Simple Sampling Research Articles

Rapid detection of ingested acetaminophen on face mask by ambient ionization tandem mass spectrometry

BackgroundA regular face mask is comprised of three layers for resisting moisture, filtration, and absorbing oral fluid, respectively. Since the polymers with different polarities are used to make the layers, a face mask can be used as a sampling tool to retain polar or non-polar chemical and biochemical substances in the exhaled breath. In this study, thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS), an ambient ionization mass spectrometric technique, was used to detect trace acetaminophen that were exhaled and retained on the surface of different layers in a face mask. ResultsWith probe sampling combined with TD-ESI/MS/MS, the acetaminophen ion signal can be detected at the mouth/nostril region of the face mask after taking the acetaminophen tablet. The experimental results were similar to previous studies for the detection of acetaminophen in blood over time using LC/MS/MS. In addition, the intensities of acetaminophen on different layers of the face mask could reveal the differing distributions of exhaled acetaminophen on each layer. To explore the distribution of acetaminophen on the face mask surface, multiple probes were used to collect samples from different locations of the face mask for analysis. The molecular mapping of acetaminophen on the face mask was rendered by scaling the analyte ion signal intensity based on a temperature color gradient. The cartography showed a higher acetaminophen ion signal distribution on the mouth and nostril regions than in other areas of the face mask. SignificanceOwing to the advantages of a simple, sensitive, and non-invasive sampling approach, drug monitoring could be potentially performed to provide useful information for anti-drug of precision medicine in the future.

Power Outage and Vandalism in Akwa Ibom State, Nigeria

There are prevalent cases of vandalism on the national grid which usually throws Akwa Ibom State and Nigeria into darkness due to power outages. Transmission Company of Nigeria (TCN) is facing serious challenges of vandalism on electricity facilities across the country as it affects power supply, a situation that has become a social problem affecting both the rich and the poor. This study was conducted to examine the cause of power outage in Akwa Ibom State, Nigeria. Participants were selected using multiple sampling techniques; this includes purposive, snow-ball and simple sampling techniques. Primary data were collected through interview and participant observation. Secondary data were collected from extant literature to make it more scientific. The study recommends that the government should provide a security network that would protect electricity facilities across the state. Government should prosecute any vandal caught sabotaging electricity facilities in the state.

Blood based metabolic markers of glioma from pre-diagnosis to surgery

Gliomas are highly complex and metabolically active brain tumors associated with poor prognosis. Recent reports have found altered levels of blood metabolites during early tumor development, suggesting that tumor development could be detected several years before clinical manifestation. In this study, we performed metabolite analyses of blood samples collected from healthy controls and future glioma patients, up to eight years before glioma diagnosis, and on the day of glioma surgery. We discovered that metabolites related to early glioma development were associated with an increased energy turnover, as highlighted by elevated levels of TCA-related metabolites such as fumarate, malate, lactate and pyruvate in pre-diagnostic cases. We also found that metabolites related to glioma progression at surgery were primarily high levels of amino acids and metabolites of amino acid catabolism, with elevated levels of 11 amino acids and two branched-chain alpha-ketoacids, ketoleucine and ketoisoleucine. High amino acid turnover in glioma tumor tissue is currently utilized for PET imaging, diagnosis and delineation of tumor margins. By examining blood-based metabolic progression patterns towards disease onset, we demonstrate that this high amino acid turnover is also detectable in a simple blood sample. These findings provide additional insight of metabolic alterations during glioma development and progression.

