State Structures Research Articles

DEVELOPMENT OF THE ROMAN CATHOLIC CHURCH IN ODESA REGION (LATE XX – BEGINNING OF XXI CENTURY)

The purpose of the study is to highlight certain aspects of the development of the Roman Catholic Church (RCC) in Odesa region in the context of its problems and achievements at the end of the 20th – beginning of the 21st century. The scientific novelty of the work lies in the fact that, for the first time, a comprehensive study of the peculiarities of the development of the RCC in Odesa region against the background of historical events of the late 20th – early 21st centuries is proposed. On the basis of archival documents, the main stages of the activity of the RCC in Odesa region in the specified period are considered. It was found that the first stage on the way of the revival of the Church fell on the 1990s. Its characteristic features were the return of buildings previously confiscated by the Soviet authorities to Catholic communities, their reconstruction and restoration. During this period, it was possible to create a network of religious communities adapted to the conditions of activity in the plane of a multi-confessional environment. It is indicated that this process was under the control of the Department for Religious Affairs, which operated under the Odesa Regional State Administration during 1996-2005. It has been proven that the second stage of development of the RCC began at the beginning of the 21st century, which was due to the arrival of Pope John Paul II in Ukraine. The main achievements of this period are analyzed: the foundation of the new Odesa-Simferopol diocese headed by Bishop Bronislav Bernatsky, the increase in the number of believers, the activation of educational and charity work, activities aimed at improving inter-religious relations in the region. The intellectual and religious ties of the RCC with representatives of state structures and the public have been clarified. In the conditions of full-scale Russian aggression, the RCC took an active position in supporting the population of Ukraine and providing them with appropriate humanitarian aid. Further studies of church history include research on the construction of Christian churches in the south of Ukraine.

Plunger gate effects on magneto transport in double-top gate spin-orbit devices

A propagation matrix method is used to investigate the quantum conductance of an n-type double-top gate device under a strong Rashba-Zeeman coupling interaction. The effects of a plunger gate potential and the total top gate length on the magneto conduction properties are investigated. Transcendental equations and analytical formulas are derived to accurately predict the resonance state and hole-like bound state structures. Furthermore, the effect of the distance of the two top gates from the plunger gate is examined for both symmetric and asymmetric structures. The analytical technique employed in this study provides an accurate and reliable approach for calculating the conductance of the quantum transport and is therefore beneficial for the design of n-type semiconductor devices.

Light-Triggered Enhancement of Fluorescence Efficiency in Organic Cages.

The fluorescence efficiency of excited molecules can be enhanced by many external factors. Here, we showcase a surprising phenomenon whereby light is used as a gating source to increase the fluorescence efficiency of organic cages composed of biphenyl subunits. We show that the enhancement of fluorescence is not due to structural changes or ground-state events. Cryo-fluorescence measurements and kinetic studies suggest a restriction of the phenyl-based structures in the excited state, leading to increased fluorescence, which is also supported by time-resolved measurements. Through computational calculations, we propose that the planarization of the biphenyl units within the cages contributes to emission enhancement. This phenomenon offers insights into the design of optoelectronic structures with improved fluorescence properties.

Seismic fragility analysis of subway station structure subjected to sequence-type ground motions

As a critical urban infrastructure, the subway station damaged in an earthquake not only leads to interruption of underground transportation, but may also result in serious casualties and economic losses. The current seismic design of underground structures only considers the mainshock effects but ignores the potential hazards of aftershocks. However, most mainshocks are often followed by aftershocks in a short period of time. In the past earthquakes, it was reported that the mainshock-damaged engineering structures suffered more serious structural damage and even complete collapse when subjected to aftershocks before restoration. This paper proposes a framework for the development of seismic fragility curves of underground structures subjected to sequence-type ground motions. Finite element models of a two-story, three-span subway station considering nonlinear dynamic soil-structure interaction (SSI) was established in this study. A comprehensive assessment of the seismic performance of the subway station structure under the sequence-type ground motions was conducted on the SSI model. Both the mainshock-aftershock fragility analysis of the intact structure and the aftershock fragility analysis of the mainshock-damaged structures were performed by combining the traditional seismic probability demand model and the back-to-back mainshock-aftershock probability demand model. The numerical results indicated that the impact of aftershocks on the seismic performance of subway station structures cannot be ignored. The aftershock fragility of the structure is highly dependent on the mainshock-damaged state. The failure probability of a severely mainshock-damaged subway station structure is much higher in the aftershocks than structures in other damage states. Besides, the probability of the mainshock-damaged structure transferring to a more severe level of damage state increases with the increase of the intensity of aftershock motions. For the subway station under sequence-type ground motions, the transition probability of one severer level of damage state is greater than 40 % for the 1.0 g peak ground acceleration of aftershocks and the transition probability of more than two severer level of damage state is less than 10 %.

