Specific Structure Research Articles

The biportal transorbital approach: quantitative comparison of the anterior subfrontal craniotomy, bilateral transorbital endoscopic, and microscopic approaches.

The aim of this study was to assess the surgical use and applicability of a biportal bitransorbital approach. Single-portal transorbital and combined transorbital transnasal approaches have been used in clinical practice, but no study has assessed the surgical use and applicability of a biportal bitransorbital approach. Ten cadaver specimens underwent midline anterior subfrontal (ASub), bilateral transorbital microsurgery (bTMS), and bilateral transorbital neuroendoscopic surgery (bTONES) approaches. Morphometric analyses included the length of the bilateral cranial nerves I and II, the optic tract, and A1; the area of exposure of the anterior cranial fossa floor; craniocaudal and mediolateral angles of attack (AOAs); and volume of surgical freedom (VSF; maximal available working volume for a specific surgical corridor and surgical target structure normalized to a height of 10 mm) of the bilateral paraclinoid internal carotid arteries (ICAs), bilateral terminal ICAs, and anterior communicating artery (ACoA). Analyses were conducted to determine whether the biportal approach was associated with greater instrument freedom. The bTMS and bTONES approaches provided limited access to the bilateral A1 segments and the ACoA, which were inaccessible in 30% (bTMS) and 60% (bTONES) of exposures. The average total frontal lobe area of exposure (AOE) was 1648.4 mm2 (range 1516.6-1958.8 mm2) for ASub, 1658.9 mm2 (1274.6-1988.2 mm2) for bTMS, and 1914.9 mm2 (1834.2-2014.2 mm2) for bTONES exposures, with no statistically significant superiority between any of the 3 approaches (p = 0.28). The bTMS and bTONES approaches were significantly associated with decreases of 8.7 mm3 normalized volume (p = 0.005) and 14.3 mm3 normalized volume (p < 0.001) for VSF of the right paraclinoid ICA compared with the ASub approach. No statistically significant difference in surgical freedom was noted between all 3 approaches when targeting the bilateral terminal ICA. The bTONES approach was significantly associated with a decrease of 105% in the (log) VSF of the ACoA compared with the ASub (p = 0.009). Although the biportal approach is intended to improve maneuverability within these minimally invasive approaches, these results illustrate the pertinent issue of surgical corridor crowding and the importance of surgical trajectory planning. A biportal transorbital approach provides improved visualization but does not improve surgical freedom. Furthermore, although it affords impressive anterior cranial fossa AOE, it is unsuitable for addressing midline lesions because the preserved orbital rim restricts lateral movement. Further comparative studies will elucidate whether a combined transorbital transnasal route is preferable to minimize skull base destruction and maximize instrument access.

Addressing electronic and dynamical evolution of molecules and molecular clusters: DFTB simulations of energy relaxation in polycyclic aromatic hydrocarbons.

We present a review of the capabilities of the density functional based Tight Binding (DFTB) scheme to address the electronic relaxation and dynamical evolution of molecules and molecular clusters following energy deposition via either collision or photoabsorption. The basics and extensions of DFTB for addressing these systems and in particular their electronic states and their dynamical evolution are reviewed. Applications to PAH molecules and clusters, carbonaceous systems of major interest in astrochemical/astrophysical context, are reported. A variety of processes are examined and discussed such as collisional hydrogenation, fast collisional processes and induced electronic and charge dynamics, collision-induced fragmentation, photo-induced fragmentation, relaxation in high electronic states, electronic-to-vibrational energy conversion and statistical versus non-statistical fragmentation. This review illustrates how simulations may help to unravel different relaxation mechanisms depending on various factors such as the system size, specific electronic structure or excitation conditions, in close connection with experiments.

Temperature driven transformations of glycine molecules embedded in interstellar ice.

The formation of glycine amino acid on ice grains in space raises fundamental questions about glycine chemistry in interstellar media. In this work, we studied glycine conformational space and the related tautomerization mechanisms in water media by means of QM/MM molecular dynamics simulations of four glycine conformational isomers (cc, ct, tc, and tt). Interstellar low density amorphous (LDA) ice and T = 20 K were considered as representative for a cold interstellar ice environment, while temperatures of 250 and 450 K were included to model rapid local heating in the ice. In addition to the LDA environment, water clusters with 4, 17, and 27 H2O molecules were subjected to QM/MM dynamics simulations that allowed glycine tautomerization behaviour to be evaluated in water surface-like environments. The tautomerization processes were found to be strongly dependent on the number of water molecules and specific isomer structure. All the glycine isomers mostly preserve their canonical "neutral" conformations under interstellar conditions.