Pengaruh Persepsi Harga dan Kualitas Produk Terhadap Customer Satisfaction dan Keputusan Pembelian

The purpose of this study is that we know the analysis of the effect of price perception. Product quality for CustomerSatisfaction and consumer purchasing choices. The object of this research is Indomaret Yoyakarta. The subjects ofthis research are customers who have shopped at Indomaret. This research is quantitative research. The sample ofthis study is part of the population, namely 100 customers who have shopped at Indomaret Yoyakarta. Where thissample is determined by the Slovin formula. The sampling technique used was simple accidental sampling. Datacollection techniques were carried out using a questionnaire or questionnaire method. The data analysis methodused is a regression method determined by t-experiment and classical assumptions. The data that has been obtainedis processed using SPSS 21. The results of this study indicate that the Price Perception Variable has a significanteffect on customer satisfaction based on the t experiment with a potential of 9.488 with a significance of <0.05resulting in Ho being rejected and Ha accepted. Product quality has a significant effect on customer satisfactionbecause it is based on a t-test of 2,973 with a significance <0.05. This results in Ho being rejected and Ha accepted.The Customer Satisfaction variable has a significant effect on consumer purchasing decisions as evidenced by thet-test with a value of 4.574 with a significance <0.05 which makes Ho rejected and Ha accepted. The priceperception variable has a significant effect on consumer purchasing decisions as evidenced by the t-test of 2.127with a significance <0.05 which resulted in Ho being rejected while Ha was accepted. Product quality has asignificant effect on consumer purchasing decisions as evidenced by the t-test of 3.246 with a significance <0.05which makes Ho rejected while Ha is accepted.

Pengaruh Persepsi Profesi dan Kesadaran Etis Terhadap Komitmen Profesi Akuntan Publik

A number of codes of ethics regulate how members of the public accounting profession are expected to behave. The problem of ethical violations that occur in the auditor environment is one of the things that needs to be considered, because it causes public trust in the public accounting profession to decline. The purpose of the written research is to find out: (1) how professional perception influences the level of commitment of the public accounting profession. (2) How ethical awareness influences the dedication of the public accounting profession. The written research is a survey research by distributing questionnaires to the sample directly. Participants in the written research were all auditors working at the Surabaya Public Accounting Office. The sample selected was 40 auditors with a simple sampling technique. Multiple regression analysis was used in the data analysis technique. The conclusion of the written research shows: (1) The commitment of the public accounting profession is significantly and positively influenced by public perception. The auditor's ethical awareness and dedication to the public accounting profession are positively correlated. The implications of the written research are in improving the skills, dedication, and professionalism of auditors working in public accounting offices so that they can provide services expected by the general public. This can be achieved by increasing knowledge in this field, which will influence the perception of the auditor profession. This can be done through training and discussions on the field of auditing.

Robust van der Waals Metal Mask for Residue‐Free and All‐Solid 2D Material Engineering

AbstractThe van der Waals (vdW) contact, characterized by its bondless interactions, opens up exciting possibilities in cutting‐edge mask technology. It enables incredibly close proximity to samples at the atomic level while facilitating non‐destructive engineering. In this study, the concept of a vdW metal mask using the template striped ultra‐flat Ag/Au film is introduced. The probe tip‐assisted metal film transfer under an optical microscope is employed to showcase all‐solid and residue‐free engineering on 2D materials. The robust nature of the vdW metal mask allows for various treatments, including gas, liquid, solid, plasma, and light, making it a universal tool for fabricating 2D material‐based devices and samples with sub‐1 µm resolution, all without the need for lithography technologies. With the superiority in simple sample fabrication, ultra‐clean surfaces, and robustness under harsh conditions, the technique is believed to flourish in the 2D material research field.

Quantitative analysis of microplastics in beach sand via low-temperature solvent extraction and thermal degradation: Effects of particle size and sample depth

