Southern Spain Research Articles

Association between Single Nucleotide Polymorphisms Related to Vitamin D Metabolism and the Risk of Developing Asthma.

Asthma is a chronic non-communicable disease that affects all age groups. The main challenge this condition poses is its heterogeneity. The role of vitamin D in asthma has aroused great interest, correlating low vitamin D levels and polymorphisms in the genes involved in its metabolic pathway with the risk of asthma. The aim of this study was to evaluate the influence of 13 single nucleotide polymorphisms (SNPs) related to the vitamin D metabolism on the susceptibility to asthma. An observational case-control study was performed, including 221 patients with asthma and 442 controls of Caucasian origin from southern Spain. The SNPs CYP24A1 (rs6068816, rs4809957), CYP27B1 (rs10877012, rs4646536, rs703842, rs3782130), GC (rs7041), CYP2R1 (rs10741657) and VDR (ApaI, BsmI, FokI, Cdx2, TaqI) were analyzed by real-time PCR, using TaqMan probes. The logistic regression model adjusted for body mass index revealed that in the genotype model, carriers of the Cdx2 rs11568820-AA genotype were associated with a higher risk of developing asthma (p = 0.005; OR = 2.73; 95% CI = 1.36-5.67; AA vs. GG). This association was maintained in the recessive model (p = 0.004). The haplotype analysis revealed an association between the ACTATGG haplotype and higher risk of asthma for the rs1544410, rs7975232, rs731236, rs4646536, rs703842, rs3782130 and rs10877012 genetic polymorphisms (p = 0.039). The other SNPs showed no effect on risk of developing asthma. The Cdx2 polymorphism was significantly associated with the susceptibility of asthma and could substantially act as a predictive biomarker of the disease.

Streptomyces isolated from bird feathers as a potential source for novel antibiotics.

Streptomyces, the main source of antibiotics essential for human health, are widely distributed in nature among terrestrial, oceanic and atmospheric environments. New trends in antibiotic discovery are focused in the search for novel bioactive strains in unexplored habitats. We provide here evidence of the presence of diverse Streptomyces populations in wild bird feathers, such as the seagull, Larus michahellis, collected at Northern Spain; the sparrow, Passer domesticus, and the hoopoe, Upupa epops, both collected in Southern Spain. Taxonomic identification of fourteen bioactive strains, by sequencing their 16S rRNA gene and phylogenetic analyses, revealed that all of them are homologous to a total of 10 different Streptomyces. Strains from seagull samples are homologous to other antibiotic producers previously isolated from atmospheric, marine and terrestrial environments in the Cantabrian Sea region, Northern Spain. Isolates form Southern feather samples, from a house sparrow and a Eurasian hoopoe, are homologues to Streptomyces strains previously isolated mainly from soils along the Mediterranean region. The most relevant feature is that they are producers of diverse antibiotics with activity against Gram-positive, Gram-negative bacteria and fungi. We report here the successful activation of silent antibiotic biosynthetic pathways in response to changes in environmental conditions, such as incubation temperature and salinity of the culture medium, in agreement with the OSMAC approach, One Strain Many Compounds. The finding of bioactive Streptomyces in bird's plumage might be of relevance, not only in the ecology of Streptomyces-birds associations, but also in medicine and biotechnology since they can be regarded as a potential source for novel antibiotics.

Analysis of Water Pricing Policy Effects in a Mediterranean Basin Through a Hydroeconomic Model

This paper explores the impacts of water pricing as a demand management policy, at a regional level (or basin-sector). To this aim, a hydro-economic model for the Guadalquivir River basin (southern Spain) is proposed here. This basin constitutes a perfect example of a Mediterranean basin subject to frequent and long drought periods, where challenges related to water scarcity are increasing, leading to social conflicts among water users. Moreover, this basin is characterised by a closure state meaning that all available water resources are already allocated among users. In this context, water pricing policy may act as an effective tool to reduce water demand by encouraging changes of behaviour in water users. In particular, those who perform irrigation practices in the agricultural sector. This paper focuses on the irrigation sector since it is the main water user in the basin (87%). Additionally, alternative water-availability scenarios have been used to test the effect of water pricing under drought conditions. The hydro-economic model presented here has been sectorized into four basin sectors with common characteristics (hydro and economic). This enables the analysis of alternative price scenarios in the agricultural sector, in terms of water used, crop patterns and gross margin. Results show that water pricing policy should consider the regional characteristics at the basin-sector scale to gain effectiveness and equity at the river basin scale. Moreover, it has been found that both water availability and the crop pattern at the basin-sector scale have an effect on the reduction of water used (and therefore in gross margin.

