Tested Research Articles

Testicular hormones mediate robust sex differences in impulsive choice in rats.

Impairments in choosing optimally between immediate and delayed rewards are associated with numerous psychiatric disorders. Such 'intertemporal' choice is influenced by genetic and experiential factors; however, the contributions of biological sex are understudied and data to date are largely inconclusive. Rats were used to determine how sex and gonadal hormones influence choices between small, immediate and large, delayed rewards. Females showed markedly greater preference than males for small, immediate over large, delayed rewards (greater impulsive choice). This difference was neither due to differences in food motivation or reward magnitude perception, nor was it affected by estrous cycle. Ovariectomies did not affect choice in females, whereas orchiectomies increased impulsive choice in males. These data show that male rats exhibit less impulsive choice than females and that this difference is at least partly maintained by testicular hormones. These differences in impulsive choice could be linked to gender differences across multiple neuropsychiatric conditions.

An Evaluation of Abdominal Manifestations by Ultrasonography in Immunodeficiency Patients

Introduction: The major target of HIV is the immune system and the abdominal pathologies are second most commonly found in patients of AIDS after pulmonary manifestations. These abdominal manifestations are on increase may be due to immunocompromised status of these patients and due to antiretroviral therapy which has increased life expectancy in these patients. Purpose of this study is to describe abdominal findings and to evaluate clinical utility of USG in HIV patients with the use of abdominal probe, high frequency probe and Color Doppler USG. Methods: This Retrospective Analytical study involved 60 randomly selected HIV patients attending to Local ART Units . Prior Consent was obtained from Local ART Centre to see the records of the patients from there MRD .Patients of all age groups and both sex having cataract were included in the study Results: This study includes 60 HIV patients, 37 patients were male and 23 patients were female. Age group studied was 20 yrs to 65 yrs referred from ART Department . Most prevalent age group was from 40 - 49 yrs that was 23 patients, next was 30 - 39 yrs i.e. 20 patients, third in declining order was 20 - 29 yrs i.e. 12 patients and less prevalent age groups were from 50 - 59 yrs and above 60 - 70 yrs. These patients had diverse abdominal findings. Out of 60 patients 3 had no abdominal findings. Hepatomegaly was found in 33 patients, fatty hepatic changes in 14 patients and in one case there was Hepatic abscess. Lymphadenopathy was found in 28 cases. Splenomegaly was found in 20 cases and in 6 cases there were also having splenic abscesses. Ascites was found in 12 cases it was septated and non septated ascites. Cholecystitis in 4 cases. Bowel wall thickening in 5 cases in most of cases it was thickening of ileum and Ileocaecal junction thickening. Other findings were renal parenchymal disease in 2 cases, Bulky pancreas in 2 cases, cervicitis in 2 cases, one case each of cystitis and pyocele. Testicular abscess in 2 cases and gluteal abscess in one case along with other abdominal findings. Many patients had two or more pathologies. Conclusion: AIDS is multisystem disease but its demographic and clinical pattern remains same globally. USG is optimally suited for its clinical management especially in developing countries like India. Results of sonographically evaluated cases of abdominal findings are comparable to other diagnostic modalities like CT scan. The ease of availability, diagnostic accuracy of USG and its affordable cost and interventional and follow up advantages makes USG first choice of investigation for evaluation of abdominal findings. Clinico-pathological correlations can further increase its diagnostic accuracy.

A Dynamic-Key Based Secure Scan Architecture for Manufacturing and In-Field IC Testing

The design for testability (DFT) technology based on scan chains is widely used in industry to increase the testability of circuits. However, it also leads to a potential security problem that attackers can use scan chains as a backdoor to attack a system. Common methods to defend such attacks include disabling the scan chain after manufacturing test or employing some secret keys to encrypt/decrypt scan data or to verify the identities of users. The former would make in-field testing impossible and the latter would require storing keys in memory which might also undergo high risk of memory attacks. In this paper we propose a dynamic-key based secure scan architecture that works together with an intrinsic Physical Unclonable Function (PUF) of chips to defend both scan-based and memory attacks while facilitating both manufacturing and in-field testing. A system equipped with this secure architecture will shift out true circuit responses only when legal test patterns are shifted into the scan chains. Moreover, no test key will be stored in memory, hence no memory attacks are possible. We also leverage the PUF to distinct the legal test patterns for different manufactured chips so as to further protect chips. Analysis results show that our protection scheme can achieve a very high security level without sacrificing system performance, testability and diagnosability.

