Abstract
Osteoarthritis (OA) is a complex disease, source of pain and disability that affects millions of people worldwide. OA etiology is complex, multifactorial and joint-specific, with genetic, biological and biomechanical components. Recently, several studies have suggested a potential adjuvant role for natural extracts on OA progression, in terms of moderating chondrocyte inflammation and following cartilage injury, thus resulting in an overall improvement of joint pain. In this study, we first analyzed the phenylethanoid glycosides profile and the total amount of polyphenols present in a leaf aqueous extract of Verbascum thapsus L. We then investigated the anti-inflammatory and anti-osteoarthritic bioactive potential of the extract in murine monocyte/macrophage-like cells (RAW 264.7) and in human chondrocyte cells (HC), by gene expression analysis of specifics inflammatory cytokines, pro-inflammatory enzymes and metalloproteases. Six phenylethanoid glycosides were identified and the total phenolic content was 124.0 ± 0.7 mg gallic acid equivalent (GAE)/g of extract. The biological investigation showed that the extract is able to significantly decrease most of the cellular inflammatory markers, compared to both control cells and cells treated with Harpagophytum procumbens (Burch.) DC. ex Meisn, used as a positive control. Verbascum thapsus leaf aqueous extract has the potential to moderate the inflammatory response, representing an innovative possible approach for the inflammatory joint disease treatment.
Highlights
Osteoarthritis (OA), known as degenerative arthritis, is the most common chronic condition of joint disease that affects up to 15% of the adult population
(52.3 mg/mL), peak 2 was identified as echinacoside (42.1 mg/mL), peak 3 was identified as forsythoside B (108.1 mg/mL), peak 4 was identified as verbascoside (254.8 mg/mL), peak 5 was identified as iso-verbascoside (142.9 mg/mL) and peak 6 was identified as martynoside (118.4 mg/mL) (Table 1)
The total phenylethanoid glycosides (PhEGs) content determined by high-pressure liquid chromatography (HPLC) was
Summary
Osteoarthritis (OA), known as degenerative arthritis, is the most common chronic condition of joint disease that affects up to 15% of the adult population. It is a disorder caused in part by damage of cartilage component and function, and deregulation of proinflammatory and anti-inflammatory pathways [1,2,3]. (V. thapsus), commonly known as common mullein, is a Eurasian plant belonging to the Scrophulariaceae family. It is a monocarpic and biennial herb that largely grows in cliffs, meadows, fields and ridges on dry, sandy and rocky soils
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call