Stages Of Evolution Research Articles

Evolutionary Stages of Feminine Consciousness in "Makhota"

Evolutionary Stages of Feminine Consciousness in "Makhota"

Introduction of Western Medicines into China and Improvement on Decoctions during Modern China

Abstract: During the late Qing dynasty (1840 A.D.-1912 A.D.), a large quantity of Western medicines entered China, which continuously impacted the traditional Chinese medicine (TCM) market and revealed the shortcomings of Chinese medicines. Some personages in the TCM community followed the trend of learning from the West, and attempted to reform TCM, with the improvement on decoction becoming an important aspect of this effort. Through debates and trials, the improvement on decoction underwent three stages of conceptual evolution: “taking Chinese medicines as the foundation and referring to the dosage forms of Western medicines”, “introducing Western techniques to serve the preparation of decoctions” and “integrating the theories of TCM and Western medicine to improve decoctions”. The study on the improvement on decoction highlights the effective complementarity between modern TCM and Western medicine in the field of pharmacy, and provides valuable experience and support for the reevaluation of the value of TCM in contemporary society.

GA-NIFS: Early-stage feedback in a heavily obscured active galactic nucleus at z = 4.76

Dust-obscured galaxies are thought to represent an early evolutionary phase of massive galaxies in which the active galactic nucleus (AGN) is still deeply buried in significant amounts of dusty material and its emission is strongly suppressed. The unprecedented sensitivity of the James Webb Space Telescope (JWST) enabled us for the first time to detect the rest-frame optical emission of heavily obscured AGNs and unveil the properties of the hidden accreting super-massive black holes (BHs). In this work, we present the JWST/NIRSpec integral field spectroscopy (IFS) data of ALESS073.1, a massive (log(M⋆/M⊙) = 10.98) dusty, star-forming galaxy at z = 4.755 hosting an AGN at its center. The detection of a very broad (> 9000 km s−1) Hα emission associated with the broad line region (BLR) confirms the presence of a BH (log(MBH/M⊙) > 8.7) accreting at less than 18% of its Eddington limit. The identification of the BLR classifies the target as a type 1 AGN despite the observed high column density of NH ∼ 1024 cm−2. The rest-frame optical emission lines also reveal a fast (∼1700 km s−1) ionized gas outflow marginally resolved in the galaxy center. The high sensitivity of NIRSpec allowed us to perform the kinematic analysis of the narrow Hα component, which indicates that the warm ionized gas velocity field is consistent with disk rotation. Interestingly, we find that in the innermost nuclear regions (< 1.5 kpc), the intrinsic velocity dispersion of the disk reaches ∼150 km s−1, which is ∼2 − 3 times higher than the velocity dispersion inferred from the [C II] 158 μm line tracing mostly cold gas. Since at large radii the velocity dispersion of the warm and cold gas are comparable, we conclude that the outflows are injecting turbulence in the warm ionized gas in the central region, but they are not sufficiently powerful to disrupt the dense gas and quench star formation. These findings support the scenario that dust-obscured galaxies represent the evolutionary stage preceding the unobscured quasar when all gas and dust are removed from the host.

Anaerobic fungi in the tortoise alimentary tract illuminate early stages of host-fungal symbiosis and Neocallimastigomycota evolution

Anaerobic gut fungi (AGF, Neocallimastigomycota) reside in the alimentary tract of herbivores. While their presence in mammals is well documented, evidence for their occurrence in non-mammalian hosts is currently sparse. Culture-independent surveys of AGF in tortoises identified a unique community, with three novel deep-branching genera representing >90% of sequences in most samples. Representatives of all genera were successfully isolated under strict anaerobic conditions. Transcriptomics-enabled phylogenomic and molecular dating analyses indicated an ancient, deep-branching position in the AGF tree for these genera, with an evolutionary divergence time estimate of 104-112 million years ago (Mya). Such estimates push the establishment of animal-Neocallimastigomycota symbiosis from the late to the early Cretaceous. Further, tortoise-associated isolates (T-AGF) exhibited limited capacity for plant polysaccharides metabolism and lacked genes encoding several carbohydrate-active enzyme (CAZyme) families. Finally, we demonstrate that the observed curtailed degradation capacities and reduced CAZyme repertoire is driven by the paucity of horizontal gene transfer (HGT) in T-AGF genomes, compared to their mammalian counterparts. This reduced capacity was reflected in an altered cellulosomal production capacity in T-AGF. Our findings provide insights into the phylogenetic diversity, ecological distribution, evolutionary history, evolution of fungal-host nutritional symbiosis, and dynamics of genes acquisition in Neocallimastigomycota.

