Southern Spain Research Articles

Assessment and accumulation of microplastics in sewage sludge at wastewater treatment plants located in Cádiz, Spain

Sludge from Wastewater treatment plants (WWTPs) have been determined as a sink of microplastics (MP) removed from wastewater. The aim of this research work has been to evaluate the presence of these pollutants in the sludge of seven WWTPs (five urban and two industrial), located in southern Spain. Samples were collected in the primary, secondary and digested sludge matrixes, MPs were extracted following wet peroxide oxidation and the removal of cellulose, finally the samples were analyzed according to their abundance, size (from 100 μm to 5 mm), shape, colour, and polymer type. Subsequently, the data obtained on the WWTPs were compared, the main difference among the WWTPs and different sample points showed high heterogeneity in terms of abundance of microplastics, due to the differences in the sludge loaded, the processes and the type of sludge. The results from this study established that the most abundant shape was fibers; regarding the size, 100–355 μm fraction was the most abundant, showing that the amount of MPs increased when the size decreased. Regarding the type of polymers, 23 were identified by ATR-FTIR. Further, Acrylate, PE, EAA and PP were the most abundant found polymers. The presence of MPs in the digested sludge varied from 0.02 ± 0.006 MP g DW−1 to 57.18 ± 20.69 MP g DW−1 in the WWTP 6 (food industry) and WWTP 3 (urban city over 212,000 inhabitants), respectively; higher abundance of MPs found in the primary sludge in respect to secondary sludge; in concordance with the removal from wastewater line reported in other studies. The results obtained showed that MPs were widely present in sludge, becoming a sink of these pollutants, estimating that among 8.05 · 104 and 1.77 · 109 MPs · day−1 were loaded to sludge; therefore, these facilities act as a significant source of MPs into agriculture when sludge is used as soil amendment.

Vegetation Cover Estimation in Semi-Arid Shrublands after Prescribed Burning: Field-Ground and Drone Image Comparison

The use of drones for vegetation monitoring allows the acquisition of large amounts of high spatial resolution data in a simple and fast way. In this study, we evaluated the accuracy of vegetation cover estimation by drones in Mediterranean semi-arid shrublands (Sierra de Filabres; Almería; southern Spain) after prescribed burns (2 years). We compared drone-based vegetation cover estimates with those based on traditional vegetation sampling in ninety-six 1 m2 plots. We explored how this accuracy varies in different types of coverage (low-, moderate- and high-cover shrublands, and high-cover alfa grass steppe); as well as with diversity, plant richness, and topographic slope. The coverage estimated using a drone was strongly correlated with that obtained by vegetation sampling (R2 = 0.81). This estimate varied between cover classes, with the error rate being higher in low-cover shrublands, and lower in high-cover alfa grass steppe (normalized RMSE 33% vs. 9%). Diversity and slope did not affect the accuracy of the cover estimates, while errors were larger in plots with greater richness. These results suggest that in semi-arid environments, the drone might underestimate vegetation cover in low-cover shrublands.

Historical Approach and Scale Reconstruction of Two Medieval Mechanisms from “The Book of Secrets”

“The Book of Secrets in the Results of Ideas”, usually called “The Book of Secrets” is a codex containing drawings and descriptions of thirty-one artifacts attributed to the engineer Alī Ibn Khalaf al-Murādī, who lived in Andalusia in southern Spain at the beginning of the 11th century. This manuscript is one of the first written testimonies that describe medieval mechanisms with complex precision. The aim of this work is to reconstruct and study from a historical and technological point of view two of the ancient artifacts contained in the “Book of Secrets”, the “Fortress Demolisher” and the “Magic Well”. The “Fortress Demolisher” is a war machine designed to demolish the upper part of the walls or towers and consists of a battering ram mounted on a platform that can be raised several meters above the ground using a scissor mechanism. The “Magic Well” is a mechanism with several pulleys whose purpose is to raise water several meters from the bottom of a well by collecting a small portion of rope. To reconstruct the two artifacts, a digital model and a detailed study of the geometry and operation of the mechanisms, were first carried out. 3D printing, using the Fused Deposition Modelling technology, made the physical reproduction. The digitization and physical reproduction by 3D printing of the mechanisms represent an update of the traditional ways of transmitting knowledge. The use of these new technologies makes the knowledge more accessible, understandable and attractive.

