Traditional Dressings Research Articles

Negative-pressure dressings in lower limb skin grafts: a randomised control trial of PICO versus standard dressings

Objective: Negative-pressure wound therapy (NPWT) can reduce the incidence of exudate and haematoma beneath a split-thickness skin graft (SSG) compared with traditional standard dressings. However, NPWT has not been universally adopted for lower limb SSGs despite evidence that its use is linked with improved graft take and a tendency towards early mobilisation. PICO (Smith and Nephew®), an ultra-lightweight NPWT device now available in Australia, can provide a smaller, more manageable NPWT dressing. The primary objective of this study was to compare lower limb graft take rate in PICO versus standard dressing groups, and a secondary objective was comparing quality of life (QOL) in these groups. Method: A prospective, randomised control trial was conducted of 71 lower limb wounds from 59 patients, with 36 wounds randomised to standard dressings and 35 to PICO dressings. Graft take was measured and expressed as a percentage of total wound area. Postoperative mobilisation day, patient comfort, complication frequencies, ease of dressing removal and QOL scores were also recorded. Results: The graft area percentage was not statistically significantly different between the PICO and standard dressing groups (p=0.054). All patients in the PICO group were mobilised by postoperative day one, but 8 per cent in the standard dressing group were still not mobilised by postoperative day five (PICO vs standard, p=0.003). There was no statistically significant difference in patient comfort, patient QOL or complication frequency in the PICO versus standard dressing group at any postoperative visit, but the PICO dressings were statistically significantly easier to remove (p=0.04). Conclusion: PICO dressings are not inferior or superior to standard dressings for lower limb SSGs.

Hydrogel Preparation Methods and Biomaterials for Wound Dressing.

Wounds have become one of the causes of death worldwide. The metabolic disorder of the wound microenvironment can lead to a series of serious symptoms, especially chronic wounds that bring great pain to patients, and there is currently no effective and widely used wound dressing. Therefore, it is important to develop new multifunctional wound dressings. Hydrogel is an ideal dressing candidate because of its 3D structure, good permeability, excellent biocompatibility, and ability to provide a moist environment for wound repair, which overcomes the shortcomings of traditional dressings. This article first briefly introduces the skin wound healing process, then the preparation methods of hydrogel dressings and the characteristics of hydrogel wound dressings made of natural biomaterials and synthetic materials are introduced. Finally, the development prospects and challenges of hydrogel wound dressings are discussed.

Modern Wound Dressings: Hydrogel Dressings.

Chronic wounds do not progress through the wound healing process in a timely manner and are considered a burden for healthcare system; they are also the most common reason for decrease in patient quality of life. Traditional wound dressings e.g., bandages and gauzes, although highly absorbent and effective for dry to mild, exudating wounds, require regular application, which therefore can cause pain upon dressing change. In addition, they have poor adhesional properties and cannot provide enough drainage for the wound. In this regard, the normalization of the healing process in chronic wounds is an extremely urgent task of public health and requires the creation and implementation of affordable dressings for patients with chronic wounds. Modern wound dressings (WDs) are aimed to solve these issues. At the same time, hydrogels, unlike other types of modern WDs (foam, films, hydrocolloids), have positive degradation properties that makes them the perfect choice in applications where a targeted delivery of bioactive substances to the wound is required. This mini review is focused on different types of traditional and modern WDs with an emphasis on hydrogels. Advantages and disadvantages of traditional and modern WDs as well as their applicability to different chronic wounds are elucidated. Furthermore, an effectiveness comparison between hydrogel WDs and the some of the frequently used biotechnologies in the field of regenerative medicine (adipose-derived mesenchymal stem cells (ADMSCs), mesenchymal stem cells, conditioned media, platelet-rich plasma (PRP)) is provided.

The Ideal Donor Site Dressing: A Comparison of a Chitosan-Based Gelling Dressing to Traditional Dressings.

