Effect of dietary supplementation of omega-3 rich oils on seminal characteristics, oxidative stress and plasma testosterone levels in Beetal bucks

Abstract

The objective of the present study was to evaluate the efficacy of dietary supplementation of omega-3 rich oils, chia seed oil (CSO; Salvia hispanica) and linseed oil (LSO; Linum usitatissimum), on seminal characteristics, and plasma testosterone levels in Beetal bucks. The bucks were fed diet supplemented with either LSO or CSO @1% on dry matter basis or un- supplemented diet for 100 days. Daily intake of omega-3 fatty acids was 0.60 (control diet), 4.04 (diet supplemented with LSO) and 4.57 g (diet supplemented with CSO). Ultrasonography revealed no significant improvement in the testicular size yet the growth rate was much high in animals fed omega-3 supplemented diet. Ejaculate volume, individual motility, viability, sperm concentration, plasma membrane and acrosome integrity were significantly higher in bucks fed CSO supplemented diet as compared to LSO and control diet. The percentage of abnormal spermatozoa was significantly less in bucks fed CSO and LSO diet than control. Melanoaldehyde (MDA, μmole/109 sperm) concentration was lowest in bucks fed CSO diet followed by LSO and control diet. Activity of superoxide dismutase, glutathione reductase and catalase was significantly higher in spermatozoa of bucks fed CSO and LSO diet as compared to control. Bucks fed CSO and LSO supplemented diet had nonsignificantly higher plasma testosterone concentrations than control. These findings indicated that CSO supplementation in the diets may be helpful in improving the process of spermatogenesis, sperm output, quality and fertility of bucks.