Effect of dietary supplementation of omega-3 rich oils on seminal characteristics, oxidative stress and plasma testosterone levels in Beetal bucks

The objective of the present study was to evaluate the efficacy of dietary supplementation of omega-3 rich oils, chia seed oil (CSO; Salvia hispanica) and linseed oil (LSO; Linum usitatissimum), on seminal characteristics, and plasma testosterone levels in Beetal bucks. The bucks were fed diet supplemented with either LSO or CSO @1% on dry matter basis or un- supplemented diet for 100 days. Daily intake of omega-3 fatty acids was 0.60 (control diet), 4.04 (diet supplemented with LSO) and 4.57 g (diet supplemented with CSO). Ultrasonography revealed no significant improvement in the testicular size yet the growth rate was much high in animals fed omega-3 supplemented diet. Ejaculate volume, individual motility, viability, sperm concentration, plasma membrane and acrosome integrity were significantly higher in bucks fed CSO supplemented diet as compared to LSO and control diet. The percentage of abnormal spermatozoa was significantly less in bucks fed CSO and LSO diet than control. Melanoaldehyde (MDA, μmole/109 sperm) concentration was lowest in bucks fed CSO diet followed by LSO and control diet. Activity of superoxide dismutase, glutathione reductase and catalase was significantly higher in spermatozoa of bucks fed CSO and LSO diet as compared to control. Bucks fed CSO and LSO supplemented diet had nonsignificantly higher plasma testosterone concentrations than control. These findings indicated that CSO supplementation in the diets may be helpful in improving the process of spermatogenesis, sperm output, quality and fertility of bucks.

Foundation size effect on the efficiency of seismic base isolation using a layer of stone pebbles

The effect of the foundation size on the efficiency of seismic base isolation using a layer of stone pebbles is experimentally investigated. Four scaled models of buildings with different stiffnesses (from very stiff to soft) were tested, each with the so-called small and large foundation, and exposed to four different accelerograms (different predominant periods and durations). Tests were conducted so that the strains in the model remained elastic and afterwards the models were tested until collapse. Each model was tested for the case of the foundation being supported on a rigid base and on an aseismic layer. Compared to the smaller foundation, the larger foundation results in a reduced rocking effect, higher earthquake forces and lower bearing capacity of the tested models, with respectable efficiency (reduced strain/stress, displacement and increase of the ultimate bearing capacity of the model) for the considered seismic base isolation compared to the foundation on a rigid base.

Características de carcaça, composição tecidual e análise sensorial da carne de bezerros aleitados com níveis crescentes de soro de queijo em pó

Objetivou-se avaliar a influência do aleitamento com soro de queijo em pó associado ao leite em pó refugado de indústria sobre as características, rendimentos e qualidade da carne de bezerros de origem leiteira. Foram utilizados 40 bezerros machos distribuídos em quatro tratamentos e dez repetições: LI - leite de integral (controle); LP - 100% leite em pó; LPS1 – 80% leite em pó + 20% soro de queijo em pó e LPS2 - 60% de leite em pó + 40% de soro de queijo. Na carcaça, foram avaliados os valores de pH e temperatura ao abate e após 24 horas de resfriamento. Os parâmetros físico-químicos, atividade de água, acidez, umidade, cinzas, proteínas e lipídeos da carne foram avaliados no músculo Longissimus dorsi. O pH médio da carcaça pós-abate foi de 6,6 e o pH após resfriamento de 5,3. Os valores médios de umidade, proteína, extrato etéreo e cinzas foram: 73,36%; 23,03%; 2,00% e 1,06%, respectivamente. Na análise sensorial foram avaliados cor, maciez, sabor, suculência, impressão global e intenção de compra, não apresentou diferença significativa. As dietas testadas não interferiram nas características de carcaça, composição tecidual e nos atributos qualitativos da carne dos animais avaliados, sendo possível a substituição do leite integral por dietas a base de soro de queijo em pó e leite em pó, não apresentando prejuízos ao desenvolvimento de bezerros leiteiros em fase de aleitamento.

