Source Of Sugars Research Articles

Sugar types, genetic predictors of the gut microbiome, and the risk of chronic kidney disease: a prospective cohort study.

Background: Emerging studies suggest that focusing on the intake of specific types or sources of sugars may yield greater benefits in preventing chronic kidney disease (CKD). Objective: We aimed to investigate the associations between free and non-free sugar intakes and CKD risk as well as the potential sugar type-gut microbiome interactions. Methods: A total of 138 064 participants from the UK Biobank were included in this prospective study. The free and non-free sugar intakes were assessed using repeated web-based 24-hour dietary recalls. A cause-specific competing risk model was used to estimate hazard ratios (HRs) and the corresponding confidence intervals (CIs) of incident CKD, treating deaths before incident CKD as competing events. Results: During a median follow-up of 10.5 years, 2,923 participants (2.1%) developed CKD. The free sugar intake was positively associated with the risk of CKD (HRquartile 4 vs. quartile 1 = 1.32, 95% CI = 1.18, 1.47), with a nonlinear relationship (P for nonlinearity = 0.01, the risk increased rapidly after free sugars made up 10% of the total energy). The non-free sugar intake was inversely associated with CKD risk (HRquartile 4 vs. quartile 1 = 0.68, 95% CI = 0.60, 0.77), with an L-shaped nonlinear curve (p for nonlinearity = 0.01, the turning point was at 13.5% of the total energy). We found that the associations between free sugar and non-free sugar intakes and CKD risk were more pronounced in participants with high genetically predicted gut microbial abundance. Furthermore, a significant interaction was observed between the genetically predicted gut microbial abundance and non-free sugar intake (P for interaction = 0.04). Conclusion: A higher intake of free sugars was associated with an elevated risk of CKD, whereas a higher intake of non-free sugars was associated with a reduced risk of CKD. The impact of free sugar intake and non-free sugar intake may be modified by the gut microbial abundance.

Odorant receptors for floral- and plant-derived volatiles in the yellow fever mosquito, Aedes aegypti (Diptera: Culicidae).

Adult mosquitoes require regular sugar meals, including nectar, to survive in natural habitats. Both males and females locate potential sugar sources using sensory proteins called odorant receptors (ORs) activated by plant volatiles to orient toward flowers or honeydew. The yellow fever mosquito, Aedes aegypti (Linnaeus, 1762), possesses a large gene family of ORs, many of which are likely to detect floral odors. In this study, we have uncovered ligand-receptor pairings for a suite of Aedes aegypti ORs using a panel of environmentally relevant, plant-derived volatile chemicals and a heterologous expression system. Our results support the hypothesis that these odors mediate sensory responses to floral odors in the mosquito's central nervous system, thereby influencing appetitive or aversive behaviors. Further, these ORs are well conserved in other mosquitoes, suggesting they function similarly in diverse species. This information can be used to assess mosquito foraging behavior and develop novel control strategies, especially those that incorporate mosquito bait-and-kill technologies.

Developing Biocontrol Strategies for the Management of Set rot Disease of Sugarcane under Wider Row Planting

