The Effect of Young Papaya Seed Extract on The Motility and Abnormality of Male Mice Spermatozoes (Mus musculus)

Tannya Efritzka Louise Calame,Kusmiyati Kusmiyati,I Wayan Merta

doi:10.29303/jpm.v16i4.2790

Abstract

Papaya fruit has many benefits, one of which is papaya seed can be used as antifertility drugs, especially young papaya seeds. Young papaya seeds contain secondary metabolites of alkaloid glycosides and proteolytic enzymes such as papain which can interfere with spermatogenesis. The study aims to determine the effect of young papaya seed extract on the sperm motility and abnormality of male mice (Mus musculus). Mice were randomly selected to represent 4 dose groups: control group (P0), extract 30mg/grBW (PI), extract 60mg/grBW (PII) and extract 90mg/grBW (PIII). Each treatment group was carried out with six repetitions. The treatment was given intravenously by injecting it in the tail of the mice for 15 consecutive days. The observed spermatozoa quality variables included a percentage of motile sperm and abnormal sperm. Data analysis using one-way ANOVA test with a significance level of 5% (0,000) and continued with the LSD (Least Significant Difference) with a 95% confidence level. The study on the Quality of spermatozoic showed a significant effect at the doses of 60mg/grBW (PII) and 90mg/grBW (PIII). The higher dose of young papaya seed extract increasingly affects the Quality of sperm. This research concluded that the extract of young papaya seeds has a significant impact on mice spermatozoa (Mus musculus) quality.

Highlights

of which is papaya seed can be used as antifertility drugs especially young papaya seeds
This is because young papaya seeds contain secondary metabolites of alkaloid glycosides
such as papain which can interfere with the process of spermatogenesis

Summary

METODE PENELITIAN Metode ini menggunakan Penelitian

Rancangan Acak Lengkap (RAL) [9]. Dilakukan bulan Februari-Maret 2021 di Laboratorium Kimia dan Biologi FKIP Universitas Mataram. Kemudian ekstrak biji pepaya tersebut ditimbang sesuai dengan dosis perlakuan yaitu 30 mg/grBB, 60 mg/grBB, dan 90 mg/grBB. Setelah itu sperma diambil dari cauda epididimis, kemudian ekstrak biji pepaya tersebut ditimbang sesuai dengan dosis perlakuan yaitu 30 mg/grBB, 60 mg/grBB, dan 90 mg/grBB. Selanjutnya dianalisis dengan cara menghitung rata-rata persentase motilitas dan abnormalitas spermatozoa pada tiap kelompok percobaan. Dilanjutkan dengan analisis statistik menggunakan Uji One-way anova yaitu untuk mengetahui adanya pengaruh ekstrak biji pepaya muda terhadap kualitas spermatozoa mencit jantan (Mus musculus), pada tingkat kepercayaan 95% dengan signifikan. HASIL DAN PEMBAHASAN Hasil pengamatan motilitas rata-rata pengaruh ekstrak biji pepaya muda terhadap kualitas spermatozoa mencit jantan (Mus musculus) terdapat pada tabel 1 yang menunjukkan bahwa terdapat dua puluh empat hewan uji dari empat kelompok yang berbeda dan memiliki rata-rata abnormalitas spermatozoa yang berbeda

Motilitas Spermatozoa

Sum of Squares

Abbnormalitas Spermatozoa

Sum of Df Mean

Pencegahan Kekerasan Seksual Pada

Varietas Thailand Terhadap Penurunan