ILLUSTRATIONS OF EXTENDED WORKING LIVES: WHAT INSIGHTS DO THE CONCEPTS OF EXCLUSION AND PRECARITY OFFER?

Abstract Amanda Grenier will act as the discussant for the set of papers in this symposia session, bridging insights from European research with North American perspectives. Situated as an attempt to bridge research findings with a larger critical practice of theory-building theory, she will explore what the concepts of exclusion and precarity offer our interpretations of these papers, as well as how the findings of each contribute to the state of knowledge on exclusion and precarity. She will begin with a brief clarification of the definitions and conceptual boundaries of social exclusion and precarity carried out in Europe and North America and situate key findings of the papers as an effort to theorize older workers lives in contemporary conditions. For example, the well-known concept and domains of social exclusion can be considered to offer ways of understanding processes of exclusion at the mezzo level, as exercised through policies, practices, and/or place. Illustrations from the papers will be linked with existing work on exclusion. She will then draw attention to the concept of precarity as a lens of analysis to connect the micro-level vulnerabilities experienced in the working lives of older people with the shifting social and state structures that form the backdrop for older people’s lives (and the systems within which European research is carried out). Specific examples will be drawn from the set of papers, and a moderated question and answer period will follow.

Fabricating epoxy composite coating having self-healing/barrier anti-corrosion functions utilizing ion-exchange/pH-sensitive phosphate-doped ZIF8 MOF decorated Zn-Al-LDH nano-layers

Recently, the development of epoxy composite coatings with smart anti-corrosion features has been considered in many studies. Using inhibitor-loaded nanocarriers, i.e., layered double hydroxides (LDH) and metal-organic frameworks (MOFs) has attracted recent attention. In this work, the hybrid LDH@ZIF8 was constructed and applied in the epoxy coating as a dual-functional nanocarrier.In this study, firstly, LDH nano-size lamellar was synthesized followed by surface modification with nano-porous ZIF8 3D structures. Afterward, to provide further corrosion inhibition properties, phosphate ions were doped and the resultant particles were introduced into the epoxy coating. Fourier transform infrared (FTIR) spectroscopy, scanning electron microscope (SEM), and X-ray diffraction (XRD) analysis were utilized to characterize the nanoparticles and they were a good indicator to prove the successful surface modification. Via inductively coupled plasma (ICP), the inhibitor pH/ion-responsive release (i.e., PO43− anions, Zn2+ cations) ability at various pHs was screened. Electrochemical measurements proved the inhibitive action of the phosphate-doped LDH@ZIF8 structures in the solution state (75.99 % inhibition index). EIS results revealed that after incorporating the phosphate-doped LDH@ZIF8 nano-hybrid into the epoxy coating its barrier properties were significantly improved (impedance values>1010 Ω.cm2). Besides, the phosphate-doped LDH@ZIF8 filled epoxy (with artificial scratch) showed about 57 % self-healing index. The adhesion bond durability was significantly improved after the addition of phosphate-doped LDH@ZIF8. About 69 % improvement in the wet adhesion (pull-off test) and 73 % reduction of cathodic delamination diameter (CD test) were recorded for the epoxy coating loaded with phosphate-doped LDH@ZIF8 compared to the blank epoxy. Generally, using phosphate-doped LDH@ZIF8, both barrier and active corrosion protection properties of the epoxy coating were significantly improved, and compared to similar works reported in literature better anti-corrosion durability was reached.

Theoretical Studies on the Isomerization Kinetics of Low-Lying Isomers of the SiC4H2 System.