Selenium sources differ in their potential to alleviate the cadmium-induced testicular dysfunction

Cadmium (Cd), a major environmental contaminant, is closely associated with male reproductive health. Selenium (Se) has been recognized as an effective chemo-protectant against Cd toxicity, but the underlying mechanisms remain unclear. The objective of present study was to illustrate the toxic effect of Cd on testis, and then compare the antagonistic effect among different Se sources on growth performance, testicular damage, ion homeostasis, antioxidative potential, and the expression of selenotranscriptome and biosynthetic related factors in Cd-treated chicken. Male chickens were fed with (Ⅰ) Control group: basal diet; (Ⅱ) Cd group: basal diet with 140 mg/kg CdCl2; (Ⅲ) YSe + Cd group: basal diet with 140 mg/kg CdCl2 and 3 mg/kg Yeast-Se; (Ⅳ) NSe + Cd group: basal diet with 140 mg/kg CdCl2 and 1 mg/kg Nano-Se; (Ⅴ) SSe + Cd group: basal diet with 140 mg/kg CdCl2 and 3 mg/kg Na2SeO3. It was observed that different Se treatments dramatically alleviated Cd-induced testicular developmental disorder, ion homeostasis disorder, hormone secretion disorder and oxidative stress. Simultaneously, Se mitigated Cd-induced testicular toxicity by regulating selenoprotein biosynthetic related factors to promote selenoprotein transcription. Finally, this study indicated that dietary supplementation of Yeast-Se produced an acceptable Se form to protect testis from Cd exposure.

Pregabalin administration and withdrawal affect testicular structure and functions in rats.

The aim of this prospective experimental study was to investigate the effects of pregabalin (PG) administration and withdrawal on testicular structures and functions in rats. A total of 24 male Wistar rats were divided into two groups (n=12 each): a control group received normal saline, and PG-treated group received 62mgkg-1 day-1 PG for 2months. Half the animals of each group were sacrificed for the collection of blood and testicular samples. The remaining animals were bred for another 2months without treatment before collection of blood and testicular samples. PG administration decreased testosterone and increased luteinising hormone (LH) and follicle-stimulating hormone (FSH) levels versus controls. PG withdrawal led to a decrease in both FSH and LH and an increase in testosterone levels versus saline withdrawal. Compared to controls, PG administration caused degeneration of seminiferous tubules and decreased the number of spermatogenic but increased the number of Leydig cells. After PG withdrawal, these cells showed a rebound reverse. Reduced glutathione levels increased with PG administration while PG withdrawal increased malondialdehyde levels. Conclusion: PG administration affected testicular morphometry, gonadotrophic and sex hormones; however, there was a rebound reversal in all these parameters and a significant oxidative stress in PG withdrawal.

Multiscale analysis of SRY-positive 46,XX testicular disorder of sex development: Presentation of nine cases.

46,XX testicular disorder of sex development (46,XX TDSD) is a relatively rare condition characterised by the presence of testicular tissue with 46,XX karyotype. The present study aims to reveal the phenotype to genotype correlation in a series of sex-determining region Y (SRY)-positive 46,XX TDSD cases. We present the clinical findings, hormone profiles and genetic test results of six patients with SRY-positive 46,XX TDSD and give the details and follow-up findings of our three of previously published patients. All patients presented common characteristics such as azoospermia, hypergonadotropic hypogonadism and an SRY gene translocated on the terminal part of the short arm of one of the X chromosomes. Mean±standard deviation (SD) height of the patients was 164.78±8.0cm. Five patients had decreased secondary sexual characteristics, and three patients had gynaecomastia with varying degrees. Five of the seven patients revealed a translocation between protein kinase X (PRKX) and inverted protein kinase Y (PRKY) genes, and the remaining two patients showed a translocation between the pseudoautosomal region 1 (PAR1) of X chromosome and the differential region of Y chromosome. X chromosome inactivation (XCI) analysis results demonstrated random and skewed XCI in 5 cases and 1 case, respectively. In brief, we delineate the phenotypic spectrum of patients with SRY-positive 46,XX TDSD and the underlying mechanisms of Xp;Yp translocations.