Donor site wound management is critical in split-thickness skin graft surgeries. These sites typically recover in 7 to 14 days due to the dermal-imbedded keratinocytes that promote skin regeneration. An ideal donor site dressing can help to mitigate pain, reduce infection risk, promote hemostasis, and accelerate healing times. Additionally, this dressing would be easy to apply in the operating room, easily managed, and cost-effective. Chitosan-based gelling dressings (CBGD) possess many of these qualities that make an ideal donor site dressing. We conducted a retrospective chart review of patients who received CBGD as part of their postoperative wound care plan. We collected data on infections, hemostasis, dressing failure, and hospital course over a 14-month period where CBGD was used as the donor site dressing. One hundred and fourteen patients were evaluated. We found an infection rate of 7%, a bleed-through rate of 1.8%, and a re-application rate of 9.6%. The average CBGD cost per patient was $75.15. CBGD has acceptable infection rates, and pain scores as traditional donor site dressings. However, it possesses several qualities of a suitable donor site dressing notably swift healing rates, impressive hemostatic property, and low cost. Our study supports the idea that CBGD is a suitable donor site dressing for split-thickness skin graft surgeries.

Injectable stretchable self-healing dual dynamic network hydrogel as adhesive anti-oxidant wound dressing for photothermal clearance of bacteria and promoting wound healing of MRSA infected motion wounds

Due to the frequent movement and stretching of skin, traditional wound dressings are difficult to adapt to motion wounds on the stretchable parts of the body surface (e.g. elbow, hip and knee). Moreover, chronic motion wounds are often accompanied by infections. To address these issues, a series of injectable adhesive self-healing photothermal dual dynamic Schiff base network hydrogels were developed based on adipic dihydrazide modified hyaluronic acid, benzaldehyde group functionalized poly(ethylene glycol)–co-poly(glycerol sebacate) and cuttlefish melanin nanoparticles, and their excellent tissue adhesion, stretchability and self-healing properties enable them to adapt to the frequent movement of motion wounds. Meanwhile, the hydrogels can prevent and treat motion wound infections through photothermal antibacterial therapy, and exhibit multifunctions (including anti-oxidation, hemostasis, exudate absorption and sustained release property) to promote wound healing. In in vivo normal and infected full-thickness skin defect motion wound models, the hydrogel dressings significantly prevented wound infections and promoted wound healing with milder inflammation, higher granulation tissue thickness and collagen disposition. Overall, the adhesive self-healing photothermal hydrogels can adapt to frequent movement of motion wounds, and can improve infection and promote healing in both normal and infected motion wounds, indicating their great potential in motion wound treatment in clinics.

Efficacy of Oxidized Regenerated Cellulose/Collagen Dressing for Management of Skin Wounds: A Systematic Review and Meta-Analysis.

Objective The purpose of this study was to evaluate the wound healing efficacy of oxidized regenerated cellulose (ORC)/collagen dressing and ORC/collagen/silver-ORC dressings compared to standard of care or control in treatment of chronic skin wounds such as diabetic foot ulcers (DFUs), venous leg ulcers (VLUs), and pressure injuries sore ulcers (PISUs). Methods An electronic search was carried out in four popular databases PubMed, Scopus, Embase, and CENTRAL to identify thirteen included studies, comparing the clinical efficacy of ORC/collagen dressings when compared to control in management of chronic skin wounds, especially DFUs, VLUs, and PISUs, and skin graft donor site wounds. Results Consolidated data from thirteen comparative clinical studies undertaken for management of DFUs, VLUs, and PISUs showed favorable outcomes towards use of ORC/collagen compared to other traditional and hydrocolloid foam dressings in terms of wound healing rate (P=0.02) and percentage wound relative reduction (P=0.003). The time taken to achieve complete wound healing in the included studies did not show any statistical significant difference (P=0.24). There was no significant difference in adverse events between ORC/collagen-treated group and comparative group (P=0.19). Conclusion ORC/collagen wound dressings are beneficial in terms of improved wound healing rate and percentage wound relative reduction compared to already existing traditional standard of care with non-MMP, inhibiting biomaterials such as moistened gauze, autologous growth factors, hydrocolloid foam dressings, or ovine extracellular matrix.