Factors affecting populations of the endemic Iberian hare (Lepus granatensis) after the first myxomatosis outbreaks in Central Spain.

The Iberian hare (Lepus granatensis) is an endemic species distributed in Spain and Portugal. Myxomatosis outbreaks affecting this species were detected in 2018 in Central and Southern Spain, spreading afterward. Aiming to evaluate factors affecting the status of hare population after the arrival of myxomatosis, we conducted 108 nocturnal hare counts in Central Spain during two study periods (winter/spring and summer/autumn) in 54 different hunting grounds, covering 1071 km and observing 884 individuals. The mean density in winter/spring was 7.66 hares/100 ha, (range 6.14-9.54/100 ha), while in summer/autumn, it was 3.4 hares/100 ha (range 2.6-4.4/100 ha). Densities of hares were not affected by the dominant habitat and the presence/absence of myxomatosis outbreaks. Hares were more abundant at hunting grounds at a higher altitude and in those conducting targeted management, while detection of myxomatosis was related to lower altitude and higher levels of game management. A MaxEnt model used to generate a risk map for myxomatosis occurrence showed that the temperature annual range was the most important predictor, which suggests that environmental factors affecting myxomatosis vectors (mosquitoes, fleas, and ticks) could play a key role in disease transmission. As myxomatosis in hares is becoming endemic, hare densities may be improved by game management and the monitoring and surveillance of this emerging disease. These surveillance programs could be the basis of effective collaborations between hunters, researchers, and environmental managers.

Impact of the first wave of the COVID-19 pandemic on non-COVID inpatient care in southern Spain

We assessed the impact of the first wave of COVID-19 pandemic on non-COVID hospital admissions, non-COVID mortality, factors associated with non-COVID mortality, and changes in the profile of non-COVID patients admitted to hospital. We used the Spanish Minimum Basic Data Set with diagnosis grouped according to the Diagnostic Related Groups. A total of 10,594 patients (3% COVID-19; 97% non-COVID) hospitalised during the first wave in 2020 (27-February/07-June) were compared with those hospitalised within the same dates of 2017–2019 (average annual admissions: 14,037). We found a decrease in non-COVID medical (22%) and surgical (33%) hospitalisations and a 25.7% increase in hospital mortality among non-COVID patients during the first pandemic wave compared to pre-pandemic years. During the officially declared sub-period of excess mortality in the area (17-March/20-April, in-hospital non-COVID mortality was even higher (58.7% higher than the pre-pandemic years). Non-COVID patients hospitalised during the first pandemic wave (compared to pre-pandemic years) were older, more frequently men, with longer hospital stay and increased disease severity. Hospitalisation during the first pandemic wave in 2020, compared to hospitalisation during the pre-pandemic years, was an independent risk factor for non-COVID mortality (HR 1.30, 95% CI 1.07–1.57, p = 0.008), reflecting the negative impact of the pandemic on hospitalised patients.

Urban Heritage Rehabilitation: Institutional Stakeholders’ Contributions to Improve Implementation of Urban and Building Regulations

Climate change, natural hazards, and human actions are threatening cultural heritage in urban areas. More than ever, building regulations’ procedures and criteria are essential to guarantee the protection and safeguarding of urban areas and their buildings. These procedures and criteria are crucial to assist stakeholders in decision-making, especially when facing rapid transitions and transformative changes in urban heritage areas. Several institutional stakeholders in charge of urban heritage protection strengthen the need for a better implementation of building regulations through flexible criteria to support intervention procedures in buildings with different features and in different contexts. Under this topic, the present study uses a twofold method. Firstly, the authors analyze and compare the urban and building regulations of three Southern European countries, Italy, Spain, and Portugal, concerning procedures and criteria directed to the built heritage; secondly, they highlight and compare the views of different institutional stakeholders from the same three countries, at different levels (national, regional, and municipal), to understand the impact of the implementation of the regulations on the ground. The findings show the relevance of the institutional stakeholders’ views to improve the regulations and their practice. They highlight the need to promote inventory and cataloging procedures, as well as flexible criteria when dealing with urban heritage buildings.