Moinha de carvão e bioestimulante na formação de mudas de galphimia brasiliensis

A triális (Galphimia brasiliensis) é uma planta originária da América do Sul, utilizada em paisagismo. Devido seu crescimento lento, a adoção de tecnologias, como a adição de moinha de carvão ao substrato e a aplicação de estimulantes vegetais, pode auxiliar no crescimento e na qualidade das mudas formadas. Assim, o objetivo deste trabalho foi avaliar a influência da adição de moinha de carvão e de bioestimulante na formação e qualidade de mudas. O delineamento experimental utilizado foi blocos casualizados, em esquema fatorial 5x2, testando proporções (0%; 7,5%; 15%; 22,5% e 30%) de moinha de carvão adicionadas a um substrato comercial, na presença (25 mL L-1) ou ausência de bioestimulante vegetal, com quatro repetições. Foram analisados os parâmetros: altura, comprimento de raiz, área foliar, diâmetro de colo e, massa seca de raiz, parte aérea e total. Com base nesses parâmetros foi avaliada a qualidade das mudas, pelas relações: altura/diâmetro (RHD), parte aérea/raiz (RPAR), altura/parte aérea (RHPA) e o Índice de qualidade de Dickson (IQD). O uso combinado de moinha de carvão e bioestimulante influenciou apenas a produção de massa seca de raiz das plantas de G. brasiliensis. A adição de bioestimulante não influenciou na formação e na qualidade das mudas de G. brasiliensis. A moinha de carvão adicionada ao substrato em proporções variando de 12,2 a 13,4% proporcionaram incremento na altura das mudas, na massa seca de raiz e total. Proporções crescentes após 18,6% de moinha de carvão, no intervalo testado, teve um acréscimo na relação parte aérea e raiz e no índice de qualidade de Dickson.

Enhancement of antimicrobial properties of shoe lining leather using chitosan in leather finishing

In this study, a chitosan based coating method was developed and applied on the shoe lining leather surface for evaluating its inhibition to bacterial and fungal attacks. At first, chitosan was prepared from raw prawn shells and then the prepared chitosan solution was applied onto the leather surface. Secondly, the characterization of the prepared chitosan and chitosan treated leather was performed by solubility test, ATR-FTIR, XRD pattern, SEM and TGA. Evaluation of antimicrobial efficacy of chitosan was assessed against two gram positive, two gram negative bacteria and a reputed fungi by agar diffusion test. The results of this study demonstrated that chitosan took place in both the surface of collagen fibres and inside the collagen matrix of crust leather. The chitosan showed strong antimicrobial activities against all the tested microorganisms and the inhibition increased with increasing percentage of chitosan. Therefore, the prepared chitosan in this study can be an environment friendly biocide, which functions simultaneously against different spoilage bacteria and fungi on the finished leather surface. Thus by using the prepared chitosan in shoe lining leather, the possibility of microbial attack during shoe wearing can be minimized which is one of the important hygienic requirements of footwear.

THRESHOLDS FOR TESTICULAR SIZE DISCREPANCY IN FERTILE MEN WITH AND WITHOUT VARICOCELE

The difference in testicular sizes between right and left testes is often utilized to determine the effect of pathology such as varicoceles on testicular growth. Furthermore, the normal testicular size differential in adult fertile men is currently unknown. We sought to characterize the testicular size distribution in fertile men with and without clinical varicoceles.

Application of novel pH sensitive isoniazid-heptamethine carbocyanine dye conjugates against prostate cancer cells.

Recent studies have shown that monoamine oxidase A (MAOA) is significantly expressed in malignant prostate cancer (PCa) and plays an important role in tumorigenesis indicating its potential to serve as a target for PCa treatment. Here, we choose the small molecule isoniazid as the MAOA inhibition functionality and incorporated it in the tumor-targeting moiety of heptamethine carbocyanine dyes via a pH sensitive hydrazone bond to design and synthesize novel MAOA inhibitor isoniazid-heptamethine carbocyanine dye conjugates. Cytotoxicity assay in PC-3 cells shows that all conjugates possessed improved antitumor efficacy compared with isoniazid. The tested compounds also demonstrated a moderate MAOA inhibitory effect. In conclusion, these results indicate that these conjugates exert antitumor effects by delivering the MAOA-inhibiting moiety to PCa cells.