Sugarcane (Saccharum officinarum L.) is one of the economically valuable agricultural crops grown worldwide in tropical and subtropical areas mainly for their sugar source. Among the 110 sugarcane cultivated countries, India and Brazil contribute half of global production [1]. During the period of last five decades the production, productivity and sugarcane recovery has also shown remarkable resilience in productivity growth rate. One of the reasons for the low productivity and recovery is increasing incidence of insect pests and diseases. An ecologically friendly alternative to these problems is biological control using rhizobacteria and their metabolic products [2]. In order to develop a technology capsule Surveys and surveillance were conducted in sugarcane fields both in plant and ratoon crops distributed in Orthanadu and Vallam block in New Cauvery delta area of Thanjavur dictrict during three consecutive years 2019- 22 with an objective to assess the incidence of sett rot, red rot and smut in New Cauvery delta area of Thanjavur dictrict. Fortnightly fixed plot observation was taken. The Maximium and minimum temperature, RH and quantity of rain and rainy days also recorded daily and consolidated on monthly basis and weather parameters were correlated with the incidence of disease. The results revealed that the maximum incidence sett rot negatively correlated with temperature. Similarly the number of rainy days and rainfall is positively correlated (r = 0.7523) with incidence of sett rot. For managing sett rot disease of sugarcane through biological approach, studies were conducted on biocontrol agents viz., Trichoderma asperellum (TNAU), Bacillus subtilis (TNAU), Chaetomium globosum (Cg6) with fungicide check propiconozole 0.1%. Among the treatment, highest millable cane (91,080/ha) and yield (91.39 tonnes / ha) and sugar yield ( 10.63 tonnes/ha) were recorded in M1- (Single bud treated with Trichoderma asperellum (TNAU)@ 4 g l-1 + soil application @ 2.5 kg ha-1) followed by M2- (Single bud treated with Bacillus subtilis (TNAU)@ 10 g l-1 + soil application @ 2.5 kg ha-1) in which, the cane yield of 88.54 tonnes/ha and sugar yield of 10.68 tonnes/ha were recorded. This study provides a technology capsule for the management of important disease of sugarcane.

Mushrooms for enhanced agriculture sustainability – the MUSA concept

The project MUSA – MUshrooms for Sustainable Agriculture is an effort to use mushroom-based processes to enhance agriculture sustainability in Nordic and Baltic countries. The project covers both the production of fruitbodies of edible fungi and the upgrading of the exhausted substrate from mushroom cultivation. The suitability of residues generated locally for producing edible mushrooms is investigated. Residues from Nordic agriculture and sub-utilized streams from forestry management, as well as wood processing by-products, are evaluated as the substrate base for producing shiitake (Lentinula edodes) and oyster (Pleurotus spp.) mushrooms. The project explores the potential of spent mushroom substrate (SMS) to support food production. SMS prospective as source of bioactive compounds and sugars is evaluated. MUSA investigates the suitability of SMS hydrolysates as carbon sources for cultivating oleaginous yeast to produce microbial oil suitable for human consumption. Using SMS for substituting mineral fertilizers and providing wastewater bioremediation solutions is also assessed.

Kelayakan Usaha Gula Kelapa Di Kabupaten Kulonprogo

The coconut plant (Cocos nucifera L.) is a strategic commodity that has an important role in the social, cultural and economic aspects of society. Kulon Progo Regency has a coconut plantation area of ​​16,104 ha, with productivity reaching 26,152 tons. Meanwhile, Panjatan District contributed 3,856 tons of coconut production with a coconut plantation area of ​​2,065 ha (BPS Kulon Progo Regency, 2021). The large area and high productivity of coconut in Panjantan District have great potential in developing the coconut sugar agro-industry. Apart from that, coconut sugar business actors in Kulon Progo Regency have received the European standard CSQA (Certified Software Quality Analyst) geographical indication certificate from the Ministry of Law and Human Rights. Coconut sugar has an important role in meeting local sugar needs, especially for people who still rely on natural sugar as a source of sugar. Coconut sugar also has high economic value, is in demand in local and international markets. So, this research wants to see the feasibility of the brown sugar business in Panjatan. The location determination technique was carried out purposively, while sampling was carried out using simple random sampling. Data was collected for one week. The results of the analysis show that the feasibility of the coconut sugar business in Panjatan is worth pursuing, because the results of the R/C value analysis are more than 1, namely 1.02. Capital productivity of 1.93% is greater than the bank savings interest rate of 0.175%. Labor productivity of Rp. 112,729,- exceeds the daily wage in Luweng Lor Village, namely Rp. 40,000,-. The BEP price is Rp. 13,106,- which is smaller than the average price for the coconut sugar household industry in Luweng Lor Village, which is Rp. 13,313,-. Meanwhile, the production BEP of 42 kg is smaller than the average production amount for coconut sugar craftsmen, namely 43 kg. To increase the coconut sugar business by using a larger tool for boiling sap, so that productivity will increase.