Quantifying trace levels of microplastics in complex environmental media remains a challenge. In this study, an approach combining field collection of samples from different depths, sample size fractionation, and plastic quantification via pyrolysis-gas chromatography–mass spectrometry (Py-GC–MS) was employed to identify and quantify microplastics at two public beaches along the northeast coast of the U.S. (Salisbury beach, MA and Hampton beach, NH). A simple sampling tool was used to collect beach sand from depth intervals of 0–5 cm and 5–10 cm, respectively. The samples were sieved to give three size fractions: coarse (>1.2 mm), intermediate (100 μm–1.2 mm), and fine (1.2 μm–100 μm) particles. Following density separation and wet peroxide oxidation, a low-temperature solvent extraction protocol involving 2-chlorophenol was used to extract polyester (PET), polystyrene (PS), polyamide (PA), and polyvinyl chloride (PVC). The extract was analyzed using Py-GC–MS for the respective polymers, while the solid residue was pyrolyzed separately for polyethylene (PE) and polypropylene (PP). The one-step solvent extraction method significantly simplified the sample matrix and improved the sensitivity of analysis. Among the samples, PET was detected in greater quantities in the fine fraction than in the intermediate size fraction, and PET fine particles were located predominantly in the surface sand. Similar to PET, PS was detected at higher mass concentrations in the fine particles in most samples. These results underscore the importance of beach environment for plastic fragmentation, where a combination of factors including UV irradiation, mechanical abrasion, and water exposure promote plastic breakdown. Surface accumulation of fine plastic particles may also be attributed to transport of microplastics through wind and tides. The proposed sample treatment and analysis methods may allow sensitive and quantitative measurements of size or depth-related distribution patterns of microplastics in complex environmental media.

A high throughput facility for the RF characterisation Of planar superconducting thin films

Abstract Accelerator laboratories worldwide are researching copper radio frequency (RF) cavities coated with superconducting thin films to exceed the limits of bulk niobium. The development and RF testing of thin films on small planar samples is vital before cavity depositions. A team at Daresbury Laboratory have developed a cost-effective facility using a novel 7.8 GHz Choke Cavity for the RF characterisation of planar samples. RF chokes ensure that no electrical contact is required between the sample and the cavity. The main advantages are: a simple sample design (90 − 130 mm diameter disk with no sample-cavity welding) and easy operation using a LHe-free cryostat. This enables high sample throughput, with up to 3 sample tests per week, making the facility suitable for quick, systematic scanning of deposition parameters. With the sample thermally and physically isolated from the test cavity, it is possible to measure the average surface resistance, R s, directly using an RF-DC compensation method. Facility commissioning has been performed with bulk and thin film niobium samples. These tests have demonstrated the ability to measure R s at temperatures in the range 4 − 20 K and sample peak magnetic fields up to 3 mT. The minimum resolvable R s is 0.5 μΩ with typical uncertainties of 9 − 15%. The design, operation and commissioning of this facility is reported in this paper.

Lessons learned: drive-through COVID-19 clinic testing during an adaptive epidemic response and a point-of-care test assessment of a computer-read rapid lateral flow immunoassay with fluorescence-based detection.

Background. The COVID-19 pandemic demonstrated a need for robust SARS-CoV-2 test evaluation infrastructure to underpin biosecurity and protect the population during a pandemic health emergency.Gap statement. The first generation of rapid antigen tests was less accurate than molecular methods due to their inherent sensitivity and specificity shortfalls, compounded by the consequences of self-testing. This created a need for more accurate point-of-care SARS-CoV-2 detection methods.Aim. Here we present the lessons-learned during the COVID-19 emergency response in Western Australia including the detailed set-up, evaluation and operation of rapid antigen test in a state-run drive-through sample collection service during the COVID-19 pandemic after the strict border shutdown ended.Methods. We report a conformity assessment of a novel, second-generation rapid antigen test (Virulizer) comprising a technician-operated rapid lateral flow immunoassay with fluorescence-based detection.Results. The Virulizer rapid antigen test demonstrated up to 100% sensitivity (95% CI: 61.0-100%), 91.94% specificity (95% CI: 82.5-96.5%) and 92.65% accuracy when compared to a commercial PCR assay method. Wide confidence intervals in our series reflect the limits of small sample size. Nevertheless, the Virulizer assay performance was well-suited to point-of-care screening for SARS-CoV-2 in a drive-through clinic setting.Conclusion. The adaptive evaluation process necessary under changing pandemic conditions enabled assessment of a simple sample collection and point-of-care testing process, and showed how this system could be rapidly deployed for SARS-CoV-2 testing, including to regional and remote settings.

Decentralized food safety and authentication on cellulose paper-based analytical platform: A review.