What are the minimal folding seeds in proteins? Experimental and theoretical assessment of secondary structure propensities of small peptide fragments.

Certain peptide sequences, some of them as short as amino acid triplets, are significantly overpopulated in specific secondary structure motifs in folded protein structures. For example, 74% of the EAM triplet is found in α-helices, and only 3% occurs in the extended parts of proteins (typically β-sheets). In contrast, other triplets (such as VIV and IYI) appear almost exclusively in extended parts (79% and 69%, respectively). In order to determine whether such preferences are structurally encoded in a particular peptide fragment or appear only at the level of a complex protein structure, NMR, VCD, and ECD experiments were carried out on selected tripeptides: EAM (denoted as pro-'α-helical' in proteins), KAM(α), ALA(α), DIC(α), EKF(α), IYI(pro-β-sheet or more generally, pro-extended), and VIV(β), and the reference α-helical CATWEAMEKCK undecapeptide. The experimental data were in very good agreement with extensive quantum mechanical conformational sampling. Altogether, we clearly showed that the pro-helical vs. pro-extended propensities start to emerge already at the level of tripeptides and can be fully developed at longer sequences. We postulate that certain short peptide sequences can be considered minimal "folding seeds". Admittedly, the inherent secondary structure propensity can be overruled by the large intramolecular interaction energies within the folded and compact protein structures. Still, the correlation of experimental and computational data presented herein suggests that the secondary structure propensity should be considered as one of the key factors that may lead to understanding the underlying physico-chemical principles of protein structure and folding from the first principles.

Recombinant Production of Glycoengineered Mucins in HEK293-F Cells.

Recombinant mucins are attractive polymeric building blocks for new biomaterials, biolubricants, and therapeutics. Advances in glycoengineered host cell systems now enable the recombinant production of mucins with tailored O-glycan side chains, offering new opportunities to tune the functionality of mucins and investigate the biology of specific O-glycan structures. Here, we provide a protocol for the scalable production of glycoengineered mucins and mucin-like glycoproteins in suspension-adapted HEK293-F cells. The protocol includes the preparation of engineered cell lines with homozygous knockout (KO) of glycosyltransferases using CRISPR/Cas9 and homology-directed repair (HDR) templates designed for efficient screening of clones. Strategies are provided for the stable introduction of mucin expression cassettes into the HEK293-F genome and the subsequent isolation of high-expressing cell populations. The high-titer production of recombinant mucins in conventional shaker flasks is described as an example production strategy using these cell lines.

Replacing Formalin with Phenoxetol as an Immersion Medium in a Human Cadaveric Storage Facility – A Feasibility Study

Abstract Background and Objectives: Formaldehyde is a well-known preservative commonly used to preserve anatomical specimens. However, due to concerns regarding its detrimental effects, phenoxetol has been explored for its role as an immersion agent. Hence, we aim to understand the properties of phenoxetol-based solution when used as an immersion medium for preserving gross anatomy specimens. Materials and Methods: Two sets of specimens were procured from the cadavers embalmed in formalin and one set of specimens was immersed in formalin-based solution and the other set in 1% phenoxetol. Any sloughing, degradation, or presence of mold in the specimen was recorded over 3 years. Microbial cultures were taken from the tissues as well as the immersion solutions. Qualitative feedback regarding the experience of working with the fluids was obtained from the personnel involved in handling the specimens. These specimens were displayed at the end of 3 years to the teaching faculty and the parameters such as the general appearance, consistency to touch, state of degradation, and the ability to differentiate specific structures on the specimen were elicited and statistically analyzed. Results: The parameters such as tissue preservation, haptic and visual properties, and the differentiability of the structures in the specimens were preserved adequately with phenoxetol. Microbial assay from the tissues and the fluid showed no growth. There was no significant negative feedback against its use from the staff handling the specimens. Conclusion: 1% phenoxetol is a good alternative form of immersion medium for anatomical specimens with less toxicity and better environmental impact.

