Serial Sequence Research Articles

Identifying deviant sexual interest in a sex offender sample using dual-target rapid serial visual presentation task

To investigate the dual-target rapid serial visual presentation (dtRSVP) task as a measure of deviant sexual interest (i.e. a sexual interest in children), we administered a dtRSVP with gender- and age-specific pictorial stimuli to child sex offenders (n = 69), other sex offenders (n = 43), non-sex offenders (n = 14), and community controls (n = 88). We hypothesized that (1) stimuli belonging to the preferred gender and age group presented as targets (both T1 and the subsequent T2) in the serial sequence would be more accurately detected than non-preferred stimuli and that (2) this increased detection would reduce the detection of targets later in the serial sequence (T2) due to an attentional blink. Our findings supported hypothesis 1 and partly supported hypothesis 2. Although we found group differences, individual indices based on detection rates did not allow for individual-level diagnostic categorization of participants.

The cortisol awakening response is associated with performance of a serial sequence reaction time task

There is emerging evidence of a relationship between the cortisol awakening response (CAR) and the neural mechanisms underlying learning and memory. The aim of this study was to determine whether the CAR is associated with acquisition, retention and overnight consolidation or improvement of a serial sequence reaction time task. Salivary samples were collected at 0, 15, 30 and 45min after awakening in 39 healthy adults on 2 consecutive days. The serial sequence reaction time task was repeated each afternoon. Participants completed the perceived stress scale and provided salivary samples prior to testing for cortisol assessment. While the magnitude of the CAR (Z score) was not associated with either baseline performance or the timed improvement during task acquisition of the serial sequence task, a positive correlation was observed with reaction times during the stable performance phase on day 1 (r=0.373, p=0.019). Residuals derived from the relationship between baseline and stable phase reaction times on day 1 were used as a surrogate for the degree of learning: these residuals were also correlated with the CAR mean increase on day 1 (r=0.357, p=0.048). Task performance on day 2 was not associated with the CAR obtained on this same day. No association was observed between the perceived stress score, cortisol at testing or task performance. These data indicate that a smaller CAR in healthy adults is associated with a greater degree of learning and faster performance of a serial sequence reaction time task. These results support recognition of the CAR as an important factor contributing to cognitive performance throughout the day.

Electrically Driven Random Laser Memory

The first electrically driven random laser diode with nonvolatile resistive random access memory functionality is designed and demonstrated. To illustrate the working principle, a metal–insulator–semiconductor structure based on Pt/MgO/ZnO thin‐film layers is fabricated on indium tin oxide glass. The current–voltage curve of the dual‐function random laser memory (RLM) device exhibits an excellent electrical bistability with a high ON/OFF current ratio (≈107). The random lasing behavior is simultaneously demonstrated by using electrical pumping with the appearance of sharp‐peak emissions and a drastic enhancement of peak intensity. A wide angle‐dependent electroluminescence not only reveals its emitting advantage but also further supports the origin of random lasers. The first proof‐of‐concept presentation of RLM possesses several advantages of dual memory and lasing functions, which enables to open up new avenues to practical applications, such as light emitting memories for electrical and optical communication. This new horizon for the realization of all optical memories should therefore be able to attract academic as well as industrial interests. It is stressed here that the electrical reading of conventional memory array is usually in serial sequence, which limits the maximum data throughput. This hurdle can be overcome by optically readable memory devices.

Economic Dispatch With Non-Smooth Objectives—Part II: Dimensional Steepest Decline Method

Economic dispatch (ED) considering valve-point effect, multiple fuel options, prohibited operating zones of generation units is a more accurate model compared to a conventional ED model. It is non-smooth and thus evolutionary algorithms (EAs) are so far the only feasible approaches for the model. In Part II of the paper, a new method, the dimensional steepest decline method (DSD), is proposed for the ED with non-smooth objectives. The DSD is based on the local minimum analysis of the ED problem presented in Part I of the paper. The fuel cost's decline rate between each two adjacent singular points is utilized to find the optimal solutions in a serial sequence. The computational complexity of the DSD is analyzed. The DSD has been applied to solve different types of ED problems on different test systems, including large-scale systems. The simulation results show that DSD can obtain more accurate solutions and consume much less time and its advantage is more obvious on large-scale systems, in comparison with the state-of-art EAs.

Perpendicular serial maxillary distraction osteogenesis in cleft lip and palate patients.