Food safety and authenticity analysis play a pivotal role in guaranteeing food quality, safeguarding public health, and upholding consumer trust. In recent years, significant social progress has presented fresh challenges in the realm of food analysis, underscoring the imperative requirement to devise innovative and expedient approaches for conducting on-site assessments. Consequently, cellulose paper-based devices (PADs) have come into the spotlight due to their characteristics of microchannels and inherent capillary action. This review summarizes the recent advances in cellulose PADs in various food products, comprising various fabrication strategies, detection methods such as mass spectrometry and multi-mode detection, sampling and processing considerations, as well as applications in screening food safety factors and assessing food authenticity developed in the past 3 years. According to the above studies, cellulose PADs face challenges such as limited sample processing, inadequate multiplexing capabilities, and the requirement for workflow integration, while emerging innovations, comprising the use of simplified sample pretreatment techniques, the integration of advanced nanomaterials, and advanced instruments such as portable mass spectrometer and the innovation of multimodal detection methods, offer potential solutions and are highlighted as promising directions. This review underscores the significant potential of cellulose PADs in facilitating decentralized, cost-effective, and simplified testing methodologies to maintain food safety standards. With the progression of interdisciplinary research, cellulose PADs are expected to become essential platforms for on-site food safety and authentication analysis, thereby significantly enhancing global food safety for consumers.

Consumers’ Perception of Online Shopping in Kota Samarahan, Sarawak

In contemporary times, individuals commonly employ a combination of conventional and modern approaches to get products and services, driven by shifts in consumer purchasing patterns. This is seen in the growing preference for cashless payment methods when making purchases. This study aims to examine the elements that impact customers' perception of online shopping in Kota Samarahan and assess the correlation between two variables that influence consumers' impression of online shopping. The sample approach employed for this investigation is random simple sampling. The data was obtained using a survey conducted in person, where participants scanned a QR code to access the questionnaire. A total of 208 respondents were included in this survey, and the data was analyzed using descriptive analysis and Pearson correlations. This study identified four factors that impact consumers' impression of online purchasing. The study identified four distinct characteristics that influenced customers' view of online buying: convenience, website design/feature, time efficiency, and security. These aspects were considered independent variables, while consumers' perception of online shopping was the dependent variable. All the results from the correlation coefficient data were determined to be positive, suggesting a direct association between the variables.

Baseline measurements in the assessment of ESS-specific radionuclide uptake by crops cultivated in Southern Sweden

The European Spallation Source, ESS, is a neutron research facility under construction in Lund, Southern Sweden. The Facility will produce neutrons by spallation, using a powerful linear accelerator to deliver protons to a tungsten target. In addition to the desired neutron production, a long list of radionuclides will be created as by-products of the nuclear reaction inside the target. The Swedish Radiation Safety Authority has established a list of the most relevant radionuclides, in terms of contribution to the effective dose to ESS workers and the general public, should an accidental release of irradiated target material occur. This list includes radionuclides that are not produced by the nuclear energy industry, in particular 178mHf, 182Ta, 187W, 148Gd and 173Lu. Ongoing research efforts aim to determine the best analytical methods to assess these exotic and often difficult-to-measure radionuclides in environmental samples. This work investigates the potential of X-ray fluorescence and time-of-flight elastic Recoil detection analysis for the assessment of soil samples, and the potential of particle induced X-ray emission for the assessment of crop samples. These techniques require only simple sample preparation steps and no chemical extraction, unlike the conventional environmental monitoring methods such as inductively-coupled plasma mass spectrometry, and show promise as complimentary methods enabling fast sample throughput. This study focuses on the analysis of uncontaminated soil and crops, to provide baseline data, whilst simultaneously assessing the available measurement capabilities. For the X-ray fluorescence system used in this study, the method detection limit for W in soil was determined to be 0.147ppth, and Zr which can be correlated with the migration of Hf was clearly measurable.

Contextual Acceptance of COVID-19 Mitigation Mobile Apps in the United States: Mixed Methods Survey Study on Postpandemic Data Privacy.