Study on scar repair and wound nursing of chitosan-based composite electrospun nanofibers in first aid of burn

Bacterial infection and oxidative stress are serious complications in emergency burn patients, as the increase of oxygen free radicals in burn wounds can aggravate vascular endothelial cell injury, make the wounds ischemic and hypoxic, and delay wound healing. Traditional dressings cannot meet the first-aid needs of burn patients. In this study, polycaprolactone/chitosan (PCL/CS) was used as an electrospun nanofiber matrix, and curcumin (CUR), a molecule with excellent anti-inflammatory and antioxidant properties, was introduced to construct polycaprolactone/ chitosan graft copolymer-zein-curcumin electrospun nanofibers (PCL/CS-ZE-CUR). The results of clinical experiments suggest that compared with traditional dressings, based on the excellent mechanical properties and antibacterial activity of PCL/CS, the new dressing can exhibit oxygen free radical-scavenging abilities of CUR to accelerate wound healing and is expected to provide a beneficial upgrade for wound emergency care.

Experimental Substantiation of Antimicrobial Efficiency of a New Composite Polymeric Material Based on Poly(2-Hydroxyethyl Methacrylate) under the Action of Low-Intensity Current without External Power Supplies

Active use of polymeric materials has become an integral part of all areas of modern medicine. Wound dressings capable of prolonged release of drugs directly into the lesion occupy a special place among them. The possibility of using such materials in the presence of low-intensity currents without external power supplies in a comprehensive treatment program for patients with burn injuries remains promising. The aim of the work is to study experimentally the antimicrobial efficacy of a new composite polymeric material based on poly(2-hydroxyethyl methacrylate), saturated with the antiseptic decamethoxine, under conditions of low-intensity current without external power supplies. The method of free radical thermal polymerization of a mixture of liquid monomer 2-hydroxyethyl methacrylate, crosslinking agent triethylene glycol dimethacrylate, polymerization initiator azobisisobutyronitrile was used for the synthesis of composite polymeric material. In addition, fourfold volume of distilled water as a pore-forming agent and decamethoxine as an antimicrobial component were administered. Known dressings of synthetic and biological origin were selected for comparison, some of which were pre-soaked in a 0.02% solution of decamethoxine. The study of conductivity of the materials without external power supplies was performed on the surface of a dense nutrient medium in a Petri dish using VITA-01M measuring device. Determination of antibacterial properties was performed by diffusion into agar. The obtained results allowed to establish the ability of the suggested polymeric material to conduct low-intensity currents without external power supplies, exceeding the duration of other traditional dressings. Comparison of antimicrobial activity of the studied samples confirmed the synergism of the action of physical factors and a new polymer-based composite material with the addition of antimicrobial substance to inhibit the growth of the test museum and clinical strains of Staphylococcus aureus. The ability of low-intensity currents without external power supplies to potentiate the antimicrobial properties of a new composite polymeric material based on poly(2-hydroxyethyl methacrylate), modified with a pore-forming agent, with the addition of decamethoxine was experimentally established.

Fluorescent Light Energy and Chronic Lesions: A Winning Association.

Background:Chronic ulcers represent a challenge for healthcare professionals and a large expense for national health care systems for their difficulty in achieving complete healing and for their high incidence of recurrence. With the progressive aging of the general population, the incidence of these injuries will only increase, further affecting the public health budget, hence the need to find new strategies for their management. The purpose of this study was to share the experience of the Complex Operational Unit of Plastic Surgery of the University Hospital of Padua with fluorescent light energy therapy, outlining its role in the treatment of chronic ulcers in the daily use outside the previous EUREKA study.Methods:In this case series study, we enrolled 15 patients with chronic ulcers of any etiology between January 2018 and July 2019 and we treated them using fluorescence light energy. We evaluated efficacy and safety endpoints reporting data in excel files completed by medical staff during the study.Results:The study confirms the effectiveness of fluorescent light energy inducing chronic ulcer healing, regardless of etiology, or at least preparing the lesions for a skin graft closure surgery. The system showed a low rate of complications established by patient adherence to treatment. Patients also reported a reduction in pain both at home and during outpatient dressings.Conclusion:Based on our experience, fluorescent light energy shows an excellent safety and efficacy profile in chronic ulcers no more responsive to traditional dressings and/or surgery.