Landscape evolution of the NW Himalayan rivers during the late Quaternary and their non-contemporaneity to the Harappan Civilization

A large perennial river system, the Ghaggar-Hakra, fed by the palaeo-Sutlej and palaeo-Yamuna Rivers from the west and east respectively, has been argued to have sustained the Bronze Age Harappan urban settlements (∼4.6–3.9 ka BP) in NW Himalayan foreland in India and Pakistan. However, it has been demonstrated by previous workers that palaeo-Sutlej was already defunct in this region by ∼8 ka, much before the peak of urbanism. In contrast, the evolutionary history of palaeo-Yamuna, a major feeder to the Ghaggar-Hakra River system, remains weakly documented and enigmatic. This knowledge gap limits our understanding of the influence of regional-scale hydrological conditions on the growth and eventual demise of the Harappan Civilization. Here, we present the high-resolution chronostratigraphic records based on 47 optically stimulated luminescence (OSL) ages from 6 cores (∼50 m deep) retrieved across the palaeochannels of the Yamuna. We document multi-storied sand bodies deposited by a mobile channel belt in a large alluvial fan system characterized by four possible stages of drainage reorganization and landscape evolution during the late Quaternary. It has also been inferred that a major eastward shift of the palaeo-Yamuna River occurred at ∼18 ka, predating both the westward shift of the Sutlej at ∼8 ka and the decline of the Harappan Civilization at ∼3.9 ka BP. Our chronostratigraphic data helps to understand the drainage reorganization of the Himalayan river systems during the late Quaternary in the NW Himalayan foreland and reaffirms the non-contemporaneity of the Harappan Civilization with a large river system.

Geochemical Characterization and Genetic Analysis of Rocks of the Dianzhong Formation in Geda Township Area,South-Central Tibet

The study on the genetic and geochemical characteristics of volcanic rocks of the Cretaceous-Paleogene Dienzhong Formation in central and southern Tibet is of great significance for the establishment of magma evolution sequence in the working area. The volcanic rocks of Dianzhong Formation in Geda area can be roughly divided into dite, andesite and rhyolite. Zircon U-Pb isotopic dating results show that the weighted average age of Pb206/U238 of oligopleural rhyolite and Andesite samples is 65. 5±2. 3Ma (MSWD=2. 0) and 68. 7±3. 1Ma (MSWD=2. 0) , respectively. This indicates that the middle acid volcanic rocks of Dianzhong Formation erupted from Late Cretaceous to Paleocene. Geochemical analysis of rocks shows that the acidic volcanic rocks in Dianzhong Formation are a set of meta-aluminous-peraluminouscalc- alkaline andesite-dacite-rhyolite rock assemblages. Large ion lithophile elements and light rare earth elements are enriched, and high field strength elements are deficient. In the process of magmatic evolution, there is a large amount of crystallization of plagiase or a large amount of refractory residue of plagiase, and there are a lot of continental crust materials in the volcanic rocks of the Dian Zhong Formation. Comprehensive analysis shows that the volcanic rocks of Dianzhong Formation have the chemical characteristics of continental arc volcanic rocks, and may be magmatic products in the transition stage from subduction mechanism to continental collision mechanism. Zircon U-Pb dating, in situ Lu-Hf isotope and whole rock principal and trace analysis were carried out on the acidic volcanic rocks in Dianzhong Formation, Geda Area. The aim is to study the geochemical characteristics and evolutionary origin of rocks in this area. It provides the geochemical data support for the discussion on the division of tectonic evolution stages in the study area since Jurassic.