Oxidative status in relation to blood parasite infections in house sparrows living along an urbanization gradient

Living organisms are exposed to a wide range of substances - internal and external - which act like reactive oxygen species (ROS). Oxidative damage accurs when the balance between ROS and antioxidant defenses is altered. Urbanization and parasite infection are both important sources of ROS with different harmful effects on wildlife health, but the potential synergies between both factors are poorly known. Here, we analyse the oxidative stress of wild juvenile male house sparrows (Passer domesticus) along an urbanization gradient in relation to the infection status by three common blood parasites (Plasmodium, Haemoproteus and Leucocytozoon) and bird body condition. We analysed samples from 688 birds captured at 45 localities from southern Spain grouped into triplets including an urban, a rural and a natural habitat, with 15 localities per habitat type. We measured i) thiobarbituric acid reactive substances (TBARS) levels as indicator of the oxidative damage to lipids, and the activity of three antioxidant enzymes ii) glutathione peroxidase (GPx), iii) superoxide dismutase (SOD) and iv) glutathione reductase (GR) as indicators of bird's antioxidant capacity. Birds infected with Haemoproteus and urban birds showed significantly and marginally higher levels of TBARS than uninfected and rural birds, respectively. The relationship between TBARS and body condition is different regarding the infection status (significative) and habitat (marginally significant) being negative for Haemoproteus infected and urban birds but positive for uninfected and non-urban birds. The antioxidant activity was significantly lower in Plasmodium infected birds but marginally higher in Leucocytozoon infected birds than in uninfected ones. Individuals with higher body condition had higher GPx and SOD activity in relation to a lower GR activity. Overall, these results suggest that blood parasites infections and urbanization affect the oxidative status of wild birds and highlight the role of bird's body condition on the regulation of the oxidative stress status.

Footprints of COVID-19 on Pollution in Southern Spain

Background: Many annual deaths in Spain could be avoided if pollution levels were reduced. Every year, several municipalities in the Community of Andalusia, located in southern Spain, exceed the acceptable levels of atmospheric pollution. In this sense, the evolution of primary air pollutants during the March–June 2020 lockdown can be taken as reliable evidence to analyze the effectiveness of potential air quality regulations. Data and Method: Using a multivariate linear regression model, this paper assesses the levels of NO2, O3, and PM10 in Andalusia within the 2017–2020 period, relating these representative indices of air quality with lockdown stages during the pandemic and considering control variables such as climatology, weekends, or the intrusion of Saharan dust. To reveal patterns at a local level between geographic zones, a spatial analysis was performed. Results: The results show that the COVID-19 lockdown had a heterogeneous effect on the analyzed pollutants within Andalusia’s geographical regions. In general terms, NO2 and PM10 concentrations decreased in the main metropolitan areas and the industrial districts of Huelva and the Strait of Gibraltar. At the same time, O3 levels rose in high-temperature regions of Cordoba and Malaga.

Exploring biocontamination in associated macrofaunal assemblages in marinas: Soft bottoms vs artificial hard substrate. Where and what to look for?

Marinas are hot spots of non-indigenous marine species (NIS). Introductions of NIS, regardless of their actual or potential impacts, can be considered as a biocontamination of the ecosystem. To characterize the biocontamination status of a marina it is important to know which habitats and major taxa can provide the most useful information. To address this issue, we analyze the associated macrofaunal community associated to soft substrate (sediment; SS) and artificial hard substrate (lateral surface of pontoons; AHS) of six marinas from Southern Spain. 9 NIS were found, including 8 crustaceans (7 peracarids) and 1 polychaete; 8 NIS were found on AHS and 4 in sediments. Indeed, site-specific BioContamination Index (SBCI) showed higher values in AHS than SS. Our results indicate peracarids in AHS should be the primary target for assessing the level of biocontamination in marinas. This may help scientists and environmental managers to stablish strategies for monitoring marine bioinvasions.

