Papaya Seeds Research Articles

Diversity Profiling of Seed Associated Endophytic Microbiome in Important Species of Caricaceae Family

Background: Plant associated endophytic microbes play an important role in plant’s growth and development. After seed germination, the seed associated endophytes rapidly colonize the seedlings and help in their growth and protection against pathogens. This study was aimed to understand the diversity in the endophytic microbial population associated with the seeds of papaya (Carica papaya) and its wild relatives from Vasconcellea genus (family: Caricaceae). The species of Vasconcellea genus are widely used to introgress virus resistance in cultivated varieties of papaya. Hence, the diversity of seed associated endophytic microbes and their gene functional analysis was carried out through next generation sequencing of the microbial 16S rRNA and ITS sequences. Results: The 16S rRNA amplicon analysis revealed that the number of operational taxonomic units (OTUs) was higher for the endophytic bacteria, ranging between 144–204 when compared to 41–69 OTUs for the endophytic fungi. The bacterial phylum Proteobacteria was the most abundant seed associated phylum, with 64.7–72.8% abundance, across all four species of Caricaceae family, followed by Firmicutes (13.6–26.1%), Patescibacteria (1.1–2%) and Actinobacteria (0.7–2.7%). With respect to the diversity of bacteria by abundance indices, Vasconcellea goudotiana had the highest OTUs of 204, followed by 177 in V. cauliflora, 156 in V. cundinamarcensis, and 144 in C. papaya. The alpha diversity indices and functional analysis revealed the differences in the OTUs and the functional annotations among the above four plant species. The fungal OTUs were in the range of 41–69; however, only a small fraction of them could be taxonomically classified. Conclusion: Our microbiome studies reveal the differences in the seed associated endophytic microbial community across the four plant species of Caricaceae family. This study also unravels the composition of endophytic microbial population associated with the seeds of different plant species of Caricaceae family and their gene functions. It also provides an insight into both culturable and nonculturable endophytic microbes. Further this study reveals that domestication of Carica papaya might have resulted into reduced microbial diversity when compared to their wild relatives from Vasconcellea genus.

Siamese network‐based computer vision approach to detect papaya seed adulteration in black peppercorns

Food adulteration is a growing peril for consumers, traders, and manufacturers worldwide. Food fraud costs the economy a fortune and creates mistrust among consumers and merchants. Black Pepper is a valuable and heavily adulterated spice. This study explores the ability of deep learning coupled with image processing to identify black pepper contaminated with its common adulterant papaya seeds. Prevalent methods work on a relatively small sample, requiring specialization and resources. Our research proposes a system to quickly and economically obtain a method to trace added impurities. It is a problematic venture for one-shot image classification systems to differentiate between identical entities. Therefore, we implement a Siamese network that is proficient in it. A data set of 2,000 images of each class is created. After experimentation, the training and validation accuracy of 0.96 and 0.92 is obtained, while human eye judgment of the same images gave a correctness score of 0.97. Novelty impact statement A novel non-destructive, agile, cost-effective, user-friendly technique to trace papaya seeds' adulteration in black peppercorns using Artificial Intelligence. The proposed system is better in accuracy than the majority of existing approaches.

A study on the impact of tin concentration on microstructural, dielectric and conductivity properties of ITO nanoparticles

Indium tin oxide (ITO) nanoparticles synthesized by green combustion method using Carica papaya seed extract as a novel fuel and, indium (In) and tin (Sn) as precursor is presented in this paper. Indium nitrate and tin nitrate solution were prepared by dissolving the In ingots and Sn in nitric acid. Carica papaya seed extract was prepared using dried Carica papaya seeds. Carica papaya seed extract along with indium nitrate and tin nitrate solution was heated in muffle furnace at 600 °C for 1 h. The obtained powder was calcinated at 650 °C for 3 h to obtain ITO nanoparticles. The effect of Sn concentration (5%, 10% and 50%) on microstructure, dielectric and ac conductivity of ITO nanoparticles were studied. These properties were investigated using X-ray diffraction (XRD), scanning electron microscope (SEM), high resolution transmission electron microscope (HRTEM), UV–Vis spectroscopy, thermal gravimetry and differential scanning calorimetry (TG-DSC) and computer-controlled impedance analyser. The XRD analysis of synthesized ITO nanoparticles exhibits cubic bixbyite structure with increase in crystallite size from 17 nm to 22 nm as Sn concentration increases. SEM and HRTEM investigations exhibit an increase in grain size as Sn concentration increases. TG-DSC analysis reveals a constant weight in the temperature region 200 to 800 °C. The diffused reflectance spectral studies revealed that the band gap decreases from 3.01 eV to 2.75 eV as Sn concentration increases. The ac conductivity and dc conductivity of ITO nanoparticles was found to increase with increase in Sn concentration and temperature. With improved microstructural properties, higher optical band gap and increased ac conductivity, synthesized ITO nanoparticles with 10% Sn concentration are suitable material for sensor and optoelectronic applications.