Leaf Senescence of the Seagrass Cymodocea nodosa in Cádiz Bay, Southern Spain

Leaf decay in seagrasses is enhanced in some seasons since large green senescent beach-cast seagrass leaves are frequently recorded during autumn and winter seasons. Here, we explore if senescence is operating in seagrass leaf decay or if hydrodynamic stress is responsible for the seasonal leaf abscission. A seasonal study on the temperate seagrass Cymodocea nodosa was carried out in four locations with contrasting hydrodynamic regimes. The morphological, biomechanical and material properties of C. nodosa were measured. The force required to break the ligule was always lower than that required to break the blade. This could be considered an adaptive strategy to reduce acute drag forces and thus lessen the chance of plant uprooting. The absolute force needed to dislodge the blade at the ligule level varied with season and location, with the lowest forces recorded in autumn. This may indicate that senescence is operating in this species. On the other hand, the minimum estimated failure velocities for leaf abscission were also recorded in autumn. Consequently, this may cause the premature shedding of leaves in this season before the senescence process has finished and can probably explain the occurrence of green beach-cast seagrass leaves usually found during autumn and winter.

Faunal turnover between meso- and infralittoral algal turf assemblages: A case study in a highly biodiverse Marine Protected Area of the northern Alboran Sea (W Mediterranean)

The molluscan fauna inhabiting the algal turfs of mesolittoral rocky outcrops, dominated by the coralline alga Ellisolandia elongata, has been quantitatively analysed in a Marine Protected Area of Málaga, southern Spain (W Mediterranean). A total of 2520 mollusc specimens belonging to 46 species were identified. This taxocenosis was compared to that previously studied from the infralittoral level, where the alga Halopteris scoparia was prevailing and 4084 specimens belonging to 66 species of Mollusca were found. The species Pisinna glabrata, Skeneopsis planorbis, Nodulus spiralis and Tricolia miniata dominated the mesolittoral stage. The occurrence of congeneric species pairs (Tricolia miniata/Tricolia pullus, Nodulus spiralis/Nodulus contortus) which achieve high abundance in one of the levels and are scarce or absent in the other was striking. Therefore, the coralline algal turf in the lower part of the intertidal zone is not just an extension of the “Photophilous Algae” community but a benthic community of its own.

Psychometric Examination of the Body, Eating, and Exercise Comparison Orientation Measure (BEECOM) among Spanish Adolescents and Young Adults.

The Body, Eating, and Exercise Comparison Orientation Measure (BEECOM) has been frequently used within the context of research on eating disorders (ED). Although both long (BEECOM-L) and short (BEECOM-S) versions of the instrument exist, their psychometric properties have not yet been concurrently investigated across different populations in terms of age and gender. The present study aimed to compare the psychometric properties of both the BEECOM-L and the BEECOM-S among Spanish male and female non-clinical adolescents and young adults. Data from 4 samples including 1213 middle school and college students enrolled in 10 education centers from southern Spain (age ranging from 12 to 35 years, Mage = 17.796, SDage = 4.796, 53% females) were analyzed using factorial, correlation, and regression analysis techniques. Results provided evidence that support the reliability, measurement invariance according to age and gender, and convergent/incremental validity for the scores from both the BEECOM-L and BEECOM-S. Concerning factorial validity, marginally acceptable and adequate goodness-of-fit indices were obtained for the BEECOM-L and BEECOM-S, respectively. The BEECOM-S proves to be a psychometrically sound instrument with potential value for assessing social comparisons focused on body, eating, and exercise in non-clinical adolescents and young adults from Spain.