Agents and mechanisms of political domination, or how a “winning coalition” rules

Considering the relationship between state and society as the main area of thematic division of power “over” and “for”, the author shows the grounds that reveal that political domination is a certain form and stage of the historical evolution of political power, reflecting (in contrast to the democratic version of its development) the gradual usurpation by the ruling circles of their institutional (general civil) powers, truncation of citizens' rights and reduction of opportunities for their participation in government decision-making. In this context, the author, considering internal elite conflicts as the main source of such an evolution of power relations, draws attention to the so-called “winning” coalition of the ruling class, which concentrated in its hands the main instruments of control over government decision-making and the distribution of key public goods and resources. At the same time, a complex set of functions and tasks for developing public policy goals, identifying privileged groups and maintaining social stability predetermines the identification of internal groupings of this elite stratum, allowing it to manage and construct social relations in accordance with the stated political course and its hidden intentions. In this context, the article identifies seven main segments of this ruling group, performing various tasks in the field of competition with rival groups of the ruling class and in the field of relations with society and structures of the civil sector. In this regard, the corresponding contradictions between individual segments of the “winning” coalition are identified, as well as the technologies and mechanisms characteristic of these elite groups that contribute to the fulfillment of their role and support the format of political dominance.

On the Legal Concept of Metaverses

The paper offers a detailed model of the legal concept of metaverses. The scientific impetus for this discourse was the foreign practice of developing metaverses, which have become national priorities for a number of states. According to the author’s position, the metaverse is a direction (area) of digital technology development for the formation of a digital space for various types of social and public activities with imitation of the personal presence of the user. The components of the metaverse interact with each other, are in dynamic balance, creating a digital industry. The industry of the metaverse is at a stage of continuous evolution and development. It consists of five main systems, including a technological system, a system of standards, a system of digital products, a service and warranty system. Based on this conclusion, the author systematizes approaches to the development of legal regulation of relations in the metaverses in order to consolidate the country’s technological leadershipin this promising direction. As the metaverse evolves, it may become a promising space for the circulation of digital data and the implementation of other ambitious projects of the digital economy. However, the emergence of new threats and risks in artificial virtual worlds requires the development and adoption of a concept of legal understanding of metaverses, as well as the advanced study of Russian legislation guaranteeing human rights and freedoms in metaverses.

Deciphering interfacial properties and intermediaries via probing lattice deformations: Growth solution chemistry for mixed/pure phase metal oxide/perovskites

pH of the growth solution of nanomaterials has been employed as a controlling agent in the morphology and synthesis of various metal oxides. The key points in the reconciliation with the thus formed nanostructures inevitably revolve around the impact of H+ and OH- ions on the oxide phases formed during various stages of the phase evolution. Here, a careful elucidation of acidic and alkaline conditions that influence the evolution of the perovskite of Strontium titanate (STO) has been done taking care to avoid the influence of other reagents by avoiding their use altogether. Multiple incremental steps of the growth solution’s pH were the only variable, allowing a direct correlation of the alkalinity with the several interesting materializations of the perovskite composition. The fundamental aspects on the development of the different phases such as the lattice strains due to expansions and contractions of the lattice, integration of shallow level defects as a result of the growth conditions, visualizations of the interfaces between multiple phases which were always present when the growth conditions were not completely alkaline have been revealed through different structural and morphological characterizations. The catalytic property of the samples prepared at different pH levels investigated using UV light exhibited markedly different photocatalytic activity toward Rhodamine B (RhB). We propose that the tuned phase compositions and morphologies are the reason for the observed variations. The different compositions of intermediaries are interesting candidates for different applications that are concerned with defect and interface engineering, and the data presented herewith reveal precise conditions for achieving each of these interesting compositions as well as realizing pristine STO without the typical presence of secondary phases such as carbonates.

FORMATION OF TWO-PHASE ZONES DURING INTERNAL OXIDATION OF BINARY ALLOYS

In the paper the formation patterns of two-phase regions during the internal oxidation process in low-alloyed binary alloys is studied. The goal of the research is the creation of a mathematical model for the internal oxidation process, which is to describe the kinetics of formation and evolution of internal oxidation zones, the study of the patterns governing the two-phase zone formation during internal oxidation, the study of the space-time dynamics of the structural parameters of the two-phase area, namely the number of the oxide particles, their average radius and the phase volume, at different stages of the internal oxidation zone evolution. The two-phase process of diffusion saturation with light components applied to binary alloys is analyzed theoretically. The conditions for formation of disperse oxide particles are formulated. A mathematical model for the formation of two-phase areas as well as the numerical solution for the system of equations describing the kinet- ics of formation and evolution of the two-phase area for different values of the process parameters is proposed. The modeling and experiments demonstrate that the distribution of structural parameters of the two-phase area has complex, non-monotonous nature. The influence of the main parameters of the process on the kinetics of the formation of the two-phase area in a lightly-alloyed binary nickel-based alloy plate is also determined. All stages of the plate’s oxidation process are studied – from the inception of the particles to the coalescence of second-stage particles. The results of the work could be used for developing new technologies in thermal and chemical-thermal treatments for disperse strengthening of materials using internal oxidation, as a physical basis for seeking ways of providing certain distributions of the internal oxidation zone structural parameters, which are required to give the al- loy a necessary set of physical and mechanical properties. The comparison of the modeling and experimental results shows a good correlation, which allows them to be recommended for developing new technologies in disperse strengthening, new heat-resistant steels, disperse-strengthened magnetic materials and electrotechnical alloys.