Background:Cleft lip and palate patients often have a retruded maxilla with a severely narrowed deficient maxillary arch. This report aims to describe the management of severe maxillary retrusion and constriction in cleft lip and palate patients using distraction osteogenesis applied in serial sequence in two directions perpendicular to each other.Materials and Methods:Two adult male cleft lip and palate patients were treated with maxillary distraction osteogenesis in two stages. In the first stage, surgically assisted rapid palatal expansion with a tooth-borne device was performed to significantly expand the maxillary arch in the transverse dimension. After the teeth were orthodontically aligned, the horizontal distraction of the maxilla was made by two internal maxillary distraction devices.Results:In the first patient, the maxilla was initially widened by 11 mm and then distracted forward by 20 mm. Despite the breakage of the shaft of one of the two distractors at the end of distraction, a satisfactory occlusion was found at the time of distractor device removal. The maxillary position has remained stable through 8 years of follow-up. In the second patient, the palate was widened by 14 mm and the maxilla was distracted forward by 22 mm. The maxillary position has remained stable through 3 years of follow-up.Conclusion:Sequential serial distraction of maxilla in two planes perpendicular to each other is a safe and stable approach for the treatment of cleft lip and palate patients with severe transverse and anteroposterior discrepancies.

Digital immunohistochemistry platform for the staining variation monitoring based on integration of image and statistical analyses with laboratory information system.

BackgroundDigital immunohistochemistry (IHC) is one of the most promising applications brought by new generation image analysis (IA). While conventional IHC staining quality is monitored by semi-quantitative visual evaluation of tissue controls, IA may require more sensitive measurement. We designed an automated system to digitally monitor IHC multi-tissue controls, based on SQL-level integration of laboratory information system with image and statistical analysis tools.MethodsConsecutive sections of TMA containing 10 cores of breast cancer tissue were used as tissue controls in routine Ki67 IHC testing. Ventana slide label barcode ID was sent to the LIS to register the serial section sequence. The slides were stained and scanned (Aperio ScanScope XT), IA was performed by the Aperio/Leica Colocalization and Genie Classifier/Nuclear algorithms. SQL-based integration ensured automated statistical analysis of the IA data by the SAS Enterprise Guide project. Factor analysis and plot visualizations were performed to explore slide-to-slide variation of the Ki67 IHC staining results in the control tissue.ResultsSlide-to-slide intra-core IHC staining analysis revealed rather significant variation of the variables reflecting the sample size, while Brown and Blue Intensity were relatively stable. To further investigate this variation, the IA results from the 10 cores were aggregated to minimize tissue-related variance. Factor analysis revealed association between the variables reflecting the sample size detected by IA and Blue Intensity. Since the main feature to be extracted from the tissue controls was staining intensity, we further explored the variation of the intensity variables in the individual cores. MeanBrownBlue Intensity ((Brown+Blue)/2) and DiffBrownBlue Intensity (Brown-Blue) were introduced to better contrast the absolute intensity and the colour balance variation in each core; relevant factor scores were extracted. Finally, tissue-related factors of IHC staining variance were explored in the individual tissue cores.ConclusionsOur solution enabled to monitor staining of IHC multi-tissue controls by the means of IA, followed by automated statistical analysis, integrated into the laboratory workflow. We found that, even in consecutive serial tissue sections, tissue-related factors affected the IHC IA results; meanwhile, less intense blue counterstain was associated with less amount of tissue, detected by the IA tools.

A cognitive framework for explaining serial processing and sequence execution strategies.

Behavioral research has produced many task-specific cognitive models that do not say much about the underlying information-processing architecture. Such an architecture is badly needed to better understand how cognitive neuroscience can benefit from existing cognitive models. This problem is especially pertinent in the domain of sequential behavior where behavioral research suggests a diversity of cognitive processes, processing modes and representations. Inspired by decades of reaction time (RT) research with the Additive Factors Method, the Psychological Refractory Period paradigm, and the Discrete Sequence Production task, we propose the Cognitive framework for Sequential Motor Behavior (C-SMB). We argue that C-SMB accounts for cognitive models developed for a range of sequential motor tasks (like those proposed by Keele et al., Psychological Review, 110(2), 316-339, 2003; Rosenbaum et al., Journal of Experimental Psychology: Human Perception and Performance, 9(1), 86-102, 1983, Journal of Memory and Language, 25, 710-725, 1986, Psychological Review, 102, 28-67, 1995; Schmidt, Psychological Review, 82(4), 225-260, 1975; Sternberg et al., 1978, Phonetica, 45, 177-197, 1988). C-SMB postulates that sequence execution can be controlled by a central processor using central-symbolic representations, and also by a motor processor using sequence-specific motor representations. On the basis of this framework, we present a classification of the sequence execution strategies that helps researchers to better understand the cognitive and neural underpinnings of serial movement behavior.