Inductive Link Prediction via Interactive Learning Across Relations in Multiplex Networks

Network embedding is an important class of link prediction methods, which can use the distance between learned low-dimensional node representations to characterize the similarity between nodes. Traditional network embedding methods focus on single-layer networks, while in reality, a large part of complex networks are not isolated, but interdependent and interrelated, forming multiplex complex networks. Also, how to effectively exploit layer correlations in multiplex networks to learn more robust and valuable representations, to improve link prediction performance, has been a hot research topic in the field of complex network analysis. However, previous studies mainly focus on inferring intralinks in each layer of complex networks or anchor links among layers. Another issue that has not been discussed is how to predict potential links or reconstruct the network in unobserved relations based on existing multiplex networks. To this issue, we define a novel inductive link prediction problem in multiplex networks, in which most existing multichannel network embedding methods fail to solve. This is either because they only emphasize the specific structure information of an individual layer or only capture the common information for all layers. To effectively address this problem, we propose a novel embedding method termed interactive learning across relations (ILAR), to capture and fully exploit the multiple relations and complex layer correlations in multiplex networks. We leverage two convolutional modules and ILAR to capture the sufficient complementary and correlations in multiplex networks. Moreover, during interactive learning, a disparity constraint is introduced, which enforces the features encoded from two convolutional modules to be different and prevents information redundancy. Finally, the extensive experiments in several real-world datasets show that our model can significantly outperform the existing state-of-the-art network embedding methods on the novel link prediction problem in multiplex networks.

Analysis and experimental study on flow characteristic and rangeability of plug control valve

According to the specific structure of the plug valve, a method for estimating the rated flow coefficient was proposed. Based on a variable diameter flow passage model, flow coefficient tests for four plug control valves with different specifications were carried out, and the experimental data were analyzed and researched. The results show that: (1) The proposed estimation method for calculating the rated flow coefficient of the plug valve, in which the deviation range is -6.58%∼4.10%, meets the requirement of the standard IEC 60534-2-4-2009 as not exceeding ±10%; (2) Due to the manufacturing errors, the actual inherent flow characteristic of the plug control valve is between equal percentage and parabola. The larger the flow capacity is, the more equal the flow characteristic gets; (3) The plug control valve has an outstanding feature of high rangeability, which can be up to R=979 and is obtained based on the flow coefficient test data.

The Future of UAVs in Urban Air Mobility: Public Perception and Concerns

This study aims to examine the public perception of Unmanned Aerial Vehicles (UAVs) in the context of Urban Air Mobility (UAM) and evaluate the widespread acceptance and applicability of this new technology. In this study, semi-structured interviews were conducted with 82 participants residing in the provinces of Istanbul and Ankara. The data obtained were analyzed using the thematic analysis method to categorize participants' views about UAVs in a specific structure. As a result of this analysis, participant views were categorized into three main themes: positive views, negative views, and neutral perspectives. Among the positive views, participants' expectations regarding the emergency use of UAVs stand out. In the negative views, concerns about the security and potential risks of UAVs took the lead, while mixed thoughts about the impact of UAVs on society, legal regulations, and technological development formed the neutral category.

STRUCTURE ET DISTRIBUTION DE LA POPULATION DE BALANITES AEGYPTIACA (L.) DEL. DANS LA COMMUNE RURALE DE SIMIRI (NIGER)

In the context of the rampant destruction of natural resources, a study on the populations of Balanites aegyptiaca was carried out in Simiri. The objective of the study is to improve the understanding of the natural and anthropogenic ecological processes which are determining the distribution and structure of these populations. The survey device used is systematic with 4 transects of 2 km each arranged radially around the villages. Analysis of vegetation data shows that the floristic diversity of the area is declining. A total of 26 species divided into 19 genera and 12 families are inventoried. The most represented families are Combretaceae and Mimosaceae with 23.08% each. The population is dominated by Combretum glutinosum (30.26%), Balanites aegyptiaca (16.59%), Acacia albida (10.85%), Ziziphus mauritiana (9.35%), Guiera senegalensis (8.29%) and Piliostigma reticulatum (5.84%). The spatial distribution of Balanites aegyptiaca is under the influence of natural dynamic processes and anthropogenic actions with great variability in its density both at the village scale and at the scale of geomorphological and land use units. Furthermore, their specific structure is recurrently approved, which gives them great ecological importance in the municipality. The demographic structure of Balanites aegyptiaca, for its part, showed the population, an appreciable proportion of young individuals, and aging populations and thus confirms the importance of this woody resource. However, renewal (92%) constitutes, at the current state, the structural problem because of the strong competition for the seed.