The low-lying isomers of SiC4H2 are investigated to understand the kinetics of isomerization pathways using density functional theory. In our earlier work, we studied the various possible isomers (J. Phys. Chem. A, 2020, 124, 987-1002) and the chemical bonding of low-lying isomers of SiC4H2 (J. Phys. Chem. A, 2022, 126, 9366-9374). Among them, four isomers, 1-ethynyl-3-silacycloprop-1-en-3-ylidene (1), 3-silapent-1,4-diyn-3-ylidene (2), 1-silapent-1,2,3,4-tetraen-1-ylidene (4), and 1-silapent-2,4-diyn-1-ylidene (5) have already been identified in the laboratory. The previously known theoretical isomer 2-methylene-1-silabicyclo[1.1.0]but-1(3)-en-4-ylidene (3) and the newly identified unknown isomer through the present kinetic studies 5-silabicyclo[2.1.0]pent-1(4),2-dien-5-ylidene (N6) remain elusive in the laboratory to date. The isomerization pathways of the low-lying isomers of SiC4H2 are predicted through the transition state structures. Intrinsic reaction coordinate analysis identifies the minimum energy reaction pathways connecting the transition state from one isomer to another of the investigated system. The present kinetic data reveal the isomerization of global minimum energy isomer 1 to thermodynamically stable low-lying isomers, 2 and 5. Interestingly, isomer 3 interconverts to the experimentally known low-energy isomer 4, the second most thermodynamically stable isomer among them. The thermodynamic and kinetic parameters of the low-lying isomers of SiC4H2 are also documented in this work. The rate coefficient and equilibrium constant for isomerization reactions are calculated using the Rice-Ramsperger-Kassel-Marcus theory. The equilibrium constant delineates the difficulties in forming N6 and 3 through the isomerization pathways. Furthermore, ab initio molecular dynamics studies dictate the stability of low-lying isomers of SiC4H2 within the time scale of the simulation.

Predicting Binding Energies and Electronic Properties of Boron Nitride Fullerenes Using a Graph Convolutional Network.

As isoelectronic counterparts of carbon fullerenes, medium-sized boron nitride clusters also prefer cage structures composed of even-sized polygons. As the cluster size increases, the number of cage isomers grows rapidly, and determining the ground state structure requires a tremendous amount of DFT calculations. Herein, we develop a graph convolutional network (GCN) that can describe the energy of a (BN)n cage by its topology connection. We define a vertex feature vector on a dual polyhedron by the permutation of the neighbor vertices' degree and aggregate the information on vertices by two graph convolutional layers to learn the local feature of the dual polyhedron. The GCN is trained on (BN)28 and subsequently tested on (BN)23 and (BN)24 data sets, which satisfactorily reproduce the order of isomer energies from DFT calculations. We further employ the trained GCN to predict the ground state structures within the size range of n = 25-32, which agree well with DFT results. Using the same GCN framework, we also successfully trained the highest-occupied or lowest-unoccupied orbital energies of (BN)28 isomers. The present graph convolutional network establishes a direct mapping between the topological connection and the energetic or electronic properties of a cage-like cluster or molecule.

Voltage sensors of a Na+ channel dissociate from the pore domain and form inter-channel dimers in the resting state

Understanding voltage-gated sodium (Nav) channels is significant since they generate action potential. Nav channels consist of a pore domain (PD) and a voltage sensor domain (VSD). All resolved Nav structures in different gating states have VSDs that tightly interact with PDs; however, it is unclear whether VSDs attach to PDs during gating under physiological conditions. Here, we reconstituted three different voltage-dependent NavAb, which is cloned from Arcobacter butzleri, into a lipid membrane and observed their structural dynamics by high-speed atomic force microscopy on a sub-second timescale in the steady state. Surprisingly, VSDs dissociated from PDs in the mutant in the resting state and further dimerized to form cross-links between channels. This dimerization would occur at a realistic channel density, offering a potential explanation for the facilitation of positive cooperativity of channel activity in the rising phase of the action potential.

Benign bureaucracies? Religious affairs ministries as institutions of political control

ABSTRACT Despite calls for examining how authoritarian regimes employ state structures to prolong their rule and evidence that they regulate religion to shape the behaviour of religious elites, there has been little attention devoted to religious affairs ministries, which are key sites of interaction between religious actors and the state, and are often the primary institution through which regimes manage religion. This study identifies and describes eight core areas these ministries regulate that can be used as instruments for repression and co-optation of regime opponents, and state legitimation: prayer, appointments, education, religious advice and decisions, religious endowments, media, registration, and charity. In this analysis, we seek to bridge the gap between the literatures on religion, the Middle East, and authoritarianism by synthesizing recent research and analysing religious affairs ministries in the Middle East-North Africa (MENA) region. We argue that by fulfilling these functions, religion ministries are not benign bureaucracies but impactful institutions of political control. In highlighting key questions that remain unanswered, we outline a research agenda for continued advances towards theorizing how authoritarian regimes might make use of state resources to protect their rule.