Could intermittent change of conventional dressing affect risk of periprosthetic joint infection after primary total joint arthroplasty?

Periprosthetic joint infection (PJI) is one of the most dreaded and challenging complications after total joint arthroplasty (TJA). The aim of this study was to evaluate the effect of keeping the dressing without change on the occurrence of PJI in patients undergoing TJA. 4877 Patients with a minimum follow-up of 90days were included to investigate the effect of dressing on the PJI occurring within 3months of surgery. Patients were divided into two consecutive groups as the intermittent change of traditional dressing (group 1-before 2019) and keeping dressing for 5days without change (group 2-after 2019). A backward stepwise logistic regression model was used to estimate independent risk factors for PJI. Group 1 and group 2 consisted of 4172 and 705 patients, and the numbers of diagnosed PJI cases in the groups were 40 (1.0%) and 10 (1.4%), respectively (p = 0.1). The backward stepwise logistic regression model analysis revealed that keeping the dressing unchanged and removing it after the first week postoperatively was not an independent risk factor for the occurrence of PJI. Older age, diabetes mellitus and coronary artery diseases were independent risk factors for PJI (p < 0.05). Our study results present, that intermittent change of conventional dressing is unnecessary, because it does not decrease the risk of PJI after TJA. Level III Therapeutic.

Wound Dressings for Hip and Knee Total Joint Arthroplasty: A Narrative Review.

Dressing choice following lower-extremity total joint arthroplasty has substantial ramifications for postoperative outcomes and should be carefully made to prevent complications such as periprosthetic joint infection. Patient risk factors are essential components in the selection of wound dressings in total joint arthroplasty. Traditional dressings are inexpensive per unit; nevertheless, the associated higher complication profile in patients at a high risk for poor wound healing and sequelae-associated costs may outweigh the up-front savings. Modern dressings have the potential to yield better safety outcomes and increased patient satisfaction; however, there is a paucity of evidence regarding the ideal interactive dressing. Active dressings, such as silver-ion dressings and closed-incisional negative-pressure wound therapy, have shown promising results to reduce surgical site and periprosthetic joint infection, especially in patients at a high risk for poor wound healing following hip and knee total joint arthroplasty.

Preparation and Application of Quaternized Chitosan- and AgNPs-Base Synergistic Antibacterial Hydrogel for Burn Wound Healing.

Infection is the major reason that people die from burns; however, traditional medical dressings such as gauze cannot restrain bacterial growth and enhance the healing process. Herein, an organic- and inorganic-base hydrogel with antibacterial activities was designed and prepared to treat burn wounds. Oxidized dextran (ODex) and adipic dihydrazide grafted hyaluronic acid (HA-ADH) were prepared, mixed with quaternized chitosan (HACC) and silver nanoparticles to fabricate Ag@ODex/HA-ADH/HACC hydrogel. The hydrogel, composed of nature biomaterials, has a good cytocompatibility and biodegradability. Moreover, the hydrogel has an excellent antibacterial ability and presents fast healing for burn wounds compared with commercial Ag dressings. The Ag@ODex/HA-ADH/HACC hydrogel will be a promising wound dressing to repair burn wounds and will significantly decrease the possibility of bacterial infection.

Wound Healing: From Passive to Smart Dressings.

