Abstract
A predictive model was performed to describe Pseudomonas stutzeri strain DN2 growth behaviour in tributyltin chloride, using primary Modelling and a polynomial model as a secondary predictive model. In this investigation, data predicted using the modified Logistic (ML) was the most accurate. The Bias Factor (Bf) and Accuracy Factor (Af) values for the (ML) model were 1.39 and 1.51, indicating that the predictions were within a reliable range. The low RMSE value of 0.14, R2 and adj R2 (0.99) value closer to 1, showing that modified logistics is better than the other models at describing the growth behaviour of Pseudomonas stutzeri strain DN2 in toxic tributyltin chloride. Both the Aiba and Haldane models on the other hand, among the secondary model best fit the behaviours having low RMSE and MSE values and adjR2 value closer to 1. In this study, the primary and secondary kinetics of Pseudomonas stutzeri strain DN2 growth behaviour in tributyltin chloride was explored and it was shown in this study that the modified logistic and the Haldane models better suit the growth behavior of Pseudomonas stutzeri strain DN2 in tributyltin chloride. The parameters obtained from the modelling exercise will be very valuable in transferring the laboratory results to the field.
Highlights
Organotin compounds are chemical compounds based on tin with hydrocarbon substituents
Statistical analysis Statistical significant difference between the models was calculated through various methods including the adjusted coefficient of determination (R2), accuracy factor (AF), bias factor (BF), Root-Mean-Square Error (RMSE) and corrected
The accuracy and statistical analysis of all the secondary kinetic models used revealed that Monod, Haldane, Aiba, Yano and Luong were able to fit the data, with both the Aiba and Haldane being the best model with low RMSE and AICc (-12) values, highest adjusted R2 values, closest to unity (1.0), (Table 4)
Summary
Organotin compounds are chemical compounds based on tin with hydrocarbon substituents. Tributyltin chloride is known to be very toxic to both prokaryotes and eukaryotes, whereas the mono-, di-, and tetra-organotins are either non-toxic or slightly poisonous, and appear to cause toxicity through interacting with membrane lipids [8,9]. Several mechanisms involved in tributyltin chloride resistance in bacteria include (i) exclusion of the compound from the cell mediated by the multidrug efflux pump (ii) degradation/metabolic utilization as a carbon source [11]; (iii) bioaccumulation into the cell without the compound being broken down [12] and (iv) unique morphological alteration in the form of long inter-connected chains of bacterial cells on exposure to TBTCl [13]. The primary and secondary kinetics of Pseudomonas stutzeri strain DN2 growth behaviour in tributyltin chloride was explored
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