Accurate transition state generation with an object-aware equivariant elementary reaction diffusion model.

Transition state search is key in chemistry for elucidating reaction mechanisms and exploring reaction networks. The search for accurate 3D transition state structures, however, requires numerous computationally intensive quantum chemistry calculations due to the complexity of potential energy surfaces. Here we developed an object-aware SE(3) equivariant diffusion model that satisfies all physical symmetries and constraints for generating sets of structures-reactant, transition state and product-in an elementary reaction. Provided reactant and product, this model generates a transition state structure in seconds instead of hours, which is typically required when performing quantum-chemistry-based optimizations. The generated transition state structures achieve a median of 0.08 Å root mean square deviation compared to the true transition state. With a confidence scoring model for uncertainty quantification, we approach an accuracy required for reaction barrier estimation (2.6 kcal mol-1) by only performing quantum chemistry-based optimizations on 14% of the most challenging reactions. We envision usefulness for our approach in constructing large reaction networks with unknown mechanisms.

Soft power in Turkey's foreign policy in Europe

Since the mid-2000s, under the rule of the Justice and Development Party, Turkey has included the concept of soft power in its foreign policy agenda. Based on the position of Turkish officials, the practical implementation of soft power is based on public diplomacy, with its “infrastructure” being created at the same time by establishing new or transforming existing state structures in partnership with non-governmental organisations. Ankara has developed the main directions for public diplomacy to be based on interaction with its Turkish people living abroad and closely related Turkic peoples, the preservation and revival of the cultural, historical and material heritage of the Ottoman Empire, and the support of the Muslim community in different parts of the world. In addition, in recent years, relatively new directions have emerged, such as struggling against FETÖ and extension of coverage of the Turkish Radio and Television Corporation. One of the regions where the Turkish public diplomacy was implemented was Europe. The latter was divided into three sub-regions: the Balkans, Eastern Europe, and Western Europe. The Balkan countries were traditionally considered important regions for Ankara with the core goal to break old historical and cultural prejudices about its image and to form a positive one in the eyes of the public. With regard to Eastern Europe, Turkey, skillfully using the Ottoman heritage and/or the Turkic factor, exerts influence in Hungary, Moldova, and Ukraine. And the activity in Western Europe is more based on interacting with the Turkish diaspora. Therefore, the aim of the study is to reveal the main directions of Turkey's public diplomacy in the context of the implementation of the policy of soft power in Europe.

PROSPECTS OF APPLICATION OF THE EXPERIENCE OF INSTITUTIONALIZATION OF LOBBYING IN THE USA IN THE DOMESTIC SYSTEM OF PUBLIC ADMINISTRATION

Based on domestic and foreign sources, the article analyzes the experience of the USA regarding the institutionalization of lobbying activities and makes a generalization about the possibility of its use in the domestic system of public administration. It is shown that the goals of the institutionalization of lobbying activities are to ensure the rights of citizens to participate in the adoption and implementation of management decisions and the activities of government bodies in general. It is substantiated that the development of lobbying contributes to the effectiveness of public control in interactions between state structures and interest groups, which is a mechanism for minimizing corruption. It is noted that the USA is a kind of standard for the institutionalization of lobbying in the system of public administration, since it was here that it was started in its modern form and it is here that legislation on lobbying has the longest history and, accordingly, detailed regulation of the object of regulation. For domestic legislators, the systematic development of the conceptual apparatus in the field of lobbying activity regulation, which includes the definition of 16 terms, is primarily important in this law. The law regulates not only the relations between lobbyists and legislators, but lobbying activities in the sphere of executive power. US lobbying legislation also defines the characteristics of lobbying activity as professional, and also has a perfect procedure for registration and submission of quarterly reports of lobbyists and a system of penalties for violations of lobbying legislation. At the same time, it is shown that the American system of lobbying regulation was not very effective in combating political corruption in the US legislative system. It was concluded that the American legislation on the regulation of lobbying can be taken as a basis for the development of the most important component of domestic legislation related to the definition of the subject and lobbying activity, as well as the concept of this activity itself. And this is the main prerequisite for successful public-management regulation of the functioning of this institute in a standardized form, which is an urgent task for the development of the domestic public management system.