Pola Konsumsi Makanan Sumber Gula, Garam, Lemak dan Hubungannya dengan Penyakit Obesitas di Desa Babakan

Infectious diseases related to daily food consumption in Indonesia are increasing. Snack is type of food which contains lot of sugar, salt, an fat, can potentially cause many health problems, one of which is obesity. The prevalence of obesity in Babakan Village, Dramaga Bogor, West Java is 34.4%, with an age range of 18–85 years. This study aims to determine the relationship between consumption patterns of food sources of sugar, salt, and fat and obesity in adults in Babakan Village. This research uses quantitative descriptive methods and cross sectional study. This research was conducted through interviews with questionnaires, 24h recall, and FFQ. This research was conducted in August in Babakan Village, Dramaga District, Bogor, West Java. The results of this study show that there is no significant relationship between consumption patterns of food sources of sugar, salt, and fat and obesity in Babakan Village (p>0.05). The absence of a relationship between consumption of sugar, salt, and fat and the incidence of obesity is thought to be due to the predominance of respondents with normal nutritional status and malnutrition who consume more food sources of sugar, salt, and fat compared to those with obese nutritional status.

In vitro intestinal simulation system on the carbon source utilization characteristics and mechanism of interspecific syntrophic effects of Bifidobacterium longum CECT7894 and Pediococcus pentosaceus CECT8330.

The combination of Bifidobacterium longum and Pediococcus pentosaceus is a clinically effective probiotic formulation for alleviating infantile colic; however, their utilization characteristics and mechanism of action surrounding their combined use of sugar sources remains unclear. Using in vitro simulation technology, this study set up individual and mixed cultures of the two probiotics at unique concentrations, and different types of prebiotics, carbohydrates and polyols were added. Gas and short-chain fatty acid production, substrate utilization, as well as growth of the individual and mixed probiotics were detected at the beginning of fermentation, 24 h, and 48 h. Further, the mechanism of the syntrophic effect of the two probiotics was explored based on their growth characteristics. It was found that neither strain produced gas after 24 h and 48 h of cultivation, but could synergistically utilize fructo oligosaccharides (FOS) when mixed. There was an increasing trend of acetic acid production for B. longum in yeast extract, casitone and fatty acid (YCFA) and FOS medium with increasing of bacterial concentrations at 24 h and 48 h; whereas the trend for P. pentosaceus was less obvious. When bacterial concentrations were >5 billion CFU·g-1, the mixed culture showed significantly lower acetic acid production than B. longum alone. By adding lactic and acetic acids to the YCFA medium and observing P. pentosaceus growth, the results suggested that Pediococcus pentosaceus could use the acetic acid and lactic acid produced by Bifidobacterium longum for growth. When the bacterial concentration was 5 billion CFU·g-1, the acetic acid production of B. longum was significantly higher in the mixed cultures in lactulose, lactose, FOS, galactooligosaccharide, and inulin medium; whereas the reverse was true for culturing in xylitol, carboxymethyl cellulose sodium, and sorbitol medium. Further, the mixed cultures produced significantly more acetic acid than B. longum alone. In summary, through in vitro simulation experiments, the optimal ratio and potential interaction mechanisms between B. longum and P. pentosaceus were revealed here, offers a basis for understanding how the probiotic combinations may improve infant colic symptoms by influencing the gut pH and regulating the gut microbiota mechanisms.

Integration of mRNA and miRNA analysis reveals the molecular mechanisms of sugar beet (Beta vulgaris L.) response to salt stress