Testicular blood supply is altered in the 41,XXY* Klinefelter syndrome mouse model

Hypergonadotropic hypogonadism is a major feature of Klinefelter syndrome (KS), assumed to be caused by testicular hormone resistance. It was previously shown that intratesticular testosterone levels in vivo and Leydig cell function in vitro seem to be normal indicating other functional constraints. We hypothesized that impaired testicular vascularization/blood flow could be a co-factor to the observed hypergonadotropic hypogonadism. We evaluated the testicular vascular system by measuring blood vessel sizes during postnatal development and testis blood flow in adult 41,XXY* mice. Proportional distribution and size of blood vessels were analyzed during testicular development (1, 3, 5, 7, 10, 21 dpp, 15 wpp). While ratios of the vessel/testis area were different at 15 wpp only, a lower number of smaller and mid-sized blood vessels were detected in adult KS mice. For testicular blood flow determination we applied contrast enhanced ultrasound. Floating and reperfusion time for testicular blood flow was increased in 41,XXY* mice (floating: XY* 28.8 ± 1.69 s vs XXY* 44.6 ± 5.6 s, p = 0.0192; reperfusion XY* 19.7 ± 2.8 s vs XXY*: 29.9 ± 6.2 s, p = 0.0134), indicating a diminished blood supply. Our data strengthen the concept that an impaired vascularization either in conjunction or as a result of altered KS testicular architecture contributes to hormone resistance.

Mass Spectrometry Supports That the Structure of Circulating Human Insulin-Like Factor 3 Is a Heterodimer

The structure of the testicular peptide hormone insulin-like factor 3 (INSL3) has been the subject of discussion for more than a decade. Some studies support that the central C-domain of INSL3 is proteolytically removed and that INSL3 is secreted by the testicular Leydig cells into circulation as a small heterodimer consisting of an A- and a B-chain linked by two disulfide bridges. Other studies support that the INSL3 peptide remains uncleaved and that the predominant structure of circulating INSL3 is the larger pro-form. Furthermore, the structure of INSL3 could differ between species, and both structural forms of INSL3 could, in principle, be present in circulation. Recently, we have developed a mass spectrometry (MS)-based method for INSL3 in human serum that provides new information about the structure of circulating INSL3. Based on recent and newly presented data, we argue that in healthy men, the common, and probably the only, form of circulating INSL3 is the smaller AB heterodimer. For the first time, we demonstrate that the same analytical principle, with slight modifications, can also be applied to sera from other species, and we show that the INSL3 AB heterodimer is also present in serum from rodents. Improved understanding of the structure and biochemistry of circulating INSL3 could be valuable for the interpretation of INSL3 as a marker for reproductive and developmental disorders in humans and domesticated animals.

Allelopathy Effect of Trichoderma spp. and Some Plant Extracts against Pythium aphanidermatum (In-vitro)

Background: Serious seed-borne and soil-borne pathogens are existing at the rhizosphere area, many species of Pythium can cause seed rot and damping-off disease. The application of plant extracts and biological agents as alternative ways for the management of plant diseases has recently become an important variable component for Integrated Pest Management, so plant metabolites regarded as eco-friendly where plants play an important role in the control of diseases. The current study amid to evaluate the allelopathy effect of two species of biological agents particularly Trichoderma spp. and some plant extracts against the soil-borne pathogen P. aphanidermatum on cucumber seedlings under laboratory conditions. Methods: In this study laboratory investigation during 2018, used the allelopathy effect of two species of Trichoderma (T. harzianum and T. hamatum) and five plant extracts, in Three conc. 10.000, 20.000 and 30.000 ppm, to control damping-off disease in cucumber caused by Pythium aphanidermatum instead of using chemicals fungicides. Result: Our study observed that Trichoderma spp., have a high inhibitory effect on the tested pathogen, but in the assay of plant extracts, only some with different concentrations had a significant effect against P. aphanidermatum (in-vitro). Cinnamomum cassia, Syzygium aromaticum and Eucalyptus globulus were most affective from all other five tested plant extracts, where the concentration of 30.000 ppm gave the highest inhibitory effect against P. aphanidermatum, while Psidium guajava and Lawsonia inermis extracts showed a lower inhibitory effect on radial growth of P. aphanidermatum.

Suppression of myofilament cross-bridge kinetic in the heart of orchidectomized rats