Pengaruh Suhu dan Waktu Penyangraian Terhadap Aktivitas Antioksidan dan Sifat Sensoris Kopi Tiruan Biji Pepaya (Carica papaya L.)


 
 
 This Study aims to determine the effect of temperature and roasting time on antioxidant activity and sensory properties of artificial coffee papaya seeds to produce papaya seeds coffee with high antioxidant activity and the best sensory properties. The experimental design used in this study was a Factorial Completely Randomized Design which were the first factor is the roasting temperature (80oC, 90oC, 100oC) and the second factor is the roasting time (5 minutes, 10 minutes, 15 minutes). The treatment was repeated 2 times to obtain 18 experimental units. The data was analyzed with analysis of variance and if the treatment had a significant effect, then followed by Duncans Multiple Range Test. The parameters tested in this study included moisture content, total phenol, total flavonoid, qualitative caffeine, antioxidant activity and sensory properties. The results showed that papaya coffee seeds with a temperature of 100oC and roasting time of 10 minutes were the best treatment with water content value of 3.76%, total phenol 1.25 mgGAE/g, total flavonoids 0.29 mgQE/g, antioxidant activity 24.03%, caffeine (qualitative) negative with sensory properties color was yellowish-brown, aroma was distintive of papaya seeds, taste was bitter, and overall acceptance was liked.
 
 

The Effect of Young Papaya Seed Extract on the Number of Male Mice Spermatozoes (Mus musculus)

Papaya fruit has many benefits, one of which is papaya seed can be used as antifertility drugs especially young papaya seeds. This is because young papaya seeds contain secondary metabolites of alkaloid glycosides and proteolytic enzymes such as papain which can interfere with spermatogenesis. This aims to determine the effect of young papaya seed extract of a mice number spermatozoa. Mice were randomly selected to represent 4 dose groups: control group (P0), extract 30mg/BW (PI), extract 60mg/BW (PII) and extract 90mg/BW (PIII). Each treatment group was carried out with six repetitions. The treatment was given intravenously by injecting it in the tail of the mice for 15 consecutive days. The observed spermatozoa quality variables is a number of spermatozoa. Data analysis used one way ANOVA significance test 5% (p<0,05) and continued with the LSD (Least Significant Difference) 95% confidence. The result of the study on the quality of spermatozoa showed a significant effect at the doses of 60mg/BW (PII) and 90mg/BW (PIII). The higher dose of young papaya seed extract, it increasingly affects the number of sperm. The research concluded that the extract of young papaya seeds has a significant impact of a mice number spermatozoa (Mus musculus).

UJI AKTIVITAS ANTIBAKTERI ISOLAT SENYAWA GOLONGAN TERPENOID DARI BIJI PEPAYA (Carica papaya L.) TERHADAP Staphylococcus aureus SECARA IN VIVO PADA KELINCI JANTAN