Negotiating Social Protection and Care: A Study of First-Generation Older Turkish Community in London

Access to social protection in old age is crucial and yet contingent upon negotiations between the social structures of the welfare states and the personal networks within which individuals are embedded. International migration, changing family dynamics, and the transformation of care and other welfare policies in the global North make it challenging for older migrants to negotiate social protection. Drawing on 45 semi-structured interviews with first-generation older Turkish migrants in London and 13 semi-structured interviews with professional service providers for the community, the paper aims to investigate the assemblages of formal and informal social protection in the lives of older migrants. Findings indicate the complexity in accessing informal social protection and the navigation of formal care support in the UK for first generation older Turkish migrants and the contingency of access to formal care services on informal support networks for participants. It has been demonstrated that built infrastructure and policies aimed at older adults have great influence on assemblages of care, highlighting the need for more age-friendly and integrated policies to facilitate access to social protection for diverse groups of older adults.

Oxidation of the 1‐naphthyl radical C10H7• with oxygen: Thermochemistry, kinetics, and possible reaction pathways

AbstractThe reaction of the 1‐naphthyl radical C10H7• (A2•) with molecular (3O2) and atomic oxygen, as part of the oxidation reactions of naphthalene, is examined using ab‐initio and DFT quantum chemistry calculations. The study focuses on pathways that produce the intermediate final products CO, phenyl and C2H2, which may constitute a repetitive reaction sequence for the successive diminution of six‐membered rings also in larger polycyclic aromatic hydrocarbons. The primary attack of 3O2 on the 1‐naphthyl radical leads to a peroxy radical C10H7OO• (A2OO•), which undergoes further propagation and/or chain branching reactions. The thermochemistry of intermediates and transition state structures is investigated as well as the identification of all plausible reaction pathways for the A2• + O2 / A2• + O systems. Structures and enthalpies of formation for the involved species are reported along with transition state barriers and reaction pathways. Standard enthalpies of formation are calculated using ab initio (CBS‐QB3) and DFT calculations (B3LYP, M06, APFD). The reaction of A2• with 3O2 opens six main consecutive reaction channels with new ones not currently considered in oxidation mechanisms. The reaction paths comprise important exothermic chain branching reactions and the formation of unsaturated oxygenated hydrocarbon intermediates. The primary attack of 3O2 at the A2• radical has a well depth of some 50 kcal mol−1 while the six consecutive channels exhibit energy barriers below the energy of the A2• radical. The kinetic parameters of each path are determined using chemical activation analysis based on the canonical transition state theory calculations. The investigated reactions could serve as part of a comprehensive mechanism for the oxidation of naphthalene. The principal result from this study is that the consecutive reactions of the A2• radical, viz. the channels conducting to a phenyl radical C6H5•, CO2, CO (which oxidized to CO2) and C2H2 are by orders of magnitude faster than the activation of naphthalene by oxygen (A2 + O2 → A2• + HO2).

Elucidation of the structural basis for ligand binding and translocation in conserved insect odorant receptor co-receptors

In numerous insects, the olfactory receptor family forms a unique class of heteromeric cation channels. Recent progress in resolving the odorant receptor structures offers unprecedented opportunities for deciphering their molecular mechanisms of ligand recognition. Unexpectedly, these structures in apo or ligand-bound states did not reveal the pathway taken by the ligands between the extracellular space and the deep internal cavities. By combining molecular modeling with electrophysiological recordings, we identified amino acids involved in the dynamic entry pathway and the binding of VUAA1 to Drosophila melanogaster’s odorant receptor co-receptor (Orco). Our results provide evidence for the exact location of the agonist binding site and a detailed and original mechanism of ligand translocation controlled by a network of conserved residues. These findings would explain the particularly high selectivity of Orcos for their ligands.