Site Specific Non-Linear Ground Response Analysis and Soil Structure Interaction Study of Newly Constructed Dharahara Tower

This study investigates the seismic performance of the recently reconstructed Dharahara tower, a historically significant monument in Nepal that has experienced damage in past earthquakes and was completely destroyed in the recent Gorkha earthquake. Ground Response Analysis (GRA) and Soil-Structure Interaction (SSI) investigations are conducted to assess its structural behavior. GRA includes free field and structure-influenced analyses, with subsequent comparative assessment. Additionally, 3D non-linear finite element analysis is employed to derive equivalent spring constants representing soil and foundation characteristics, which are incorporated into the SSI analysis. These computed spring constants are used to model support conditions, allowing for an evaluation of the super-structural response. The study also employs non-linear dynamic analysis to compare structural responses between fix-based and spring-based models. The results indicate that the presence of the structure significantly influences surface wave motion amplification in GRA, resulting in peak ground accelerations (PGAs) that exhibit de-amplification in the free field and amplification in the presence of the structure. Furthermore, the introduction of an equivalent spring system in the soil-structure model changes the system's vibration period and damping characteristics, leading to enhanced dynamic response compared to the fixed-base model.

Consuming Less as a Rational Choice: A Review of Applied Social Exchange Theory in Topical Research on Sustainable Consumption

Along with a fast expansion in volume, research on sustainable consumption is confounded with inconsistencies in definitions and terminology. For a better categorization and thus utilization of the knowledge already accumulated in the field, a synthetic approach outlining conceptual differences and commonalities within the multitude of research appears useful. Contributing to this conceptual approach, this article pursues the role of the paradigm of rational choice in topical (2019 – 2023) research on sustainable consumption. By way of a theory-based review, the particular areas of sustainable consumption research in which social exchange is applied are scrutinized, highlighting specific structures and inclinations in terms of the history of ideas. The findings point out a preference towards the sociological take on social exchange theory (as opposed to the socio-psychological) and demonstrate that the application of social exchange theory is significant particular in research on sharing economy. On the other hand, no application of social exchange theory in areas of sustainable consumption like minimalism, downshifting or voluntary simplicity could be documented.

К ВОПРОСУ О СТАНОВЛЕНИИ СИММЕТРИИ И ПОЛЯРНОСТИ В ЭМБРИОИДОГЕНЕЗЕ IN VITRO И ЭМБРИОГЕНЕЗЕ IN VIVO

The article provides a brief overview of the literature and our own works devoted to the peculiarities of polarity and symmetry establishment during the formation of a specific three-dimensional structure of embryos in vivo and embryoids in vitro. Particular attention is paid to androclinic embryoidogenesis in vitro, as a promising model system for studying various aspects of plant morphogenesis. Cellular and cytophysiological factors influencing the polarity and symmetry establishment during the development of the embryo/embryoid are considered. The key role of IAA transport and distribution in embryo/embryoid formation is discussed.

Nanoshell Formation at the Electrically Charged Gas-Water Interface of Collapsing Microbubbles: Insights from Atomic Force Microscopy Imaging.

AFM imaging has revealed intriguing features when bulk nanobubbles were deposited on a positively charged substrate. Numerous spherical objects, each less than 20 nm in diameter, were observed on the substrate. These objects were adorned with noticeable, tiny protrusions, each measuring a few nanometers. These findings suggest the presence of solid shells contributing to the stability of the gas bodies. Furthermore, electrically charged microbubbles appear to play a critical role in the formation of these solid shells. The collapse of microbubbles in an electrolyte aqueous solution containing iron ions leads to a condensing ionic cloud, creating conditions necessary for solid nucleation at the interface. At the end of the collapsing process, concurrent multinucleation may result in the deposition of solid material on the interface, forming solid shells with specific structures on the surfaces. This study illuminates the phenomenon of electrically charged gas-water interfaces during microbubble collapse and highlights the generation of stabilized nanoshells in aqueous solutions without the need for chemical stabilizers.

Aging pattern of the brainstem based on volumetric measurement and optimized surface shape analysis.

