Stutzeri Strain Research Articles

Determination of the genetic dimension of Pseudomonas stutzeri which of nitrogen-fixation bacteria in a random PCR method

Bacteria play an important role in fixing atmospheric nitrogen and converting it into compounds that can be used by plants. Due to the importance of this topic, this type of bacteria was chosen, and its genetic dimension was studied. The nitrogen-fixing strain of Pseudomonas stutzeri, or those that have already been positively identified, should be collected from the wild or from laboratory cultures. Pseudomonas stutzeri strain was collected and their genomic DNA was extracted. This can be done using regular methods for extracting DNA. The purpose of this research is to generate a diverse panel of PCR amplification primers. Primers chosen at random are short sequences of nucleotides that can bind to various locations in the bacterial genome. These primers can be used to initiate polymerase chain reaction (RPCR) amplification at a high number of random positions within the DNA, allowing researchers to probe a wide range of genes. Fifteen different bundles, ranging in size from 200 to 3000 base pairs, were recorded. Thirteen differential bundles of 350, 425, 600, 650, 700, 800, 950, 1000, 1300, 1400, 1500, 1700, and 3000 base pairs are featured by the inclusion of two public bundles of 200 and 2000. Fifteen different bundles, ranging in size from 200 to 3000 base pairs, were recorded. Thirteen differential bundles of 350, 425, 600, 650, 700, 800, 950, 1000, 1300, 1400, 1500, 1700, and 3000 base pairs are featured by the inclusion of two public bundles of 200 and 2000.

Complete genome sequence of iron-oxidizing Stutzerimonas stutzeri strain FeN3W isolated from Catalina Harbor sediment.

Stutzerimonas stutzeri strain FeN3W is an iron-oxidizing bacterium isolated from marine sediment. FeN3W's 5.9 Mb genome encodes complete pathways for glycolysis, gluconeogenesis, TCA cycle, pentose phosphate pathway, and aerobic and anaerobic (nitrate) respiration. The genome contains 32 putative heme-binding proteins predicted to localize to the cell envelope.

Enhanced chromium and nitrogen removal by constructing a biofilm reaction system based on denitrifying bacteria preferential colonization theory

Understanding the developmental characteristics of microbial communities in biofilms is crucial for designing targeted functional microbial enhancements for the remediation of complex contamination scenarios. The strong prioritization effect of microorganisms confers the ability to colonize strains that arrive first dominantly. In this study, the auto-aggregating denitrifying bacterial Pseudomonas stutzeri strain YC-34, which has both nitrogen and chromium removal characteristics, was used as a biological material to form a stable biofilm system based on the principle of dominant colonization and biofortification. The effect of the biofilm system on nitrogen and chromium removal was characterized by measuring the changes in the quality of influent and effluent water. The pattern of biofilm changes was analyzed by measuring biofilm content and thickness and characterizing extracellular polymer substances (EPS). Further analysis of the biofilm microbiota characteristics and potential functions revealed the mechanism of strain YC-34 biofortified biofilm. The results revealed that the biofilm system formed could achieve 90.56% nitrate-nitrogen removal with an average initial nitrate-nitrogen concentration of 51.9 mg/L and 40% chromium removal with an average initial hexavalent chromium Cr(VI) concentration of 7.12 mg/L. The biofilm properties of the system were comparatively analyzed during the biofilm formation period, the fluctuation period of Cr(VI)-stressed water quality, and the stabilization period of Cr(VI)-stressed water quality. The biofilm system may be able to increase the structure of hydrogen bonds, the type of protein secondary structure, and the abundance of amino acid-like components in the EPS, which may confer biofilm tolerance to Cr(VI) stress and allow the system to maintain a stable biofilm structure. Furthermore, microbial characterization indicated an increase in microbial diversity in the face of chromium stress, with an increase in the abundance of nitrogen removal-associated functional microbiota and an increasing trend in the abundance of nitrogen transfer pathways. These results demonstrate that the biofilm system is stable in nitrogen and chromium removal. This bioaugmentation method may provide a new way for the remediation of heavy metal-polluted water bodies and also provides theoretical and application parameters for the popularization and application of biofilm systems.