Elucidating structural variability in p53 conformers using combinatorial refinement strategies and molecular dynamics

ABSTRACT Low molecular weight proteins and protein assemblies can now be investigated using cryo-electron microscopy (EM) as a complement to traditional structural biology techniques. It is important, however, to not lose sight of the dynamic information inherent in macromolecules that give rise to their exquisite functionality. As computational methods continue to advance the field of biomedical imaging, so must strategies to resolve the minute details of disease-related entities. Here, we employed combinatorial modeling approaches to assess flexible properties among low molecular weight proteins (~100 kDa or less). Through a blend of rigid body refinement and simulated annealing, we determined new hidden conformations for wild type p53 monomer and dimer forms. Structures for both states converged to yield new conformers, each revealing good stereochemistry and dynamic information about the protein. Based on these insights, we identified fluid parts of p53 that complement the stable central core of the protein responsible for engaging DNA. Molecular dynamics simulations corroborated the modeling results and helped pinpoint the more flexible residues in wild type p53. Overall, the new computational methods may be used to shed light on other small protein features in a vast ensemble of structural data that cannot be easily delineated by other algorithms.

Classification of administrative acts

The administrative act, a legal institution of rare preeminence in any legal system of the democratic world, represents a symbol of the structured activity of administration. It involves the manner of transposing the imperative, unequivocal, and unidirectional will of superordinate state structures into objective reality. This will enters specialized legal relations and is characterized by the exercise of public power.
 The administrative act represents, in this manner, a representative and well-defined means of imposing the will of organized state structures at every level of society by transposing informed but conceptualized will into a materialized and easily perceptible resort.
 As conceptualized in Romanian doctrine, the specific act of administrative law represents the primary and revealing form of public administration activity. It is the only legal act issued/adopted by authorities, institutions, or other public bodies that produces concrete effects concerning its recipients and beyond.
 However, this distinct type of legal act is characterized by a series of distinctive features that individualize it within the framework of internal legal relations and reveal its specific aspects, as well as the effects emanating from its content.
 Not all administrative acts have the same form, structure, effects, characteristics, purposes, and certainly, not all have the same impact on the individualized legal order at the level of society.
 Therefore, in specialized doctrine followed by the practice of national courts, a series of distinct criteria have been imposed for crystallizing distinct typologies of administrative acts.

Macrocyclization-induce emission enhancement characteristics of benzothiadiazole-based macrocycles: A theoretical study

We present a comprehensive investigation into the molecular structures in the ground state and the first singlet excited state, intramolecular and intermolecular interactions, as well as the emission properties of monomers, dimers and macrocycles using density functional theory and time-dependent density functional theory. Our study aims to unveil the macrocyclization-induce emission enhancement principle for benzothiadiazole-based macrocycles. Compared with the typical π-π stacking of monomers, macrocycles exhibit the tighter stacking arrangement due to the presence of strong intramolecular and intermolecular hydrogen bond interactions and the rigid triangular geometry of macrocycle, which is conducive to the enhancement of emissions efficiency. The calculated oscillator strength of macrocycles in the first excited state is much higher than that of dimers composed of two monomers. Meanwhile, the large transition electric dipole moment is beneficial for enhancing the emission efficiency. Additionally, our study reveals an emission wavelength red-shift for macrocycles after “CH”/N substitution in the acceptor moiety, and the transition mode from LUMO to HOMO can be mainly attributed to intramolecular charge transition characters from acceptor to donor fragments. Our theoretical study might provide valuable insights for the design of innovative luminescent macrocycles with high emission efficiency.

Molecular dynamics simulations suggest novel allosteric modes in the Hsp70 chaperone protein

The Hsp70 chaperone protein system is an essential component of the protein folding and homeostasis machinery in E.Coli. Hsp70 is a three domain, 70 kDa protein which functions as an allosteric system cycling between an ADP-bound state where the three domains are loosely coupled via a flexible interdomain linker and an ATP-bound state where they are tightly coupled into a single entity. The structure-function model of this protein proposes an allosteric connection between the 45 kDa Nucleotide Binding Domain (NBD) and the 25 kDa Substrate Binding Domain (SBD) and Lid Domain which operates through the inter NBD-SBD linker. X-Ray crystallography and NMR spectroscopy have provided structures of the end states of the functional cycle of this protein, bound to ADP and ATP. We have used MD simulations to study the transitions between these end states and allosteric communication in this system. Our results largely validate the experimentally derived allosteric model of function, but shed additional light on the flow of allosteric information in the SBD + Lid. Specifically, we find that the Lid domain has a double-hinged structure with the potential for greater conformational flexibility than was hitherto expected.Communicated by Ramaswamy H. Sarma