The Adolescent Problem Gambling Prevalence Associated with Leisure-Time Activities and Risky Behaviors in Southern Spain

Gambling addiction is increasing and is becoming a public health concern due to the rise of gambling-related harms affecting the youth. Previous studies suggest a strong link between problem gambling (PG) and substance use and psychosocial and familial factors. Our main objective was to analyze the association between PG and factors like sport, leisure-time activities, and risk-taking behaviors in adolescents. A survey on substance use and addictive behaviors was performed in 2019 on a representative sample of 2240 subjects (14–18 years) from the Southern Spain Region. Data variables like socio-demographic characteristics, sport, leisure and free time activities, family environment, PG (Lie-Bet Scale), compulsive internet use (CIUS Scale), and consumption of alcohol and cannabis were collected using a standardized questionnaire. Weighted PG prevalence was estimated in either sex, as well as the differences between various levels of PG using chi-square tests. Crude and adjusted weighted logistic regression models were used to identify predictors associated with PG. The prevalence of PG was associated with shopping frequency, compulsive internet use, cannabis use in the previous month, higher family economic status, and having a homemaker father which increased the likelihood of PG. On the contrary, cultural hobbies such as playing an instrument, painting, singing, and writing, and having a working mother were inversely associated with PG. Our results suggest that encouraging participation in creative activities along with supervised shopping and monitoring compulsive internet use and cannabis consumption may contribute some protection against adolescent PG.

Discovery of the male of Tethysbaena tarsiensis Wagner, 1994 (Peracarida, Thermosbaenacea, Monodellidae)

Abstract The male of Tethysbaena tarsiensis Wagner, 1994 is described from the Guadalquivir drainage in southern Spain. The original description was based on a sample containing only female specimens. As males of this species were unknown thus far, additional descriptive notes are added to the original diagnosis by Wagner (1994). Additional remarks on the habitat of the species are made as well.

Digital social networks as tools of citizen movements

Faced with the environmental plundering resulting from the tourism boom in southern Spain, a number of groups have emerged to promote the defense of the natural heritage. In Malaga, capital of the "Costa del Sol", eight citizen platforms have been detected that use digital tools to amplify their demands. This study analyzes the strategy used by these movements in order to assess the planning of their presence on Facebook. The methodology used was the content analysis of this digital social network and the qualitative analysis of the results obtained, as well as semi-structured interviews with the leaders of these groups.

Multiomic Data Integration in the Analysis of Drought-Responsive Mechanisms in Quercus ilex Seedlings.

The integrated analysis of different omic layers can provide new knowledge not provided by their individual analysis. This approach is also necessary to validate data and reveal post-transcriptional and post-translational mechanisms of gene expression regulation. In this work, we validated the possibility of applying this approach to non-model species such as Quercus ilex. Transcriptomics, proteomics, and metabolomics from Q. ilex seedlings subjected to drought-like conditions under the typical summer conditions in southern Spain were integrated using a non-targeted approach. Two integrative approaches, PCA and DIABLO, were used and compared. Both approaches seek to reduce dimensionality, preserving the maximum information. DIABLO also allows one to infer interconnections between the different omic layers. For easy visualization and analysis, these interconnections were analyzed using functional and statistical networks. We were able to validate results obtained by analyzing the omic layers separately. We identified the importance of protein homeostasis with numerous protease and chaperones in the networks. We also discovered new key processes, such as transcriptional control, and identified the key function of transcription factors, such as DREB2A, WRKY65, and CONSTANS, in the early response to drought.

An Asp376Glu substitution in ALS gene and enhanced metabolism confers high tribenuron-methyl resistance in Sinapis alba.