Prioritising conservation actions towards the sustainability of the dehesa by integrating the demands of society

CONTEXTThe dehesa is an important agrosilvopastoral system in the Mediterranean, consisting of trees, livestock and pasture. It is a multifunctional system that offers a wide range of products and services, including food and forest products, as well as environmental and social services. However, this ecosystem is vulnerable in Andalusia, southern Spain, due to its low economic profitability and environmental and social challenges. OBJECTIVETo contribute to the design of public policies towards greater sustainability of the Andalusian dehesa. For this purpose, different conservation actions/sub-actions (CAs/CSs), i.e. agroforestry and pastoral practices, were evaluated and prioritised according to their expected contribution to sustainability. METHODSA multi-criteria model based on the Analytical Hierarchy Process (AHP) is developed to prioritise the environmental, economic and social demands of Andalusian society. It also allows estimating the contribution of CAs/CSs to the different functions of the dehesa and to the demands of society. The model is assessed on the basis of a survey of society in the dehesa areas of Andalusia and the knowledge of dehesa experts. RESULTS AND CONCLUSIONSSociety prioritises the environmental dimension of the dehesa. Thus, among society's demands, the highest priorities are the production of typical and quality landscapes and the conservation of biodiversity. In terms of the functions of the dehesa, the social ones stand out for their contribution to sustainability, especially the preservation of cultural heritage. After the social functions, forest production (economic function) and biodiversity (environmental function) are the highest priority functions. For the CAs, soil and water conservation and the renewal of trees are the highest priorities for improving the sustainability of the dehesa. Finally, the most critical CSs have also been identified as those with the greatest potential to improve sustainability based on current practices. SIGNIFICANCEThe methodology/model proposed for the design of policies to improve the sustainability of the Andalusian dehesa allowed the demands of society to be integrated into the process, understanding sustainability to be associated with the general well-being of society. The methodology can serve as a guide for assessing the sustainability associated with management actions in other agricultural systems and/or regions. Furthermore, the results obtained can contribute to the design of public policies towards greater sustainability of the dehesa not only in Andalusia, but also in other regions. The results highlight the importance of considering the productive and non-productive functions of the dehesa as a whole when designing public policies towards sustainability.

Low impact of tuberculosis severity on wild boar body condition

Body condition (BC), is a measure to assess the health status of domestic and wild animals. When food resources are abundant, a decrease in BC may indicate an increase in the energetic expenditure due to the effects of growth, reproduction, or disease. BC impoverishment is one of the most common clinical effects of diseases progressing chronically, such as animal tuberculosis (TB) caused by bacteria belonging to the Mycobacterium tuberculosis complex. The Eurasian wild boar (Sus scrofa) is the main wild TB reservoir in the Mediterranean basin. The specific aims of this work were to assess the relationship between sex, age and TB severity altogether on the BC of wild boar. For this purpose, we used the kidney fat index (KFI), to assess the impact of TB progression on the BC of 1372 hunter-harvested free-ranging wild boar in seven populations in southern Spain. Surprisingly, TB had only slight effects on wild boar BC and individuals exhibiting severe TB showed greater BC than TB-free individuals. The age (adults had greater BC than juveniles) and sex (females had greater BC than males) were the main BC determinants in wild boar. Sampling population and season explained more BC variability than individual factors, suggesting that other external factors might play an important role in the BC, and probably on the impact of the disease on this wild reservoir. The low impact of TB on wild boar BC suggests that individuals with severe TB and good BC represent potential long-term super-shedders of this pathogen.

Monitoring for Anguillicoloides crassus, Anguillid herpesvirus 1, aquabirnavirus EVE and rhabdovirus EVEX in the European eel population of southern Spain.