research on the isolation of terpenoid class of compounds from the seeds of papaya (Carica papaya L.) and test its activity against Staphylococcus aureus in vivo in male rabbits. This research aims to prove that the terpenoid compounds isolated from the seeds of papaya (Carica papaya L.) can inhibit the growth of Staphylococcus aureus in vivo. Separation of terpenoid compounds by column chromatography ((eluent n-hexane: ethyl acetate: ethanol)) resulted in 25 eluates, and then merged based on the results of identification by TLC ((nhexane: ethyl acetate (8: 2)) and the color test reagent Lieburmann -Burchard produce 5 fraction groups. fraction D showed positive terpenoids with Rf 0.75 and the color purple with Lieburmann-Burchard reagent. study using 15 rabbits were divided into 5 groups: P1 (negative control), P2 (positive control) , P3 (5% of the test group), P4 (10% of the test group), P5 (test group 15%). each group was given the intracutaneous Staphylococcus aureus as 0,2ml on the backs of rabbits. Having symptoms of infection each group was given the test substance 3 times a day topically, the observed parameter is the diameter of the wound, and histopathological observations performed on days 3,6 and 9 Analysis of the results of research conducted using ANSIRA showed highly significant differences between groups (p <0.05). Then proceed with the analysis of the results of the analysis HSD test showed highly significant differences in the test group 5% to 10% of the test group and the test group 15%. Isolates terpenoid class of compounds from the seeds of papaya (Carica Papaya L.) with a concentration of 10% and 15% can inhibit the growth of Staphylococcus aureus. Keywords: Antibacterial, Staphylococcus aureus, male rabbits

The Effect of Young Papaya Seed Extract on The Motility and Abnormality of Male Mice Spermatozoes (Mus musculus)

Papaya fruit has many benefits, one of which is papaya seed can be used as antifertility drugs, especially young papaya seeds. Young papaya seeds contain secondary metabolites of alkaloid glycosides and proteolytic enzymes such as papain which can interfere with spermatogenesis. The study aims to determine the effect of young papaya seed extract on the sperm motility and abnormality of male mice (Mus musculus). Mice were randomly selected to represent 4 dose groups: control group (P0), extract 30mg/grBW (PI), extract 60mg/grBW (PII) and extract 90mg/grBW (PIII). Each treatment group was carried out with six repetitions. The treatment was given intravenously by injecting it in the tail of the mice for 15 consecutive days. The observed spermatozoa quality variables included a percentage of motile sperm and abnormal sperm. Data analysis using one-way ANOVA test with a significance level of 5% (0,000) and continued with the LSD (Least Significant Difference) with a 95% confidence level. The study on the Quality of spermatozoic showed a significant effect at the doses of 60mg/grBW (PII) and 90mg/grBW (PIII). The higher dose of young papaya seed extract increasingly affects the Quality of sperm. This research concluded that the extract of young papaya seeds has a significant impact on mice spermatozoa (Mus musculus) quality.

"Effect of Potassium Nitrate Priming and Different Growing Media on Papaya Seed Germination"

"Effect of Potassium Nitrate Priming and Different Growing Media on Papaya Seed Germination"

Hepatoprotective Effect of Papaya Seed Ethanol Extract on Rifampicin Isoniazid-induced Rats

Aims: Hepatotoxicity induced by anti-tuberculosis drugs, including rifampicin and isoniazid (AT- DILI, Anti Tuberculosis-Drug Induced Liver Injury), is an adverse reaction followed by significant morbidity. Several in vivo and in vitro research has confirmed that papaya seeds contain various non-essentials, minerals, and fiber. Carica papaya role in disease prevention through modulation of various processes, such as anti-inflammatory, anti-diabetes, immunomodulatory activity, and antioxidant activity, suggests a role in neutralizing free radical generation and ultimately preventing pathogenesis. This study aimed to determine the hepatoprotective effect of ethanol extract of papaya seeds on rifampicin and isoniazid- induced rats.
 Study Design: This study is experimental study.
 Methodology: The experimental animals in this study were divided into eight groups, including normal group, negative group 1, negative group 2, negative group 3, positive group, treatment group I (papaya seed ethanol extract dose of 100 mg/kgBW), treatment group II (papaya seed ethanol extract dose of 300 mg/kgBW), and treatment group III (papaya seed ethanol extract dose of 500 mg/kgBW), then the rats were dissected, and blood was taken for AST, ALT, ALP, GGT, and Bilirubin level measurements.
 Results: The results showed that papaya seed ethanol extract could reduce ALT, AST, ALP, GGT, and Bilirubin levels that were significantly different (P <0.05) than those in the negative control group.
 Conclusion: Flavonoid contains in the extract ethanol carica papaya has vita role to prevent the liver toxicity caused by isoniazid and rifampicin.