Magnetic activity of radio stars based on TESS and LAMOST surveys

Aims. Stellar flares serve as crucial indicators stellar magnetic activity. Radio emissions were detectable across all stages of stellar evolution. We define radio stars in our paper as stars with radio continuum emission in the frequency region of about 1.4 - 375 GHz from the catalog published by Wendker (1995, 2015). We also include detected radio stars from the LOFAR Two-metre Sky Survey (LoTSS) and the Australian Square Kilometre Array Pathfinder (ASKAP), and VLA Sky Survey (VLASS) surveys, and present comparative discussions. We used the light curves from the Transiting Exoplanet Survey Satellite (TESS) survey coupled with low- and medium-resolution spectra from the Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST) survey to investigate the magnetic activity and statistical properties of radio stars. Methods. We cross-matched the radio stars from the radio star catalog and the LoTSS, ASKAP, and VLASS surveys with the TESS survey. For the stars from the radio star catalog, we obtained a matched sample of 1,537 stars (hereafter Sample 1) and downloaded their TESS light curves, which include 4,001 light curves at a 120s cadence. For the stars from the LoTSS, ASKAP, and VLASS surveys, we obtain a matched sample of 98 stars (hereafter Sample 2) and downloaded their 120s TESS light curves, totaling 260. Subsequently, we employed repeated fitting techniques to distinguish stellar background light curves from flare events. For Sample 1, we successfully identify 12,155 flare events occurring on 856 stars. For Sample 2, we identify a total of 3,992 flare events on 86 stars. Furthermore, by cross-referencing our samples with the Gaia survey, the TESS Input Catalog, and the LAMOST survey, we obtain additional stellar parameters, facilitating the determination of relationships between stellar and flare parameters. Results. For stars in Sample 1, within the 12,155 flare events observed on the 856 flare-active radio stars, a majority of more than 97$<!PCT!>$ have durations of less than 2 hours, while for stars in Sample 2, all 3,992 flare events have a duration of less than 2 hours. We calculated the flare occurrence percentage for each flare-active radio star, observing a decrease as effective temperature increased for both Sample 1 and Sample 2. We derived values of the power-law index (for Sample 1, the alpha value is approximately 1.50pm 0.11 for single stars and 1.38pm 0.09 for binary stars, for Sample 2, the alpha value is about 1.47pm 0.11 for single stars and 1.42pm 0.09 for binary stars) for the cumulative flare frequency distribution. In both Sample 1 and Sample 2, stars with lower effective temperatures tend to exhibit increased activity. Using LAMOST spectra and the Gaia DR3 chromospheric activity index, we note that the Halpha equivalent width and Gaia Ca II IRT activity index of flare-active radio stars is significantly larger than that of nonflaring stars. An intriguing finding is our potential identification of a coronal rain candidate through asymmetry observed in the Halpha line.

Phase-resolved XMM-Newton observations of the massive post-RLOF system HD 149404

We investigated the X-ray emission of HD\,149404, a 9.81-day period O-star binary in a post-Roche lobe overflow evolutionary stage. X-ray emission of O-star binaries consists of the intrinsic emission of the individual O stars and a putative additional component arising from the wind-wind interaction. Phase-locked variations in the X-ray spectra can be used to probe the properties of the stellar winds of such systems. XMM-Newton observations of HD\,149404 collected at two conjunction phases and a quadrature phase were analysed. X-ray spectra were extracted and flux variations as a function of orbital phase were inferred. The flux ratios were analysed with models considering various origins for the X-ray emission. The highest and lowest X-ray fluxes are observed at conjunction phases respectively with the primary and secondary star in front. The flux variations are nearly grey with only marginal energy dependence. None of the models accounting for photoelectric absorption by homogeneous stellar winds perfectly reproduces the observed variations. Whilst the overall X-ray luminosity is consistent with a pure intrinsic emission, the best formal agreement with the observed variations is obtained with a model assuming pure wind-wind collision X-ray emission. The lack of significant energy-dependence of the opacity most likely hints at the presence of optically thick clumps in the winds of HD\,149404.