European eel is critically endangered in Europe. Among other stressors, pathogens are well-known to harm eels' fitness. One hundred and eighty-two eels were captured in three Eel Management Units in Andalucía (SE Spain) and analysed for Anguillicoloides crassus, Anguillid herpesvirus 1 (AngHV1), the rhabdovirus Eel Virus European X (EVEX) and the aquabirnavirus Eel Virus European (EVE). A. crassus adults and preadults were isolated and morphometrically identified, and the eel swimbladders were artificially digested to count A. crassus larvae. Also, eel tissues were examined by PCRs for the presence of viruses. EVEX and EVE were not detected in any of the eels. The estimated prevalence (95% confidence limits) was 71 (64-78)% for A. crassus and 35 (28-42)% for AngHV-1, varying these prevalences significantly between and within EMUs. Moreover, A. crassus prevalence was highest in smaller eels, in sites closest to the sea and eels sampled in the autumn. By contrast, AngHV-1 prevalence was highest in biggest eels, in sites far from the sea and sampled in the summer or winter. However, in mixed effects logistic models including site as a random variable, the risk of infection was associated with distance to the sea in both A. crassus and AngHV-1 infections and also to winter sampling in the case of AngHV-1 and not to other variables. These results are evidence that both pathogens are highly endemic in eels from Andalusian habitats. Further studies are needed to better understand the risk factors associated with these pathogens on eel populations.

Genomic Analysis of West Nile Virus Lineage 1 Detected in Mosquitoes during the 2020-2021 Outbreaks in Andalusia, Spain.

Emerging infectious diseases are one of the most important global health challenges because of their impact on human and animal health. The vector-borne West Nile virus (WNV) is transmitted between birds by mosquitos, but it can also infect humans and horses causing disease. The local circulation of WNV in Spain has been known for decades, and since 2010, there have been regular outbreaks in horses, although only six cases were reported in humans until 2019. In 2020, Spain experienced a major outbreak with 77 human cases, which was followed by 6 additional cases in 2021, most of them in the Andalusian region (southern Spain). This study aimed to characterize the genomes of the WNV circulating in wild-trapped mosquitoes during 2020 and 2021 in Andalusia. We sequenced the WNV consensus genome from two mosquito pools and carried out the phylogenetic analyses. We also compared the obtained genomes with those sequenced from human samples obtained during the outbreak and the genomes obtained previously in Spain from birds (2007 and 2017), mosquitoes (2008) and horses (2010) to better understand the eco-epidemiology of WNV in Spain. As expected, the WNV genomes recovered from mosquito pools in 2020 were closely related to those recovered from humans of the same outbreak. In addition, the strain of WNV circulating in 2021 was highly related to the WNV strain that caused the 2020 outbreak, suggesting that WNV is overwintering in the area. Consequently, future outbreaks of the same strain may occur in in the future.

Does type of accommodation influence tourist behavior? Hotel accommodation vs. rural accommodation

ABSTRACT Knowing how tourists behave in destinations is a key issue for successful marketing and management. Continuous changes in motivations of tourists highlight the necessity to understand visitors’ consumption patterns. The choices they make regarding the type of accommodation not only reflect tourists’ expectations, but also influence their behavior in the destination. This research aim is analyzing the possible differences in behavior between tourists depending on their preferred accommodation. The study was carried out in the province of Jaén, an emerging destination in southern Spain. A semi-structured survey was conducted with 430 visitors obtaining information that highlights, among other issues, the interest expressed by rural accommodation users in natural environments, along with more evenly disbursed spending patterns across the territory.

The bryophyte vegetation of gypsum outcrops: a focus on communities with Tortula revolvens

This paper describes a bryosociological study on the gypsicolous bryophyte communities of the Mediterranean area and central Europe, characterized by Tortula revolvens, the only bryophyte that is strictly gypsophytic. The cluster analysis has emphasized the occurrence of four associations belonging to the class Psoretea decipientis, well differentiated as concerns the ecological requirements and floristic composition; they are: Tortuletum revolventis and Trichostomo crispuli-Tortuletum revolventis of the order Barbuletalia unguiculatae, Crossidio crassinervis-Tortuletum obtusatae and Trichostomopso-Tortuletum obutasatae of the order Tortulo brevissimae-Aloinetalia bifrontis. Trichostomo crispuli-Tortuletum revolventis is here described as new. Considering the ecological requirements, type of gypsum outcrop and topographic location, it is possible to identify three distribution models: a first model with distribution in dry areas of central Europe and in Sicily, a second one with core distribution in the driest areas of southern Spain, and a third one in the semidesert and desert areas of Tunisia, Jordan and Israel.