Acetolactate synthase (ALS) inhibiting herbicides (group 2) have been widely applied for the last 20 years to control Sinapis alba in cereal crops from southern Spain. In 2008, a tribenuron-methyl (TM) resistant (R) S. alba population was first reported in a cereal field in Malaga (southern Spain). In 2018, three suspected R S. alba populations (R1, R2 and R3) to TM were collected from three different fields in Granada (southern Spain, 100 km away from Malaga). The present work aims to confirm the putative resistance of these populations to TM and explore their resistance mechanisms. Dose-response assays showed that the R1, R2 and R3 populations ranging between 57.4, 44.4 and 57.1 times more resistance to TM than the susceptible population (S). A mutation in the ALS gene (Asp376Glu) was detected in the Rs S. alba populations. 14C-metabolism studies show that metabolites and TM were changing significantly faster in the R than in the S plants. Alternative chemical control trials showed that 2,4-D and MCPA (auxin mimics), glyphosate (enolpyruvyl shikimate phosphate synthase,EPSPS, inhibitor-group 9), metribuzin (PSII inhibitors/Serine 264 Binders, -group 5) and mesotrione (hydroxyphenyl pyruvate dioxygenase, HPPD, inhibitor-group 27) presented a high control of the four populations of S. alba tested, both S and R. Based on these results, it is the first case described where the Asp376Glu mutation and P450-mediated metabolism participates in resistance to TM in S. alba. Comparing these results with those found in the S. alba population in Malaga in 2008, where the resistance was TSR type (Pro197Ser), we can suggest that despite the geographical proximity (over 100 km), the resistance in these cases was due to different evolutionary events.

Mixed-Layer Illite-Smectite Illitization under Supercritical CO2 Conditions

The long-term safe storage of CO2 in geological reservoirs requires the understanding of the impact of CO2 on clay-rich sealing cap rocks. The reactivity of the mixed layer of illite-smectite was investigated to determine the reaction pathways under conditions of supercritical CO2 (scCO2) conditions in the context of geological CO2 storage. A common clay (blue marl from the Guadalquivir Tertiary basin, southern Spain) was tested under brine scCO2 conditions (100 bar and 35 °C) for 120 and 240 h. The clay sample (blue marl) contains calcite, quartz, illite, smectite, and the corresponding mixed-layer and kaolinite. X-ray diffraction (XRD), Brunauer–Emmett–Teller (BET), and inductively coupled plasma optical emission spectroscopy (ICP-OES) analyses were performed. The illitization of mixed-layer illite-smectite was observed by XRD and confirmed by a variation in the content of different elements (K, Mg, Na, Ca, and Fe) of the transformation, as well as an increase in the specific surface (SSA) of the clay (36.1 to 38.1 m2/g by N2, 14.5 to 15.4 m2/g by CO2 adsorption). Furthermore, these reactions lead to mineral dissolution and secondary mineral formation along the CO2–water–clay intercalations of the source rock were responsible for a change in porosity (7.8 to 7.0 nm pore size). The implications of illitisation, mineral destruction, and precipitation processes on CO2 storage and clay layer integrity should be explored before deciding on a geological storage location.

Sport fishing and vessel pressure on the endangered cetacean Delphinus delphis. Towards an international agreement of micro-sanctuary for its conservation

Delphinus delphis Linnaeus 1758 is considered an endangered species in the Mediterranean. The species is resident in the Bay of Algeciras - Gibraltar (southern Spain; waters partially shared with Gibraltar, UK). This area is subject to strong anthropogenic pressure, but with an input of renewed waters in its central area. During three consecutive years (March 2017–March 2020), sightings were observed concentrated along the submarine canyon that runs north to south across the bay, forming a “specular S″ with a maximum depth of 460 m. The pods of Dd showed a continuous or semi-continuous spatial distribution along the vertical axis of the submarine canyon, where they feed, mate, breed and rear calves in a well-defined area that constitutes a critical habitat. This area has been cartographically delimited, choosing the area of maximum dolphin frequency as highly vulnerable due to the possibility of collisions with small vessels (<15 m) and interactions with sport fishing activities (popping, ballooning, trolling) which constitute the main threat counting the derived impacts from noise pollution. Spatio-temporal mapping has been developed to visualize the overlapping between the dolphin groups and small vessels. Mapping analysis has shown that an international Spanish-British micro-sanctuary urgently needs to be established in order to create a protected area specifically for the common dolphins, and for other species which inhabit/use the waters between Spain and Gibraltar. Coordinates and a delimited surface area have been proposed in this paper.