Whole genome sequence of denitrifying bacterium Stutzerimonas stutzeri strain NGHE31, collected from an eutrophic wetland in Sunamganj, Bangladesh, following the 2017 flash floods.

Here, we report the whole genome sequence of Stutzerimonas stutzeri strain NGHE31, isolated from Dekhar Haor, following the 2017 flash flood that resulted in mass die-offs of local wildlife. The predicted genome size is 4,434,670 bp, with 63.97% GC content, 4,035 coding sequences, 3 rRNAs, and 50 tRNAs.

Application of response surface Methodology coupled with Artificial Neural network and genetic algorithm to model and optimize symbiotic interactions between Chlorella vulgaris and Stutzerimonas stutzeri strain J3BG for chlorophyll accumulation

Research on microalgae has surged due to its diverse biotechnological applications and capacity for accumulating bioactive compounds. Despite considerable advancements, microalgal cultivation remains costly, prompting efforts to reduce expenses while enhancing productivity. This study proposes a cost-effective approach through the coculture of microalgae and bacteria, exploiting mutualistic interactions. An engineered consortium of Chlorella vulgaris and Stutzerimonas stutzeri strain J3BG demonstrated biofilm-like arrangements, indicative of direct cell-to-cell interactions and metabolite exchange. Strain J3BG's enzymatic characterization revealed amylase, lipase, and protease production, sustaining mutual growth. Employing Response Surface Methodology (RSM), Artificial Neural Network (ANN), and Genetic Algorithm (GA) in a hybrid modeling approach resulted in a 2.1-fold increase in chlorophyll production. Optimized conditions included a NaNO3 concentration of 128.52 mg/l, a 1:2 (Algae:Bacteria) ratio, a 6-day cultivation period, and a pH of 5.4, yielding 10.92 ± 0.88 mg/l chlorophyll concentration.

Silver, Its Salts and Application in Medicine and Pharmacy

The healing properties of silver have been used since ancient times. The main aim of the study was to collect and review the literature on the clinical potential of silver, its salts and complex compounds. The second goal was to present an outline of the historical use of silver in medicine and pharmacy, taking into account the possibility of producing pharmaceutical drug forms on the premises of pharmacies. In the context of the growing resistance of microorganisms to available, widely used antibiotics, silver plays a key role. There is only one known case of bacterial resistance to silver—the Pseudomonas stutzeri strain, which naturally occurs in silver mines. The development of research in the field of coordination chemistry offers great opportunities in the design of new substances in which silver ions can be incorporated. These substances exhibit increased potency and often an extended antimicrobial spectrum. Silver-based compounds are, however, only limited to external applications, as opposed to their historic oral administration. Advanced studies of their physicochemical, microbiological, cytotoxic and genotoxic properties are ongoing and full of challenges. The improvement of the methods of synthesis gives the possibility of applying the newly synthesized compounds ex tempore, as was the case with the complex of metronidazole with silver (I) nitrate. Some of these experimental efforts performed in vitro are followed with clinical trials. The third and final goal of this study was to present the possibility of obtaining an ointment under the conditions of an actual pharmacy using silver (I) salts and a ligand, both of which are active substances with antimicrobial properties.