MorphVAE: Advancing Morphological Design of Voxel-Based Soft Robots with Variational Autoencoders

Soft robot design is an intricate field with unique challenges due to its complex and vast search space. In the past literature, evolutionary computation algorithms, including novel probabilistic generative models (PGMs), have shown potential in this realm. However, these methods are sample inefficient and predominantly focus on rigid robots in locomotion tasks, which limit their performance and application in robot design automation. In this work, we propose MorphVAE, an innovative PGM that incorporates a multi-task training scheme and a meticulously crafted sampling technique termed ``continuous natural selection'', aimed at bolstering sample efficiency. This method empowers us to gain insights from assessed samples across diverse tasks and temporal evolutionary stages, while simultaneously maintaining a delicate balance between optimization efficiency and biodiversity. Through extensive experiments in various locomotion and manipulation tasks, we substantiate the efficiency of MorphVAE in generating high-performing and diverse designs, surpassing the performance of competitive baselines.

Lacustrine deposition in response to the middle eocene climate evolution and tectonic activities, Bohai Bay Basin, China

The lower unit of the third member of the Eocene Shahejie Formation (Es3L, 42.5 to ∼40Ma) is one of the most important petroleum exploration targets in the Bohai Bay Basin. Lacustrine deposits are widely distributed in the Es3Lunit of the Shulu Sag and display strong vertical and horizontal variations in lithofacies and geochemical characteristics. Based on core data, thin-section petrography, stable δ13C isotope analyses, and organic geochemistry data, eight lithofacies and six evolutionary lake stages are identified. Combining the results of the astronomical time scale (ATS) and the radiometric age, the correlation between the evolutionary lake stages of Es3Land the Eocene global climate curve was illustrated. Tectonic movement caused the basin to subside and resulted in shallow lake formation (Stage 1) during the cooling phase prior to the Middle Eocene Climatic Optimum (MECO); then, the lake level began to rise (Stage 2) with an increasingly warm and humid climate. Stage 3 corresponds to the peak of the MECO and was influenced by the warm and humid climate during the thermal maximum period, forming a deep, freshwater lake. This stage was followed by highly turbulent lake conditions (Stage 4), which were influenced by both the cooling climate and intensive tectonic activity. Subsequently, a stable lake (Stage 5) was formed, which was mainly controlled by the slightly warming climate since the tectonic movement was relatively stable, followed by another stage of turbulent lake conditions (Stage 6). The results show that the global mid-Eocene climate played an important role on the relative lake level changes and TOC enrichment. The MECO led to a rise in the relative sea-level and the obviously rise in relative lake-level in the Bohai Bay Basin. The evolution of the depositional settings was controlled by both global climate and tectonic activities and accounted for the deposition and distribution of lithofacies with distinctly different characteristics and origins.

Nature of the diffuse emission sources in the H i supershell in the galaxy IC 1613

ABSTRACT We present a study of the nearby low-metallicity dwarf galaxy IC 1613, focusing on the search for massive stars and related feedback processes, as well as for faint supernova remnants (SNR) in late stages of evolution. We obtained the deepest images of IC 1613 in the narrow-band H α, He ii and [S ii] emission lines and new long-slit spectroscopy observations using several facilities (6-m BTA, 2.5m SAI MSU, and 150RTT telescopes), in combination with the multiwavelength archival data from MUSE/VLT, VLA, XMM–Newton, and Swift/XRT. Our deep narrow-band photometry identifies several faint shells in the galaxy, and we further investigate their physical characteristics with the new long-slit spectroscopy observations and the archival multiwavelength data. Based on energy balance calculations and assumptions about their possible nature, we propose that one of the shells is a possible remnant of a supernova explosion. We study five out of eight Wolf–Rayet (WR) star candidates previously published for this galaxy using the He ii emission line mapping, MUSE/VLT archival spectra, and new long-slit spectra. Our analysis discards the considered WR candidates and finds no new ones. We found P Cyg profiles in H α line in two stars, which we classify as Luminous Blue Variable (LBV) star candidates. Overall, the galaxy IC 1613 may have a lower rate of WR star formation than previously suggested.