The continuous increase of saline-alkali areas worldwide has led to the emergence of saline-alkali conditions, which are the primary abiotic stress or hindering the growth of plants. Beet is among the main sources of sugar, and its yield and sugar content are notably affected by saline-alkali stress. Despite sugar beet being known as a salt-tolerant crop, there are few studies on the mechanisms underlying its salt tolerance, and previous studies have mainly delineated the crop’s response to stress induced by NaCl. Recently, advancements in miRNA-mRNA network analysis have led to an increased understanding of how plants, including sugar beet, respond to stress. In this study, seedlings of beet variety "N98122" were grown in the laboratory using hydroponics culture and were exposed to salt stress at 40 days of growth. According to the phenotypic adaptation of the seedlings' leaves from a state of turgidity to wilting and then back to turgidity before and after exposure, 18 different time points were selected to collect samples for analysis. Subsequently, based on the data of real-time quantitative PCR (qRT-PCR) of salt-responsive genes, the samples collected at the 0, 2.5, 7.5, and 16 h time points were subjected to further analysis with experimental materials. Next, mRNA-seq data led to the identification of 8455 differentially expressed mRNAs (DEMs) under exposure to salt stress. In addition, miRNA-seq based investigation retrieved 3558 miRNAs under exposure to salt stress, encompassing 887 known miRNAs belonging to 783 families and 2,671 novel miRNAs. With the integrated analysis of miRNA-mRNA network, 57 miRNA-target gene pairs were obtained, consisting of 55 DEMIs and 57 DEMs. Afterwards, we determined the pivotal involvement of aldh2b7, thic, and δ-oat genes in the response of sugar beet to the effect of salt stress. Subsequently, we identified the miRNAs novel-m035-5p and novel-m0365-5p regulating the aldh gene and miRNA novel-m0979-3p regulating the thic gene. The findings of miRNA and mRNA expression were validated by qRT-PCR.

Valorization of beverage waste as a sugar source for 5-hydroxymethylfurfural production

This study highlights the valorization of beverage waste as a promising sugar-based raw material for 5-hydroxymethylfurfural (5-HMF) production. In this work, both beverage waste purification and 5-HMF production were experimentally demonstrated through the practicality and effectiveness of continuous-flow processes. In the purification process, solid and liquid impurities were wholly eliminated through membrane filtration and a two-stage adsorption process using activated carbon. The purified beverage waste primarily consisted of fructose, glucose, and sucrose, offering a plentiful source of sugar. A mini fixed-bed reactor was employed to thoroughly investigate the impact of various operating variables on continuous 5-HMF production from synthetic beverage waste. The variables considered included residence time, reaction temperature, catalyst type, solvent type, water content, and sugar concentration. The optimization analysis was conducted to achieve both high 5-HMF yield and productivity. The optimal condition, which resulted in the 5-HMF yield of 42.6% and productivity of 3.3e−3 kg5-HMF/kgcat-h, was obtained with a residence time of 15 min, sugar feed concentration of 10 g/L, reaction temperature of 120 °C, and water content of 10 g/L. Dimethyl sulfoxide (DMSO) and Amberlyst 15 were the selected solvent and catalyst, respectively. The feasibility of producing 5-HMF from actual beverage waste was validated under optimal conditions. Furthermore, the stability of the catalyst was demonstrated over 24 h of time-on-stream. The proposed processes offer the potential for continuous purification of beverage waste and the efficient production of 5-HMF.

Combining bio- and chemo-catalysis for cyclic carbonate synthesis at very low pressure: Efficient “online” utilization of CO2 obtained directly from the fermentation of sugars

A very low-pressure set-up for the synthesis of cyclic carbonates and polycarbonates from epoxides/CO2 has been constructed using our previously reported Ga-catalyst. This set-up is based on the direct use of unpurified CO2 obtained from the fermentation of sugars, which exemplifies a potentially important advancement for the future application of this widely explored CO2 utilization reaction. Using this approach, it has been possible to produce cyclic carbonates with a high bio-derived atom content when starting from bio-based epoxides, such as, eugenol oxide. Use of alternative sources of sugar beyond sucrose have also been exemplified (grapes), highlighting the potential for direct coupling to important industrial fermentation processes which emit large amounts of CO2. Finally, use of 13C6-glucose as sugar-source has allowed for the preparation of 13C-containing cyclic carbonates, with the bis-cyclic carbonates being readily converted through reaction with a diamine to form 13C-labelled polymers (13C-Non-Isocyanate PolyUrethanes; 13C-NIPU’s).