The Mass Spectrometry Identification, Antimicrobial Genes Detection, and Proteomics Analysis of Stutzerimonas stutzeri Strain Was Isolated from Industrial Wastewater

A large amount of organic matter, heavy metals, and even antibiotics are present in industrial wastewater, aquaculture waters, and various types of sewage, along with abundant microorganisms. To date, only a few studies involving the resistance and proteomics of Stutzerimonas stutzeri in high-salt wastewater are available. Herein, a comprehensive assessment of a newly isolated Stutzerimonas stutzeri strain, which is present in high-salt wastewater, was performed using mass spectrometry, genetic identification, and biochemical analysis to characterize the genetic and biochemical properties. Growth experiments revealed that the Stutzerimonas stutzeri strain had a moderate growth rate in nutrient broth, and the bacterial count was not high. Further analysis highlighted an apparent susceptibility of this strain to most antibiotics but some resistance to chloramphenicol and minocycline. A resistance gene assay results showed that the gene gyrB was associated with antibiotic resistance in this Stutzerimonas stutzeri strain. Proteomic analysis revealed for the first time the co-existence of two drug-resistance-related proteins (Multidrug/solvent RND membrane fusion protein and MexE) in Stutzerimonas stutzeri. Moreover, Stutzerimonas stutzeri isolated from high-salt wastewater was subjected to drug resistance gene detection, and the total protein of Stutzerimonas stutzeri was detected by protein mass spectrometry analysis. The subcellular classification shows that the 50 proteins with the highest abundance are divided into cell inner membrane, cell outer membrane, cytoplasm, cytoplasmic side, membrane, multi-pass membrane protein, and peripheral membrane protein, among which the proportion of cytoplasmic components is the highest. Overall, this study’s findings provide a new perspective for further research on the characteristics of Stutzerimonas stutzeri in high-salt wastewater.

Growth Optimization of Naphthalene-Degrading Pseudomonas Stutzeri Strain BUK_BTEG1 Isolated from Petrochemical Contaminated Soil.

Bioremediation of PAHs such as naphthalene by the activity of microorganisms represents an eco-friendly, economical, and safe decontamination approach compared physical and chemical processes. In this study, the biodegradation conditions for naphthalene by Pseudomonas stutzeri strain BUK_BTEG1 was optimized through onfactor-at-a-time (OFAT). The Characterization was conducted by studying the effect of incubation time, nitrogen source, pH, inoculum size, substrate concentration and temperature in BH media containing naphthalene as sole source of carbon and energy. The growth and degradation of naphthalene by this strain was optimal at 1000 mg/L ammonium Nitrate, substrate concentration of 800mg/L, temperature of 35 °C, pH of 7.0, inoculum size of 4% (v/v) and 72 hours of incubation time. After 72 hours of incubation under optimal conditions, the degradation efficiency of this strain to naphthalene was 72%. The analysis using gas chromatography-mass spectrometry reveals 1,2-naphthalenediol, 1-hydroxy-napthoic acid, 1,4-Napthoquinone, salicylic acid, and catechol as metabolites of naphthalene degradation by comparing the fragmentation pattern and mass spectrum obtained with data available on NIST library. This strain demonstrates promising potential and could be handy for its application in the bioremediation of naphthalene contaminated sites.

Ovezanost koronarne ateroskleroze sa sojevima stutzerimonas stutzeri (HaSa 1, 2, 3 i 4) bakterijama koje formiraju biofilm

Objective. Coronary atherosclerosis, also known as coronary artery disease, is increasing in developing countries like Iraq. A recent study investigated the presence and potential impact of a bacterial strain called Stutzerimonas stutzeri on coronary atherosclerosis. This research aims to provide insights into this medical concern that has seen a significant surge in cases over the past two decades. Methods. Samples were collected from patients who underwent Percutaneous Coronary Intervention (PCI) at the Mosul Center for Cardiology and Cardiac Surgery between 9/10/2022 and 1/3/2023. The samples were obtained from the fluid (blood) coming out from the guiding catheter of the balloon during angioplasty, and then the balloon samples were placed in transport media (Tryptone Soya Broth). Advanced scientific methods, including biochemical tests, the Vitek-2 system, a Scanning Electron Microscope (SEM), and Molecular methods based on the 16S rRNA gene, were utilized to identify the bacteria. The Nitrogen base sequences were located and compared to those in the NCBI database using BLAST software. Results. New strains of Stutzerimonas stutzeri bacteria were discovered in people with coronary atherosclerosis for the first time. The National Center for Biotechnology Information (NCBI) named these new strains as follows: Stutzerimonas stutzeri strain HaSa1, S. stutzeri strain HaSa2, S. stutzeri strain HaSa3, and S. stutzeri strain HaSa4. They have the ability to form biofilms. This was diagnosed with direct smears of balloon samples, smears of isolated bacterial colonies stained with a gram stain, and scanning electron microscope photos of balloon samples. Conclusion. The newly found Stutzerimonas stutzeri strains most likely cause chronic inflammatory responses in people that have atherosclerosis in their coronary arteries by building biofilms.