Ego depletion impairs implicit learning.

Implicit skill learning occurs incidentally and without conscious awareness of what is learned. However, the rate and effectiveness of learning may still be affected by decreased availability of central processing resources. Dual-task experiments have generally found impairments in implicit learning, however, these studies have also shown that certain characteristics of the secondary task (e.g., timing) can complicate the interpretation of these results. To avoid this problem, the current experiments used a novel method to impose resource constraints prior to engaging in skill learning. Ego depletion theory states that humans possess a limited store of cognitive resources that, when depleted, results in deficits in self-regulation and cognitive control. In a first experiment, we used a standard ego depletion manipulation prior to performance of the Serial Interception Sequence Learning (SISL) task. Depleted participants exhibited poorer test performance than did non-depleted controls, indicating that reducing available executive resources may adversely affect implicit sequence learning, expression of sequence knowledge, or both. In a second experiment, depletion was administered either prior to or after training. Participants who reported higher levels of depletion before or after training again showed less sequence-specific knowledge on the post-training assessment. However, the results did not allow for clear separation of ego depletion effects on learning versus subsequent sequence-specific performance. These results indicate that performance on an implicitly learned sequence can be impaired by a reduction in executive resources, in spite of learning taking place outside of awareness and without conscious intent.

Differences between late preterm and full-term infants: comparing effects of a short bout of practice on early reaching behavior.

This study compared the effects of a short bout of practice on reaching behavior between late preterm and full-term infants at the onset of goal-directed reaching. Twelve late preterm infants and twelve full-term infants received reaching practice based on a serial schedule. Late preterm and full-term infants were assessed in 3.3±1.4 and 2.6±1.0 days after the onset of goal-directed reaching in two measures in a single day: immediately before practice (pre-test) and immediately after practice (post-test). During the assessments, the infants were placed in a baby chair and a rubber toy was presented at their midline within reaching distance for 2 min. Between assessments, the infants received practice of toy-oriented reaching in 3 activities repeated for approximately 4 min. The activities were elicited in a pre-established serial sequence and were applied by a physical therapist. During the pre-test, late preterm infants presented lower range of proximal adjustments, greater proportion of reaches with semi-open hand, and greater proportion of reaches without grasping than the full-term infants. During the post-test, late preterm infants presented greater motor variability of proximal adjustments, but explored and selected distal control and grasping outcomes less compared to the full-term group. Differences in reaching and gross motor behavior between late preterm and full-term infants can be found at the age of reaching onset. Practice provided new opportunities for late preterm infants to improve perception-action coupling to reach; however, relative to full-terms, they seemed less advanced in benefiting from the experience for more refined manual tasks.

Quantifying transfer after perceptual-motor sequence learning: how inflexible is implicit learning?

Studies of implicit perceptual-motor sequence learning have often shown learning to be inflexibly tied to the training conditions during learning. Since sequence learning is seen as a model task of skill acquisition, limits on the ability to transfer knowledge from the training context to a performance context indicates important constraints on skill learning approaches. Lack of transfer across contexts has been demonstrated by showing that when task elements are changed following training, this leads to a disruption in performance. These results have typically been taken as suggesting that the sequence knowledge relies on integrated representations across task elements (Abrahamse, Jiménez, Verwey, & Clegg, Psychon Bull Rev 17:603-623, 2010a). Using a relatively new sequence learning task, serial interception sequence learning, three experiments are reported that quantify this magnitude of performance disruption after selectively manipulating individual aspects of motor performance or perceptual information. In Experiment 1, selective disruption of the timing or order of sequential actions was examined using a novel response manipulandum that allowed for separate analysis of these two motor response components. In Experiments 2 and 3, transfer was examined after selective disruption of perceptual information that left the motor response sequence intact. All three experiments provided quantifiable estimates of partial transfer to novel contexts that suggest some level of information integration across task elements. However, the ability to identify quantifiable levels of successful transfer indicates that integration is not all-or-none and that measurement sensitivity is a key in understanding sequence knowledge representations.

