Introduction. The key role in maintaining the competitiveness of economic systems belongs to the innovative specialization of economic activity, which diversifies economic activity. The existing methodological approaches to determining the strategic priorities of Smart Specialization of a particular region do not fully take into account the assessment of innovation and investment potential, taking into account the environmental component of sustainable development.
 Aim and tasks. The purpose of the proposed study is the formation of scientific approaches to assessing innovation and investment potential in the context of the formation of strategic regional environmental policy in the context of the methodology of Smart Specialization.
 Results. The article substantiates the methodological provisions for combining the methodology of Quintuple helix, Smart-specialization and Sustainable Development Goals. The institutional essence of investment and innovation policy of nature management at the regional level as a modern model of Quintuple helix is determined. An assessment of the innovation potential of the regions as a component of investment and innovation policy of nature management. The dynamics of implementation of environmental innovations by enterprises of Ukraine, the concentration of higher education institutions by regions, in particular, subordinate government agencies are analyzed. The number of students, specialists in higher education institutions majoring in natural sciences, employment in the field of nature management has been determined. The strengthening of the ecological component in the scientific and educational spheres is established for the further implement the borrowed knowledge in the formation of investment and innovation policy of nature management of individual regions. The natural resource potential of the country in terms of regions according to the statistical database is studied.
 Conclusions. The presented research proposes the author's vision, which allows not only to outline the dominants of Smart Specialization of кegions in order to determine the strategic priorities of investment and innovation policy of the national sphere of nature, but also to explore the uniqueness of regions based on scientific and technical potential. Implementation of the Smart Specialization Strategy, which will combine the methodology of Quintuple helix and Smart Specialization will provide an opportunity to determine the approach to the formation and implementation of state regional policy. A methodological approach to determining the dominants of investment and innovation regional environmental policy has been developed, taking into account the methodology of "Smart Specialization" and in the context of existing European experience.
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