Suppression of Fusarium Wilt of Chickpea by Bacillus spp., Pseudomonas sp. and Rhizobacterial Isolate

This experiment was conducted to evaluate the antagonistic effects of some selected rhizobacteria on Fusarium oxysporium f. sp. ciceris in a pot experiment. Rhizobacterial isolates (one isolate of Pseudomonas, eight isolates of Bacillus genera and one bacterium isolate) and two chickpea cultivars (Shendi and Burgeig) were arranged in a factorial pot experiment in CRD with four replicates. The disease incidence and severity were detected weekly. Disease reduction percentage was estimated at the end of the study. Generally, the application of rhizobacterial isolates as biological control agent reduced disease incidence compared with the control in both cultivars. The incidence in cultivar Shendi occurred at the third week after inoculation when treated with Pseudomonas stutzeri strain W28 (SA3) and Bacillus subtilis strain CM14(SA9). For the two cultivars, Shendi and Burgeig, the Geobacillus sp. CRRI-HN-1(SA2) and Bacillus sp (SA1), respectively had the highest positive effect on disease incidence and severity throughout the experiment compared with the control. These were 45.36 and 44.82% in incidence; 55.36 and 63.89% in severity, respectively.

Role of Ca2+ and Mg2+ in changing biofilm structure and enhancing biofilm formation of P. stutzeri strain XL-2

The aim of this study was to elucidate the role of Ca2+/Mg2+ in changing biofilm structure and enhancing biofilm formation of P. stutzeri strain XL-2. It was found that Ca2+/Mg2+ stimulated the production of extracellular polymers substances (EPS). More specifically, Ca2+ mainly promoted the secretion of proteins in EPS, while Mg2+ promoted polysaccharides. The stimulation of EPS secretion was essential to promote the biofilm formation of strain XL-2. By the analysis of Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, Ca2+ mainly interacted with the amide groups in proteins of EPS, and Mg2+ was able to interact with polysaccharides as well as proteins. This interaction between Ca2+/Mg2+ and specific functional groups in EPS acted as a bridge between the EPS matrix, which also promoted the biofilm formation and made the biofilm structure more compact. Besides, the charge neutralization and electric double layer compression caused by Ca2+/Mg2+ participated in the formation of biofilm to a certain extent.

Maize Growth Promotion by Inoculation with an Engineered Ammonium-Excreting Strain of Nitrogen-Fixing Pseudomonasstutzeri.

Diazotroph mutants designed using metabolic engineering to excrete surplus ammonium were used to enhance nitrogen fixation and plant growth, as the levels of nitrogen fixation attained with diazotrophs are insufficient for the plant’s needs. In this study, wild-type (A1501) and engineered ammonium-excreting (1568/pVA3) strains of nitrogen-fixing Pseudomonas stutzeri strains were tested in vitro based on plant growth-promoting traits, such as phosphate solubilization ability, indole acetic acid (IAA) production and nitrogenase activities, as well as ammonium excretion as affected by mannitol-mediated osmotic stress. The maize plant growth-promoting effect of the A1501 and 1568/pVA3 strains was evaluated in pots and in the field, and the 15N-dilution technique was employed to assess the proportion of plant nitrogen derived from nitrogen fixation. The results demonstrate that the 1568/pVA3 strain displayed higher IAA production and nitrogenase activity than A1501 and released significant quantities of ammonium. After 50 days, in all of the conditions assayed, maize inoculated with 1568/pVA3 accumulated more plant biomass (3.3% on average) and fixed N (39.4% on average) than plants inoculated with A1501. In the field experiment, the grain yield of maize was enhanced by 5.6% or 5.9% due to the inoculation of seeds with 1568/pVA3 in the absence or presence of exogenous N fertilizer, respectively. Therefore, the engineered P. stutzeri strain tested in the greenhouse and field was shown to perform better than the wild-type strain with respect to maize growth parameters and biologically fixed nitrogen.