Generation of Human Immunodeficiency Virus–1 Long Terminal Repeat Reporter Genes by Rapid Polymerase Chain Reaction–Mediated Mutagenesis

Objective: The development of a human immunodeficiency virus (HIV)–1 promoter reporter system is critical for investigating transcription activity of the virus. We designed a modified overlap extension PCR method for single round site-directed mutagenesis of the long terminal repeat (LTR) segment of HIV-1. Methods: The procedure consists of overlap extension PCR, DpnI digestion, and product transformation. Wild-type and serial sequence deletion clones of HIV-1 LTR were constructed. Basal transcription activity was also measured. Results: The overall efficiency for obtaining the desired products was 53.2%. The reporter assay indicated the importance of the second NF-ĸB cis-element and surrounding regions in mediating the regulation of HIV-1 transcription. Conclusion: The technique we investigated is suitable for highthroughput functional studies of virus-host interaction.

Implicit perceptual-motor skill learning in mild cognitive impairment and Parkinson’s disease.

Implicit skill learning is hypothesized to depend on nondeclarative memory that operates independent of the medial temporal lobe (MTL) memory system and instead depends on cortico striatal circuits between the basal ganglia and cortical areas supporting motor function and planning. Research with the Serial Reaction Time (SRT) task suggests that patients with memory disorders due to MTL damage exhibit normal implicit sequence learning. However, reports of intact learning rely on observations of no group differences, leading to speculation as to whether implicit sequence learning is fully intact in these patients. Patients with Parkinson's disease (PD) often exhibit impaired sequence learning, but this impairment is not universally observed. Implicit perceptual-motor sequence learning was examined using the Serial Interception Sequence Learning (SISL) task in patients with amnestic Mild Cognitive Impairment (MCI; n = 11) and patients with PD (n = 15). Sequence learning in SISL is resistant to explicit learning and individually adapted task difficulty controls for baseline performance differences. Patients with MCI exhibited robust sequence learning, equivalent to healthy older adults (n = 20), supporting the hypothesis that the MTL does not contribute to learning in this task. In contrast, the majority of patients with PD exhibited no sequence-specific learning in spite of matched overall task performance. Two patients with PD exhibited performance indicative of an explicit compensatory strategy suggesting that impaired implicit learning may lead to greater reliance on explicit memory in some individuals. The differences in learning between patient groups provides strong evidence in favor of implicit sequence learning depending solely on intact basal ganglia function with no contribution from the MTL memory system.

The processing of emotional stimuli during periods of limited attentional resources in schizophrenia.

When participants are asked to attend to two target stimuli in a rapid serial visual presentation sequence, the successful identification of the 1st target (T1) leads to transient impairment in reporting the 2nd target (T2)--this effect is known as the attentional blink (AB). In healthy individuals, this AB effect is either diminished or accentuated when emotional stimuli are presented in the T2 or T1 positions, respectively, suggesting that affective content influences bottom-up (i.e., exogenous) attention. In the current study, we conducted two separate experiments using the Emotional Attentional Blink paradigm where emotional words were presented in the T2 or T1 position to determine whether schizophrenia patients with high and low negative symptoms differ from controls in the extent to which emotional stimuli influence bottom-up attentional processes. Participants included 33 schizophrenia patients and 28 controls in Experiment 1 (T2 Task), and 30 schizophrenia patients and 24 controls in Experiment 2 (T1 Task). In both experiments, patients were divided into high (HI-NEG) and low (LOW-NEG) negative symptom subgroups using the Scale for the Assessment of Negative Symptoms. Results of Experiment 1 indicated that controls and LOW-NEG patients displayed the typical pattern of AB sparing at early lags for emotional relative to neutral words; however, HI-NEG patients showed no difference in T2 accuracy between emotional and neutral stimuli. Results of Experiment 2 indicated that controls and LOW-NEG patients displayed reduced T2 accuracy following unpleasant T1 stimuli, while HI-NEG patients showed no decrement in T2 accuracy after emotional T1s. Across both experiments, findings suggest that emotional stimuli have a bottom-up competitive advantage in LOW-NEG patients and controls; however, this bottom-up advantage is absent in HI-NEG patients.

