Valves play a major role in rectifying the fluid flow through piezo-hydraulic pumps, which may be active or passive. Passive valves operate due to changes in the fluid pressure whereas active valves are operated through control signals. This paper presents a novel design of an active valve chamber assembly for a piezo-hydraulic pump. It is a novel design since none of the research publications has addressed the application of a Flexurally amplified piezoelectric actuator (FAPA) for valve actuation in active valve chamber assembly. The piezo-hydraulic pump design is done using solid edge design software. FAPA’s are used for pumping and valve actuation. Structural analysis with experimental verification for the valve chamber component is presented. The valve opening and the pumping displacement are mathematically modeled in MATLAB Simulink software. Also, mathematical simulations for pump flow rates at different actuation frequencies and voltage are discussed. The piezo-hydraulic pump components are fabricated and assembled with the actuators. Experimental investigations on the flow rate performance of the active valve piezo-hydraulic pump are carried out with changing frequency and voltage amplitude of actuators. The interpretation of results is done by comparing the experimental and simulation results. During experiments, the pump delivered a maximum flow rate of 4.5 ml min−1 at 140 V when actuated at 0.25 Hz actuation frequency.