Rapid method for detection, quantification and measuring microbial degradation of pesticide-thiram using high performance thin layer chromatography (HPTLC).

Thiram (tetramethylthiuramdisulfide) or thiram sulphide is a dithiocarbamate group of non-systemic group of fungicide which are applied for seed treatment, control of the crop pests, to repel animals, etc. Moreover, thiram has also been responsible to cause moderate skin sensitivity and eye irritation. Higher exposure to thiram might also lead to developmental damages to newborn and neurotoxic effects to non-target organisms. Advancing to prevent such toxic effects and prevention of soil fertility from thiram and thiram-like chemicals is indispensable. The analytical High-Performance Thin-Layer Chromatography (HPTLC) is a simple, quick and a reliable method was proposed and validated for the detection and quantification of various small molecules for many years. This manuscript represents the solution to use microbes to degrade the thiram present in the soil and for that, HPTLC based method to study thiram degradation by Pseudomonas has been designed. Herein, a HPTLC protocol formalised to reveal the detection and quantification of thiram within the range of 100 to 700ng/spot on TLC plate. The same concentration was then used for calculating percent microbial degradation of thiram from the culture broth. To perform the microbial degradation of thiram, Pseudomonas otitidis strain TD-8 and Pseudomonas stutzeri strain TD-18 were taken as thiram degrader microbial strain. The efficacy of TD-8 to degrade thiram was identified to be 81 and 99% when grown in presence of thiram for 4days and 8days, respectively, while TD-18 strain's efficacy to degrade thiram was found to be 57% and 99% when grown in presence of thiram for 4days and 8days, respectively.

Even allocation of benefits stabilizes microbial community engaged in metabolic division of labor.

Microbial communities execute metabolic pathways to drive global nutrient cycles. Within a community, functionally specialized strains can perform different yet complementary steps within a linear pathway, a phenomenon termed metabolic division of labor (MDOL). However, little is known about how such metabolic behaviors shape microbial communities. Here, we derive a theoretical framework to define the assembly of a community that degrades an organic compound through MDOL. The framework indicates that to ensure community stability, the strains performing the initial steps should hold a growth advantage (m) over the "private benefit" (n) of the strain performing the last step. The steady-state frequency of the last strain is then determined by the quotient of n and m. Our experiments show that the framework accurately predicts the assembly of our synthetic consortia that degrade naphthalene through MDOL. Our results provide insights for designing and managing stable microbial systems for metabolic pathway optimization.

Statistical design for biogenesis of melanin nanoparticles from producer strain Pseudomonas stutzeri BTCZ 109 through Taguchi DOE