Partial mycoheterotrophy in the leafless orchid Eulophia zollingeri specialized on wood-decaying fungi.

Although the absence of normal leaves is often considered a sign of full heterotrophy, some plants remain at least partially autotrophic despite their leafless habit. Leafless orchids with green stems and capsules probably represent a late evolutionary stage toward full mycoheterotrophy and serve as valuable models for understanding the pathways leading to this nutritional strategy. In this study, based on molecular barcoding and isotopic analysis, we explored the physiological ecology of the leafless orchid Eulophia zollingeri, which displays green coloration, particularly during its fruiting phase. Although previous studies hadshown thatE. zollingeri, in its adult stage, is associated with Psathyrellaceae fungi and exhibits high 13C isotope signatures similar to fully mycoheterotrophic orchids, it remained uncertain whether this symbiotic relationship is consistent throughout the orchid's entire life cycle and whether the orchid relies exclusively on mycoheterotrophy for its nutrition during the fruiting season. Our study has demonstratedthat E. zollingeri maintains a specialized symbiotic relationship with Psathyrellaceae fungi throughout all life stages. However, isotopic analysis and chlorophyll data have shown that the orchid also engages in photosynthesis to meet its carbon needs, particularly during the fruiting stage. This research constitutes the first discovery of partial mycoheterotrophy in leafless orchids associated with saprotrophic non-rhizoctonia fungi.

Review on " Hepatocellular carcinoma"

Hepatocellular carcinoma is a problem for the world's Hepatocellular carcinoma, metabolic syndrome, stage of liver cancer local disease, liver cirrhosis, treatment. syndrome or diabetes mellitus is increasingly common in the west. Furthermore, HCC with non-alcoholic steatohepatitis-associated molecular pathogensis is distinct. A quarter of all HCCs have potentially treatable mutations that have not yet been applied in clinical settings. When clinical deterioration occurs, HCC is frequently detected, and survival is then assessed as a risk factor. utilizing alfa-fetoprotein (AFP), a serum maker, frequently in conjunction with ultrasound. The accuracy of monitoring is currently being improved by testing a number of other serologic indicators. More advanced imaging techniques, such CT scan and MRI, which have multiphasic contract enhancing capabilities, are frequently needed for the diagnosis of HCC. When serum AFP levels are significantly increased, which happens in less than half of cases at the time of diagnosis, it can be useful to use serum AFP alone. When the diagnosis of HCC is still undetermined, a liver biopsy might be used to confirm the diagnosis. The present requirement for molecular information, which necessitates tissue or liquid biopsies, challenges diagnosis based on non-invasive criteria. The management of patients with advanced HCC has been altered by recent significant developments. Checkpoint inhibitors, tyrosine, and even combinations of two immunotherapy treatments are among the combination medicines that new trials are investigating. The results of these trials are anticipated to alter the HCC management environment at all evolutionary stages.

Abstract 1622: Characterization of the phylogenetic relationships in bilateral breast angiosarcoma