Factors that characterize oleotourists in the province of Córdoba.

Oleotourism (olive oil tourism) is a new form of gastronomic tourism that satisfies increasingly challenging tourist demand, especially in the wake of the pandemic and for tourists who seek not only quality food products but also a safe environment to enjoy their chosen activity. Córdoba is a province in southern Spain where olives play a very important role, accounting for 50% of its cultivated area, and whose agricultural activity can be complemented with tourism due to its 189 oil mills that can welcome visitors for oil tasting. However, this type of tourism is not seeing an expected boom. This research analyzes, through a varimax analysis, the factors that attract and drive oleotourists as well as the components of such tourism. As a result, four principal components related to tourists and tourism offers were obtained, finding a high degree of satisfaction of oleotourist with the routes explored as well as a lack of knowledge of this type of tourism in international markets.

Trace and rare earth element distribution in hyperalkaline serpentinite-hosted spring waters and associated authigenic carbonates from the Ronda peridotite

The Ronda peridotite in southern Spain is subject to low-temperature serpentinization by circulating groundwaters that produces hyperalkaline fluids of high pH and low dissolved inorganic carbon content (DIC). When these waters resurface as hyperalkaline springs they take up atmospheric carbon dioxide (CO2), which triggers the precipitation of travertine carbonates. Spring waters at Baños del Duque, Fuente Romana, and Fuente Amargosa show typical chemical features of hyperalkaline springs such as high pH and low DIC, yet exhibit an unusual distribution of rare earth elements (REEs) enriched in lanthanum (La) and cerium (Ce). In order to constrain changes in spring water chemistry and carbonate precipitation during atmospheric CO2 uptake, a reaction path model was set up to illustrate that the distribution of REE species in the high pH and low DIC water is strongly dependent on REE complexation by hydroxide and carbonate species. Reaction path modeling results show that during spring fluid emission and CO2 uptake, REE speciation in the fluids shifts from a dominance of hydroxide to carbonate complexes. The complexation of rare earth elements in the high pH, low DIC fluids strongly depends on the DIC content of the respective fluid. The REE distribution of travertine deposits associated with the hyperalkaline springs deviates from that in the fluids, suggesting that travertines precipitated from fluids different to those currently emitted from the springs.

Geomorphic signature of segmented relief rejuvenation in the Sierra Morena, Betic forebulge, Spain

Abstract. The foreland relief of alpine orogenic belts is often rejuvenated due to the intraplate propagation of orogenic deformation. Thus, in these long-lived areas, the localisation of relief rejuvenation may be largely controlled by the reactivation of previous mechanical discontinuities. In this regard, we explored the relationship between the relief rejuvenation pattern and the distribution, geometry, and kinematics of faults in a wide segment of the Betic foreland (Sierra Morena, southern Spain). Specifically, we focused on the forebulge, a WSW–ENE flexural relief that formed, paired to the Betic foreland basin, in response to orogenic load. For this purpose, we applied both qualitative and quantitative geomorphological tools, including geomorphic indices and knickpoint pattern modelling in χ space. We found that the pattern of relief rejuvenation responds to large-scale flexural uplift coupled with the tectonic activity of two groups of faults that often show evidence of reactivation, namely overall WSW–ENE faults contributing to both regional NNW–SSE relief segmentation and vertical extrusion of the forebulge, and NW–SE reverse faults associated with an outstanding WSW–ENE topographic segmentation in the west of the study area. In addition, our knickpoint modelling suggests that the faults related to the southernmost Sierra Morena mountain front have been particularly active in recent times, although their activity span and the relative uplift that they accommodate differ along the Sierra Morena/foreland basin limit. The knickpoint pattern also suggests a significant reorganisation of the analysed drainage basins. The strain partitioning accommodated by the structures involved in relief rejuvenation suggests the intraplate propagation of the transpressional deformation reported from the Betic external fold and thrust belt.