The COVID-19 pandemic gave rise to countless user-facing mobile apps to help fight the pandemic ("COVID-19 mitigation apps"). These apps have been at the center of data privacy discussions because they collect, use, and even retain sensitive personal data from their users (eg, medical records and location data). The US government ended its COVID-19 emergency declaration in May 2023, marking a unique time to comprehensively investigate how data privacy impacted people's acceptance of various COVID-19 mitigation apps deployed throughout the pandemic. This research aims to provide insights into health data privacy regarding COVID-19 mitigation apps and policy recommendations for future deployment of public health mobile apps through the lens of data privacy. This research explores people's contextual acceptance of different types of COVID-19 mitigation apps by applying the privacy framework of contextual integrity. Specifically, this research seeks to identify the factors that impact people's acceptance of data sharing and data retention practices in various social contexts. A mixed methods web-based survey study was conducted by recruiting a simple US representative sample (N=674) on Prolific in February 2023. The survey includes a total of 60 vignette scenarios representing realistic social contexts that COVID-19 mitigation apps could be used. Each survey respondent answered questions about their acceptance of 10 randomly selected scenarios. Three contextual integrity parameters (attribute, recipient, and transmission principle) and respondents' basic demographics are controlled as independent variables. Regression analysis was performed to determine the factors impacting people's acceptance of initial data sharing and data retention practices via these apps. Qualitative data from the survey were analyzed to support the statistical results. Many contextual integrity parameter values, pairwise combinations of contextual integrity parameter values, and some demographic features of respondents have a significant impact on their acceptance of using COVID-19 mitigation apps in various social contexts. Respondents' acceptance of data retention practices diverged from their acceptance of initial data sharing practices in some scenarios. This study showed that people's acceptance of using various COVID-19 mitigation apps depends on specific social contexts, including the type of data (attribute), the recipients of the data (recipient), and the purpose of data use (transmission principle). Such acceptance may differ between the initial data sharing and data retention practices, even in the same context. Study findings generated rich implications for future pandemic mitigation apps and the broader public health mobile apps regarding data privacy and deployment considerations.

Rapid analysis of spilled petroleum oils by direct analysis in real time time-of-flight mass spectrometry with hydrophobic paper sample collection

BackgroundOil spills are widespread and can cause devastating environmental consequences. Rapid oil identification is critical to find the origin of the spill, monitor the environment, and lead to informed mitigation measures. The current standard methods in oil spill identification are precise and reliable, but require extensive sample preparation, long instrument runs, and time-consuming data processing. Direct analysis in real time time-of-flight mass spectrometry (DART-ToF MS) has been employed to screen for spilled petroleum oils, with results obtained in mere hours. The present study introduced an innovative, simple, and fast oil sampling method using hydrophobic filter paper and demonstrated its compatibility with DART-ToF MS analysis. Motor oils, jet fuels, marine diesels, crude oils, intermediate fuel oils, heavy fuel oils, and diluted bitumen were collected using the filter paper sampling method. Classification models were constructed from the spectral data by heat map inspection followed by principal component analysis (PCA) and discriminant analysis of principal components (DAPC). Oil slicks and weathered oil slicks were prepared from five oil types, and samples from each slick were collected using filter paper.ResultsThe filter paper technique allowed for effective oil sampling and data acquisition by DART-ToF MS for diluted source oils, oil slicks and weathered oil slicks. Classification via the constructed DAPC models indicated that the DART-ToF MS instrument in tandem with filter paper sampling and multivariate statistics can accurately identify common oil types, with significant improvement of sample collection and turnaround time.ConclusionsThe promising classification results, simple sample collection, and rapid data analysis illustrate the potential use of hydrophobic filter paper and DART-ToF MS as tools in managing large scale oil spill emergency situations.