The universal increase in the number of patients with nonhealing skin wounds imposes a huge social and economic burden on the patients and healthcare systems. Although, the application of traditional wound dressings contributes to an effective wound healing outcome, yet, the complexity of the healing process remains a major health challenge. Recent advances in materials and fabrication technologies have led to the fabrication of dressings that provide proper conditions for effective wound healing. The 3D-printed wound dressings, biomolecule-loaded dressings, as well as smart and flexible bandages are among the recent alternatives that have been developed to accelerate wound healing. Additionally, the new generation of wound dressings contains a variety of microelectronic sensors for real-time monitoring of the wound environment and is able to apply required actions to support the healing progress. Moreover, advances in manufacturing flexible microelectronic sensors enable the development of the next generation of wound dressing substrates, known as electronic skin, for real-time monitoring of the whole physiochemical markers in the wound environment in a single platform. The current study reviews the importance of smart wound dressings as an emerging strategy for wound care management and highlights different types of smart dressings for promoting the wound healing process.

A Pilot Study of Negative Pressure Therapy with Autologous Skin Cell Suspensions in a Porcine Model

Negative pressure wound therapy (NPWT) is an option for securing meshed split thickness skin grafts (mSTSGs) after burn excision to optimize skin graft adherence. Recently, the use of autologous skin cell suspension (ASCS) has been approved for use in the treatment of burn injuries in conjunction with mSTSGs.To date, limited data exists regarding the impact of NPWT on healing outcomes when the cellular suspension is utilized. It was hypothesized that NPWT would not negatively impact wound healing of ASCS+mSTSG. A burn, excision, mSTSG, ASCS ± NPWT model was used. Two Duroc pigs were utilized in this experiment, each with 2 sets of paired burns. Four wounds received mSTSG+ASCS+NPWT through post-operative day 3, and 4 wounds received mSTSG+ACSC+ traditional ASCS dressings. Cellular viability was characterized prior to spraying. Percent re-epithelialization, graft-adherence, pigmentation, elasticity, and blood perfusion and blood vessel density were assessed at multiple time points through 2 weeks. All wounds healed within 14 days with minimal scar pathology and no significant differences in percent re-epithelialization between NPWT, and non-NPWT wounds were observed. Additionally, no differences were detected for pigmentation, perfusion, or blood vessel density. NPWT treated wounds had less graft loss and improved elasticity, with elasticity being statistically different. These data suggest the positive attributes of the cellular suspension delivered are retained following the application of negative pressure. Re-epithelialization, revascularization, and repigmentation are not adversely impacted. The use of NPWT may be considered as an option when using ASCS with mSTSGs for the treatment of full-thickness burns.

Impact of Previous Irradiation on Wound Healing after Negative Pressure Wound Therapy in Head and Neck Cancer Patients-A Systematic Review.

Simple SummaryThe study has focused on the use of Negative Pressure Wound Therapy (NPWT) specifically in head and neck cancer patients. The sole purpose of using NPWT is to expedite the process of healing as most of the head and neck cancer patients are bound to get post-operative radiotherapy and any delay would further lengthen the treatment and affect the outcome. Previous irradiation and Diabetes Mellitus have detrimental impact on wound healing after NPWT. (1) Background: Negative pressure wound therapy (NPWT) has been effectively used for wound management in comparison to traditional dressings. The purpose of this study was to provide an evidence-based review of NPWT in head and neck cancer patients, as well as the impact of previous irradiation and other risk factors on wound healing. (2) Material and Methods: We conducted a comprehensive search in PubMed, Medline, Embase, Web of Science, and Cochrane Library databases for relevant literature. (3) Results: 15 studies fulfilled the inclusion criteria. The most common etiologies requiring NPWT were defects post tumor resection and flap reconstruction and oro/pharyngo-cutaneous fistulas. The neck was found to be the most common site of involvement (47.3%). The overall wound healing response rate was 87.5%. The median negative pressure recorded was 125 mm of Hg, with a median dressing change time of three days. Previous irradiation (p = 0.01; OR = 4.07) and diabetes mellitus (DM) (p = 0.001; OR = 5.62) were found to be significantly associated with delayed wound healing after NPWT. (4) Conclusion: NPWT treats complex wounds in head and neck cancer patients and should represent a significant armamentarium in head and neck cancers. Previous irradiation and DM have detrimental effects on wound healing after NPWT.