Proximate Composition and Phytochemical Constituents of Matured Carica papaya Seed Extracts

Aim: To investigate the proximate composition and phytochemical constituents of matured Carica papaya seed.
 Study Design: Proximate composition was determined on Carica papaya seed powder while extracts for phytochemical analysis were obtained, in turn using five different solvents of varying polarity, namely n-hexane, chloroform, ethyl acetate, methanol and water.
 Place and Duration of Study: Department of Biochemistry, Faculty of Basic Medical sciences, Bayero University Kano, Nigeria. The study was carried out between March- April, 2019.
 Results: The proximate composition of matured Carica papaya seed showed it contained crude fat (27.72%), carbohydrate (23.34%), crude fibre (21.25%), ash (10.25%), crude protein (9.65%) and moisture (7.34%). Qualitative phytochemical screening of Carica papaya seed extracts detected major phytochemicals except anthraquinones and quantitative analyses of these phytochemicals in all the five extracts showed that flavonoids was the most abundant phytochemical with 38.68%, 35.85%, 36.76%, 34.04% and 23.50% for methanol, aqueous, chloroform, n-hexane and ethyl acetate fractions respectively while tannins was the least abundant phytochemical with 0.03% for methanol extract, 0.04% for n-hexane extract, 0.09% for aqueous and ethyl acetate extracts, and 0.14% for the chloroform extract.
 Conclusion: Seeds of matured Carica papaya contain major nutrients that may be useful in nutrition. The presence of phytochemicals such as flavonoids, alkaloids and saponins in considerable quantities explained the medicinal activity of the plant material as encountered in its therapeutic uses.

Antihelmintic activity of extracts of papaya and avocado seed using Caenorhabditis elegans as model

BACKGROUND AND AIM: At present, the human being has sought different alternatives to improve their health, taking into account more naturalistic approaches to the treatment of problems related to the cardiac, digestive and nervous system. The latter can be related to parasites, which cause substantial deaths and morbidity in humans and diseases in livestock and domestic animals. Traditional medicine has been used to combat parasitic diseases, using tea from seeds of several fruits. METHODS: In this opportunity, the effectiveness in the antiparasitic activity of the aqueous extract of avocado and papaya seeds was evaluated using the soil nematode Caenorhabditis elegans as a model, through mortality as an endpoint of acute toxicity. Three solutions of each extract in distilled water (50, 75, 100%) were used to exposure L4-stage larvae of C. elegans wild type Bristol N2 for 24 h. RESULTS:Results indicate a high anthelmintic activity of the two seed extracts, with 86, 91, 94% of mortality with the respective concentration of the avocado seed extract and 63, 76, 90% with the extract of papaya seeds; showing a dose-dependent mortality curve. CONCLUSIONS:The above demonstrates the anthelmintic power of the avocado and papaya seeds and the ability of C. elegans to be used as a model to study anthelmintic activity due to the similarity of the physiological characteristics that shares with the parasitic nematodes that affect health of human beings. KEYWORDS: Toxicology, Food/nutrition, Health co-benefits

Performances of Crossbred Chickens Fed Fermented Papaya Leaf and Seed Powder at High Stocking Density