Factors Driving Autumn Quercus Flowering in a Thermo-Mediterranean Area

The flowering period of plants is a critical time since it determines their reproductive success. Flowering is controlled by different factors including genetic regulation and environmental conditions. In the Mediterranean area, favourable conditions usually occur in spring, when most plant species flower including those of the Mediterranean Quercus genus. This paper reveals and analyses an unusual and lesser-known phenomenon occurring in the two main Mediterranean agroforestry ecosystems of South Europe, the Mediterranean forest and “dehesa”, that is, a second flowering occurring in autumn for the species Quercus ilex subsp. ballota (holm oak). The continuous pollen monitoring of the atmosphere in the city of Cordoba (southern Spain) for 25 years, together with field phenological observations in the area, has indicated that, apart from the main pollination period in spring, secondary flowerings also occasionally occur in this area, specifically in autumn. The present work examines these uncommon pollination events detected in the autumns of certain years with the aim of determining the main environmental factors that influence and control them. During the 25-year study period, there were 7 years in which a secondary Quercus flowering was detected in the area from the second half of October until the end of November. The univariate statistical analysis of the influence of environmental variables determined that the meteorological conditions in September were the most influential. Low mean temperatures, together with record rainfall in that month, led to autumn flowering events. The phenological characteristics of the spring pollen season were also influential. In the years with a shorter spring, the Quercus pollen season tended to present autumn flowerings. A multivariate adaptive regression splines (MARS) model was built to explain the effects of the different variables on the occurrence of autumn pollination. The results indicated that the combined effect of three predicting variables, September rainfall, the length of the spring pollen season, and the end of the spring pollen season, explained 92% of the variance. The validation showed a strong relationship between the expected and the observed autumn pollen concentrations. Therefore, the present analysis of a long-term pollen database revealed that the main causes of this unusual second flowering in autumn were strongly related to climate change, i.e., strong dry summers and warm autumns. In addition, the results showed that the phenomenon was more frequent in the years with low pollination during spring due to different meteorological events potentiated by climate change, such as dryness or heavy rain episodes, as a way of ensuring acorn crops. The results explain how this unusual and lesser-known phenomenon in agroforestry dynamics is related to the adaptation to climate change and the main factors that are driving it, as well as the potential consequences for these important and endangered Mediterranean ecosystems.

Dynamics, distribution, and transformations of mercury species from pyrenean high-altitude lakes

While mercury (Hg) is a major concern in all aquatic environments because of its methylation and biomagnification pathways, very few studies consider Hg cycling in remote alpine lakes which are sensitive ecosystems. Nineteen high-altitude pristine lakes from Western/Central Pyrenees were investigated on both northern (France) and southern (Spain) slopes (1620–2600 m asl.). Subsurface water samples were collected in June 2017/2018/2019 and October 2017/2018 for Hg speciation analysis of inorganic mercury (iHg(II)), monomethylmercury (MMHg), and dissolved gaseous mercury (DGM) to investigate spatial and seasonal variations. In June 2018/2019 and October 2018, more comprehensive studies were performed in four lakes by taking water column depth profiles. Besides, in-situ incubation experiments using isotopically enriched Hg species (199iHg(II), 201MMHg) were conducted to investigate Hg transformation mechanisms in the water column. While iHg(II) (0.08–1.10 ng L−1 in filtered samples; 0.11–1.19 ng L−1 in unfiltered samples) did not show significant seasonal variations in the subsurface water samples, MMHg (<0.03–0.035 ng L−1 in filtered samples; <0.03–0.062 ng L−1 in unfiltered samples) was significantly higher in October 2018, mainly because of in-situ methylation. DGM (0.02–0.68 ng L−1) varies strongly and can exhibit higher levels in comparison with other pristine areas. Depth profiles and incubation experiments highlighted the importance of in-situ biotic methylation triggered by anoxic conditions in bottom waters. In-situ incubations confirm that significant methylation, demethylation and photoreduction extents are taking place in the water columns. Overall, drastic environmental changes occurring daily and seasonally in alpine lakes are providing conditions that can both promote Hg methylation (stratified anoxic waters) and MMHg photodemethylation (intense UV light). In addition, light induced photoreduction is a major pathway controlling significant gaseous Hg evasion. Global warming and potential eutrophication may thus have direct implications on Hg turnover and MMHg burden in those remote ecosystems.