A Nanoporous Graphene/Nitrocellulose Membrane Beneficial to Wound Healing.

Adequate treatment of skin wounds is vital to health. Nitrocellulose bandage as a traditional wound dressing is widely used for wound healing, but its limited air permeability and poor sterilization need to be improved for enhancing the actual efficacy. Here, nanoporous graphene (NPG) is used to mix into nitrocellulose for preparing a composite membrane, which exhibits a moderate transmission rate of water vapor, excellent toughness performance, and good biocompatibility. Moreover, the membrane shows an excellent broad-spectrum antibacterial property (>98%, Escherichia coli; >90%, Staphylococcus aureus) and can reduce the risk of microbial infection for the body after trauma. Importantly, after using the nanoporous graphene/nitrocellulose membrane, the wound closure percentage reaches 93.03 ± 1.08% at 7 days after the trauma, and the degree of skin tissue recovery is also improved significantly. Therefore, this study develops a highly efficient wound healing dressing, which is expected to be used directly in clinics.

Acellular fish skin enhances wound healing by promoting angiogenesis and collagen deposition

Acellular matrix is a type of promising biomaterial for wound healing promotion. Although acellular bovine and porcine tissues have proven effective, religious restrictions and risks of disease transmission remain barriers to their clinical use. Acellular fish skin (AFS), given its similarity to human skin structure and without the aforementioned disadvantages, is thus seen as an attractive alternative. This study aims to fabricate AFS from the skin of black carp (Mylopharyngodon piceus), evaluate its physical and mechanical properties and assess its impact on wound healing. The results showed that AFS has a highly porous structure, along with high levels of hydrophilicity, water-absorption property and permeability. Furthermore, physical characterization showed the high tensile strength of AFS in dry and wet states, and high stitch tear resistance, indicating great potential in clinical applications. Cell Counting Kit-8 was used to test the viability of L929 cells when culturing in the extracts of AFS. Compared with the control group, there is no significant difference in optical density value when culturing in the extracts of AFS at days 1, 3 and 7 (*p > 0.05). In vivo wound healing evaluation then highlighted its promotion of angiogenesis and collagen synthesis, its function in anti-inflammation and acceleration in wound healing. Therefore, this study suggests that AFS has potential as a promising alternative to mammal-derived or traditional wound dressing.

VAC Therapy versus Traditional Wound Dressing: Comparative Effects of Nursing Training Program on Patients' Outcomes

Abstract: Background: Nurses should have adequate training about appropriate dressing selection to enhance the quality of life for patients with chronic wound. Aim; To assess the effects of Nursing Program about Vacuum-Assisted Closure Therapy versus Traditional Wound Dressing, on Patients' Outcomes. Method: Design; Quasi-experimental (Preposttest), a comparative research design between both groups. Setting: The trauma departments and outpatients' clinic at Assiut University Hospital. Subjects: Total coverage of nurses (N.= 40) and a randomized 60 patients with (chronic wound), both groups nurses and patients were divided into two equal groups. Tools: (I) Nurses' interview questionnaire (demographic characteristics, knowledge, and observation checklist), (II): Patients' assessment (personnel and clinical data) and (III): Patients' outcomes sheet; Bates-jensen wound assessment scale and WoundQuality of life scale. Results: A statistically significant difference between nurses' knowledge and practice pre and post-test. Also, between the traditional wound dressing and VAC therapy groups regarding their outcomes (wound healing and wound quality of life) with a positive correlation between them. Conclusion: The nursing program had a positive effect on nurses` knowledge, practice and on patients' outcomes who managed with VAC therapy were better than the other group who managed with the traditional wound dressing. Recommendations: Continuous instructions and in-service training programs in the trauma departments to improve nurses' knowledge and practice. Apply VAC therapy in managing chronic wound for a better healing and high quality of life.