Plant Traits and Phylogeny Predict Soil Carbon and Nutrient Cycling in Mediterranean Mixed Forests

Soil functioning is closely linked to the interactions between biological communities with the physical environment. Yet, the impact of plant community attributes on metabolic processes promoting soil nutrient cycling remains largely unknown. We hypothesized that the plant community acts as a regulating agent of nutrient mobilization in soils according to the phylogenetic and morpho-functional traits of plant species of which it is composed. Rhizosphere soils were collected in autumn and spring under 32 tree and shrub species in two Mediterranean mixed forests (four plots in each) located in southern Spain, and nine soil enzymatic activities related to C, N and P mobilization were assessed. Phylogeny and morpho-functional traits of plant species were recorded and their imprint in soil enzymatic activities across forests was determined. The results showed a plant phylogenetic signal for N mobilization in both forests, while it varied across forests for non-labile C and P mobilization. The plant phylogenetic signals were primarily driven by lineages that diversified through the Miocene, about 25 Myr ago. In addition, leaf traits and plant’s mycorrhizal type explained soil enzymatic activities independently from phylogeny. C and P mobilization increased under ectomycorrhizal plants, whilst enhanced N mobilization did occur under arbuscular mycorrhizal ones. The plant community composition led to a different carbon and nutrient mobilization degree, which in turn was mediated by distinct microbial communities mirroring differentiated resource-acquisition strategies of plants. Our results highlight the role of plant traits and mycorrhizal interactions in modulating carbon and nutrient cycling in Mediterranean mixed forest soils.

Harmonious Passion at Work: Personal Resource for Coping with the Negative Relationship between Burnout and Intrinsic Job Satisfaction in Service Employees.

Research about harmonious passion as a personal resource that can have a protective effect in situations of stress and burnout is scarce but growing. Considering the job demands-resources (JD-R) model, the present study aims to address the above gaps by exploring the moderating role of harmonious passion at work in the relationship between burnout (physical fatigue, cognitive weariness, and emotional exhaustion) and intrinsic job satisfaction. The study sample consisted of 748 workers from service organizations (front-line employees) in southern Spain (Mage = 35.51, SD = 10.06, 52% women). Using statistical program R (R Core Team, 2022), the results of the regression models showed the moderating role of harmonious passion on the negative relationships between physical fatigue and intrinsic job satisfaction. In particular, at high levels of physical fatigue, employees with high scores on harmonious passion at work presented higher levels of intrinsic satisfaction compared with employees with low passion at work. That is, although service employees have high levels of physical fatigue, when they are passionate, they still possess satisfaction with their work. Therefore, our findings extend the JD-R theory by considering harmonious passion as a motivational resource that reduce feelings of burnout in service employees. Hence, it shows the importance of promoting the autonomous internalization of work, (through, i.e., job enrichment), which allows the development of harmonious passion at work and, therefore, increasing intrinsic job satisfaction.

Evidence for parallel development of ever-growing molars in Early Pleistocene rodents from southern Spain and their paleoenvironmental implications

Evidence for parallel development of ever-growing molars in Early Pleistocene rodents from southern Spain and their paleoenvironmental implications

Egyptian and Oriental Ornaments in the Necropolis of Iulia Traducta (Algeciras, Strait of Gibraltar). Reflections on the Socio-Cultural Influences in the “Circle of the Strait” During the Early Roman Empire

Three small items belonging to different grave goods found in the Roman necropolis of Iulia Traducta (Algeciras, southern Spain) are analysed in this paper. Their clear provenance from Eastern Mediterranean contexts, as well as their characteristics, allow us to make some observations on the influence and continuity of Punic cultural, economic, and social traditions in this city at the start of the Roman Early Imperial period. The survival of certain Punic features in the face of Roman cultural currents has already been observed and reported by other researchers in various locations in the Strait of Gibraltar.

Perennial plant recovery after the removal of invasive Pinus halepensis in coastal habitats in Cádiz, southern Spain

Perennial plant recovery after the removal of invasive Pinus halepensis in coastal habitats in Cádiz, southern Spain