High-temperature glycosylation of saccharides to modify molecular conformation of egg white protein and its effect on the stability of high internal phase emulsions

This paper investigates the freeze–thaw stability of oil-in-water emulsions stabilized by high-temperature wet heating glycosylation products. Glucose (Glu), D-fructose (Fru), xylose (Xyl), maltodextrin (MD), oligofructose (FO), and oligomeric isomaltulose (IMO) were chosen as sugar sources for the glycosylation reaction with egg white proteins (EWPs) at 120 °C to prepare the GEWPs. The study reveals that the type of sugar significantly influences the Maillard reactions with EWPs. The degree of glycosylation was highest in the Xyl group with the greatest reducing capacity and lowest in the MD, FO, and IMO groups. High-temperature wet glycosylation treatment induced changes in the secondary and tertiary structures of EWP. Elevated temperature exposed hydrophobic groups within the protein, while covalent binding of hydrophilic carbohydrates via the Maillard reaction decreased the protein's H0 value. Improved foaming and emulsifying properties were attributed to the increase in α-helix content, disulfide bond formation, and reduced surface tension. Emulsions prepared from GEWPs exhibited higher apparent viscosity and G' compared to those from natural EWPs, with the GEWP/Xyl group showing the highest values. After freeze–thaw treatment, the GEWP/Fru and GEWP/FO groups demonstrated superior stability and reduced freezing point, along with minimal microstructural alterations. These findings underscore the importance of sugar type in the stability of high internal phase emulsions (HIPEs) stabilized by GEWPs, indicating that a tailored Maillard reaction can yield stabilizers with exceptional freeze–thaw stability for emulsions.

FEATURES OF THE INTERACTION OF ROTARY CLEANERS WITH THE HEADS OF SUGAR BEET ROOTS

Sugar beets are one of the most important technical crops and one of the sources of sugar in Ukraine. Despite significant reductions in cultivated areas in recent years, sugar beets remain the main raw material for the sugar industry of Ukraine. In recent years, there has been a trend towards the use of foreign machinery for harvesting sugar beets, which was developed according to European quality standards of raw materials with increased sugar-bearing mass waste when the stalks are separated. Regardless of whether the technological process is carried out according to Ukrainian standards with minimal sugar-bearing mass waste, or according to European standards, with increased sugar-bearing mass waste, the task of determining the location of the sugar-bearing mass in space and its maximum separation remains in both cases. The task of harvesting sugar beets is to obtain the maximum possible amount of sugar-bearing mass from a unit of area with technologically and economically justified waste of sugar-bearing mass and the permissible content of physical and harmful pollutants - humus and soil. One of the important operations, which significantly affects the quality of raw materials, is the separation of chaff. Rotary working bodies with flexible and rigid with radially fixed elements are the most effective at separating the scum and are simple in design. However, they are effective at speeds of forward movement of beet harvesting machines no more than 1.5 m/s. The purpose of the work is to substantiate the process and develop new technical solutions for high-quality separation of the sugar-bearing mass with minimal sugar-bearing mass waste, which will increase the harvesting productivity. The article proposes the design of a root vegetable head cleaner with directed action of cleaning elements. A kinematic analysis and graphically modeled process of interaction of working elements with root crop heads for the serial and proposed root crop head cleaner were carried out. As a result of the research, the reason for the low-efficiency operation of serial cleaners at speeds of translational movement of beet harvesting machines more than 1.5 m/s was revealed, and the constructive and technological parameters of the cleaner with the directed action of the working elements were substantiated.

Application of segmented analysis via multivariate curve resolution with alternating least squares to 1H-nuclear magnetic resonance spectroscopy to identify different sugar sources.

The different types of sugar employed in the food industry exhibit chemical similarity and are mostly dominated by sucrose. Owing to the sugar origin of and differences in production, the presence of certain minor organic compounds differs. To differentiate between sugars based on their botanical source, geographical origin, or storage conditions, commercial brown sugars and sugar beet extracts were analyzed by 1H NMR spectroscopy applying a segmented analysis by means of multivariate curve resolution-alternating least squares (MCR-ALS). Principal component analysis and partial least squares-discriminant analysis yielded excellent differentiation between sugars from different sources after the application of this preprocessing strategy; without loss of chemical information and with direct interpretation of the results. By applying a segmented analysis via MCR-ALS to 1H NMR sugar data, similar spectroscopic profiles could be differentiated. This improved the selectivity of 1H NMR spectroscopy for sugar source differentiation which can be useful for industrial sugar authentication purposes.