Networking experiences for promoting HIA implementation in Andalusia

Abstract The Health Impact Assessment (HIA) is a useful tool that allows policymakers and project developers to predict and control the consequences of their proposals on the health and equity of the affected population. The existence of a legislative framework for the institutionalization of HIA has been proposed as a critical factor in providing permanent rules and in legitimizing HIA within the decision-making process. In Andalusia (Southern Spain), the HIA is legally binding after the approval of the Public Health Act 16/2011 and Decree 169/2014, which establishes the HIA procedure in the Andalusian context. This new legal framework made necessary to promote capacity-building programs in HIA, but also to develop an online platform that would allow sharing experiences and knowledge among all those public health professionals involved in the review and approval of HIA reports submitted by the developers of proposals. These tasks were addressed by a leading group of the HIA network made up of officials from the Regional Ministry of Health and experts from the Andalusian School of Public Health (EASP). The platform, set up in 2018, integrates around 90 professionals from different administrative bodies according to the proposal's scope (local, regional), covering the great geographical dispersion of Andalusia, and allowing to evaluate more than 2400 proposals in 4 years. The platform also facilitates the traceability of files (both for improving internal management and in responding to external queries), and acts as a source of relevant evidence, supporting the work of professionals involved in HIA. The platform allows extracting data and generating figures, showing information on the number of files created and resolved by different periods, provinces, type of proposals, etc. This tool has been very useful for the continuous training of professionals involved in HIA in Andalusia, following a coaching approach. Speakers/Panellists Robert Otok ASPHER, Brussels, Belgium

Social inequalities in multimorbidity patterns in southern Spain: findings from the DEMMOCAD survey

Abstract Background Multimorbidity (MM) is associated with lower quality of life, greater disability, and higher use of health services. It is a complex problem that is difficult to capture due to the broad spectrum of concurrent chronic diseases involved. There is a need to identify and characterize patterns of chronic conditions in the local context of specific population groups. The DEMMOCAD project aims to respond to this knowledge gap by detecting patterns of MM and their inequalities in the province of Cadiz (Spain). Methods A cross-sectional study was carried out by means of telephone interviews with people over 50 years of age. The final sample was 1592 individuals with MM. A latent class analysis was carried out to identify patterns of MM from 31 chronic conditions. First, the appropriate number of classes was established, considering model fit indices, class size, and clinical interpretability. Subsequently, covariates were introduced into the model using the three-step approach, a technique that minimizes biases in the multinomial regression model. Results Preliminary analyses of the goodness-of-fit indices of the model derived five MM patterns (entropy = 0.727): (C1) mild MM; (C2) cardiovascular; (C3) musculoskeletal; (C4) musculoskeletal plus mental; and (C5) complex MM. Compared with class C1, persons in class C5 were significantly older and less educated, class C4 had a lower income, class C3 was smokers and disabled, and class C2 was characteristic among older males. All four classes also showed lower scores on mental and physical dimensions of the SF12 scale compared to class C1. Conclusions In addition to providing an adjusted characterization of the population of the area analyzed, these initial findings highlight the existence of social inequalities in multimorbidity at the local level that should be addressed by implementing policies targeting the most vulnerable groups in Cadiz (low socioeconomic status groups, people with disabilities, and the elderly). Key messages