The study evaluated the influence of fermented papaya leaf and seed powder (FPLS) and/or multienzymes on the growth, physiology, antioxidant, and gut ecology of the Indonesian crossbred chicken (ICC) at high stocking density. Three hundred and seventy ICC were randomly allotted to LSD (chicks fed conventional feed at low stocking density), HSD (chicks fed conventional feed at high stocking density), HSD+mE (chicks fed conventional feed plus multienzyme at high stocking density), HSD+FPLS (chicks fed FPLS at high stocking density) and HSD+FPLS+mE (chicks fed FPLS plus multienzyme at high stocking density). Body weight and feed intake were determined weekly. Two ICC were taken from each pen (10 chicks per treatment group) at week 10 for sampling. The study was arranged based on a completely randomized design with five treatment groups, each consisted of five replicates. Except for performance, analysis of variance was conducted on two chicks from each replicate (10 chicks per treatment group). Chicks in LSD consumed more (p<0.05) feed and had a higher (p<0.05) feed conversion ratio. Platelet distribution width (PDW) was lower (p<0.05) in HSD, HSD+FPLS, and HSD+FPLS+mE chicks than in LSD chicks. Lymphocyte counts were lower (p<0.05) in HSD relative to HSD+mE chicks. Superoxide dismutase (SOD) was higher (p<0.05) in HSD and HSD+FPLS+mE chicks than in LSD, HSD+mE, and HSD+FPLS chicks. High-density lipoprotein was smaller (p<0.05) in HSD+mE chicks than in LSD, HSD, and HSD+FPLS chicks. Compared to the other treatment groups of chicks, HSD+FPLS chicks had a lower (p<0.05) albumin level. Creatinine level was lower (p<0.05) in HSD chicks than in the other group of chicks. Enterobacteriaceae counts were lower (p<0.05) in HSD+FPLS cecal content of chicks than in LSD and HSD chicks. The redness values of breast meats were lower (p<0.05) in HSD+FPLS chicks than the chicks in HSD+mE and HSD+FPLS+mE dietary treatments. LSD chicks had higher (p<0.05) redness values of thigh meat than the other treatment groups of chicks. LSD chicks also had higher (p<0.05) yellowness values than HSD+mE and HSD+FPLS+mE chicks. In conclusion, high stocking density resulted in mild stress conditions, as was demonstrated by the increased SOD and decreased PDW and redness meat values. A combination of FPLS and multienzyme ameliorated the adverse influence of high stocking density in ICC.

The Effect of Ethanol Extract of Carica papaya Seed towards Lipid Profile on Rats Induced Rifampicin and Isoniazid

Aims: Rifampicin one of the most commonly used front-line drugs in antituberculosis therapy, has been known to be hepatotoxic. The oxidative stress that is formed in the mitochondria due to rifampicin and isoniazid causes an imbalance in lipid metabolism. This study aims to determine the effect of lowering total cholesterol, triglyceride, and LDL levels and increasing HDL levels of papaya seed ethanol extract in rats induced by rifampin and isoniazid.
 Study design: This study is experimental study.
 Methodology: This study was divided into 9 groups including normal group, negative group 1, 2, 3, positive 1, 2, treatment group I (EECP 100 mg/kgbw), treatment group II (EECP 300 mg/kgbw), and treatment group III (EECP 500 mg/kgbw). Rifampicin (50 mg/kgbw), isoniazid (50 mg/kgbw), and EECP were given 28 days, on day 29 rats were dissected and blood was taken and the total cholesterol, triglyceride, LDL and HDL levels were measured.
 Results: The results showed that the ethanol extract of papaya seeds at a dose of 100 mg / kgbb, 300 mg / kgbb, and 500 mg / kgbw could reduce levels of total cholesterol, triglycerides, LDL and increase HDL levels. The dose of 500 mg / kgbw was not statistically significant (P> 0.05) with the normal group.
 Conclusion: Ethanol extract of Carica papaya has a potential effect of preventing the destruction of lipid metabolism effect by reducing the LDL, Trygliseride, Cholesterol Total, and increasing the level of HDL.

Influence of native endophytic bacteria on the growth and bacterial crown rot tolerance of papaya (Carica papaya)

The native plant microbiome is composed of diverse microbial communities that influence overall plant health, with some species known to promote plant growth and pathogen resistance. Here, we show the antibacterial and growth promoting activities of autoclaved culture metabolites (ACM) from native endophytic bacteria (NEB). These NEB were isolated from a papaya cultivar (var. Cariflora) that is tolerant to bacterial crown rot (BCR) caused by Erwinia mallotivora. In this cultivar, bacterial colonization in tissues recovering from the disease was observed before onset of tissue regeneration or ‘regrowth’. We further isolated and characterized these bacteria and were able to identify two culturable stem NEB related to plant endophytic genera Kosakonia sp. (ex. Enterobacter sp., isolate EBW), and to Sphingomonas sp. (isolate EBY). We also identified root NEB under genus Bacillus (isolates BN, BS, and BT). Inhibition assays indicated that ACM from these NEB promptly (within 18-30 h) and efficiently inhibited (60–65% reduction) E. mallotivora proliferation in vitro. When surface-sterilized papaya seeds were soaked in ACM from isolates EBY and EBW, germination was variably retarded (20–60% reduction) depending on plant genotype, but plant biomass accumulation was significantly stimulated, at around two-fold increase. Moreover, greenhouse experiments show that ACM from all isolates, especially isolate EBW, significantly reduced BCR incidence and severity in a susceptible genotype (var. Solo), at around two-fold. In general, our observations of pathogen antagonism and plant growth promotion leading to disease reduction, suggested the influence of native endophytic bacteria to increased fitness in plants, and tolerance against the re-emerging crown rot disease of papaya.