Subclonal evolution involving SF3B1 mutations in chronic lymphocytic leukemia

Subclonal evolution involving SF3B1 mutations in chronic lymphocytic leukemia

Peat Water Treatment Using Combination of Cationic Surfactant Modified Zeolite, Granular Activated Carbon, and Limestone

This research was conducted essentially to treat fresh peat water using a series of adsorbents. Initially, the characterization of peat water was determined and five parameters, including pH, colour, COD, turbidity, and iron ion exhibited values that exceeded the water standard limit. There were two factors influencing the adsorption capacity such as pH, and adsorbent dosages that were observed in the batch study. The results obtained indicated that the majority of the adsorbents were very efficient in removing colour, COD, turbidity at pH range 2-4 and Fe at pH range 6-8. The optimum dosage of cationic surfactant modified zeolite (CSMZ) was found around 2 g while granular activated carbon (GAC) was exhibited at 2.5 g. In column study, serial sequence of CSMZ, GAC, and limestone showed that the optimal reduction on the 48 hours treatment were found pH = 7.78, colour = 12 TCU, turbidity = 0.23 NTU, COD = 0 mg/L, and Fe= 0.11 mg/L. Freundlich isotherm model was obtained for the best description on the adsorption mechanisms of all adsorbents.

Reconstructing the Dynamics of HIV Evolution within Hosts from Serial Deep Sequence Data

At the early stage of infection, human immunodeficiency virus (HIV)-1 predominantly uses the CCR5 coreceptor for host cell entry. The subsequent emergence of HIV variants that use the CXCR4 coreceptor in roughly half of all infections is associated with an accelerated decline of CD4+ T-cells and rate of progression to AIDS. The presence of a ‘fitness valley’ separating CCR5- and CXCR4-using genotypes is postulated to be a biological determinant of whether the HIV coreceptor switch occurs. Using phylogenetic methods to reconstruct the evolutionary dynamics of HIV within hosts enables us to discriminate between competing models of this process. We have developed a phylogenetic pipeline for the molecular clock analysis, ancestral reconstruction, and visualization of deep sequence data. These data were generated by next-generation sequencing of HIV RNA extracted from longitudinal serum samples (median 7 time points) from 8 untreated subjects with chronic HIV infections (Amsterdam Cohort Studies on HIV-1 infection and AIDS). We used the known dates of sampling to directly estimate rates of evolution and to map ancestral mutations to a reconstructed timeline in units of days. HIV coreceptor usage was predicted from reconstructed ancestral sequences using the geno2pheno algorithm. We determined that the first mutations contributing to CXCR4 use emerged about 16 (per subject range 4 to 30) months before the earliest predicted CXCR4-using ancestor, which preceded the first positive cell-based assay of CXCR4 usage by 10 (range 5 to 25) months. CXCR4 usage arose in multiple lineages within 5 of 8 subjects, and ancestral lineages following alternate mutational pathways before going extinct were common. We observed highly patient-specific distributions and time-scales of mutation accumulation, implying that the role of a fitness valley is contingent on the genotype of the transmitted variant.

Parallel Programming Methods Based on the Multi-Core DSP TMS320C6670

While the frame rate is higher and the image size is larger, sequence images processing is harder. Good real-time can be ensured by the multi-core DSP in the embedded image processing system. TMS320C6670 which is the multi-core DSP designed by TI corporation is selected as study object. Based on hardware characteristics analyzed, the Data Flow model is adopted as the multi-core processing model. Two data processing subtasks assigning methods are analyzed by comparing their advantages and disadvantages on the system idle time and memory requirements. The data processing subtask assigning flow is design for a serial sequence images processing example. An inter-core data transfer flow design idea is put forward. Using methods and occasion of two kinds of data buffer establishing techniques is studied and defined. An inter-core notification flow design idea is put forward. Using methods and occasion of three notification methods based on the interrupt controller and the Semaphore2 module is studied and defined.

Operating characteristics of the implicit learning system supporting serial interception sequence learning.

