used for solid tumor treatment. The main side effect that limits its dose is nephrotoxicity. Diacerein has been used for the treatment of joint diseases like osteoarthritis. It also has exhibited analgesic effects and antipyretic activities in animal models so this study targets to indicate the diacerein effect on nephrotoxicity induced by cisplatin in rats. Rats were distributed into four groups: normal healthy control; diacerein, which received diacerein daily by gastric gavage (50 mg/kg/day); cisplatin, which received only one intraperitoneal injection of cisplatin (6 mg/kg) and cisplatin and diacerein, which received diacerein daily after the cisplatin injection till 7th and 12th days, respectively. Diacerein treatment decreased kidney function markers so the cisplatin effect was reversed. Also, diacerein increased the renal antioxidants and decreased oxidative stress. Diacerein up-regulated Ho-1 (heme oxygenase 1), Nrf2 (Nuclear factor erythroid 2–related factor 2) and endothelial nitric oxide synthase (eNOS) genes expression, while down-regulated Bcl-2-associated X protein (Bax) gene expression. Furthermore, the renal transforming growth factor beta-1 (TGF-β1) decreased by the diacerein effect. Consequently, diacerein has a curative effect against cisplatin due to its anti-inflammatory, antioxidant, and antiapoptotic properties.

Environmental aspects and economic evaluation of new green hydrolysis method for waste feather processing

In recent years, an increase in the chicken meat process industry has been growing quickly, which brings a large amount of difficult to process waste, chicken feathers. These billions of kilograms create a serious waste problem over the world, furthermore, poultry feather processing is not only particularly difficult but also relatively expensive. In fact, there is no technology suitable for processing such huge quantities of feathers to guarantee sustainable development of the chicken meat industry together with processing of waste feathers. This article introduces a newly designed and original, highly efficient and environmentally friendly method of physicochemical hydrolysis of waste feathers. The hydrolysis is carried out in the presence of a weak organic carboxylic acid; thus, the resulting hydrolysate does not contain any salts or ashes. Therefore, a resulting hydrolysate, which includes a mixture of amino acids, peptides, proteins, glycoproteins and free fatty acids, is suitable for a variety of applications; e.g. as a chondroprotective agent in the treatment of joint diseases, nutrients for crop plants or targeted biostimulants for agriculture. This paper is focused not only on the process scale-up but also on environmental aspects and economic evaluations to bring general view of the process.Graphic abstract

Potential of Exosomes for Diagnosis and Treatment of Joint Disease: Towards a Point-of-Care Therapy for Osteoarthritis of the Knee.

In the knee joint, articular cartilage injury can often lead to osteoarthritis of the knee (OAK). Currently, no point-of-care treatment can completely address OAK symptoms and regenerate articular cartilage to restore original functions. While various cell-based therapies are being developed to address OAK, exosomes containing various components derived from their cells of origin have attracted attention as a cell-free alternative. The potential for exosomes as a novel point-of-care treatment for OAK has been studied extensively, especially in the context of intra-articular treatments. Specific exosomal microRNAs have been identified as possibly effective in treating cartilage defects. Additionally, exosomes have been studied as biomarkers through their differences in body fluid composition between joint disease patients and healthy subjects. Exosomes themselves can be utilized as a drug delivery system through their manipulation and encapsulation of specific contents to be delivered to specific cells. Through the combination of exosomes with tissue engineering, novel sustained release drug delivery systems are being developed. On the other hand, many of the functions and activities of exosomes are unknown and challenges remain for clinical applications. In this review, the possibilities of intra-articular treatments utilizing exosomes and the challenges in using exosomes in therapy are discussed.

Внутрисуставное введение глюкокортикостероидов в практике ревматолога

Intra-articular and periarticular use of drugs for the purpose of relieving inflammation of the synovial membrane or periarthritis is an integral part of the complex pathogenetic treatment of joint diseases. Effectiveness of local therapy with glucocorticosteroids (GCS )there is no doubt and depends on a number of factors, which should include the correct assessment of indications and contraindications, the correct choice of the drug, its dosage and technique of manipulation. The use of this method can significantly reduce the time of industrial and/or household disability in this extensive category of patients.However, the doctor's ignorance or disregard of the mandatory conditions and requirements for performing this minor surgical manipulation can lead to undesirable, including severe, discrediting consequences of the valuable method. Postgraduate training of doctors helps to solve the problem of non-professional approach to the appointment and conduct of intra-articular and periarticular manipulations using GCS.

State-of-the-Art of Eggshell Waste in Materials Science: Recent Advances in Catalysis, Pharmaceutical Applications, and Mechanochemistry.