Assemble and validation of a simulated device of human exhaled breath aerosols for screening of illicit drug abuse by HPLC-TQ-MS/MS detection

Collecting exhaled breath aerosols (EBA) as a sample has a great potential to monitoring illicit drug abuse due to advantages of simple, non-invasiveness and unrestricted sampling. However, scarcity of positive samples has cramped development of more efficient techniques for monitoring illicit drug abuse in EBA. In this work, a simulated human exhaled breath aerosol (SHEBA) generating device based on Venturi jet principle was constructed by integrating nebulizing unit and collection unit to prepare positive EBAs. By means of ultra high-performance hyphenated with triple quadrupole tandem mass spectrometry (HPLC-TQ-MS/MS), using caffeine as a target drug, the device parameters were investigated by studying the effects of flow rates of nebulizing and auxiliary gases, drug concentration and etc., the performance was further validated by commonly abused drugs such as amphetamines (AMP, MAMP, MDA and MDMA). Electret material as adsorbent was used to collect SHEBAs generated by the device, achieving HPLC-TQ-MS/MS detection of the captured drugs at 40.0–2000.0 pg/filter. By this way, caffeine content in EBAs from six volunteers was successfully detected in the range of 51.6 ± 6.8–708 ± 51 pg/filter after drinking the caffeinated beverage. These validation experiments proved that SHEBA generating device could not only meet to the requirement of EBA analysis for illicit drug abuse at trace levels, but also provide the correction factors to calibrate the lower detection value caused by incomplete material adsorption.

Assessing Urban Neighbourhood Resilience to Covid 19 Pandemic in Jos, Plateau State, Nigeria

The sudden incidence of COVID-19 at Wuhan in China in December 2019 with its significant death globally and other negative socio-economic impacts has awakened the need to make human settlements resilient to unforeseen tragedy. Nigeria and Jos the capital of Plateau State have had negative experiences with the incidence of covid 19. Considering this, the study aims at assessing the resilience of urban neighbourhoods in Jos North Local Government Area of Plateau State to COVID-19 pandemic. The study objective is to examine the pattern and characteristics of some selected neighborhoods within Jos the study area. In addition to examining the characterisation of infrastructural facilities and to evaluate the environmental challenges that impede adherence to non-pharmaceutical protocols to COVID-19. Two neighbourhoods, Angwan Rukuba and Fudawa were selected to represent the entire Jos North Local Government Area. Relevant literature related to the study was reviewed. The study adopted a direct observation method of the physical characteristics of the neighborhoods and a simple sampling technique of survey where a structured questionnaire was administered to 138 respondents in the two neighborhoods. Descriptive analysis using percentages and tables in relation to the data obtained was used to draw inferences and conclusions. The results obtained shows that the sampled neighborhoods are densely congested informal settlements with unorganized settlement pattern, lacking in basic neighbourhoods’ infrastructure, and poor circulation, among others. These findings imply that the environmental setting of the study area makes compliance with non-pharmaceutical protocols during COVID-19. pandemic difficult. By implication, the neighborhoods lack the resilience capability to cope with the COVID-19. pandemic. The study recommends the need for planning and upgrading of the neighbourhoods’ facilities along with stakeholder public enlightenment on the need to comply to COVID-19. non-pharmaceutical protocols as a step toward building resilient neighbourhoods in Jos North Local Government Area and the metropolis at large.

PENGARUH MOTIVASI KERJA, DISIPLIN KERJA DAN BEBAN KERJA TERHADAP KINERJA PEGAWAI HARIAN LEPAS MELALUI KEPUASAN KERJA SEBAGAI VARIABEL INTERVENING PADA POLRES SITUBONDO

The aim of this study is to find out the impact of work motivation, work discipline and workload on the performance of post-day officers through job satisfaction as an intervening variable on Situbondo POLRES. The population on this study is the Officer of the Post-POLRES Situbondo Daily. Simple probability sampling is a method of sample-taking without considering the population in this study. Data analysis and hypothesis testing in research using Structural-partial equation model Least square (PLS-SEM). The results of the hypothesis test of its direct influence using the smart PLS3.2.8 application, show that work motivation has a significant positive effect on job satisfaction, work discipline has a significant positive effect on job satisfaction, workload has a positive but not significant effect on job satisfaction, work motivation has a significant positive effect on performance, work discipline has a significant positive effect on performance, workload has a positive but not significant effect on performance, job satisfaction has a positive but not significant effect on performance, work motivation has a positive but not significant effect on performance through job satisfaction, work discipline has a positive but not significant effect on performance through job satisfaction, workload has a positive but not significant effect on performance through job satisfaction.