The brainstem, a small and crucial structure, is connected to the cerebrum, spinal cord, and cerebellum, playing a vital role in regulating autonomic functions, transmitting motor and sensory information, and modulating cognitive processes, emotions, and consciousness. While previous research has indicated that changes in brainstem anatomy can serve as a biomarker for aging and neurodegenerative diseases, the structural changes that occur in the brainstem during normal aging remain unclear. This study aimed to examine the age- and sex-related differences in the global and local structural measures of the brainstem in 187 healthy adults (ranging in age from 18 to 70years) using structural magnetic resonance imaging. The findings showed a significant negative age effect on the volume of the two major components of the brainstem: the medulla oblongata and midbrain. The shape analysis revealed that atrophy primarily occurs in specific structures, such as the pyramid, cerebral peduncle, superior and inferior colliculi. Surface area and shape analysis showed a trend of flattening in the aging brainstem. There were no significant differences between the sexes or sex-by-age interactions in brainstem structural measures. These findings provide a systematic description of age associations with brainstem structures in healthy adults and may provide a reference for future research on brain aging and neurodegenerative diseases.

Two-dimensional directed lamellar assembly in silicon- and fluorine-containing block copolymer with identical surface energies

A block copolymer (BCP) with specific monomer structures of fluoroacrylate polymers was designed by exploiting the inorganic superhydrophobicity and low glass transition temperature of polydimethylsiloxane (PDMS). With the use of a fluorine-containing block providing a surface tension as low as that of PDMS (19.9 < γ\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\gamma$$\\end{document} < 21.5 mN/m), PDMS-b-poly(2,2,3,3,3-pentafluoropropyl acrylate) (PDMS-b-PPeFPA) copolymer was synthesized to create a volume-symmetric lamellar structure. The compositional randomness of the BCP chains adsorbed onto the substrates provided well-balanced interfacial interactions toward the overlaid PDMS-b-PPeFPA (γ\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\gamma$$\\end{document}PDMS-ads ≈ γ\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\gamma$$\\end{document}PPeFPA-ads). Under this symmetric confinement with simultaneous dual neutral interfaces, lamellar microdomains with a sub-10 nm half-pitch feature size were successfully oriented perpendicular to the interfaces at room temperature. We showed the response of the BCP films to a lateral electric field, demonstrating that the perpendicular lamellae were adaptively aligned along the electric vector within a short treatment period. Furthermore, the PDMS-b-PPeFPA system exhibited a remarkable etch contrast for O2 reactive ion etching, yielding unidirectionally aligned air–inorganic nanoarrays emanating from the perpendicular lamellae between the electrodes. This study reports a system engineering approach for conceiving highly immiscible, silicon- and fluorine-containing BCP whose components exhibit identical surface tensions (γ\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\gamma$$\\end{document}PDMS ≈ γ\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$\\gamma$$\\end{document}PPeFPA) and for generating perpendicularly oriented lamellar microdomains due to substrate neutrality.

Solution-based Supramolecular Hierarchical Assembly of Frenkel Excitonic Nanotubes Driven by Gold Nanoparticle Formation and Temperature.

Translating nature's successful design principle of solution-based supramolecular self-assembling to broad applications─ranging from renewable energy and information technology to nanomedicine─requires a fundamental understanding of supramolecular hierarchical assembly. Though the forces behind self-assembly (e.g., hydrophobicity) are known, the specific mechanism by which monomers form the hierarchical assembly still remains an open question. A crucial step toward formulating a complete mechanism is understanding not only how the monomer's specific molecular structure but also how manifold environmental conditions impact the self-assembling process. Here, we elucidate the complex correlation between the environmental self-assembling conditions and the resulting structural properties by utilizing a well-characterized model system: well-defined supramolecular Frenkel excitonic nanotubes (NTs), self-assembled from cyanine dye molecules in aqueous solution, which further self-assemble into bundled nanotubes (b-NTs). The NTs and b-NTs inhabit distinct spectroscopic signatures, which allows the use of steady-state absorption spectroscopy to monitor the transition from NTs to b-NTs directly. Specifically, we investigate the impact of temperature (ranging from 23 °C, 55 °C, 70 °C, 85 °C, up to 100 °C) during in situ formation of gold nanoparticles to determine their role in the formation of b-NTs. The considered time regime for the self-assembling process ranges from 1 min to 8 days. With our work, we contribute to a basic understanding of how environmental conditions impact solution-based hierarchical supramolecular self-assembly in both the thermodynamic and the kinetic regime.