Melanins are a group of biological macromolecules synthesized by a wide spectrum of living organisms mainly through oxidative polymerization of indolic and phenolic compounds. Because of its various properties including antioxidation, antibiofilm, metal chelation, UV protection and anticancer activity it has widespread application in agriculture, cosmetic and therapeutic industries which makes it commercially valuable and its large-scale production attain enormous consideration. The phylogenetic analysis reveals that the organism belongs to Pseudomonas stutzeri strain (BTCZ 109). Taguchi method of design of experiment (DOE) using Qualitek-4 software for the optimization of parameters to enhance melanin production was carried out in this study. L8 (27) type of Orthogonal Arrays (OA) was constructed using 7 variables including incubation time (days), inoculum concentration (%), pH, MgSO4.5H2O (mM), l-tyrosine (g/l), temperature (0C) and agitation (rpm). Maximum melanin production through different combinations of experimental variables, factors contributing to production and interaction among the selected factors at their given levels, were elucidated. 8 trials of experiments were performed under the L-8 Orthogonal Array (OA) designed by the Taguchi approach. The experimental data were analyzed using the Signal-to-noise (S/N) ratio with the ‘bigger is better’ characteristics to identify the influential parameters and to establish the optimum conditions for maximizing melanin production. Validation of the predicted data with the confirmatory experiments revealed a nominal value of 1328.855 μg/mL, with an average performance of 1158.27 μg/mL, at the optimized condition of incubation time 4 days, pH 8, temperature 35 °C, agitation 140 rpm, and MgSO4.5H2O (mM) 0.25 mM absorbance units (AU) of OD400. The melanin thus obtained was found to be nano-sized and which reveals its pharmaceutical, biomedical and environmental application.

Characterization of the facultative anaerobic Pseudomonas stutzeri strain HK13 to achieve efficient nitrate and nitrite removal

A new facultative anaerobic denitrifier was isolated from anoxic activated sludge and identified as Pseudomonas stutzeri strain HK13. Single-factor experiments indicated that the optimal conditions for nitrate removal were a C/N ratio of 8, an initial pH of 7–10, a shaking speed of 100 rpm, and a temperature of 30–40 °C. When 100 mg/L NO3--N and NO2--N were used separately as sole nitrogen sources, the strain exhibited efficient denitrification ability with total nitrogen (TN) removal efficiencies of 98.22% and 98.58%, respectively, in 15 h with the corresponding maximum removal rates of 20.03 and 21.77 mg/L/h. And there was no nitrite accumulation finally in the nitrate denitrification system. In addition, 97.42% total oxidized nitrogen (TON) was removed at 20 °C and 93.46% NO3--N was reduced at 45 °C, suggesting that strain HK13 had a wide range of temperature and pH to adapt to the environment. Moreover, strain HK13 exhibited a notable ability to tolerate high nitrate loading (initial NO3--N > 450 mg/L) with a TON removal amount of over 200 mg/L/day. These findings demonstrate that strain HK13 is effective for nitrogen removal in wastewaters containing high concentrations of nitrate and nitrite.

The essential role of hydrophobic interaction within extracellular polymeric substances in auto-aggregation of P. stutzeri strain XL-2

To enrich the understanding of bio-aggregates formation, the mechanism of auto-aggregation is one of the concerns in wastewater treatment. The auto-aggregation ability by P. stutzeri strain XL-2 was mainly attributed to the self-secretion of extracellular polymeric substances (EPS). A comprehensive correlation analysis indicates that hydrophobic interaction within EPS played essential roles in auto-aggregation. To give insights into the underlying mechanisms, tightly bound EPS (TB-EPS), loosely bound EPS (LB-EPS) and soluble EPS (S-EPS) were characterized by colorimetry, hydrophobicity, enzymatic hydrolysis, FT-IR spectroscopy and XPS analysis, showing that proteins in TB-EPS comprised a high proportion of hydrophobic carbon-containing (C-containing) functional groups. Further amino acids analysis revealed that these C-containing functional groups might derive from the hydrophobic R groups of amino acids, which significantly affected the surface hydrophobicity of proteins in TB-EPS and thus promoted the auto-aggregation of strain XL-2. This work provides an in-deep understanding of the auto-aggregation mechanism by strain XL-2, which may enrich the understanding of microbial aggregates formation and lead to further application of strain XL-2 in wastewater treatment processes.