A Review of the Diverse Genes and Molecules Involved in Sucrose Metabolism and Innovative Approaches to Improve Sucrose Content in Sugarcane

Sugarcane (Saccharum spp. hybrid) is the chief source of sugar and biofuel globally and is prominent among cash crops. Sucrose is the main required product in sugarcane, and many studies have been performed to understand the phenomena of sucrose synthesis, metabolism, and accumulation in sugarcane. However, none of the studies concluded that a single gene is responsible for the sucrose content. Instead, a complex mechanism consisting of several genes, such as sucrose phosphate synthase genes (SPS1, SPS2, SPS4, SPS5), sucrose synthase genes (SuSy1, SuSy2, SuSy4), invertase genes (INV, CWIN, NIN1, CINV2), and phytohormone, trehalose, transcription factor (TF), protein kinase, and sugar transporter genes are working spatiotemporally in sugarcane. Currently, omics approaches like transcriptomics, proteomics, and metabolomics are also being used to explore the sugar metabolism in sugarcane, but integrated transcriptomic, proteomic, and metabolomic studies have been less reported. The results obtained from the integrated analysis of transcriptomics, proteomics, and metabolomics are more reliable because the strong gene expression, received in the form of abundant mRNA, does not guarantee the plentiful existence of associated proteins or their particular activity in the target cells or tissues, which discloses the restraint of single interpretation and stresses the significance of the integrated analysis of transcriptomics, proteomics, and metabolomics. This review highlights different genes and molecules contributing to sugar metabolism at different stages and the significance of omics approaches in explaining sucrose metabolism, especially sucrose accumulation in sugarcane. It is also a vital source of knowledge for sugarcane breeders, particularly associated with sucrose content improvement and bioethanol energy production.

Valorisation of chewing gum production waste in bioethanol production: a response surface methodology study

ABSTRACT Interest in fast-moving consumer goods (FMCG) is constantly growing all over the world. Chewing gum, which is in the group of pleasant and entertaining snacks preferred by people of all ages, especially children and young people, is one of the most consumed products in the food sector. Due to its remarkable production and the major components, chewing gum waste has the potential as an alternative sugar source for bioethanol production. To evaluate bioethanol production from chewing gum wastes, the sugar was first extracted, and directly fermented into ethanol under different conditions. Saccharomyces cerevisiae (fresh and dry) and Saccharomyces bayanus were used as the yeast. To determine the optimal fermentation medium, the response surface methodology (RSM) study was employed and the fermentation medium variables were tested such as agar, pepton and glucose (APG medium) concentrations. The APG medium in which the maximum bioethanol yield obtained was determined as 5 g/L agar, 10 g/L peptone and 5 g/L glucose. The highest ethanol yield under optimal APG medium was obtained as 9.1-4 g ethanol/L sugar after 72 h by using Saccharomyces cerevisiae (fresh). The obtained results showed that chewing gum wastes can be utilised a potential source for the bioethanol production.

Nutrient-Sensitising Drug Repurposing Screen Identifies Lomerizine As a Mitochondrial Metabolism Inhibitor in Chronic Myeloid Leukaemia