Efek Antibakteri Tumbuhan Pepaya (Carica Papaya L.) Terhadap Porpyromonas Gingivalis (Laporan Penelitian)

Background: Porphyromonas gingivalis is one of the bacteria that cause pulp necrosis, NaOCl is an irrigation solution that has become the gold standard in endodontic treatment, however NaOCl is toxic, so other alternative materials are needed. One usable material is papaya plant (Carica papaya L.) which has antibacterial properties. Objective: to know the differences in the activity of papaya leaves extract, papaya seed extract, and papaya fruit juice in inhibiting P.gingivalis. Methods: This study is an in vitro experimental laboratory of agar well diffusion method used papaya seeds extract, papaya leaves extract and papaya fruit juice in several consentrations (6.25%,12.5%,25%,50%, and 100%) and NaOCl 5,25% as positive control. Results: This study showed that the diameter of inhibitory zone against P.gingivalis was found on 2 concentrations of papaya seed extract, a concentration of 100% with an average 23,43 mm and concentration of 50% with an avarege 16,43 mm. The inhibition zone test of papaya leaves extract and papaya fruit juice against P.gingivalis was not found around the well. Conclusion: Papaya seed extract was able to inhibit P.gingivalis in certain concentration, while papaya leave extract and papaya fruit juice were not able to inhibit the growth of P.gingivalis.

Sub-fractions from Carica Papaya Seed Extracts Can Prevent Potassium Bromate- induced Changes in Activities of Renal Brush Border Membrane Enzymes and Some Enzymes of Carbohydrate Metabolism in the Kidney of Rats

Aim: To investigate the effect of the chromatographic fractions of Carica papaya seed on KBrO3 –induced reduction in the activities of renal brush border membrane (BBM) marker enzymes and the changes in activities of some enzymes of carbohydrate metabolism in the kidney of rats.
 Study Design: twenty male Wistar rats were divided into four groups, five rats per group; normal control, KBrO3 control, papaya fraction control and KBrO3 group administered with 126mg/kg body weight of the most active fraction of partially purified methanol extract of C. papaya for 48 hours.
 Place and Duration of Study: Department of Biochemistry Laboratory, Faculty of Basic Medical Sciences, Bayero University Kano, Nigeria.
 Methodology: The activities of renal BBM marker enzymes: γ-glutamyl transferase, alkaline phosphatase, maltase and leucine aminopeptidase were assayed in homogenates of renal cortex and medulla, and in brush border membrane vesicle (BBMV) isolated from cortex using standard methods. Furthermore, activities of the following enzymes representing different pathways of carbohydrate metabolism were determined in renal homogenates: hexokinase (HK), lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose 6-phosphatase (G6P), fructose 1,6-bisphosphatase (FBP), glucose 6-phosphate dehydrogenase (G6PD) and malic enzyme (ME).
 Results: KBrO3 administration significantly (P<0.05) decreases the activities of all the BBM marker enzymes in renal homogenates and BBMV. It also decreases the activities of MDH, G6P, FBP and G6PD, and significantly increases (P<0.05) that of HK, LDH and ME in renal homogenates however co-administration of most active fraction of C. papaya seed prevented all the KBrO3 -induced changes in these biochemical parameters.
 Conclusion: Chromatographic fractions of C. papaya seed extract possesses potent phytochemicals that could prevent KBrO3 –induced reduction in activities of renal BBM marker enzymes and the changes in enzymes of carbohydrate metabolism studied and therefore could be analyzed further to isolate the bioactive compounds.