AimsThe increased incidence of heart failure with reduced ejection fraction in men compared with women suggests that male sex hormones significantly impact myocardial contractile activation. This study aims to examine associations among molecular alterations, cellular modulations and in vivo cardiac contractile function upon deprivation of testicular hormones. Main methodsMyocardial structure and functions were compared among sham-operated control and twelve-week orchidectomized (ORX) male rats with and without testosterone supplementation. Key findingsEchocardiography and pressure-volume relationships demonstrated a decreased left ventricular ejection fraction compared with sham-operated controls. The percentage of contractility reduction was generally similar to the decrease in tension development detected in both right ventricular trabeculae and skinned isolated left ventricular cardiomyocytes of ORX rats. Reductions in tension cost and the rate constant of tension redevelopment (ktr) in ORX samples suggested a decrease in the rate of cross-bridge formation, reflecting a reduced number of cross-bridges. Slow cross-bridge detachment in ORX rat hearts could result from a shift of myosin heavy chain isoforms towards a slower ATPase activity β-isoform and reductions in the phosphorylation levels of cardiac troponin I and myosin binding protein-C. All the changes in the ORX rat heart, including ejection fractions and myofilament protein expression and phosphorylation, were completed attenuated by a physiological dose of testosterone. SignificanceTestosterone plays a critical role in regulating the mechanical and contractile dynamics of the heart. Deprivation of male sex hormones cause the loss of normal preserved cardiac contractile function leading to a high risk of severe cardiomyopathy progression.

The effect of lipid disturbance and vitamin D on the fertility in male albino rats

ABSTRACT Background: Vitamin D has multiple biological effects on male reproductive system. Vitamin D deficiency (VD−) and disturbance of lipid metabolism can induce changes in testicular hormone production and seminal parameters that relate to male infertility. Objective: To investigate the role of vitamin D and lipid metabolism on fertility in male albino rats. Methods: The study was performed on 60 male rats, divided into 6 groups; GI: control group, GII: orlistat group, GIII: orlistat and vitamin D group, GIV: hyperlipidemic group, GV: hyperlipidemic orlistat group and GVI: hyperlipidemic orlistat and vitamin D group. Serum total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-c) and high density lipoprotein cholesterol (HDL-c), 25-hydroxyvitamin D (25(OHD), testosterone (T), inhibin B, follicle-stimulating hormone (FSH) and estradiol (E) levels were determined. Sperm count and viability were also analyzed. Results: Administration of orlistat in GII caused significant alterations of the serum lipid profile, compared to control group. There was interaction between dietary fat and VD− on serum 25 (OHD). A significant VD− was occurred in both GII and GIV, compared to GI. VD− in these groups consequently caused significant decrease in serum T, inhibin B, sperm count and viability and significant increase in FSH and E levels. Administration of orlistat in GV caused significant changes in serum lipid profile, a decrease in E, increase in 25(OHD) and inhibin B, compared to IV. On the other hand, administration of vitamin D with orlistat in GIII and GVI caused significant increase in HDL-c, 25(OHD), T, sperm count and viability and significant decrease in TC, TG, LDL-c, FSH and E. Conclusion: Both hyperlipidemia and hypolipidemia were associated with vitamin D deficiency. Moreover, vitamin D has a positive potential effect on male fertility in either hyperlipidemic or hypolipidemic rats.

46, XX male syndrome- testicular disorder of sexual differentiation

Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

Kolaviron suppresses dysfunctional reproductive axis associated with multi-walled carbon nanotubes exposure in male rats.

Reproductive toxicity associated with excessive exposure to multi-walled carbon nanotubes (MWCNTs), which are commonly used in medicine as valuable drug delivery systems, is well documented. Kolaviron, a bioflavonoid isolated from Garcinia kola seeds, elicits numerous health beneficial effects related to its anti-inflammatory, anti-genotoxic activities, anti-apoptotic, and antioxidant properties. However, information on the role of kolaviron in MWCNTs-induced reproductive toxicity is not available in theliterature. Herein, we assessed the protective effects of kolaviron on MWCNTs-induced dysfunctional reproductive axis in rats following exposure to MWCNTs (1mg/kg) and concurrent treatment with kolaviron (50 or 100mg/kg body weight) for 15 successive days. Results showed that MWCNTs-induced dysfunctional reproductive axis as evidenced by deficits in pituitary and testicular hormones, marker enzymes of testicular function, and sperm functional characteristics were abrogated in rats co-administered with kolaviron. Moreover, co-administration of kolaviron-abated MWCNTs-induced inhibition of antioxidant enzyme activities increases in oxidative stress and inflammatory indices. This is evidenced by diminished levels of tumor necrosis factor-alpha, nitric oxide, lipid peroxidation, reactive oxygen, and nitrogen species as well as reduced activity of myeloperoxidase in testes, epididymis, and hypothalamus of the rats. Biochemical data on the chemoprotection of MWCNTs-induced reproductive toxicity were corroborated by histological findings. Taken together, kolaviron suppressed dysfunctional reproductive axis associated with MWCNTs exposure via abrogation of oxidative stress and inflammation in male rats.