The effect of processing parameters on the surface roughness parameter Rz (mean peak-to-valley height) in CNC machining of Anatolian chestnut (Castenia sativa Mill.) wood species used in the woodworking and furniture industry was investigated. The machining conditions were determined to obtain the lowest Rz value. The experi-ments were carried out at the speeds of 8000, 12000, and 16000 rpm and the feed rates of 1000, 1500, and 2000 mm/min with 3 and 4 machining layers by using two different cutters with a diameter of 8 mm. Rz measurements were made on the obtained surfaces using the needle scanning method according to TS EN ISO 21920-2 (2021). In the measurements perpendicular to the fibers, the lowest Rz values were obtained with cutter type 2 (the cutter of the three cutting edge straight end mill), at 16000 rpm speed, 1000/2000 mm/min feed rate, and four machining layers. In the measurements made parallel to the fibers, the lowest Rz values were obtained at the cutter type 2 (the cutter of the three cutting edge straight end mill), at the speed of 16000 rpm and the feed rate of 1000/2000 mm/min and 4th step-down. A smooth surface (lower Rz value) was obtained with fourth cuts (step down, cut layer) instead of third cuts.