In chronic myeloid leukaemia (CML), the persistence of leukaemic stem cells (LSCs) following first line treatment with tyrosine kinase inhibitors (TKIs) such as imatinib, can lead to disease relapse. We have previously demonstrated that therapy-resistant CML LSCs rely on oxidative phosphorylation (OXPHOS) for survival and that targeting mitochondrial metabolism sensitises CML LSCs to imatinib treatment. However, current OXPHOS inhibitors have demonstrated limited efficacy, or are associated with adverse effects in clinical trials, highlighting that identification of clinically safe oxidative pathway inhibitors is warranted. To identify clinically applicable compounds that target cells dependent on mitochondrial metabolism, we used a nutrient-sensitised repurposing screening strategy. Upon replacement of glucose with galactose as the only sugar source in cell culture media, there is a shift in cellular metabolism towards OXPHOS (as cells are unable to effectively use galactose for glycolysis). Therefore, CML cells grown in galactose-containing media are more sensitive to inhibition of mitochondrial respiration than glucose-grown cells. Using this approach, we screened total of >1,100 unique and structurally diverse FDA-approved drugs in CML cells, cultured in the presence of 11mM glucose or 11mM galactose. This uncovered lomerizine dihydrochloride, a L-type Ca2+ channel blocker used as an anti-migraine drug, as a candidate compound effective against OXPHOS-dependent CML cells. Subsequently, patient derived CML CD34+ cells were shown to have increased ER mass (the main intracellular Ca2+ store) and an increase in mitochondrial Ca2+ levels compared with normal cells. Moreover, transcriptional analysis revealed that Ca2+ channel genes such as voltage-gated CACNA1D and receptor-activated TRPC6 are significantly upregulated in CML LSCs (CD34+CD38-) when compared with normal haematopoietic stem cells (HSCs), which was not reverted following imatinib treatment. Therefore, we hypothesised that the observed increase in mitochondrial metabolism in CML LSCs was dependent on CACNA1D and TRPC6 activity, and the increase in mitochondrial Ca2+ levels, which could be inhibited by lomerizine treatment. Notably, CRISPR-Cas9-mediated CACNA1D or TRPC6 knockout significantly reduced cytoplasmic and mitochondrial Ca2+ levels, and decreased cellular oxygen consumption rate, indicative of reduction in OXPHOS. Similarly, while imatinib treatment did not affect intracellular Ca2+ levels in primary CML CD34+ cells, lomerizine treatment significantly reduced cytoplasmic and mitochondrial Ca2+ levels, and mitochondrial respiration/OXPHOS. Applying liquid chromatography-mass spectrometry and isotope assisted metabolomics, we traced labelled nutrients (glucose and palmitate) in CD34+ cells. This revealed a significant reduction in activity of mitochondria-located Ca2+ dependent dehydrogenases and overall reduction in tricarboxylic acid (TCA) cycle activity in primary CML cells following lomerizine treatment. Of clinical relevance, using combination of cell surface markers, cell division trackers and functional assays, including long-term culture initiating cell (LTC-IC) assay, we demonstrate that lomerizine treatment, alone or in combination with imatinib, targeted CML LSCs when cultured in physiological medium in vitro, without affecting normal HSCs. Notably, combination treatment with imatinib and lomerizine reduced CML tumour burden, targeted CML LSCs and extended survival in xenotransplantation model of human CML. In summary, we reveal for the first time that Ca2+ regulation is disrupted in CML stem cells and influx via CACNA1D and TRPC6 is crucial for maintaining high mitochondrial Ca2+ levels and enhanced mitochondrial OXPHOS. This renders CML stem cells sensitive to lomerizine, an FDA-approved Ca2+ inhibitor identified following metabolism-specific drug-repurposing screening.

The Variability of Proximate Composition, Sugars, and Vitamin C in Natural, Organic, and Biodynamic, and Fermented Leaves of Fireweed (Chamerion angustifolium (L.) Holub (Onagraceae))

Functional foods and herbs are becoming more and more popular as a way to improve health and at the same time improve diet. One of these plants is fireweed, which is abundant in fibers, proteins, and vitamin C in addition to polyphenols and carotenoids. Limited study is being carried out and there is limited information available about how the solid-phase fermentation and different growth systems change the proximate composition, as well as quantities of vitamin C, and sugars in the fireweeds leaves. The experiment was conducted in 2022 on an organic farm (Jonava district, Lithuania). The objective of this research was to determinate the impact of various growing systems (naturally, organically, and biodynamically) and duration (24, 48, and 72 h) effect of solid-phase aerobic fermentation on the changes of fibers, ash, proteins, vitamin C, and sugars. The fibers were established using the Kjeldahl method. High-performance liquid chromatography was used for sugars and vitamin C identification. The study found significant differences in the effects of various growing systems and solid-phase fermentation on the quantitative composition of substances in the fireweed leaves. Based on the data available, it is recommended to consume fermented fireweed leaves that are organically grown and fermented for a period of 48 h as they are a good source of vitamin C and fibers. Biodynamically grown fireweed leaves are a good source of ash and proteins. Naturally grown fireweed leaves, which are not fermented, are a good source of sugars.