Abstract Background: Breast angiosarcoma represents around 20% of all angiosarcomas, and often spread to lung, liver, and bones. Driver mutations of angiosarcoma, specifically PTPRB, and PLCG1, are well-known as reported previously. Notably, contralateral breast is another metastatic site of the breast angiosarcoma, where an unusual location for other soft tissue sarcomas. However, it remains uncertain whether the contralateral disease originates from the primary tumor or emerges independently. Here, we present a 75-year-old woman with bilateral breast angiosarcomas to investigate the genetic relationship between the tumors. Methods: Irregular and heterogeneous masses were synchronously detected through breast sonography. Whole genome sequencing (WGS) was conducted at 60X coverage using bilateral total mastectomies, along with a matched normal blood sample (n=1). Single nucleotide variants (SNVs) and small indels were identified using Strelka2 and Varscan2. Subsequent filtering was performed using custom Python scripts to establish high-confidence variants. A phylogenetic tree was then reconstructed based on SNVs to trace clonal evolution and distinguish between de novo primary and metastasis. Following this, COSMIC mutational signatures were analyzed to characterize the genomic profile. Results: For the left breast angiosarcoma, we found 1,086 SNVs and 101 indels, while the right breast angiosarcoma had 921 SNVs and 92 indels. Despite their small size (<1.0 cm) and low FNCLCC grade (G1), these two cancers shared 600 SNVs and 135 indels as “truncal events”, indicating that one side metastasized from the other. According to the phylogenetic tree and the molecular clock of the two angiosarcomas, we estimated that the metastasis occurred during the middle stages of cancer evolution. Additionally, we identified 7 nonsynonymous SNVs (ADAMTS7, RPS15, CYP2A6, OR1S2, ARHGAP23, GNAQ, UGT2B7), five of which were shared. Remarkably, the GNAQ driver mutation exhibited a mutation at codon 209, a hotspot mutation not previously reported in breast angiosarcoma. The dominant Cosmic mutational signatures were SBS1/5 and ID1/2, all of which were associated with aging. Conclusion: Our findings demonstrate that bilateral breast angiosarcomas metastasized from one side to the other, as evidenced by the WGS data. This study underscores that the capability of WGS to distinguish between oligometastatic disease and multiple primary cancers, which has implications for predicting patients’ prognosis. Citation Format: Minju Kim, Jeonghwan Youk, Soyeon Kim, Miso Kim, Bhumsuk Keam, Tae Min Kim, Dong-Wan Kim, Myung Geun Song, Jiwon Koh, Jeong Mo Bae, Hyeong-Gon Moon, Seock-Ah Im. Characterization of the phylogenetic relationships in bilateral breast angiosarcoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 1622.

In Situ Helium Isotope Microimaging of Meteorites.

Isotope imaging is commonly used to investigate the localization of trace elements and their isotopes. In situ noble gas analysis of meteorites revealed the distribution of primordial noble gases that were trapped in the building blocks of asteroids and planets during the early stage of the solar system evolution. Solar wind noble gases are among the primordial gases present in meteorites and were trapped through exposure to solar wind. Micrometer-resolution in situ noble gas analysis has not been achieved due to the lack of sensitivity and spatial resolution. The microscale imaging technique is crucial for identifying the carrier phase of the solar wind noble gases. We have developed 4He isotope imaging utilizing secondary neutral mass spectrometry with strong field postionization. This technique achieved a lateral resolution of 2 μm and a 4He detection limit of 2 × 1017 cm-3. This development allows for the study of a solar wind gas-rich meteorite, Northwest Africa 801 carbonaceous chondrite, with micrometer resolution. The solar wind 4He carriers are fine-grained particles and are sparsely scattered in the matrix region.

CO outflows from young stars in the NGC2023 cluster

Young early-type HAeBe stars are still embedded in the molecular clouds in which they formed. They illuminate reflection nebulae, which shape the surrounding molecular cloud and may trigger star formation. They are therefore ideal places to search for ongoing star formation activity. NGC\,2023 is illuminated by the Herbig Be star HD\,37903. It is the most massive member of a small young cluster with about 30 PMS stars, several of which are Class I objects that still heavily accrete. It might therefore be expected that they might drive molecular outflows. We examined the whole region for outflows. We analyzed previously published APEX data to search for and characterize the outflows in the NGC\,2023 region. This is the first systematic search for molecular outflows in this region. Since the outflows were mapped in several CO transitions, we can determine their properties quite well. We have discovered four molecular outflows in the vicinity of NGC\,2023, three of which are associated with Class I objects. MIR-63, a bright mid-infrared and submillimeter Class I source, is a binary with a separation of 2 and drives two bipolar outflows orthogonal to each other. The large southeast--northwest outflow excites the Herbig-Haro flow HH\,247. MIR-73, a Class I object, which is also a far-infrared source, drives a pole-on outflow. MIR-62 is a Class II object with strong infrared excess and a luminosity of 7 It is not detected in the far-infrared. The Class I sources have bolometric luminosities of about 20 or lower, that is, they are all low-mass stars. One other far-infrared source, MIR-75, may have powered an outflow in the past because it now illuminates an egg-shaped cavity. The four outflows are all powered by young stars and are located in the immediate vicinity of NGC\,2023. They are at a similar evolutionary stage, which suggests that their formation may have been triggered by the expanding region.