Formation of Domains within a Lower-to-Higher Symmetry Structural Transition in CrI3.

CrI3 represents one of the most important van der Waals systems on the route to understanding 2D magnetic phenomena. Being arranged in a specific layered structure, it also provides a unique opportunity to investigate structural transformations in dimension-confined systems. CrI3 is dimorphic and possesses a higher symmetry low-temperature phase, which is quite uncommon. It contrasts with vanadium trihalides, which show a higher symmetry high-temperature phase. An explanation of this distinct behavior, together with a large cycle-dependent transition hysteresis, is still an open question. Our low-temperature X-ray diffraction study conducted on CrI3 single crystals complemented by magnetization and specific heat measurements was focused mainly on specific features of the structural transition during cooling. Our results manifest that the structural transition during cooling relates to the formation of structural domains despite the lower symmetry structure transforming to a higher symmetry one. We propose that these domains could control the size of thermal hysteresis.

The study of structure and function of human cell

Biological structure and function is the core field of life sciences, covering the complex organizational structure and various functions it undertakes. Cells are a very important structure and the basic unit of life activities, which means that without cells, all life activities cannot continue. So on this basis, we studied the function and structure of cells. The findings suggest that each organelle has a specific functional structure and that changes or mutations in the structure of these proteins or the arrangement of membrane proteins on the surface can lead to the development of related diseases, such as abnormalities in the Golgi apparatus leading to Alzheimer's disease Abnormalities in mitochondria can lead to lesions in muscles or the nervous system or even cancer, and mutations in the nucleus can cause babies to be born with various defects. Cells are the unit of life activities. Once the organelles are abnormal, different diseases will occur.

TOWARDS PSYCHOLOGICAL SUPPORT IN INTERACTIVE STROKE REHABILITATION THERAPY (EEG-FMRI STUDY)

HighlightsInteractive therapy (stimulation) of the brain has a significant potential, especially if it includes not only medical, but neurophysiological and psychological modalities. An integrated approach is required to provide complete readaptation to new life conditions and to decrease the percentage of disabilities in post-stroke patients. Abstract:Aim. The article is devoted to the psychological support in neurorehabilitation of patients with acute disorders of cerebral circulation. Interactive therapy (stimulation) of the brain is a new generation of neurofeedback characterized by a radical change in the “targets” of cognitive (adaptive, volitional) influence. These targets are specific cerebral structures and neural networks, the reconstruction of which leads to the restoration of physiological functions and behavioral (psychological) metamorphoses.Methods. The study involved 18 patients diagnosed with ischemic stroke with hemiparesis of 2 points in the hand (early recovery period). All patients received rehabilitation treatment at the clinic of the Federal Research Center for Fundamental and Translational Medicine. The rehabilitation program for patients from the main group (n = 11) was supplemented with a course of interactive brain stimulation in an MRI scanner. Functional magnetic resonance imaging (fMRI) in the biofeedback contour uses a natural intravascular tracer, a blood oxygen level dependent (BOLD) signal, as feedback. Thus, the subject included into an “interactive brain” circuit is learning to modulate and modify his cerebral networks creating the new ones, or “awakening” pre-existing ones in order to improve (or reform) his mental, sensory and/or motor functions. The subject being placed in a tomograph for a long time to learn new reparative skills is in a significant need of constant (sustainable) psychological support at all stages of the neurorehabilitation.Results. A course of interactive therapy contributed to a radical improvement in motor functions, confidence in movements, and an increase in working capacity. The analysis of fMRI data over time showed that activation of the accessory motor cortex and cerebellum on the ipsilateral side at the beginning is replaced by bilateral expansion of activity zones in the cerebellum and premotor cortex by the end of the course of interactive therapy. It also was established that the result of a stroke is a deep transformation of the intrapersonal relationships of patients. At the same time, rehabilitation has significant potential, especially if it includes not only medical, but also neurophysiological and psychological modalities. The dynamics of learning in terms of fMRI mapping should be successfully synchronized with the kinetics of psychological metrics and success scales, the influence of which, as a rule, remains beyond the scope of clinical studies.Conclusion. This study was carried out in line with the search for a self-referential psychological accompaniment that optimizes the recovery, the study of temporal and spatial correspondence between the interactive dynamics of fMRI and specific clinical symptoms.