Kinetics Modelling of Pseudomonas stutzeri strain DN2 Growth Behaviour in Tributyltin Chloride

A predictive model was performed to describe Pseudomonas stutzeri strain DN2 growth behaviour in tributyltin chloride, using primary Modelling and a polynomial model as a secondary predictive model. In this investigation, data predicted using the modified Logistic (ML) was the most accurate. The Bias Factor (Bf) and Accuracy Factor (Af) values for the (ML) model were 1.39 and 1.51, indicating that the predictions were within a reliable range. The low RMSE value of 0.14, R2 and adj R2 (0.99) value closer to 1, showing that modified logistics is better than the other models at describing the growth behaviour of Pseudomonas stutzeri strain DN2 in toxic tributyltin chloride. Both the Aiba and Haldane models on the other hand, among the secondary model best fit the behaviours having low RMSE and MSE values and adjR2 value closer to 1. In this study, the primary and secondary kinetics of Pseudomonas stutzeri strain DN2 growth behaviour in tributyltin chloride was explored and it was shown in this study that the modified logistic and the Haldane models better suit the growth behavior of Pseudomonas stutzeri strain DN2 in tributyltin chloride. The parameters obtained from the modelling exercise will be very valuable in transferring the laboratory results to the field.

Biofilm Interaction Mapping and Analysis (BIMA) of Interspecific Interactions in Pseudomonas Co-culture Biofilms.

Pseudomonas species are ubiquitous in nature and include numerous medically, agriculturally and technologically beneficial strains of which the interspecific interactions are of great interest for biotechnologies. Specifically, co-cultures containing Pseudomonas stutzeri have been used for bioremediation, biocontrol, aquaculture management and wastewater denitrification. Furthermore, the use of P. stutzeri biofilms, in combination with consortia-based approaches, may offer advantages for these processes. Understanding the interspecific interaction within biofilm co-cultures or consortia provides a means for improvement of current technologies. However, the investigation of biofilm-based consortia has been limited. We present an adaptable and scalable method for the analysis of macroscopic interactions (colony morphology, inhibition, and invasion) between colony-forming bacterial strains using an automated printing method followed by analysis of the genes and metabolites involved in the interactions. Using Biofilm Interaction Mapping and Analysis (BIMA), these interactions were investigated between P. stutzeri strain RCH2, a denitrifier isolated from chromium (VI) contaminated soil, and 13 other species of pseudomonas isolated from non-contaminated soil. One interaction partner, Pseudomonas fluorescens N1B4 was selected for mutant fitness profiling of a DNA-barcoded mutant library; with this approach four genes of importance were identified and the effects on interactions were evaluated with deletion mutants and mass spectrometry based metabolomics.

Biodegradation of Anthracene and Phenanthrene by Pseudomonas stutzeri (BUK_BTEG1) Isolated from Petrochemical Contaminated Soil

Abstract The United States Environmental Protection Agency (USEPA) has identified 16 substances as priority polycyclic aromatic hydrocarbons (PAHs) that are harmful to humans, including anthracene and phenanthrene. These substances are pervasive pollutants introduced into the environment through anthropogenic and natural processes, causing ecological concerns and necessitating the quest for new strains capable of biodegrading these toxins. A novel strain of the genus Pseudomonas was isolated and molecularly identified based on partial 16S rRNA and phylogenetic analysis as Pseudomonas stutzeri strain BUK_BTEG1 from petrochemical contaminated soil. One factor at a time (OFAT) in Bushnell-Haas (BH) media was used to optimize the strain’s biodegradation conditions. The isolate could grow up to 600 mgL−1 and 400 mgL−1 of anthracene and phenanthrene as the sole carbon source at an optimum pH of 7.0 and 7.5 respectively, inoculum concentration of 4% (v/v), and temperature of 35°C during 72 hours of incubation. The strain could degrade phenanthrene and anthracene to a maximum of 99 and 72 percent, respectively, under ideal conditions. The breakdown products’ GC-MS analysis revealed the existence of the pathway’s main metabolites, catechol, salicylic acid, and derivatives of phthalic acid. The strain exhibits promising potential for use in the bio-cleansing of environments contaminated by PAHs.metabolites.