Effectiveness of combination of Moringa Leaf Extract (Moringa oleifera Lamk.) and Papaya Seed Extract (Carica papaya L.) in reducing blood sugar levels of diabetic rats

Introduction: Moringa leaf extract and papaya seed extract contain flavonoids that can lower blood sugar levels. Aims: The purpose of this study was to determine the antidiabetic effectiveness of moringa leaf extract and papaya seed extract and the effective dose of moringa leaf extract and papaya seed extract in reducing blood sugar levels. Methods: This study used 24 diabetic rats. Diabetes was induced by giving rats glucose 10% for four days. Measurement of blood sugar levels was carried out on the 0th, 5th, 8th, and 15th days. The pre-test (T1) and post-test (T2 and T3) blood sugar levels were measured, as well as the percentage of reduction at T2 and T3. Results: The result was that the treatment group with an extract combination of 700:500 showed decreased blood sugar levels on day 15 (57.24%), indicating antidiabetic effectiveness of the extract combination.

Study of effect of papaya seed on reproductive performance in Nile Tilapia (Oreochromis niloticus)

The present study was conducted to examine the ability of papaya (Carica papaya) seeds in reducing the reproductive performance of Nile tilapia through gonadal sterilization. In the first phase, 9 days fry of Nile tilapia were reared in 50-L size aquaria and fed with normal feed (T1) and papaya seed powder mixed feed @ 50 (T2), 100 (T3), and 150 (T4) g/kg diet at the rate of 5 percent of body weight for 30 days. After 30 days of treatment, 50 fish from each group were reared in outdoor hapa for 6 months with normal feed, and gonadal status was observed. In the second phase, matured fish from both the control and papaya seed treated group (T4@150 g/kg diet) were reared in two separate hapas for the next 3 months to observe their reproductive performance. Results showed that papaya seed feeding had no adverse effect on growth, survival, and water quality during the treatment period. The gonadosomatic index of both males and females was significantly lower in the papaya seed treated group (0.1±0.0 percent) than control (0.2±0.01 percent). The number of fish spawned per week was significantly lower in the papaya seed treated group (1.2±0.2) compared to the control group (3.6±0.3). The number of eggs per g female was significantly lower in the papaya seed treated group compared to the control group. The fertilization rate was significantly lower in the papaya seed treated group (93.6±1.1 percent) compared to the control group (98.2±1.3 percent) (P˂0.05). This study demonstrated that feeding papaya seeds at the dose of 150 g/kg diet (15 percent) can reduce the reproductive performance of Nile tilapia.

Deshelled Carica papaya Seeds as Natural Coagulant for Improvement Quality of River Water

This study evaluated the efficiency of deshelled papaya seeds as the natural coagulant in improving quality of river water. The deshelled papaya seeds biomass was mainly characterized for functional groups using FTIR, surface charge and protein content. Coagulation and flocculation tests were conducted using batch test for various dosages and pH values. The optimum dosage and pH were then tested in treating river of Class III to Class V. The functional groups in deshelled papaya seeds were O-H, C=O and C-O groups. The surface charge and protein content was +0.4 meq/g and 363 mg/g, respectively. The deshelled papaya seeds coagulant was able to remove turbidity up to 87.6 and 88.3% for river of Class III and Class IV, respectively, as well as 57.6 and 62.1% of total coliform and escherichia coli, respectively, from Class III river at dosage 196 mg/L and pH 4. This study demonstrated that the deshelled Carica papaya seeds-derived coagulant is capable in treating common pollutants of river and simultaneously shows antibacterial properties.

Potential of Carica papaya Seed-Derived Bio-Coagulant to Remove Turbidity from Polluted Water Assessed through Experimental and Modeling-Based Study

It is important to develop renewable bio-coagulants to treat turbid water and efficient use of these bio-coagulants requires process optimization to achieve robustness. This study was conducted to optimize the coagulation process using bio-coagulant of deshelled Carica papaya seeds by employing response surface methodology (RSM). This bio-coagulant was extracted by a chemical-free solvent. The experiments were conducted using the Central Composite Design (CCD). Initially, the functional groups and protein content of the bio-coagulant were analyzed. The Fourier Transform Infrared Spectroscopy analysis showed that the bio-coagulant contained OH, C=O and C-O functional groups, which enabled the protein to become polyelectrolyte. The highest efficiency of the bio-coagulant was obtained at dosage of 196 mg/L, pH 4.0 and initial turbidity of 500 NTU. At the optimum conditions, the bio-coagulant achieved 88% turbidity removal with a corresponding 83% coagulation activity. These findings suggested that the deshelled Carica papaya seeds have potential as a promising bio-coagulant in treating the polluted water.