Aims: To determine serum leptin concentration, insulin resistance and the relationship with other factors in pre-diabetes population. Methods: A total 275 prediabetic subjects were included in this study. Collected data were: anthropometric characteristics, blood pressure, family history and history of metabolic diseases. Fasting blood samples were obtained in the early morning and assayed for serum leptin, blood glucose, HbA1c, insulin, lipid profile and hsCRP. Results: Serum leptin levels were significant higher (4.57 (0.04 – 61.57) ng/mL) in prediabetes group. The prevalence of hyperleptinemia was 41% and significant associated with female gender, overweight, viseral obesity, insulin, HOMA-IR and hsCRP. The prevalence of insulin resistance was 61% and leptin was an independent risk factor of insulin resistance. Conclusion: Prediabetic patients have higher leptin concentration than normal subjects and hyperleptinemia is associated insulin resistance.