The memory system that supports implicit perceptual-motor sequence learning relies on brain regions that operate separately from the explicit, medial temporal lobe memory system. The implicit learning system therefore likely has distinct operating characteristics and information processing constraints. To attempt to identify the limits of the implicit sequence learning mechanism, participants performed the serial interception sequence learning (SISL) task with covertly embedded repeating sequences that were much longer than most previous studies: ranging from 30 to 60 (Experiment 1) and 60 to 90 (Experiment 2) items in length. Robust sequence-specific learning was observed for sequences up to 80 items in length, extending the known capacity of implicit sequence learning. In Experiment 3, 12-item repeating sequences were embedded among increasing amounts of irrelevant nonrepeating sequences (from 20 to 80% of training trials). Despite high levels of irrelevant trials, learning occurred across conditions. A comparison of learning rates across all three experiments found a surprising degree of constancy in the rate of learning regardless of sequence length or embedded noise. Sequence learning appears to be constant with the logarithm of the number of sequence repetitions practiced during training. The consistency in learning rate across experiments and conditions implies that the mechanisms supporting implicit sequence learning are not capacity-constrained by very long sequences nor adversely affected by high rates of irrelevant sequences during training.

Position–item associations play a role in the acquisition of order knowledge in an implicit serial reaction time task.

Knowledge of sequential regularities plays a key role in forms of explicit and implicit memory, such as working memory and motor skills. Despite important advances in the study of sequence knowledge in the past century, the theoretical development of implicit and explicit memory has occurred separately. Unlike the literature on implicit sequence learning, the explicit learning literature differentiates between 2 forms of representation of serial structure, chaining (C is the item following B in the sequence A-B-C-D) and ordinal position knowledge (C is the 3rd item). In 3 experiments, we demonstrate that these 2 forms of sequence knowledge can be acquired in implicit sequence learning. In Experiment 1, 2 trained sequences were recombined at transfer such that the strength of (a) associations between serial positions and sequence elements as well as (b) associations between successive sequence elements could be estimated. In Experiment 2, we compared sequence elements placed at the trained versus untrained serial position. Experiment 3 reduced cues that can be used to determine the start of a sequence within the stream of trials. Our results suggest that the discussion held in explicit memory research about different forms of representation of sequences knowledge also is relevant for implicit sequence learning.

Molecular biology of oncogenic inflammatory processes. I. Non-oncogenic and oncogenic pathogens, intrinsic inflammatory reactions without pathogens, and microRNA/DNA interactions (Review)

In some inflammasomes tumor cells are generated. The internal environment of the inflammasome is conducive to the induction of malignant transformation. Epigenetic changes initiate this process. The subverted stromal connective tissue cells act to promote and sustain the process of malignant trans-formation. In its early stages, the premalignant cells depend on paracrine circuitries for the reception of growth factors. The ligands are derived from the connective tissue, and the receptors are expressed on the recipient premalignant cells. The initial events are not a direct attack on the proto-oncogenes, and thus it may be entirely reversible. Epigenetic processes of hypermethylation of the genes at the promoters of tumor suppressor genes (to silence them), and deacetylation of the histones aimed at the promoters of proto-oncogenes (to activate them) are on-going. A large number of short RNA sequences (interfering, micro-, short hairpin, non-coding RNAs) silence tumor suppressor genes, by neutralizing their mRNAs. In a serial sequence oncogenes undergo amplifications, point-mutations, translocations and fusions. In its earliest stage, the process is reversible by demethylation of the silenced suppressor gene promoters (to reactivate them), or re-acetylation of the histones of the oncogene promoters, thus de-activating them. The external administration of histone deacetylase inhibitors usually leads to the restoration of histone acetylation. In time, the uncorrected processes solidify into constitutive and irreversible gene mutations. Some of the pathogens inducing inflammations with consquential malignant transformation contain oncogenic gene sequences (papilloma viruses, Epstein-Barr virus, Kaposi's sarcoma-associated herpesvirus, hepatitisB and C viruses, Merkel cell polyoma virus, Helicobacter pylori, enterotoxigenic Bacteroides fragilis). These induced malignancies may be multifocal. Other pathogens are devoid of any known oncogenic genomic sequences (mycoplasma vav-carcinogenesis, chlamydia MALT-lymphoma genesis). In these cases the host's inflammatory reactions induce the malignant transformation in serial sequences of gene alterations initiated by hypoxia and reactive oxygen and nitrogen species generation. Carcinogenic intrinsic inflammatory processes endogenously initiated without a pathogen are recognized. Chronic inflammatory processes signal the RNA/DNA complex. In response, the DNA may revert into its ancient primordial 'immortal' format, which the clinics recognize as 'oncogenesis'. The DNA remains the ultimate master of bioengineering in order to sustain life. A discussion on the most versatile and resistant primordial RNA/DNA complex and the pre-, proto-, and unicellular world in which they co-existed is included.