Practices of Management Accounting Techniques in Service Sector Public Enterprises in Nepal

Management accounting is the act of making sense of financial and costing data and translating that data into useful information for management and officers within an organization. This research aims at examining the practice of management accounting and the major difficulties in applying management accounting tools in the public enterprises of the service sector of Nepal. It also tries to assess the impact of management accounting system on the performance of sampled organizations. The specific survey research has been followed with a descriptive-analytical approach. A structured questionnaire was used to collect primary data from the respondents which were analyzed by the multiple correlation and regression model. The study employed simple judgmental sampling procedure to draw the samples. Out of the seven public enterprises in Nepal that operate in the service sector five were selected as the samples. The results revealed that there is a significant impact of management accounting systems on organizational performance. The study suggested to implement the effective managerial tools and techniques a separate managerial accounting department should be established within an organization. The study also recommended that academicians who have better knowledge about MA tools and techniques should put in the effort to develop environmentally friendly tools and techniques into practice and also to keep management accounting information system. It helps to keep them informed from time to time about every aspect of MA and its application.

Rapid Determination of 12 Classes of Per- and Polyfluoroalkyl Substances in Water Samples from Environmental Forensic Cases.

The widespread use of per- and polyfluoroalkyl substances (PFASs) with different physico-chemical properties poses a great threat to the environment and human health. Simultaneous detection of different classes of PFASs is a difficult task, especially for rapid analysis of polluted water samples in environmental forensic cases. In this study, a simple sample preparation ultrahigh-performance liquid chromatography coupled with quadrupole Orbitrap high-resolution mass spectrometry was established for the detection of PFASs in a wide range of water matrices. By optimizing the conditions of pretreatment and the parameters of the instrument, the developed method provided good linearity of calibration standards (R2 > 0.99), and demonstrated excellent MLOQ (0.008-1.2 µg/L), with spiked recoveries ranging from 57.7% to 151% for 47 targets in surface water samples, and from 45.7 to 165% for 46 targets in ground and waste water samples, respectively. This method required an injection volume of 3 µL and an analysis time of only 18 min per sample. The validation method was successfully applied to the analysis of 20 environmental water samples, in which 15 target substances with different concentrations were detected, with total concentrations of 0.082 to 262.455 μg/L. The method is simple and exclusive, and can rapidly confirm the occurrence of PFASs in different water samples, providing a convenient and fast high-throughput analysis, which is especially suitable for the application in the environmental forensic investigation of PFASs pollution.

PENGARUH KEPEMIMPINAN TRANSFORMASIONAL, LINGKUNGAN KERJA DAN MOTIVASI KERJA TERHADAP KINERJA ASN DENGAN DIMEDIASI KESIAPAN UNTUK BERUBAH PADA UPT PUSKESMAS JANGKAR

This study aims to determine the impact of transformational leadership, work environment, and motivation on ASN performance by mediating readiness to change on UPT Puskesmas Jangkar. The population in this study is ASN on UPT Puskesmas Jangkar, which is a simple probability sampling method that does not consider the population in this study. The sampling uses saturated samplings, which are sample determination techniques when all members of the population are sampled. Data analysis and hypothesis testing in research using Structural-partial equation model Least square (PLS-SEM). The results of the hypothesis are tested directly using the PLS 3.0 smart application. Demonstrate that transformational leadership has a positive but non- significant influence on the willingness to change, and the working environment has a significant positive impact on the readiness to change. Transformational leadership has a significant positive impact on performance, the working environment has a positive but non-significant impact on Performance, and the motivation for work has a negative but not significant effect on performance. Preparedness to change has a positive but insignificant impact on performance. Transformational leadership has a positive but non-significant impact on Performance through Preparedness for Change, a working environment with a positive yet insignificant influence on Performance via Preparation to Change, and work motivation with a positive but not significant impact on Output through Preparation for Change.