Investigation into the physical properties, antioxidant and antibacterial activity of Bletilla striata polysaccharide/chitosan membranes

Traditional wound dressings and formulations, such as cream, gauze, cotton wool and gel, are disadvantaged by short residence time, poor leakage and air permeability, poor patient compliance, and the minimal preservation in wet environment. This study is purposed to develop new biodegradable, antioxidant, and antimicrobial membranes based on two natural polysaccharides, Bletilla striata polysaccharide (BSP) and chitosan (CS). The developed films were characterized by SEM, FTIR spectroscopy, NMR spectroscopy and X-ray diffraction to examine surface morphology and internal structure, while TG analysis was conducted to explore the thermal properties of the films. The physical properties of the films were also improved significantly after the introduction of BSP. The biological activity of developed films was assessed by means of antioxidant and antibacterial assay for the further research as a potential wound dressing. The CCK-8 assay revealed that the developed films showed a significant improvement of cell viability, biocompatibility and non-toxicity. These researches demonstrated that BSP/CS films can be applied as suitable materials for the development of biomaterial matrix in novel wound dressing.

138 Evaluation of Healing Outcomes Combining Negative Pressure Wound Therapy with Autologous Skin Cell Suspension and Meshed Autografts: Pre-Clinical and Clinical Evidence

Abstract Introduction Negative pressure wound therapy (NPWT) is an option for securing meshed split thickness skin grafts (mSTSGs) after burn excision to optimize skin graft adherence, working by minimizing disruption by shear forces and promoting the continual removal of wound bed drainage. Recently, the use of autologous skin cell suspension (ASCS) has been approved for use in treating full-thickness burn injuries in conjunction with mSTSGs. Limited data exists regarding the impact of NPWT on healing outcomes when the cellular suspension is utilized. It was hypothesized that NPWT in conjunction with ASCS+mSTSGs would aid in skin graft adherence without compromise to healing outcomes. Methods In this study, a Duroc pig model of burn, excision, mSTSG, ASCS + NPWT was used (n=2), where each animal had 2 sets of paired burns. Four wounds received mSTSG+ASCS+NPWT through post-operative day 3, and 4 wounds received mSTSG+ACSC+ traditional ASCS dressings. Percent re-epithelialization was measured using digital planimetry and Image J. Graft-adherence was evaluated using a scale with blinded reviewers (0=no graft loss, 4= &amp;gt;50% graft loss). Histological architecture, pigmentation, elasticity, and blood perfusion and blood vessel density were assessed at multiple time points through 2 weeks. After the evaluation of its effectiveness in animal models, the same surgical technique, including NPWT, was used in patients with full-thickness burns (n=9), and wound healing trajectories were described. Results In the Duroc pig study, all wounds healed within 14 days with minimal scar pathology and no significant differences in percent re-epithelialization between NPWT and non-NPWT wounds were observed (61.09 ± 9.01 and 61.15 ± 0.82% at Day 7). Additionally, no differences were detected for pigmentation, perfusion, or blood vessel density. Overall, the non-NPWT group had higher amounts of graft loss (1.0 ± 1.41 vs. 0 ± 0). NPWT-treated wounds had significantly improved elasticity (NPWT=109.5 ± 21.23 vs. non-NPWT=177.5 ± 35.4, p&amp;lt; 0.05). There were no differences in histological architecture between treatment groups. Patients had a median age of 53 (37–69), and median TBSA of 12.5 (8–18) resulting primarily from scald burns (67%). There were no reported morbidities, and all wounds were re-epithelialized within an expected time period. The use of NPWT promoted graft adherence, and was useful as a bolster dressing in wounds that crossed joints. Conclusions These data suggest the positive attributes of the cellular suspension delivered are retained following the application of NPWT. Re-epithelialization, revascularization, and repigmentation are not adversely impacted.