Eggshell waste is among the most abundant waste materials coming from food processing technologies. Despite the unique properties that both its components (eggshell, ES, and eggshell membrane, ESM) possess, it is very often discarded without further use. This review article aims to summarize the recent reports utilizing eggshell waste for very diverse purposes, stressing the need to use a mechanochemical approach to broaden its applications. The most studied field with regards to the potential use of eggshell waste is catalysis. Upon proper treatment, it can be used for turning waste oils into biodiesel and moreover, the catalytic effect of eggshell-based material in organic synthesis is also very beneficial. In inorganic chemistry, the eggshell membrane is very often used as a templating agent for nanoparticles production. Such composites are suitable for application in photocatalysis. These bionanocomposites are also capable of heavy metal ions reduction and can be also used for the ozonation process. The eggshell and its membrane are applicable in electrochemistry as well. Due to the high protein content and the presence of functional groups on the surface, ESM can be easily converted to a high-performance electrode material. Finally, both ES and ESM are suitable for medical applications, as the former can be used as an inexpensive Ca2+ source for the development of medications, particles for drug delivery, organic matrix/mineral nanocomposites as potential tissue scaffolds, food supplements and the latter for the treatment of joint diseases, in reparative medicine and vascular graft producing. For the majority of the above-mentioned applications, the pretreatment of the eggshell waste is necessary. Among other options, the mechanochemical pretreatment has found an inevitable place. Since the publication of the last review paper devoted to the mechanochemical treatment of eggshell waste, a few new works have appeared, which are reviewed here to underline the sustainable character of the proposed methodology. The mechanochemical treatment of eggshell is capable of producing the nanoscale material which can be further used for bioceramics synthesis, dehalogenation processes, wastewater treatment, preparation of hydrophobic filters, lithium-ion batteries, dental materials, and in the building industry as cement.

Relevant Local Fatwā on The Issues of Using Human Tissues in Articular Cartilage Tissue Engineering Experimentation

In articular cartilage tissue engineering (ACTE) experimentation, the researchers have utilised cells and tissues sampled from the human donor for research purposes. The cells and tissues may be harvested from the living donor’s discarded tissues through a medical procedure, e.g. the total knee replacement surgery. The small pieces of a tissue sample taken from the human donor are essential to study the articular cartilage regeneration for treating joint disease, i.e. osteoarthritis. However, the procedure has raised some ethical and fiqh (Islamic jurisprudence) concerns. The study was done by utilising the secondary analysis of local Muslim jurists’ opinions (fatwā) related to the sampling of human biological samples. This paper explores the scenarios of using cell sources taken from the living human donor through the existing fatwā of local Muslim jurists (fuqahā`). The scenarios include: (1) taking samples from the living donor, and (2) discarding human tissue, as practised in ACTE experimentation. The current fatwā has shown that honouring every part of a human body is considered essential in Islam. ACTE researchers may utilise the biological samples from living donors as alternatives in studying articular cartilage regeneration. The donation of human biological samples for research purposes in ACTE experimentation, obtained from a medical procedure, may be permissible, should the stipulated terms and conditions were observed, and the procedure does not cause any additional harm to the donor.

CLINICAL AND LABORATORY EXAMINATION OF SYNOVIAL FLUID IN THE JOINT DISEASES DIAGNOSTICS. PART 1

Synovial fluid examination plays the important role in diagnosis and treatment of joint diseases. Nowadays it presents a substantial challenge in clinical and lab settings because of lack of manuals for the examination and processing of synovial fluid. It needed cross-disciplinary approach between orthopedic and trauma surgeons, rheumatologists, general practitioners, and clinical lab specialists to achieve the diagnostic and treatment spot-on goals in time. It is become incredibly important to develop the standard procedures for assessment of synovial fluid and its implementation into the practice for clinical diagnostic laboratories.

Development of image-guided puncture robot used in trigeminal neuralgia treatment

Recently, invasive surgery had developed rapidly and was widely used in the fields of tumour treatment, spine and joint disease treatment. At present, most of the analgesic puncture operations were...

Chitosan-based hydrogel for treatment of temporomandibular joint arthritis

Abstract To produce polysaccharide-based hydrogels and cerium (Ce3+) doped hydroxyapatite plus chitosan and collagen to enable future applications in the treatment of joint degeneration. Hydrogel production and characterization were performed with Fourier transform infrared spectroscopy (FTIR), thermogravimetry analysis (TGA) and cytotoxicity testing with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. A final biomaterial composition was Kelcogel® Gelana (58%), chitosan (22.3%), Ce3+ doped hydroxyapatite (10.7%) and bovine collagen (9%), or selected aspect material gelatinous physical color with whitish color and can be injected. The biomaterial composition was proven in the FTIR and TGA, which also provided the maximum supported temperature. In the MTT assay, despite the reduction in viability of the experimental group compared to the control group, cell viability remained approximately 90%. In the FTIR and TGA tests, the material composition was proven. The material does not present cytotoxic behavior for the MTT test, being an alternative for the treatment of joint diseases.