Potential of Bacillus subtilis as oxygen-removal agent for biohydrogen production by Clostridium acetobutylicum

Molasses are a source of fermentable sugars, the substrate utilized for this study, as it is a subproduct from sugarcane processing for sucrose recovery. These sugars can be metabolized for bioH2 production by bacterial strains, particularly strict-anaerobe Clostridium acetobutylicum (Clac) and its media preparation requires chemical of O2 removal and N2 flushing to promote anaerobiosis. Bacillus subtilis (Bsub) can deplete O2 from culture media and promotes anaerobiosis, which is an aspect that would benefit from further research. Clac was grown in an anaerobic medium and produced 269 mL of hydrogen at 72 h. By co-culturing Clac and Bsub, 502 mL of bioH2 were produced in a cysteine/N2 flushed medium, while 203 mL of bioH2 at 72 h were produced by aerobic co-culture. Additionally, in an untreated/aerobic conventional growth culture for Clac, no production of H2 was detected, reinforcing the utility of Bsub for anaerobiosis generation.

Bacterial community dynamics during distilled spirit fermentation: influence of mash recipes and fermentation processes.

Production of ethanol from sugars and yeast is an ancient, ostensibly simple process. The source of sugars varies depending on the desired product and can include fruits, vegetables, molasses, honey, or grains, among other things. The source of yeast can be natural in the case of spontaneous ferments, but dry yeast addition is typical for large-scale fermentations. While the polymicrobial nature of some alcoholic fermentations is appreciated (e.g., for wine), most grain-based ethanol producers view microbes, apart from the added yeast, as "contaminants" meant to be controlled in order to maximize efficiency of ethanol production per unit of sugar. Nonetheless, despite rigorous cleaning-in-place measures and cooking the mash, bacteria are routinely cultured from these fermentations. We now know that bacteria can contribute to fermentation efficiency on an industrial scale, yet nothing is known about the makeup and stability of microbial communities in distilled spirit fermentations. The work here establishes the roles of mash recipes and distillery practices in microbial community assembly and dynamics over the course of fermentation. This represents an important first step in appreciating the myriad roles of bacteria in the production of distilled spirits.

An investigation of the performance of polyamide membrane for the concentration of pomegranate and effect on the physical and sports health of patients with physical weakness: A novel study

The purpose of fruit juice concentration is not only to prevent microbiological spoilage and chemical changes but also to cater to health and sport-related considerations such as nutritional value and athletic performance. Concentrated fruit juice is commonly used as a source of natural sugars, vitamins, and minerals in sports beverages and recovery drinks. In this study, the use of reverse osmosis (RO) as a method for thickening peach juice offers advantages in terms of preserving the nutritional content of the juice. By avoiding chemical reactions and requiring less energy, RO helps to retain the beneficial compounds present in the fruit juice, which are essential for supporting overall health and providing hydration during physical activities. The incorporation of silica nanoparticles in the polymer membrane used for the RO system can have additional health benefits. Silica nanoparticles have been investigated for their potential in enhancing nutrient absorption and promoting cellular health. By utilizing these nanoparticles in the membrane, the concentration process not only achieves the desired thickness but also has the potential to enhance the bioavailability of the nutrients present in the peach juice. Furthermore, the optimization of the RO system using statistical methods and software contributes to the efficiency and effectiveness of the concentration process. This optimization ensures that the desired concentration and flux are achieved, which is crucial for maintaining the desired taste, texture, and nutritional composition of the final fruit juice product. The use of RO and the incorporation of silica nanoparticles in fruit juice concentration have implications for both health and sport, offering a means to preserve the nutritional value of the juice while catering to the needs of athletes and individuals engaged in physical activities. Fruit juice can provide hydration and carbohydrates for athletes, supporting their health and athletic performance.